• Journal of Life Science
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• v.11 no.6
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• pp.561-567
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• 2001

To isolate and purify fibrinolytic active substance from Sarcodon aspratus(N $H_4$)$_2$S $O_4$ precipitation, DE52 anion exchange column chromatography, Sephacryl-S 200gel filtration chromatography and Mono S cation FPLC were carried out and the characterizations of the purified enzyme were investigated. The bound active fraction on DE52 anion exchange column chromatography were eluted with 0.2 M NaCI and the fibrionlytic enzyme was purified after following Sephacryl-S200 gel fitration chromatography and Mono S cation EPLC. The specific activity of purified enzyme was 55.2 U/mg protein and increased 11.3 fold comparing crude extract and the yield was 49.5%. 12% SDS-PAGE electrophoresis and gel filtration chromatography revealed that Sarcodon aspratus fibrionloytic enzyme was highly purified and had 29.300 Da molecular weight. Enzyme activity of the purified fibrinolytic enzyme from Sarcodon aspratus was increased on higher pH and was stable until pH 10.5. On temperature dependent stability, the enzyme activity was decrease sharply but remained 25% relative activity on 8$0^{\circ}C$. This enzyme activity was inhibited by heavy metal ion, C $U^{2+}$ and $Co^{3+}$ with 68% and 38%, respectively. And also, the enzyme activity was inhibited with $Ca^{2+}$ chelator EDTA and serine protease inhibitor PMSF. These results from this study suggested that the fibrinolycit enzyme from Sarcodon aspratus is a serine protease and the enzyme activity was increased by $Ca^{2+}$ or $Mg^{2+}$ ion.n.ion.n.

• Journal of Life Science
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• v.14 no.1
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• pp.180-187
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• 2004

Crowing evidence indicates that oxidized low density lipoprotein (LDL) nay promote atherogenesis. Therefore, inhibition of LDL oxidation may impede this process. The effect of geraniin on the susceptibility of human low density lipoprotein (LDL) to macrophages-induced oxidation was investigated by monitoring a thiobarbiruric acid reactive substrance (TBARS). The antioxidative activity of geraniin was higher than that of $\alpha$-tocopherol on low density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Geraniin inhibited the C $u^{2+}$ mediated oxidation of human LDL in a dose dependent manner at concentration of 50 and 100 $\mu\textrm{g}$/mL. Geraniin, almost completely inhibited the macrophages mediated LDL oxidation in electrophoretic mobility and conjugate diene of LDL oxidation. Also, geraniin almost completely inhibited 0$_2$$^{[-10]}$ at concentration of 100 $\mu\textrm{g}$/mL. The physiological relevance of the antioxidative activity was validated at the cellular level where geraniin inhibited endothelial cell mediated LDL oxidation, When compound with several other antioxidants geraniin showed a high activity equal to natural antioxidants, $\alpha$-tocopherol and ascorbic acid, and the synthetic antioxidant, protocol. These results indicate that geraniin might play a protective antioxidant effects on LDL, probably affecting both the structural properties of macrophage and endothelial cell for the LDL oxidation..

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.151-156
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• 2003

The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGF$_2$a was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.143-149
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• 2003

This study was carried out to cryopreserve and to investigate characteristics of semen in Hanwoo. Semen was obtained from bulls selected by Daekwanryeong Branch station. Semen was collected each morning of the experiment, placed in water jacketed tubes at 37$^{\circ}C$, and trans-ported to the research laboratory within 10 minutes. Semen was extended with Egg yolk-glycerol extender to contain 50${\times}$10$^{6}$ sperm/ml. Semen was cooled over a 6h period in water jacketed tubes from about 25 to 5$^{\circ}C$, Egg yolk-glycerol extender was added in one step at 5$^{\circ}C$. Semen was aspirated into 0.5ml straws, which were sealed with powder. Egg yolk-glycerol extender, which is used in Hanwoo sperm frozen and stored, semen from 13 Hanwoo bulls collected, the postthawed percentages of motile sperm were 65.7%. In semen characteristics of Hanwoo bulls, number of bulls volume are 5.7 ml and total cell count are 975${\times}$10$^{6}$ m1 ejaculate.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.4
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• pp.314-321
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• 2000

In order to obtain the fundamental data on the auditory thresholds of fishes for catching method using low frequency sound, the auditory thresholds of coralfish Chromis notatus were measured in the presence of masking noise in the spectrum level range of 73~83dB re l$\mu$Pa/√Hz by heartbeat conditioning technique using pure tones coupled with a delayed electric shock. Critical ratios were about 23~41dB at measurement frequency, The critical ratio increased almost linearly with increasing frequency from 500Hz. The noise spectrum level at the start of masking was about 60~65dB. This suggests that hearing of coralfish is masked in the natural environment with the noise spectrum level above 60dB. The sound pressure level of which the signal sound of 300Hz is recognized by coralfish under the ambient noise is above 88dB and the critical ratio of them is above 23dB. The hearing index of coralfish with ambient noise was 81.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.21 no.1
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• pp.12-18
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• 1985

In general the impulse sound sources of underwater generated by electric arc discharge had used static energy of the charged capacitors. The author proposed an underwater arc discharge sound source using secondary voltage of high voltage transformer without capacitors. The arc discharge device was composed of a high voltage transformer and a switching system. The impulse current in the primary turn of the high voltage transformer is controlled by the switching system and inductive current of the secondary turn in the high voltage transformer is used in making impulsive arc discharge. A series of experiment have been carried out to observe the acoustic characteristics of the impulse sound source generated by the arc discharge. The results obtained were as follows: 1. Secondary current at the time of arc discharge keeps after ohm's law in the beginning and the maximum current flows out as soon as arc discharge breaks out. 2. A time difference between a start of applied current and a generation of arc discharge sound is the 3msec and it is generated arc sound when breaking down electric insulation at maximum voltage. 3. The sharper the end of electrodes and the higher the secondary voltage, the higher the sound pressure level. 4. Arc discharge sound was generated even at the distance of 100cm between electrodes and was stably reproductive at the gap of 1cm to 100cm. 5. Electric arc discharge sound wave is a shock wave of pulse-width of 0.15msec and spectral distribution of it is plenty of low frequency components less than 10 KHz.

• The Korean Journal of Food And Nutrition
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• v.18 no.3
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• pp.161-167
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• 2005

This study was conducted to examine the possibility of improvement effect in Takju mash added with ${\alpha}G-Hesperidine$ on the second stage mash. Alcohol was highly generated in comparison with the control on fermentation time in case of the ${\alpha}G-Hesperidine$ on the second stage mash. Reducing sugar was also highly produced after 2 days on the second stage, and amount of reducing sugar was indicated to be decreased between $4\~6$ days. Total acidity was shown not to be practically changed after 2 days in mash added with ${\alpha}G-Hesperidine$ on the second stage in comparison with the control. Fusel oil produced from mash added with ${\alpha}G-Hesperidine$ was less generated in comparison with the control. Yeast growth on the mash added with ${\alpha}G-Hesperidine$ was revealed to be highly in comparison with the control through fermentation periods. Precipitation velocity of suspension in mash added with ${\alpha}G-Hesperidine$ was shown to be 1.5 times lower than that of the control. Precipitation amount in mash added with ${\alpha}G-Hesperidine$ was not nearly changed on the storage time. The astringency and bitterness were slightly decreased, while on the other turbidity and refreshing were increased in mash added with ${\alpha}G-Hesperidine$. In general overall preferences was indicated to be fully satisfied in mash added with ${\alpha}G-Hesperidine$ in comparison with the control. Nasty smell of Takju added with ${\alpha}G-Hesperidine$ was recognized after 6 days during storage.

• Korean Journal of Food Science and Technology
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• v.27 no.2
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• pp.241-245
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• 1995

Focusing on quality problems of delayed chilling porcine muscle, the effects of delayed chilling and aging on the contents of ATP-related compounds and taste of pork were investigated. Twelve Landrace pigs were employed and bisected: left sides were delay-chilled(DC) at room temperature($20^{\circ}C$) for 3 hrs, whereas right sides were conventionally chilled(CC). ATP-related compounds tested were adenosine triphosphate(ATP) and its derivatives in pork muscle, inosine monophosphate(IMP), guanosine monophosphate(GMP) and L-glutamate in cooked broth. DC sides showed more rapid pH decline and degradation of nucleotides than did CC sides. The levels of ATP and adenosine monophosphate(AMP) were not changed significantly. However, adenosine diphosphate(ADP) and IMP showed the highest levels at the 1st and 5th day, respectively. Hypoxanthine(Hx) was gradually increased(p<0.05) during aging. During aging, the IMP contents cooked broth tended to decrease, while the GMP and L-glutamate contents increase. As a result of these, the taste score got better and finally the results of sensory evaluation became increased(p<0.05). However, compared to CC sides, DC sides did not seem to lower taste of pork.

• Fire Science and Engineering
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• v.31 no.6
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• pp.107-111
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• 2017

The purpose of this study was to develop a Eco smoking booth that can effectively reduce hazardous pollutants generated during smoking and evaluate the efficiency and effectiveness of removing hazardous pollutants. The design and manufacture of an eco-friendly automatic smoking booth equipped with deodorizing facilities, such as inlet - HEPA filter - electrostatic precipitator (EP) - impregnated activated carbon - exhaust port, etc., and the efficiency of removing hazardous pollutants from inside and outside was measured and evaluated. The complex odor removal efficiency was 95.37% inside the smoking booth, and 97.38% at the exit of the preventive facility. The carbon monoxide removal efficiency was 94.25% in the inside and 98.32% in the outlet. In addition, the removal efficiency of particulate matter, (PM1, PM2.5, and PM10) inside the smoking booth was 98.59%, and 98.85% at the outlet. The total volatile organic compounds (TVOCs) decreased from $26,000{\mu}g/m^3$ to $5,203{\mu}g/m^3$ in the smoking booth, resulting in 79.99% removal efficiency. After the ventilator was operated, the measured effluent concentration was $5,019{\mu}g/m^3$, and the removal efficiency was 80.70%. Therefore, the smoking booth designed and manufactured through this study can be applied to the removal of harmful pollutants even in the small working environment in the future.

• Korean Journal of Food Science and Technology
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• v.17 no.3
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• pp.213-218
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• 1985

As a way of improving the texture and flavor of soybean cheese, whey-say cheeses were made by coprecipitation of various mixtures of whey, whey powder, soy milk and soy protein powder, and mixed culture of str. lactis, str. cremoris and rennet were added, then the cheeses were cured at $15^{\circ}C$ for up to 10 weeks. Physicochemical characteristics of the cheese were investigated by analyzing pH, titratable acidity(TA), water soluble nitrogen, 10% TCA soluble nitrogen, amino acid composition, beany flavor, color and hardness. The pH of whey-soy cheeses during ripening changed from 5.3 to 4.2 after 5 or 6 weeks and maintained that value while that of soybean cheese maintained a higher pH value. TA of whey-soy milk cheeses was gradually increased to the value of 0.4-0.45 after 8 weeks, but that of soybean cheese reached only 0.2 after the same period. Water soluble and 10% TCA soluble-nitrogen increased steadily during ripening. Hardness of the whey-soy milk cheeses reached maximum after three weeks of ripening and greatest at those made from 3 : 1 mixture of whey and soy milk and that from soymilk. Color of the whey-soy milk chesses was lighter than that of soybean cheese. The bean flavor of soybean cheese was strong and persistent for the whole ripening period. Acid flavor was dominant in the whey-so milk cheese and masked the beany flavor partially.