• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.5
• /
• pp.266-273
• /
• 2020

This study was undertaken to determine the effects of diet supplementation with complex probiotics (CPB), on growth performance, nutrient digestibility, blood metabolites, noxious gas, and fecal microflora in weaning pigs. On the basis of body weight, a total of 234 weaned pigs (Landrace×Yorkshire×Duroc, 6.14±0.78kg) were randomly allotted to 3 treatments and 6 replicates (13 pigs per pen). The experimental diets were fed in a meal form for 28 days (days 0-14, PhaseI, and days 15-28, PhaseII). The dietary treatment groups were as follows: T1 (basal diet), T2 (T1+0.13% CPB) and T3 (T1+0.25% CPB). The CPB supplement contained Bacillus subtilis 1.0×10⁶ CFU/g, Enterococcus faecium 1.0×10⁶ CFU/g, Saccharomyces cerevisiae 1.0×10⁶ CFU/g, Bacillus licheniformis 3.0×10⁸ CFU/g, and Bacillus polyfermenticus 3.0×10⁸ CFU/g. Pigs fed the T3 diet showed an increase (p<0.05) in the overall average daily gain and average daily feed intake, increased (p<0.05) crude protein digestibility in PhaseI, and greater (p<0.05) dry matter and gross energy digestibility in PhaseII. Supplementation of CPB had no effect on the blood profile. Furthermore, pigs fed the T3 diet had lower (p<0.05) NH₃ emission and overall count of fecal Clostridium spp. In conclusion, we believe that CPB supplementation has a beneficial effect on growth performance, nutrient digestibility, noxious gas, and fecal microflora in weaning pigs.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.21 no.2
• /
• pp.113-126
• /
• 1988

Paralytic shellfish poison(PSP) accmulate in shellfish as a result of feeding toxic dinoflagellates. The shellfish do not seem to be harmed by the toxins, but become toxic to humans and other animals that feed on them. The purpose of this study was to investigate the distribution and changes of PSP by species of shellfish, collected area and collected month. Also, the correlation between PSP and toxic dinoflagellate, Protogonyaulax tamarensis, was investigated. Five hundred and six samples of 13 kinds of shellfish for PSP bioassay were collected at the shellfish growing area of Pusan, Masan, Chungmu, $Samch\check{o}npo, Y\check{o}su, Mokpo and Daech\check{o}n$ located in South Korea during the study period from May, 1985 to Octcber, 1987. Most of the samples submitted were free from PSP except sea mussel, short - necked clam and ark shell. Among the intoxicated samples, PSP was most often detected in sea mussel. PSP was detected mainly in spring$(February\~May)$ in the southern coast of Korea. In case of Pusan, exceptionally, toxic sea mussel have been found even June and July in 1987. The toxicity score of toxic shellfishes examined was ranged from 23.44 to $150.26{\mu}g/100g$ of edible meat and toxicity of sea mussel was higher than other toxic shellfishes. By the study of anatomical distribution of PSP in sea mussel collected at Masan in Febuary and March, 1986, the toxin accumulated in digestive gland was about $70\%$ of all. There was no significant correlation between toxicity of sea mussel and cell numbers of P. tamarensis that one of the causitive organism of PSP during the studying period in Masan area. There was almost no difference in toxicity of sea mussel by water depth of collection, but toxicity of surface shellfish was a little higher than those of 3.5, and 7.0m depth.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.17 no.12
• /
• pp.407-414
• /
• 2016

This study was conducted to investigate the effects of supplementation of dietary betaine on nutrient digestibility and physiological responses in finishing pigs. A total of twelve pigs with a body weight of $80.1{\pm}3.7kg$ were individually caged, and randomly assigned to one of the two experimental diets containing 0 (control) or 5 g/kg (treatment) of the betaine in a $2{\times}2$ Latin square design. The experimental period was 14 days-7 days adaptation and 7 days trial period-per phase. All data for the difference between control and treatment groups were statistically analyzed by student's t-test. Dry matter and crude protein digestibility in the treatment group were significantly improved by 1% and 1.3%, respectively, as compared with those in the control (p<0.05). The apparent absorption of dietary energy was increased from 82.3% to 83.7% by dietary betaine supplementation. Thus, the retention of energy was also significantly increased to above 6% in the treatment group compared with the control group (control 4,057 vs treatment 4,314 kcal; p<0.01). The physiological parameters indicating serum biochemical contents and stress-, immune-, and inflammatory- responses were not changed by the supplementation of dietary betaine. In conclusion, dietary betaine improves the nutrient digestibility without any negative effects in terms of physiology in finishing pigs. It suggests that the supplementation of dietary betaine may increase the productivity through the improvement of weight gain and feeding efficiency.

• Korean Journal of Weed Science
• /
• v.5 no.2
• /
• pp.114-120
• /
• 1985

This study was conducted to investigate the important weeds occurring in onion and garlic planted in paddy field as the succeeding crop of rice and to evaluate the effect of competition between these crops and weeds on the yield of onion and garlic. In terms of quantity occurred, Persicaria hydropiper, Chenopodium ficifolium, Alopecurus aequalis, Poligonum aviculare, Echinochloa crus-galli, and Bothrisospermum tenellum appeared to be the major weeds in onion and garlic fields. Annual broadleaves were composed of 90.0 and 94.3% of total weed dry weight in onion and in garlic fields, respectively. Among these weeds, P. hydropiper was the most dominant species in onion and C. ficifolium in garlic fields. The higher Simpson's index such as 0.67 in garlic as compared to 0.40 in onion can be attributed to the presence of a C. ficifolium, which is existed in a single dominant species. The full season competition between the crops and these weeds resulted in yield reduction of onion by 23.9% and garlic by 31.9%, showing weed dry weight, 448.18 and 418.40 g/$m^2$ in onion and garlic fields, respectively. The maintenance of weed free condition for about 4 weeks after weed emergence could be the prerequisite to obtain the maximum yield of these two crops.

• Korean Journal of Weed Science
• /
• v.14 no.4
• /
• pp.265-271
• /
• 1994

This study was conducted to determine the herbicidal activity, persistance, downward movement and effect on bentgrass of 7 dinitroaniline herbicides such as benefin [N-butyl-N-ethyl-2,6-dinitro-4-(trifluoromethyl) benzenamine], beslogine [N,N-dibutyl-2,6-dinitro-4-trifluoromethylaniline], prodiamine [2,4-dinitro-$N^3$,$N^3$-dipropyl-6-(trifluoromethyl)-1,3-benzenediamine], pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine], trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl) benzenamine], ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl) benzenamine], and oryzalin [4-(diprop-ylamino)-3,5-dinitrobenzene-sulfonamide) together with bensulide [O,O-bis(1-methylethyl) S-[2-[(phenyl-sulfonyl) amino]ethyl]phosphorodithioate] and siduron [N-(2-methylcyclohexyl)-N'-phenylurea) as the control. In addition, distribution of pendimethalin and benefin into bentgrass was also determined. Prodiamine, benefin, and pendimethalin at the 1/16 dose of the recommended rate showed very high herbicidal activity($LD_{90}$) on Digitaria sanguinalis, but ethalfluralin and bensulide showed $LD_{90}$ at the 1/4 dose of the recommended rate, showing difference in herbicidal activities among dinitroaniline herbicides. All the herbicides except for pendimethalin had the lower herbicidal activity in sandy soil than that of clay-loam soil. Benefin, beslogine, prodiamine, oryzalin, bensulide and siduron persisted in the soil for about 50 days, but pendimethalin persisted in the soil for about 35 days, and trifluralin and ethalfluralin for about 25 days. Ethalfluralin, oryzalin and bensulide were the most mobile(downward movement) of the 9 herbicides studied. Less mobility was observed in the turfgrass condition than that of the bare soil. Beslogine bensulide prodiamine and benefin had no injury effect on bentgrass(Agrastis atolonifera L., penncross creeping bent grass). However, herbicides like oryzalin, trifluralin and pendimethalin reduced the dry weight of bentgrass by 12%, 30% and 40%, respectively. No significant difference in distribution of pendimethalin and benefin into inner part of leaves, surface and wax layer of bentgrass was observed, and thus it seems that different phytotoxic effect between pendimethalin and benefin may be attributed to different metabolism and mode of action.

• Korean Journal of Soil Science and Fertilizer
• /
• v.35 no.3
• /
• pp.153-161
• /
• 2002

The installation of subsurface drainage equipment is required for generalized use of paddy field and to improve soil productivity. The internal drainage of paddy field has improved root condition from the increasing of oxygen supply and removing noxious elements. This experiment was carried out to determine the effects of fertilization and drainage system on soil characteristic, growth and lodging trait of rice in paddy soil. A subsurface drainage system was installed a depth of 0.8m. Three fertilizer treatments were applied : 1) Conventional fertilized plot, 2) Controlled-release fertilized plot, 3) No-fertilized plot. In conventional plot, 110 kg N (as urea 46%), 45 kg P (as fused phosphate 20%) and 57 kg K (as potassium chloride 60%) per hectare fertilizers were applied. Controlled-release fertilizer was applied by 70% of N compared to the conventional plot. During the rice cropping, the water depth decrease was two times higher in subsurface drainage(SD) plot than non-drained(ND) plot. After harvesting of rice, the bulk density of sub-soil(10-20cm depth) was lower in SD plot than ND plot. After the experiment, the surface soil pH was high at SD plot but sub-soil was high at ND plot. Organic matter content was higher in all soil layer for SD plot than fro ND plot. Available $P_2O_5$ was not different between SD and ND plot for surface soil, but was high for SD plot for sub soil. The $NH_4{^+}-N$ content of soil, shoot dry matter, total nitrogen and $K_2O$ of rice plant were greater after panicle formation stage in SD plot. Total nitrogen content, $P_2O_5$ and $K_2O$ of rice root were high in SD plot after heading. Though the gravity center and 3rd internode length were greater, pulling force of rice root was higher in SD plot than ND plot. Rice yield in SD plot were low at conventional and controlled-release fertilized plot because of the greater field lodging, but yield in SD plot was high at no-fertilized plot. This study indicates that the fertilization level should be decrease on subsurface drainage system for rice cropping.

• Korean Journal of Agricultural Science
• /
• v.20 no.2
• /
• pp.125-132
• /
• 1993

This study was carried out to understand the relationship between sources and sinks in tillering maize hybrid. A non-tillering hybrid was included as check hybrid, The following treatments were imposed on the two hybrids at tasseling stages: 1) no leaves removed, 2) all leaves removed, 3) leaves above 1st ear node removed, 4) leaves below 1st ear node removed and 5) only 1st ear removed. The experiment was arranged in a split plot with hybrids as main plots and treatments as sub plots. Results obtained were summarized as follows: Plant heights in control were significantly higher in both hybrids than other treatments, No significant differences of plant height were observed among treatments of leaf removal in both hybrids. No significant differences of kernel weights of plant were found between locations of leaf removal, But seed weight of tillering hybrids was about 10 to 15% heavier than that of non-tillering hybrid. Stem weight showed the same tendency as the kernel weights. Leaf removal above the ear node lowered kernel weight significantly up to 60% compared with no leaf removal. The leaf removal below the ear node lowered the kernel weight about 40%. The effects of leaf removal on kernel weight was more serious in the non-tillering hybrid than in the tillering hybrid. Contribution of each part to the total dry matter varied with position of leaf removal. When no leaves were removed, contribution of each part to the total dry matter was great in the order of kernel weight, leaf weight and culm weight. When leaves above the ear node were removed, the contribution of each part to the total dry matter was in the order of culm weight, leaf weight and kernel weight. When leaves below the ear node were removed, the contribution of kernel weight was greater than the contribution of leaf and culm weights.

• Asian Journal of Turfgrass Science
• /
• v.25 no.2
• /
• pp.208-216
• /
• 2011

The purpose of this study was to find soil-amendment materials those support the growth of Kentucky bluegrass and reduce salt accumulation at the sand based growing media in saline conditions. Rootzone profile in columns consisted of 20 cm of top soil, 20 cm coarse sand as capillary rise interruption layer and 10 cm reclaimed paddy soil as the base of the profile. Top soils were mixtures of dredged sand (DS) and amendment with compositions of 90% sand + 10% peat moss (SP), 80% sand + 10% soil + 10% bottom ash (SSoBa), 80% sand + 20% soil (SSo), 90% sand + 5% peat + 5% zeolite (SPZ), and 80% sand + 20% bottom ash (SBa). The top soil mixtures of DS and amendments were treated with and without gypsum (Gp). The columns were soaked into 5 cm depth saline water reservoir with the salinity level of $3-5dSm^{-1}$. Irrigation of $2dSm^{-1}$ saline water with rate of $5.7mm\;day^{-1}$ was applied by 3 day interval. Application of zeolite decreased SAR, application of gypsum decreased ECe of the sand amended by peat + zeolite and decreased the SAR of sand amended by bottom ash. The SP and SSoGp resulted in higher clipping dry weight of Kentucky bluegrass. The SSoGp and SPZGp showed longer root lengths. The SP and SBaGp showed higher visual quality. Addition of gypsum to soil and bottom ash treatments resulted in the increased shoot growth, whereas additional gypsum to the treatments of peat, soil and zeolite increased the root growth of Kentucky bluegrass.

• Korean Journal of Plant Resources
• /
• v.27 no.5
• /
• pp.546-555
• /
• 2014

The final aim of this study is to develop a biofiltration system integrated with plant vegetation for improving indoor air quality effectively depending on indoor space and characteristics. However, to approach this final goal, several requirements such as constant pressure drops (PDs) and soil moisture contents (SMCs), which influence the capacity design for a proper ventilation rate of biofiltration system, should be satisfied. Thus, this fundamental experiment was carried out to adjust a proper wind speed and to ensure a stabilization of initial SMCs within biofilter for uniform distribution of SMCs and PDs, and for normal plant growth, especially avoiding root stress by wind. Therefore, we designed horizontal biofliter models and manufactured them, and then calculated the ventilation rate, air residence time, and air-liquid ration based on the biofilter depending on three levels of wind speed (1, 2, and $3cm{\cdot}s^{-1}$). The relative humidity (RH) and PD of the humidified air coming out through the soil within the biofilter, and SMC of the soil and plant growth parameters of lettuce and duffy fern grown within biofilter were measured depending on the three levels of wind speed. As a result of wind speed test, $3{\cdot}sec^{-1}$ was suitable to keep up a proper RH, SMC, and plant growth. Thus, the next experiment was set up to be two levels of initial SMCs (low and high initial SMC, 18.5 and 28.7%) within each biofilter operated and a non-biofiltered control (initial SMC, 29.7%) on the same wind speed ($3cm{\cdot}sec^{-1}$), and measured on the RH and PD of the air coming out through the soil within the biofilter, and SMC of the soil and plant growth parameters of Humata tyermani grown within biofilter. This result was similar to the first results on RHs, SMCs, and PDs keeping up with constant levels, and three SMCs did not show any significant difference on plant growth parameters. However, two biofiltered SMCs enhanced dry weights of the plants slightly than non-biofiltered SMC. Thus, the stability of this biofiler system keeping up major physical factors (SMC and PD) deserved to be adopted for designing an advanced integrated biofilter model in the near future.

• Journal of Plant Biotechnology
• /
• v.41 no.3
• /
• pp.146-158
• /
• 2014

Camelina sativa that belongs to Brassicaceae family is an emerging oilseed crop. Camelina seeds contain approximately 40% storage oils per seed dry weight, which are useful for human and animal diets and industrial applications. Microsomal delta-12 fatty acid desaturase2 (FAD2) catalyzes the conversion of oleic acid to linoleic acid. The polymorphisms of FAD2 genes are correlated with the levels of oleic acids in seed oils. Microsomal delta-12 fatty acid desaturase2 (FAD2) catalyzes the conversion of oleic acid to linoleic acid. The polymorphisms of FAD2 genes are correlated with the levels of oleic acids in seed oils. In this study, three CsFAD2 genes (CsFAD2-1, CsFAD2-2 and CsFAD2-3.1) were isolated from developing seeds of Camelina sativa (L.) cv. CAME. The nucleotide and deduced amino acid sequences of three CsFAD2 genes were compared with those from dicotyledon and monocotyledon plants including Camelina cultivars Sunesone and SRS933. Three histidine motifs (HECGH, HRRHH, and HVAHH) required for FAD activity and a hydrophobic valine or isoleucine residue, which is a SNP (single nucleotide polymorphism) marker related with enzyme activity are well conserved in three CsFAD2s. The expressions of CsFAD2-1 and CsFAD2-3.1 were ubiquitously detected in various Camelina organs, whereas the CsFAD2-2 transcripts were predominantly detected in flowers and developing seeds. The contents of oleic acids decreased, whereas the amounts of linoleic acid increased in dry seeds of transgenic fad2-2 lines expressing each CsFAD2 gene compared with fad2-2 mutant, indicating that three CsFAD2 genes are functionally active. The isolated CsFAD2 genes might be applicable in metabolic engineering of storage oils with high oleic acids in oilseed crops.