The followings are the results obtained in a series of experiments concerning of the varieties, the short-day-treatment, the fertilizer application, and the planting space of soybean, which were carried out to investigate the methods best suited for cultivating green soybean as the preceding crop of the rice. in the paddy-field in the middle parts of Korea at the practice farm attached to Agriculture College of Kon Kuk University in 1972. 1. Though the varieties of soybean was planted on the hot-bed on March 15 and then set in the main plot, none of them did flower within May 15, which is the limit time of flowering in growing soybean as the preceding crop of the rice in the paddy-field without the short-day-treatment being applied during raising seedlings. 2. The earliest-maturing variety groups such as HOKKAl#l, WASEMIDORI, YAEHUSANARI, MIT AKARAHAKUCHO, and VERDE flowered within May 15 by the short-day-treatment during raising seedlings. 3. The optimum hours of the day length was known to be 7 to 9 in the medium-maturing and late-maturing variety groups and 7 to 11 in the early-maturing and the earliest-maturing variety groups in the case of appling the short-day-treatment for 10 days from the beginning of the primary regular compound leaf development. 4 The optimum days in appling the short-day-treatment for 11 hours a day was recognized to be about 10 days regardless of the maturity of varieties. 5. Reduction of days required to flower by the short-clay-treatment, that is, light-sensitivity was remarkably higher in the medium-maturing and the late-maturing variety groups than in the earliest-maturing and the early-maturing variety groups. 6. The yield showed an increase of about 17 per cent in the case of appling the standard amount of nitrogen(4.0 kg/10 a), but it tended to reduce on the contrary in appling the increased amount of nitrogen. 7. The application of increased amount of phosphate had less significant effect on the yield increase than in the case of application of its standard amount( 4.0 kg/l0 a). 8. When the number of transplantation plant was changed from 54 to 130 per 3.3 $m^2$, the yield in 130 plant plot was about two times so higher than in 54 plant plot that the effect of close planting cultivation on the yield was proved to be remarkable. 9. Conditions possible for cultivating green soybean as the preceding crop of the rice in the paddy-field in the middle parts of Korea are turned to be as follows: (a) to plant the earliest-maturing-variety groups on the hot-bed on March 15. (b) to apply the short-day-treatment by 11 hours a day for 16 days from April 16, which is about the time when the primary regular leaf begin to develop. And it was, found to be a most remarkable in the increased yield that apply nitrogen 4.0 kg, phosphate 4.0 kg, and potassium 6.0 kg per 10 a as basic manuring totally and apply the close planting by 130 plants per $3.3{m^2}$(50 cm $\times$ 5 cm).
The studies reported herein were conducted to investigate the effect of thermal conditions in double-cropping of rice. The accumulated daily mean and minimum air temperatures, for the period of the last 30 years, were examined at the 10 different meteorogical stations which are located in the southern part of Korea. The results obtained could be summarized as follows: 1. The first cropping. a. It seemed to be free from any frost-damage of rice at the seeding stage at Yeosu, Pusan and Cheju. However, it was found that there were some dangers of frost damage for about 30 to 40 day at Iri, Chonju and Kwangju, for 18 to 28 days at Daeku and Ulsan, and for 4 to 14 days at Mokpo and Pohang, respectively. b. The early critical transplanting date seemed to be from middle to late-April in the first cropping. As compared with the ordinary lowland seedlings, the semi-protected and upland ones could be planted 5 and 10 days earlier, respectively. c. The early critical heading date was about late-June and there were some low-temperature damages for 8 to 25 days at young-ear formation stage of rice plant, depending upon location. d. The early critical ripening date (the early critical transplanting date of the 2nd cropping) was from late-July to early-August. It took about 32 to 39 days in ripening. There was a tendency of SS${\fallingdotseq}$SL$15^{\circ}C$ (${\theta}$15) and the minimum of $10^{\circ}C$ (${\theta}$10), the ten locations could be devided into two ripening groups of ${\theta}$15>${\theta}$10 and ${\theta}$15<${\theta}$10. c. The late critical ripening date was around October 9 at Iri, Chonju, Kwangju and Daeku and around October 28 at Mokpo, Yeosu, Pusan and eheju. Three to four days were more required for a complete ripening of rice, as compared with the above dates. d. There was an overlap of about 12 to 42 days between the first and second cropping when early-maturing varieties requiring an accumulated mean air temperature of $1, 550^{\circ}C$, from transplanting to heading, were grown. Therefore, some varieties which could head with an accumulated daily mean air temperature of 1, 000 to $1, 200^{\circ}C$, should be either developed or some new cultural technology be established in order to have a successful double cropping in rice.
Fresh edible sweet corns demand relatively short period to harvest fresh ears, which can allow farmers to make a choice sweet corns for various cropping systems. For this reason, we were to find the optimum planting date of late-planted sweet corns to sell fresh ears in the autumn linked to cropping system with winter crops, investigating yield and properties of marketable fresh ears and growth traits of sweet corns (cv. 'Godangok' and cv. 'Guseulok') depending on planting dates such as 10 July, 20 July, and 30 July in Suwon 2012 and 2013, respectively. The 20 July-planted sweet corns showed the most fresh ear yield. However, the 10 July-planted and the 30 July-planted had 32% less yield caused by consecutive rainfall from 10 July through 20 July, and 15% less yield due to low air temperature during ripening than the 20 July-planted, respectively. The 10 and 20 July-planted sweet corns had average 140g of a fresh ear weight and 15% heavier ear than the 30 July-planted. For the July-planted sweet corns, silking days after planting ($r=-0.80^{**}$), and harvesting days after silking ($r=-0.97^{**}$) and planting ($r=-0.91^{**}$) were highly negatively correlated with daily mean air temperature during the period, resulting in it takes 1,100 growing degree days (GDD) to harvest fresh ears from the July-planted sweet corns. The fresh ears of the 20 July-planted sweet corns are able to be harvested by early October. Therefore it will be a good choice for the cropping system based on winter vegetable cash crops such as temperate garlic and onion with medium or late maturity. Among three planting dates 20 July-planted sweet corns had the best field performance in every year considering fresh ear yield, ear size, and stability to grow.
This study was conducted for ten years to evaluate the effective soil management for preventing the infection of root-knot nematode in the field of continuous cultivation with oriental melon under plastic house in Songju area of kyongbuk province. The content of available phosphate, total nitrogen, organic matter, CEC, and exchangeable base in the soil increased with the increase of continuous cultivation year. Especially salt content in the soil increased form 1.2 to 4.55 mS/cm and the yield of oriental melon dramatically decreased with the continuous cultivation year. The number of root-knot nematode was 91 per $300\;cm^3$ of soil in the field of continuous cultivation for 3 years and showed slight damage on the oriental melon, but it was 518 in the field of $4{\sim}6$ years continuous cultivation and showed that 50% of plants died in August, and the yield of late season was less than 50% compared to normal plant. For the seasonal changes in infection rate of root-knot nematode on oriental melon plant, 15% of the normal plant was infected by nematode in February and increased gradually by $10{\sim}20%$ per month, 60% of plants was infected in July. The density of root-knot nematode nymph was 167 in February and increased to 1,625 in August. The infection rate of nematode was 35%, and the number of nematode was about 54 in nursery soil originated from paddy soil, upland soil, and river sand. There were no relationship between the number of nematode and available phosphate or exchangeable base in the soil of plastichouse where oriental melon plants were grown.
This study was performed to investigate the correlations between steroids and TGF-${\beta}_1$ levels and delayed parturition in SCNT clone calving. The recipients pregnant by AI were used as control (AI-R). All AI-R were labored by natural delivery (n=5, day $284{\pm}0.71$ of pregnancy). The recipients pregnant by SCNT embryo (SCNT-R) showing no signs of delivery about 10 days after expected date were operated by Caesarean section (n=5, day 292). The blood and placentome samples were obtained and weighed at parturition. The concentrations of plasma progesterone (P4) and Estradiol-$17{\beta}$ (E2) were measured by radioimmunoassay (RIA). The levels of plasma and placental TGF-${\beta}_1$ levels were examined by ELISA. The placentomes from SCNT-R were overweight (p<0.05) compared to those of AI-R. The plasma P4 (p<0.01) level in SCNT-R at parturition was significantly higher compared to that of AI-R. In contrast, the plasma E2 level in the SCNT-R was significantly lower compared to that of AI-R (p<0.05). The plasma and placental TGF-${\beta}_1$ protein levels in the SCNT-R were significantly higher than those of AI-R at parturition, respectively (p<0.01). Based on these results, aberrant expressions of steroid hormones and high levels of plasma and placental TGF-${\beta}_1$ protein at parturition may be one of the key indicators on delayed parturition of SCNT clone calving.
Purpose: The purpose of this study was to investigate the consumption behaviors of sugar-sweetened beverages and blood lipid profiles according to food-related lifestyles of adults in Incheon. Methods: Subjects were recruited from 19 to 64 year old adults residing in Incheon who visited for the purpose of a health examination at D hospital. A total of 110 subjects consented to participate in this study and to allow their health examination records to be used at D hospital. Data were collected using self-administered questionnaires and anthropometric data, and blood lipid profiles were collected from health examination records. Statistical analysis was performed using the SPSS 20.0 program. Results: In the factor analysis, food-related lifestyles were categorized into four factors: planned purchase seeking, taste seeking, well-being seeking, and convenience seeking. Using the K-average clustering method, food-related lifestyles of subjects were categorized into two groups: health and rationality-oriented group (HRG) and convenience purchase-oriented group (CPG). Average total scores of sugar-related nutrition knowledge in the HRG were significantly higher compared to the CPG, and average scores of consumption habits regarding sugar-containing foods in the HRG were significantly lower compared to the HRG. When subjects chose sugar-sweetened beverages, the CPG showed a significantly higher frequency of checking food labeling and considering nutrition compared to the CPG. Drinking frequency of carbonated beverages in the CPG was significantly higher compared to the HRG. Conclusion: There were significant differences in sugar-related nutrition knowledge and consumption habits of sugar-sweetened beverages between the two food-related lifestyle groups. These results could be useful for establishing guidelines for reducing consumption of sugar-sweetened beverages. In addition, it is necessary to continue sugar-related nutrition education by considering the food-related lifestyles of adults.
Intracellular calcium concentration ($[Ca^{2+}]_i$) may play a crucial role in a variety of neuronal functions. Here we report that in primary culture of mouse cerebellar granule cells nicotinic acetylcholine receptors (nAChRs) are expressed in a specific developmental stage and involved in the regulation of intracellular calcium homeostasis. Nicotine-mediated calcium responses were measured using $^{45}Ca^{2+}$ or fluorometrically using the calcium-sensitive fluorescent dye fura-2. Maximal uptake of $^{45}Ca^{2+}$ evoked by nicotine in mouse cerebellar granule cells were revealed $8{\sim}12$ days in culture. In contrast, nicotine did not alter the basal $^{45}Ca^{2+}$ uptake in cultured glial cells. In cerebellar granule cells nicotine-evoked $^{45}Ca^{2+}$ uptake was largely blocked by the NMDA receptor antagonists. Glutamate pyruvate transaminase (GPT). which removes endogenous glutamate, also prevented nicotine effects, implying the indirect involvement of glutamate in nicotine-mediated calcium responses. Fluorometric studies using fura-2 showed two phases of nicotine-evoked $[Ca^{2+}]_i$ rises: the initial rising phase and the later plateau phase. Interestingly, the NMDA receptor antagonists and GPT appeared to inhibit only the later plateau phase of nicotine-evoked $[Ca^{2+}]_i$ rises. The present results imply that nicotine mediated $^{45}Ca^{2+}$ uptake and $[Ca^{2+}]_i$ rises are attributed to the calcium fluxes through both nAchRs and NMDA receptors in a time-dependent manner. Consequently, nAChRs may play an important role in neuronal development by being expressed in a specific developmental stage and regulating the intracellular calcium homeostasis.
In order to elucidate the behavior of bensulfuron-methyl, a sulfonylurea herbicide, in a soil/plant microecosystem, rice plants (Oryza sativa L.) were grown for 12 weeks in the specially made stainless steel pots (17cm I.D. $\times$ 10cm H.) containing two different paddy soils treated with fresh and 13-week-aged residues of [phenyl-$^{14}C$]bensulfuron-methyl, respectively. During the aging period, the mineralization to $^{14}CO_2$ from soil A (OM, 3.59%; CEC, 7.65 $cmol^+\;kg^{-1}$; texture, sandy clay loam) and B (OM, 1.62%; CEC, 4.51 $cmol^+\;kg^{-1}$; texture, sandy loam) amounted to 6.79 and 10.15% of the originally applied $[^{14}C]$bensulfuron-methyl, respectively. The amounts of $^{14}CO_2$ evolved from the soils with fresh residues were higher than those from the soils with aged residues. At harvest after 12-week growing, $^{14}C$-radioactivity absorbed and translocated into rice plants from soils A and B containing fresh residues of bensulfuron-methyl was 1.53 and 4.40%, while 4.04 and 6.37% in the two soils containing aged residues, respectively. Irrespective of aging and soil type, the $^{14}C$-radioactivity remaining in soil ranged from 80.41 to 98.87% of the originally applied $[^{14}C]$bensulfuron-methyl. The solvent extractability of tile soils was $39.25\sim70.39%$, showing the big differences among the treatments. Most of the nonextractable soil-bound residues of $[^{14}C]$bensulfuron-methyl were incorporated into the fulvic acid fraction$(61.32\sim76.45%)$. Comparing the microbial activity of the soils with rice plants grown with that of the soils without them, the former was $1.6\sim3.0$ times higher than the latter. However, it did not correlate with the $^{14}CO_2$ evolution.
Kim, A-Hyeong;Yeon, Cho-Long;Kim, Joo-Hyung;Kim, Heung-Tae
The Korean Journal of Pesticide Science
/
v.16
no.2
/
pp.171-177
/
2012
Pepper seedlings of 6-leaf stage were inoculated with cell suspension of Xanthomonas euvesicatoria 1523 7 days after the application of Bion-M by soil-drenching. Disease severity in the treatment with 20 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, with which Bion-M was composed, was 19%, whereas that of the untreated control was 75%. The resulting control value of acibenzolar-S-methyl was calaulated as much as 74.7%. The control value of acibenzolar-S-methyl was dependent with the applied concentrations and ranged 29.3% to 49.3% at 4.0 and 0.8 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, respectively. Phytotoxicity was observed at 20.0 ${\mu}g\;mL^{-1}$, as lower leaves became to be yellowed and defoliated. The cell density of inoculum suspension of X. euvesicatoria 1523 affected the control value of acibenzolar-S-methyl. With optical density (O.D.) of 0.5 the control value of 4.0 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl was 86.0%. However, the control value improved as high as 97.8% at the O.D. value of 0.1. The control value was 75.0% in adult plant of pepper, when acibenzolar-S-methyl was treated by soil-drenching 7 days before inoculation with cell suspension of X. euvesicatoria 1523. The control effect of acibenzolar-S-methyl on pepper bacterial spot was obtained in pepper field, showing that the control value at 10.0 ${\mu}g\;mL^{-1}$ was 71.2%.
Fenoxycarb, pyriproxyfen and methoprene are juvenile hormone mimic insecticide. These insecticides have been widely used for mosquito, fly, scale insects, and Lepidoptera. The purpose of this study was to develop a simultaneous determination procedure of fenoxycarb, pyriproxyfen and methoprene residues in crops using HPLC-UVD/MS. These insecticide residues were extracted with acetone from representative samples of four raw products which comprised brown rice, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and then n-hexane/dichloromethane partition was followed to recover these insecticides from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The analytes were quantitated by HPLC-UVD/MS, using a $C_{18}$ column. The crops were fortified with each insecticide at 3 levels per crop. Mean recovery ratios were ranged from 80.0 to 104.3% in four representative agricultural commodities. The coefficients of variation were less than 4.8%. Quantitative limit of fenoxycarb, pyriproxyfen, and methoprene was 0.04 mg/kg in crop samples. A HPLC-UVD/MS with selected-ion monitoring was also provided to confirm the suspected residues. The proposed simultaneous analysis method was reproducible and sensitive enough to determine the residues of fenoxycarb, pyriproxyfen and methoprene in the agricultural commodities.
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