• The Korean Journal of Mycology
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• v.21 no.2
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• pp.120-126
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• 1993

Morphological characteristics in the anamorphic states were described in three species of powdery mildew fungi, Microsphaera akebiae, M. picrasmae and M. schizandrae, which were parasitic on Akebia quinata, Picrasma quassioides, and Schizandra chinensis, respectively. All species were flexuous in foot-cells of conidiophores, producing conidia singly on conidiophores, devoid of fibrosin bodies in conidia, and producing germ tubes on the end of conidia.

• Korean Journal of Weed Science
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• v.14 no.3
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• pp.223-227
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• 1994

Basis for differential response of rice and E. crus-galli to cyhalofop-butyl ester (R-butyl 2-(4-(4-cyano-2-fluoro-phenoxy)phenoxy)propionate) was anatomically compared. Cell division in the growing point of E. crus-galli was severely inhibited by the herbicide, leading to malformation and irregular arrangement of cells. The inhibitory symptom of rice by cyhalofop-butyl ester appeared to be very similiar to that of E. crus-galli although its injury was negligible in rice when applied at the rate of 180ppm.

• Korean Journal of Food Science and Technology
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• v.14 no.4
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• pp.336-341
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• 1982

The histochemical examination of Undaria pinnatifida Laver were conducted with light microscope and electron microscope. The results obtained were as follow: 1. Undaria pinnatifida frond were composed with epidermis, cortex and medulla. But the cutting section of Undaria pinnatifida Laver showed that only the epidermal cell were bound to each other. The cortex and medulla of the frond were destroyed during U.P. Laver process. 2. To identification of bind material of U.P. Laver, which were treated with sodium carbonate solution for extraction of alginic acid and reacted with Periodic Acid Schiff(PAS) reagent. And the PAS reaction result was negative by light microscope observation. On this result, we found out that the alginic acid has the binder role of U.P. Laver. 3. Also, the bind structure of U.P. Laver were observed by electron microscope and could well find out the epidermal cell wall and bind position of alginic acid, which were could not observed by light microscope.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.3
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• pp.394-398
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• 2002

Acetic acid bacteria have been isolated from running high-acid vinegar fermentation. The color of the isolated colony was beige-yellowish. Isolated cell was rod-shaped, small, pale, absolutely aerobic and gram-negative. Microscopically the cells appeared as non-motile and non-flagellated, preferentially occuring in pairs. The optimum temperature and pH for culture were 30$\^{C}$ and 2.7, respectively. The strain was able to grow in the presence of acetic acid, ethanol and glucose. Ethanol was oxidized to acetic acid which was not oxidized any more. The isolated strain utilized glucose, fructose, maltose, sucrose, mannitol and sorbitol as carbon source. Cellulose formation was not detected on bouillon. The DNA (G +C) content of isolated strain was determined to be 56.2 mol%. The strain isolated from industrial vinegar fermentation was identified as Gluconacetobacter europaeus.

• Research in Plant Disease
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• v.18 no.1
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• pp.45-48
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• 2012

In early August of 2011, following a prolonged period of cool and moist weather, several trees of boxelder maple (Acer negundo) planted in Mt. Soyo located in Dongducheon, Korea, were found to be heavily damaged by premature defoliation with zonate leaf spot symptoms. Numerous number of cone-shaped, white sporophores (conidia) were observed on lesions of the abaxial leaf surface. The morphological characteristics of conidia are of typical Cristulariella moricola, which was supported by analyzing sequences of internal transcribed spacer region of ribosomal DNA. Pathogenicity of the fungus was proved by artificial inoculation in the condition of relative humidity 100% and $18{\pm}2^{\circ}C$. This is the first report of the occurrence of zonate leaf spot caused by infection of C. moricola on A. negundo in Korea.

• Journal of the Korea Convergence Society
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• v.10 no.6
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• pp.65-71
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• 2019

The propose of this study has been conducted to examine expression of c-Myc and Thymosin-${\beta}4$ in liver cirrhosis model from liver fibrosis and For the method of study, the experiment was conducted in 2 groups; liver cirrhosis model experiment group due to liver fibrosis and control group with distilled water. This study outcome showed that liver cirrhosis model experiment group had significantly higher expression of c-Myc and Thymosin-${\beta}4$. with changes to hepatic tissue of special staining and electron microscopy. In conclusion, in clinical tests regarding liver function, molecular evaluation of c-Myc and Thymosin-${\beta}4$ and their expression along with serological change and histological assessment can be utilized as a reference for diagnosing liver disease for prevention and diagnosis of the disease, Based on this research in the future, we will carry out an in-depth study by adding the types of experimental groups and related genes.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.119-119
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• 2021

최근 국내에서는 이상기후변화로 인해 수환경 변화가 일어나고 있으며, 이로인해 하천이나 저수지에 조류의 과대성장이 빈번히 발생되고 있다. 조류의 과대성장으로 인해 조류가 생산하는 독성물질, 이취미 물질은 수질을 악화 및 생태계에 큰영향을 미치는 실정이다. 또한, 조류는 하천, 저수지에 넓은 분포로 발생하게 되며, 현재 조류 조사방법은 다양한 계측장비를 통해 조사를 진행하고 있으나, 조류를 직접 채취하여 검경하거나, 보트 또는 조사선에 센서를 부착하여 측정하기 때문에, 점 또는 선단위의 간혈적 조사가 진행된다. 따라 많은 인력과 시간이 소요된다. 국내에선 인력과 시간을 줄이기 위해 최근 위성영상과 드론을 활용한 조류 원격탐사 모니터링에 대한 연구가 많이 진행되고 있다. 본 연구에서는 안동댐 인근 예안교에서 드론과 초분광센서를 이용하여 초분광데이터를 수집 및 조류 맵핑을 진행하였다. 사용된 드론은 DJI사의 RGB드론과 M-600Pro를 사용하였으며, 초분광 센서는 CORNING사의 microHSITM 410 SHARK를 이용하였으며, 파장 400-1000 nm에서 NIR(visNIR)파장을 분석할 수 있다. 드론에 짐벌을 장착하여 초분광센서를 수표면과 평행하게 두고 촬영 및 영상을 수집하였다. 방사보정을 하기위해 영상을 촬영 구간에 방사 보정용 반사천을 두고 동시간에 같이 촬영 하여 방사보정을 진행하였다. 본 연구에서는 초분광센서와 드론을 활용하여 조류 맵핑에 대한 연구를 하기위해 시료를 채취하여 검경결과와 비교분석을 하였으며, 초분광영상 분석을 통해 조류 최적밴드비를 산정하고 조류 맵핑을 진행하여 촬영구간에 대한 2차원 조류 맵핑 제시하여 하천에 적용하고자 한다.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.20-20
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• 2022

최근 국내 기후변화와 산업화로 인한 오염물 배출량 증가로 인해 하천 및 호소에 남조류 과대 성장이 빈번히 발생한다. 과거에 비해 녹조현상의 빈도가 잦아졌으며, 지속기간이 증가하였다. 조류 발생 시 현장에서 채수한 샘플을 통해 검경 및 chlorophyll-a 분석을 진행하여 조류의 정성·정량적 평가를 진행한다. 이러한 분석결과는 녹조 발생에 대한 기초자료로 활용되고 있다. 하지만 현장 시료 샘플을 통한 조류 검경의 경우 단일지점에 대한 분석으로 인해 하천 및 호소의 전체적인 대표성을 나타내기엔 한계가 있고, 많은 인력과 시간이 소요된다. 또한 chlorophyll-a는 녹조류와 높은 관련성이 있어 유해남조류에 대한 집중 분석에는 한계가 발생한다. 이러한 한계점을 통해 국내·외로 조류 연구에 분광 스펙트럼을 활용한 원격탐사 기법 적용되고 있으며, 남조류 농도를 분석하기 위해 남조류의 보조 색소인 phycocyanin을 활용한 연구가 진행되고 있다. 본 연구에서는 분광스펙트럼을 활용한 phycocyanin 분석을 진행하였다. 분광 스펙트럼을 측정하기 위해 점(point) 단위의 고해상도 분광방사계를 활용하였으며, phycocyanin 측정을 위해서 형광 센서를 활용하였다. 현장에서 형광센서 및 분광방사계를 동시에 측정하여 데이터를 취득하였으며, 분광 스펙트럼 특성상 노이즈 및 이상치가 발생하기 때문에 전처리 과정을 통해 이를 보완하였다. phycocyanin 분석을 위해 다중 스펙트럼을 활용한 분광지수를 활용했으며, 이를 통해 phycocyanin 분석에 있어 최적의 분광지수를 제시하고자 한다. 본 연구 결과는 고해상도 점(point)단위 분석으로 향후 선단위, 면단위 하천 조류-스펙트럼 분석에 있어 기초자료로 활용될 것으로 판단된다.

• Restorative Dentistry and Endodontics
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• v.30 no.2
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• pp.112-120
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• 2005

This study was done to evaluate whether there were any differences in microleakage of class V composite restorations according to restoration site and cavity size. Total sixty-four restorations were made in molar teeth using Esthet-X. Small ($2\;{\times}\;2\;{\times}\;1.5\;mm$) and large ($4{\times}2{\times}1.5\;mm$) restorations were made at the buccal/lingual surface and the proximal surface each. After 1,000 times of thermocycling ($5^{\circ}\;-\;55^{\circ}C$), resin replica was made and the percentage of marginal gap to the whole periphery of the restoration was estimated from SEM evaluation. Thermocycled tooth was dye penetrated with $50\%$ silver nitrate solution. After imbedding in an auto-curing resin, it was serially ground with a thickness of 0.25 mm. Volumetric microleakage was estimated after reconstructing three dimensionally. Two-way ANOVA and independent T-test for dye volume, Mann-Whitney U test for the percentage of marginal gap, Spearman's rho test for the relationship between two techniques were used, The results were as follows : 1. The site and size of the restoration affected on the microleakage of restoration. Namely, much more leakage was seen in the proximal and the large restorations rather than the buccal/lingual and the small restorations. 2. Close relationship was found between two techniques (Correlation coefficient = 0.614/ P = 0.000). Within the limits of this study, it was noted that proximal and the large restorations leaked more than buccal/lingual and the small restorations. Therefore, it should be strictly recommended large exposure of margins should be avoided by reducing unnecessary tooth reduction.

• Tuberculosis and Respiratory Diseases
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• v.39 no.6
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• pp.517-525
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• 1992

Background: Since its development by Saiki et al, polymerase chain reaction (PCR) has been very useful in various fields of molecular biology. PCR can be used for the detection of a very small amount of microbial agent, and is especially useful in those patients who are difficult to diagnose microbiologically or serologically. Mycobacterium tuberculosis is a very slowly growing organism and AFB staining frequently shows false negative results, and therefore PCR would be a very rapid, easy, and sensitive diagnostic method for the diagnosis of Mycobacterium tuberculosis. Method: To compare PCR with conventional methods in diagnosing Mycobacterium tuberculosis in sputum, we used sputa of patients who visited or were admitted to Seoul National University Hospital. The amplification targets were 383 base pair DNA, a part of 2520 base pair DNA encoding 65 kD Mycobacterium tuberculosis specific protein (the primers are TB-1, -2), and 123 base pair DNA, a part of IS6110 fragment, which multiple copies are known to exsist PCR one genome (the primers are Sal I-1, -2). We also requested AFB staing and culture to the lab of Seoul National University Hospital with the same sample and compared the results. Results: 1) Using TB-1, -2 primers, PCR was positive in 73.1% (19/26) of culture positive sputa, in 12.5% (1/8) of culture negative. but clinically diagnosed tuberculous sputa, and was negative in all sputa of patients who were clinically diagnosed as non-tuberculous etiology. 2) Using Sal I-I, -2 primers, PCR was positive in 94.1% (32/34) of culture positive sputa, in 23.1% (6/26) of culture negative, but clinically diagnosed tuberculous sputa, and was negative in 87.5% (14/16) of sputa from patients who were clinically diagnosed as non-tuberculous etiology. Conclusion: PCR could be a very rapid, sensitive and specific method for the diagnosis of Mycobacterium tuberculosis in sputa, and further studies should be followed for the development of easier method.