• Korean Journal of Environmental Agriculture
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• v.37 no.3
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• pp.207-212
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• 2018

BACKGROUND: Recently, the need for a method to cultivate 'Haryejosaeng' Satsuma mandarin has been increasing. However, there is limited information available as this is a new Satsuma mandarin cultivar, which was bred by the RDA in 2004. Many farmers who cultivate this cultivar follow the cultivation method similar to that used for 'Miyagawa' Satsuma mandarin, and suffer low production of optimum-sized fruits. METHODS AND RESULTS: This study was conducted to find out the optimum ratio of leaf-to-fruit for the stable production of high quality 'Haryejosaeng' Satsuma mandarin fruits in a non-heated plastic film house. Seven-year-old 'Haryejosaeng' Satsuma mandarin trees were used in the study. Before the treatment, the leaf-to-fruit ratio ranged from 5.7 to 17.9. The treatments included 10, 20, 30, and 40 leaves per fruit. The fruits were removed if over fruiting was observed at day 60 after full bloom. We investigated the fruit size and quality on the day of harvest. Flowering and fruiting patterns in each treatment were recorded for the following year. In the experiments, the flower-to-leaf ratio was 1.12 to 1.74. As the leaf-to-fruit ratio decreased, the fruit size and weight also decreased. Contrarily, the higher the ratio of leaf-to-fruit, the higher fruit size and weight were. It was noted that the ratio of 20:1 was ideal to produce the M grade optimum-sized Satsuma mandarin fruits on the day of harvest. However, higher ratio might result in fruits weighting above 100 g. There was no difference among the treatments in terms of fruit quality, such as total soluble solid contents, titratable acid, and color. In the subsequent years, flowering and fruiting in the treatments were lowered when the leaf number per fruit was 10, but they were improved when the leaf number per fruit was above 20. CONCLUSION: Based on the above results, the optimum ratio of leaf-to-fruit was found to be 20:1 for flowering and fruiting of 'Haryejosaeng' Satsuma mandarin. It is important that optimum ratio of leaf-to-fruit is set as a standard to produce good grade and quality of 'Haryejosaeng' Satsuma mandarin fruits.

• Korean journal of applied entomology
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• v.48 no.4
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• pp.535-539
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• 2009

This study was conducted to identify plant-parasitic nematodes and determine the distribution pattern of the nematode species in citrus fruit orchards in Korea from April to August, 2008. Plant-parasitic nematodes were found in 139 among 178 soil samples. Tylenchulus semipenetrans was collected from 85 out of 94 Citrus unshiu orchards examined (90.4%). C. junos orchards infested with T. semipenetrans were 60 out of 84 (71.4%). However, proportion of dominant species between the two was different. At domestic Citrus orchards, it proved that T. semipenetrans was the most important plant-parasitic nematode from this study. The overall frequency of the plant-parasitic nematodes was positively correlated with the cultivation years. More alkaline soils tended to harbor more T. semipenetrans. However the correlation coefficient between the two was very low.

• Journal of Life Science
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• v.20 no.11
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• pp.1691-1696
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• 2010

Neohesperidin is a natural new nutrition sweetener, widely existing in plants of dry citrus peel, which can be derived from extraction. Since the sweetness is 1,300-1,500 times greater than that of sugar, neohesperidin are widely used in fruit juices, wines, beverages, bakeries and pharmaceutical formulations, and are particularly suitable for consumption by diabetic patients. However, the yield of extraction from citrus peel waste is very low. In this study optimal yield conditions were determinedusing response surface methodology (RSM) in order to increase the neohesperidin extraction yield. The critical factors for maximum extraction yield were selected extraction pressure ($x_1$), extraction time ($x_2$), and concentration of ethanol ($x_3$). As a result, the extraction yield was improved when the extracting pressure increased. The extraction yield also increased in a time-dependent manner. When adding ethanol as an assistance solvent to the supercritical carbon dioxide, extraction yield was increased as more ethanol concentration was added. Finally, the extraction yield of neohesperidin was improved to about 162.22% compared to ethanol extraction as a conventional method.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 2001.11a
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• pp.167-176
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• 2001

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2016.10a
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• pp.299-299
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• 2016

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2016.10a
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• pp.300-300
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• 2016

• Korean Chemical Engineering Research
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• v.53 no.6
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• pp.717-722
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• 2015

D-limonene included in citrus fruits is obtainable to extract essential oil as well as separate the oil ingredient. Soxhlet extraction, a type of SDE (Simultaneous steam Distillation and solvent Extraction), was used to extract limonene from tangerine peel. HPLC analysis was performed to quantify extracted d-limonene by using reversed-phase HPLC column. Results of HPLC analysis showed that the optimal extraction time was 2 hours in any solvent, and the extracted amounts of d-limonene in tangerine peel (per g tangerine peel) were 7.77 mg, 0.49 mg, and 0.28 mg in ethyl alcohol, n-hexane, and ether. Because yield was the highest in using ethyl alcohol as a solvent, polarity is stronger factor to effect on yield of extraction than boiling point.

• Journal of the Korea Organic Resources Recycling Association
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• v.27 no.3
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• pp.5-13
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• 2019

This study is conducted to investigate the effects of anaerobic treatments of swine manure slurry alone and combination of livestock manure slurry and fruit pomace on biogas production. Anaerobic co-digestion was evaluated in mesophilic tank reactors for 96 day-incubation period. The organic matter loading of anaerobic digestion was 1 kg of volatile solids(VS) per $1m^3{\cdot}day$. The highest methane production was achieved from the combination of swine manure slury and mandarin pomace(70:30) treatment, whereas the lowest daily and cumulative methane yields was observed in swine manure slurry alone treatment. More than two-fold increase in bio-gas and methane production was obtained by combination of livestock manure slurry and mandarin pomace treatment, compared to the swine manure slurry alone treatment. The co-digestion of livestock manure and fruits pomace has advantages to enhance the production of methane gas, compared to digestion of swine manure slurry alone.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.10
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• pp.1422-1429
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• 2016

Kombucha is a slightly sour beverage fermented by symbiotic micro-organisms, including bacteria and yeasts. In this study, we examined the biological activities of citrus Kombucha (CK) produced by addition of citrus extract to original Kombucha (K). After fermentation for 10 days, radical scavenging activity examined by ABTS and DPPH assays increased by approximately 20% compared to that of K. Moreover, content of total phenolic compounds significantly increased by 60% compared to that of K. Cell proliferation assays utilizing MTT showed that CK treatment significantly inhibited growth of bladder cancer cells, T-24 and 5637, in a dose-dependent manner with $IC_{50}$ values of 4 and 7 mg/mL, respectively. Annexin V staining showed that CK treatment led to apoptosis of cells in a dose-dependent manner. T-24 cells were more sensitive to CK treatment than 5637 cells, as 8 mg/mL of CK resulted in 97% apoptosis of T-24 cells. Western blotting showed that CK treatment led to up-regulation of apoptotic proteins, including caspases-3, -8, -9, and PARP, in bladder cells not in K-treated cells. Taken together, these results demonstrate that CK may be developed as a functional beverage.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.12
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• pp.1838-1842
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• 2016

Kombucha (K) is a fermented beverage made from black tea by symbiotic micro-organisms of bacteria and yeasts. To enhance bio-activities of K, we produced citrus/green tea Kombucha (CK) by adding extracts of citrus peel and green tea obtained from Jeju Island. ORAC assays showed that anti-oxidative capacity of CK increased by approximately 3-folds compared to K. We examined anti-cancer properties of extracts from citrus peel and/or green tea using 5637 human bladder cancer cells and B16F10 murine melanoma cells. Proliferation of B16F10 cells was markedly inhibited at concentrations higher than $10{\mu}L/mL$. At a concentration of $20{\mu}L/mL$, anti-cancer activities of extracts were in the order of citrus peel< green tea< combination of both. Interestingly, a combination of both extracts showed a synergistic effect on inhibition of growth of cancer cells. CK fermented with citrus peel and green tea extracts showed enhanced anti-cancer activity compared to K. Cytotoxicity of CK on RAW 264.7 murine macrophages was negligible up to $100{\mu}L/mL$. Taken together, these results indicate that citrus Kombucha is safe to be developed as a functional beverage.