• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.2
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• pp.163-168
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• 1984

The composition of amino acids and the related compounds of nucleotides in the dorsal muscle of sweet fish Hemibarbus labeo, Mandarin fish Siniperca scherzeri and read fish Gobius similis was analyzed by amino acid autoanalyzer and high performance liquid chromatography. The result showed that IMP was dominant in the nucleotides of all the dorsal muscle of the fishes and a less amount of UMP, hypoxanthine and AMP was detected. In the free amino acid composition, the important amino acids were taurine and histidine in sweet fish, taurine, glycine and histidine in cornet fish, taurine, histidine and alanine in mandarin fish, taurine, proline and threonine in read fish, respectively, and in all the dorsal muscle of fishes, taurine was the dominant amino acid. In the amino acid compositions of the muscle protein, glutamic acid, glycine, aspartic acid and lysine were reached to 44.0% of total amino acids in sweet fish, glutamic acid, aspartic acid, lysine and glycine were 43.5% in cornet fish, glutamic acid, aspartic acid, lysine and leucine were 43.3%, 43.5% of total amino acids in mandarin fish and read fish, respectively. Glutamic acid was the dominant amino acid in all the fresh fishes.

• Journal of the Society of Naval Architects of Korea
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• v.32 no.2
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• pp.93-102
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• 1995

A series of test was performed by measuring the failure strength and the failure mode of fiber reinforced composite laminates joint containing two holes in Series or Parallel. $[0^{\circ}/45^{\circ}/90^{\circ}/-45^{\circ}]_s$ laminate with W/d(Side distance ratio) 4.0 and E/d(Edge distance ratio) 3.0 has the full bearing strength and are preferable in case of the good efficiency in two series hole. Comparisons were made between testing results and predicting values of the FEM model. Good agreements were fecund between them except the case of $E/d=2{\sim}3$. In the case of $G_h{\geq}3.0d$ and $G_v{\geq}3.0d$ since the interaction coefficients between two parallel holes and between two series holes were small, holes can be treated as independent. The Acoustic Emission(AE) and SEM method were utilized to find out the initial defects, damage and the fracture mechanism.

• Child Health Nursing Research
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• v.20 no.3
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• pp.159-167
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• 2014

Purpose: The purpose of this study was to investigate factors associated with sexual debut among Korean middle school students. Methods: From the database of the Eighth Korea Youth Risk Behavior Web-based Survey (KYRBS), the researchers selected 37,297 middle school students aged 12-15 years. Data were analyzed using $x^2$ test and multiple logistic regression with the SPSS WIN 18.0 program. Results: The proportion of middle school students who had a sexual debut was 2.1% of boys and 1.5% of girls. In multiple regression analysis, economic status, experience of part time work, smoking, drug use and depression were associated with an increased proportion of sexual intercourse for both boys and girls. Living with parents, drinking alcohol and suicidal ideation were associated with sexual debut for boys only. By contrast, formal sexual education was associated with a reduced risk of sexual debut. Conclusion: The results of this study show the factors associated with sexual debut among Korean middle school students. Gender-related interventions should be taken into consideration in school sexual education programmes, because of differences in related factors according to gender.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.81-86
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• 2008

This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M $BH_3$ medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.341-344
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• 1997

Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.759-763
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• 1993

The antioxidant effects of aqueous green tea extract(AGTE) obtained from green tea and its combinations with several synergists on lard were investigated to equalize with that of crude catechin in green tea. The antioxidant effect of crude catechin was about 3-fold higher than that of AGTE. The suitable synergists for AGTE were ${\delta}-tocopherol$ and lecithin, while the tendency of their synergistic effects was different from each other. Addition of ${\delta}-tocopherol$ within 0.05% dosage to lard showed significant synergistic effect, and its synergistic effect decreased with the concentration of AGTE over 0.1% dosage. In combination of AGTE and lecithin, they gradually enhanced synergistic effect on lard with increasing the concentration. The combinations of AGTE, ${\delta}-tocopherol$ and lecithin showed intense synergistic effects and the optimum level of the each component was 0.1% AGTE, 0.05% ${\delta}-tocopherol$ and 1% lecithin. By adding the optimum level of three components to lard, the induction period was lenghtened approximately 8-fold than that of the control owing to their synergism. Comparing the antioxidant effect between the AGTE with synergists and crude catechin, the former was 60% and 20% higher than the latter at 0.1% and 1% dosage respectively.

• Journal of Korean Society of Transportation
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• v.18 no.5
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• pp.17-29
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• 2000

본 연구는 지하횡단보도 이용시 계단에서 나타나는 보행자 특성에 관한 연구로써, 교차로에서 횡단보도와 지하보도에서 보행자의 편의성을 정량화한 것이다. 지하보도 계단에서의 보행자특성에 관한 연구, 즉 속도, 밀도와 보행자교통량에 관한 연구는 비디오촬영을 통하여 수집한 자료를 분석하였다. 지하보도나 횡단보도 횡단시 이동 거리, 소요시간, 소요에너지는 보행자특성 분석에서 나온 결과와 직접 시설을 조사한 자료를 이용하여 비교하였다. 주요 연구결과는 다음과 같다. 첫째, 지하보도 계단에서 보행자 속도는 상향이동시의 평균속도는 37.7m/분(또는 0.67m/초)이고, 하향이동시의 평균속도는 46.7m/분(또는 0.77m/초)으로 나타났다. 둘째, 평균 이동거리는 단순횡단의 경우 지하보도는 119m이고, 횡단보도는 78m이다. 대각횡단의 경우 지하 보도는 161m이고, 횡단보도는 111m이다. 평균 소요시간은 단순횡단의 경우 지하보도는 125.6초이고, 횡단보도는 111.3초로 나타났다. 대각횡단의 경우 지하보도는 162.3초이고, 횡단보도는 178.8초로 나타났다. 평균 소요에너지는 단순횡단의 경우 지하보도는 20.2kcal이고, 횡단보도는 4.7kcal이다. 대각횡단의 경우 지하보도는 23.5kcal이고, 횡단보도는 6.6kcal이다. 단순횡단시 지하보도가 횡단보도보다 이동거리는 평균 1.5배 더 길고, 시간은 평균 1.2배 더 소요되고, 에너지는 평균 4.5배가 더 소요된다. 대각횡단시 이동거리는 평균 1.5배 더 길고, 소요시간은 비슷하고, 에너지는 평균 3.5배 더 소요되었다. 본 연구는 기존의 교차로나 가로구간에 지하보도만 설치되어 있는 지점에 횡단보도의 설치여부에 관한 정책결정을 하는데 도움이 될 수 있을 것이다.

• Journal of Embryo Transfer
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• v.29 no.1
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• pp.73-81
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• 2014

The in vitro production of porcine embryos was essential to increase of blastocyst development rate and select of high quality blastocyst in early stage. There were a lot of reports about in vitro porcine embryo development, but there was no report about the selection of high quality embryos. Therefore, in this study, we investigated the effect of vitamin $K_1$ (vit $K_1$) on the development and survival rate of porcine in vitro fertilized embryos. When vit $K_1$ was treated for 24 hr at day 1 in vitro culture, blastocyst development rate in the control group ($35.5{\pm}3.2%$) was significantly lower compared to $1.0{\mu}M$, $3.0{\mu}M$, or $6.0{\mu}M$ groups ($14.5{\pm}4.3$, 0.0, or 0.0%; p<0.05). The survival rates of blastocysts at day 8 in $1.0{\mu}M$, $3.0{\mu}M$ or $6.0{\mu}M$ of vit $K_1$ treated groups ($22.2{\pm}2.9$, 0.0 or 0.0%) were significantly lower than that of the control group ($31.8{\pm}2.6%$; p<0.05). We were added at $1.0{\mu}M$, $3.0{\mu}M$ or $6.0{\mu}M$ vit $K_1$ for different durations of time at day 1 in vitro culture. The development rate and survival rate in the group of $1.0{\mu}M$ vit $K_1$ for 6 hr was $26.5{\pm}2.9%$ and $47.2{\pm}2.8%$, respectively, which were differed significantly in the group of 12 hr (p<0.05). In the group of $3.0{\mu}M$ vit $K_1$, the blastocyst development in control group was $36.4{\pm}3.1%$ but, the survival rate $41.7{\pm}3.2%$ in the group of 3.0 hr was significantly higher than that of the control group (p<0.05). In the group of $6.0{\mu}M$ vit $K_1$, the control group's the blastocyst development was $32.0{\pm}2.8%$ and the 0.5 hr supplement group's survival rates was $42.9{\pm}1.8%$ higher than other groups. We added vit $K_1$ at day 1, day 2, day 4 and day 6 of in vitro culture, on the based the results of supplemented concentration and duration. In the group of $1.0{\mu}M$ 6.0 hr addition, the blastocyst development rate of day 4 and the survival rate of day 2 were the highest in each group. In the groups of $3.0{\mu}M$ 3.0 hr addition or $6.0{\mu}M$ 0.5 hr addition, the blastocyst development ($59.5{\pm}4.1%$ and $50.0{\pm}3.6%$) and survival rates ($72.7{\pm}5.4%$ and $79.2{\pm}4.0%$) on day 4 were significantly higher than that of control and other experiment groups (p<0.05). Meanwhile, the number of cells in blastocysts that produced by vit $K_1$ supplementation was $53.4{\pm}5.8$, $49.4{\pm}3.8$ and $51.5{\pm}4.5$ respectively, which were significantly higher than that of $40.2{\pm}2.3$ in the control group (p<0.05). There was no difference of the number of apoptotic cells between control and experiment groups. In addition, gene expression of survival blastocyst, the Bax mRNA expression was similar between the control and the experiment groups. However, Bcl-xL mRNA expression's in the group of $6.0{\mu}M$ 0.5 hr on day 4 was highest among control and experiment groups (p<0.05). In this study suggested that the control of concentration, duration and time was effective on the survival and cell number of porcine blastocyst derived from in vitro. We are not know what the exact reasons of the effect of vit $K_1$ on embryo development and need to fur ther study. However, vit $K_1$ might be using the selection of high quality porcine blastocyst.

• Korean Journal of Environmental Agriculture
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• v.14 no.3
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• pp.319-328
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• 1995

This study was carried out to investigate the resistance mechanism by three different kinds of procymidone-resistant and susceptible isolates of Botrytis cinerea which had been collected from green houses. The average resistance level of the resistant strains was 1,000 times higher than that of susceptible ones. Also, it was revealed that the resistance was not originated from components excreted by Botrytis cinerea, based on the result obtained from the treatment with piperonyl butoxide and triphenyl phosphate as an inhibitor of monooxygenase and esterase, respectively. The total lipod content of resistant strains was 1.3 times higher than that of susceptible ones, among fatty acids, palmitic acid, stearic acid, and linoleic and being 3.0, 2.5, and 2.0 times higher, respectinely. Also slight differences in sterol contents and components were observed. The crude chitin content was slightly higher in susceptible strains but contents of N-acetyl glucosamine, a hydrolysate of chitin, were about 2 times higher in resistant ones.

• Membrane Journal
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• v.11 no.4
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• pp.143-151
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• 2001

We have fabricated mixed-ionic conducting membranes, L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ and L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ by the solid state method. Ceramic membranes consisted of perovskite-type structures and exhibited high relative density, >95%. Especially, dense L $a_{0.6}$S $r_{0.4}$Co $O_{3-}$$\delta$/ layer was coated on the L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes by using screen printing technique in order to improve oxygen ion flux. We measured oxygen ion flux on uncoated L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/, uncoated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/, and coated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes. The L $a_{0.6}$S $r_{0.4}$ $Co_{0.2}$F $e_{0.8}$ $O_{3-}$$\delta$/ membranes showed the highest flux, 0.26 mL/min.$\textrm{cm}^2$ at 90$0^{\circ}C$, after steady state had been reached. The oxygen flux of coated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes showed higher value, 0.19 mL/min.$\textrm{cm}^2$ at 95$0^{\circ}C$. This flux was as much as 2 or 3 times higher than those of uncoated L $a_{0.7}$S $r_{0.3}$G $a_{0.6}$F $e_{0.4}$ $O_{3-}$$\delta$/ membranes. 3-$\delta$/ membranes.X> 3-$\delta$/ membranes.membranes.