Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Zonate Leaf Spot of Acer negundo Caused by Cristulariella moricola in Korea

Cristulariella moricola에 의한 네군도단풍 둥근무늬낙엽병

  • Cho, Sung-Eun (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Park, Ji-Hyun (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Lee, Sang-Hyun (Division of Forest Diseases and Insect Pests, Korea Forest Research Institute) ;
  • Lee, Hyang-Burm (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 조성은 (고려대학교 환경생태공학부) ;
  • 박지현 (고려대학교 환경생태공학부) ;
  • 이상현 (국립산림과학원 산림병해충연구과) ;
  • 이향범 (전남대학교 응용생물공학부) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Received : 2011.11.24
  • Accepted : 2012.03.09
  • Published : 2012.04.30
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Abstract

In early August of 2011, following a prolonged period of cool and moist weather, several trees of boxelder maple (Acer negundo) planted in Mt. Soyo located in Dongducheon, Korea, were found to be heavily damaged by premature defoliation with zonate leaf spot symptoms. Numerous number of cone-shaped, white sporophores (conidia) were observed on lesions of the abaxial leaf surface. The morphological characteristics of conidia are of typical Cristulariella moricola, which was supported by analyzing sequences of internal transcribed spacer region of ribosomal DNA. Pathogenicity of the fungus was proved by artificial inoculation in the condition of relative humidity 100% and $18{\pm}2^{\circ}C$. This is the first report of the occurrence of zonate leaf spot caused by infection of C. moricola on A. negundo in Korea.

저온다습한 날씨가 지속된 후의 2011년 8월 초순에 경기도 동두천시 소요산에서 겹둥근무늬와 조기낙엽 증상을 나타내는 네군도단풍이 발견되었다. 병든 잎의 뒷면에서 원뿔 모양이며 흰색의 분생포자가 다수 관찰되었다. 현미경 검경을 통하여 형태적 특징을 검토한 결과, 이 증상의 원인균은 Cristulariella moricola로 동정되었다. 상대습도 100% 및 $18{\pm}2^{\circ}C$의 조건에서 인공접종을 통하여 이균의 병원성을 증명하였다. 한편, 분리 균주의 internal transcribed spacer 영역의 염기서열을 분석하고 NCBI에 등록된 균의 염기서열과 비교함으로써 이 균이 C. moricola임을 증명하였다. 네군도단풍에 C. moricola의 감염에 의해 발생한 둥근무늬낙엽병은 우리나라에서 최초의 기록이다.

Keywords

References

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