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Characterization of a cDNA Encoding Transmembrane Protein 258 from a Two-spotted Cricket Gryllus bimaculatus (쌍별귀뚜라미(Gryllus bimaculatus)의 GbTmem258 cDNA 클로닝과 발현분석)

  • Kisang Kwon;Honggeun Kim;Hyewon Park;O-Yu Kwon
    • Journal of Life Science
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    • v.33 no.10
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    • pp.828-834
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    • 2023
  • The cDNA that encodes transmembrane protein 258 (Tmem258) was cloned from Gryllus bimaculatus and named GbTmem258. This protein comprises 80 amino acids, has no N-glycosylation site, and contains five potential phosphorylation sites at two serines, two threonines, and one tyrosine. The predicted molecular mass of GbTmem258 is 9.06 kDa, and its theoretical isoelectric point is 5.5. The tertiary structure of GbTmem258 was predicted using the available secondary structure information, which suggests the presence of alpha helices (52.5%), random coils (22.5%), extended strands (16.25%), and beta turns (8.75%). Homology analysis revealed that GbTmem258 exhibits high similarity at the amino-acid level to Tmem258 found in other species. The effect of starvation and refeeding on GbTmem258 mRNA expression was also examined in this study. It was found that GbTmem258 mRNA expression in the hindgut progressively increased throughout the starvation period, peaking at almost 1.5 times the control level after six days of starvation. However, refeeding for one to two days after the six-day starvation period restored GbTmem258 mRNA expression to the control level. In fat body, GbTmem258 mRNA expression was almost 3-fold higher during starvation compared to the control level. Refeeding for one to two days after the six-day fast resulted in a decline in the expression to about a 2.5-fold increase over the control level. Throughout the starving and refeeding periods, no other tissues showed any discernible alterations in GbTmem258 mRNA expression.

'Inventing' Religion and Pseudo-religion in the 2022 National Curriculum on Religions (2022년 종교 교육과정 - 종교인 만들기와 '유사종교' 발명 교육 -)

  • Ko Byoung-chul
    • Journal of the Daesoon Academy of Sciences
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    • v.46
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    • pp.1-32
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    • 2023
  • The purpose of this article is to critically reflect on the 2022 national curriculum on religions. The perspective of this reflection is that since the religious curriculum is meant to be a national curriculum, it should be applicable to all high school students, be shareable, and function as a place for meta-reflection regarding the proper use of the category of religion. For this purpose, I reviewed the form and content of the 2022 curriculum on religions in Section 2. The form of the 2022 curriculum on religions looks similar to the previously utilized curriculum. However, the main change is that the subject of religions was arbitrarily placed into the category of 'subjects for choosing a career.' And the 2022 curriculum on religions has two characteristics in terms of content: the orientation of 'making religious people (spiritual formation)' and the reemergence of the concept of 'pseudo-religion.' In Section 3, I delved into the orientation of 'making religious people through religious reflection' among the characteristics of the 2022 curriculum on religions. In this process, I discovered that the concept of 'reflection as a metacognitive technology,' which was the core of the prior curriculum and school education, was transformed into the concept of 'religious reflection,' and the concepts of spirituality and religiosity were also added. In Section 4, I delved into the dichotomy of 'religion and pseudo-religion.' 'Pseudo-religion' is a new focus in the 2022 curriculum on religions. In this process, I revealed that the concept of 'pseudo-religion' is a combination of an outdated administrative term of the Japanese Government-General of Korea during Japan's occupation of Korea, and as such, the term is inherently value-laden and harmful. I also revealed that determining 'pseudo-religion' in school education regenerates the colonial Japanese Government-General's biased attitudes toward Korean religions and forces teachers to 'invent' (detect or personally appraise) modern day pseudo-religions through arbitrary judgements. The 'curriculum to emphasize religious reflection and detect pseudo-religions in order to create religious people' can distort the subject of religion in the national curriculum as into a 'subject for religion (promotion or degradation).' If this distortion continues, the appropriateness of curriculum on religions existing within the national curriculum will eventually become a subject of debate.

Data-centric XAI-driven Data Imputation of Molecular Structure and QSAR Model for Toxicity Prediction of 3D Printing Chemicals (3D 프린팅 소재 화학물질의 독성 예측을 위한 Data-centric XAI 기반 분자 구조 Data Imputation과 QSAR 모델 개발)

  • ChanHyeok Jeong;SangYoun Kim;SungKu Heo;Shahzeb Tariq;MinHyeok Shin;ChangKyoo Yoo
    • Korean Chemical Engineering Research
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    • v.61 no.4
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    • pp.523-541
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    • 2023
  • As accessibility to 3D printers increases, there is a growing frequency of exposure to chemicals associated with 3D printing. However, research on the toxicity and harmfulness of chemicals generated by 3D printing is insufficient, and the performance of toxicity prediction using in silico techniques is limited due to missing molecular structure data. In this study, quantitative structure-activity relationship (QSAR) model based on data-centric AI approach was developed to predict the toxicity of new 3D printing materials by imputing missing values in molecular descriptors. First, MissForest algorithm was utilized to impute missing values in molecular descriptors of hazardous 3D printing materials. Then, based on four different machine learning models (decision tree, random forest, XGBoost, SVM), a machine learning (ML)-based QSAR model was developed to predict the bioconcentration factor (Log BCF), octanol-air partition coefficient (Log Koa), and partition coefficient (Log P). Furthermore, the reliability of the data-centric QSAR model was validated through the Tree-SHAP (SHapley Additive exPlanations) method, which is one of explainable artificial intelligence (XAI) techniques. The proposed imputation method based on the MissForest enlarged approximately 2.5 times more molecular structure data compared to the existing data. Based on the imputed dataset of molecular descriptor, the developed data-centric QSAR model achieved approximately 73%, 76% and 92% of prediction performance for Log BCF, Log Koa, and Log P, respectively. Lastly, Tree-SHAP analysis demonstrated that the data-centric-based QSAR model achieved high prediction performance for toxicity information by identifying key molecular descriptors highly correlated with toxicity indices. Therefore, the proposed QSAR model based on the data-centric XAI approach can be extended to predict the toxicity of potential pollutants in emerging printing chemicals, chemical process, semiconductor or display process.

Comparison of the Growth Performance of 12 Crossbred Korean Native Chickens and Commercial Layer from Hatch to 16 Weeks (12개의 토종닭 교배조합과 실용 산란계의 육성기 성장능력 비교)

  • Eunsoo Seo;Myunghwan Yu;Elijah Ogola Oketch;Shan Randima Nawarathne;Nuwan Chamara Chathuranga;Bernadette Gerpacio Sta. Cruz;Venuste Maniraguha;Jun Seon Hong;Doo Ho Lee;Minjun Kim;Jung Min Heo
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.303-310
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    • 2023
  • The current study was conducted to compare the effect of crossbred on the body weight and laying performance of Korean native chicken from hatch to week 40. A total of 873 one-day-old chicks were divided into twelve crossbreds (i.e., CFCK, CFYC, CFYD, CKCF, CKYC, CKYD, YCYD, YCCF, YCCK, YDCF, YDCK, and YDYC) and commercial layer (Hy-Line Brown) were obtained as a counterpart in the study. All the birds are raised in battery cages (76 × 61 × 46 cm3) and then raised until 14 weeks and subsequently moved to layer battery cages (60 × 25 × 45 cm3) and raised until 16 weeks. The body weight and viability were measured biweekly from hatching to week 16. The week of 16, body weight range was about 1,010.24 to 1,411.77 g. The body weight of specific crossbreeds (i.e., CKCF, YCYD, and YDYC) was found to be comparable to that of Hy-Line Brown). The viability hatch to week 14 range was about 55 to 100% and however week 14 to 16 range was 80 to 100%. The crossbred (i.e., CKCF) recorded superior (P<0.05) viability throughout the whole experiment period, even compared with Hy-Line Brown (100% vs 96%). Our results are indicating that crossbreds Korean native chicken including CKCF, and YDYC has the potential to enhance key features of laying hens during the growing phase like optimal body weight and higher viability.

Investigation of Growth and Egg Production Performance of 6 Indigenous Korean Chicken Breeds Enrolled in DAD-IS (DAD-IS에 등재된 한국 토종닭 6품종의 성장 및 산란 능력 조사)

  • Huimang Song;Seungchang Kim;Sang-Rae Cho;Dae-Hyeok Jin
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.241-249
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    • 2023
  • Following the Animal genetic resource for food and agriculture (AnGR) is considered as an independent resource for the possessing country, ensuring the sovereignty of AnGR is important. The present study investigated the growth and egg production performance of six breed enrolled in DAD-IS for the purpose of securing scientific data on AnGR in Korea. A total of 323 chickens (female 181, male 142) were used in this study, with the following six breeds: Korean Leghorn (LEG), Gyeongbuk Araucana (ARA), Korean native chicken (KNC), Korean Ogye (Ogye), Hyunindak (HIL), Heongseongyakdak (HYD). The body weight of male ARA from hatching to 32 weeks of age was the highest among the breeds, and LEG and Ogye were relatively lower (P<0.0001, excluded body weight data of HYD). The body weight of female ARA was the highest and HYD was significantly the lowest among the female chicken breeds (P<0.0001). The laying percentage was the highest in LEG and was the lowest in HYD among the breeds (P<0.0001). The average egg weight from 20 to 40 weeks of age was the highest in ARA, followed by LEG and was the lowest in Ogye (P<0.0001), and the adapted breed including LEG and ARA was higher than indigenous breed (P<0.05). Egg mass production was the highest in Korean Leghorn. Collectively, these results show that ARA has the best growth ability, and LEG has the best egg production performance among the used breeds. This suggests that the adapted breed with high commercial performance is important AnGR in Korea.

Redefinition of the Concept of Fishing Vessel and Legislation Adjustment (낚시어선 개념의 재정립과 법제 정비에 관한 연구)

  • Yeong-Tae Son
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.6
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    • pp.639-652
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    • 2023
  • The fundamental background behind the introduction of the fishing vessel system is to allow petty small fishers to engage in pure fishery business activities with fishing vessels during normal times and engage in fishing vessel business only during specific periods (closed fishing season, etc.) thereby granting a qualification as an auxiliary tool for the economic activities of petty small fishers. In addition, fishing boats are allowed to engage in excursion ship activities using fishing vessels registered under the Fishing Vessels Act, the form of fishing vessels should also have a general and universal structure that is practically easy to engage in fishing activities in the field in accordance with the relevant regulations. However, most fishing vessel proprietors are currently focusing only on increasing income, and rather than building fishing vessels in a reasonable form suitable for the original purpose of general fishing vessels, they prefer an abnormal hull form equivalent to expediency, that is biased hull structure biased toward the fishing vessel business. As a result, it is causing serious problems in safety management as well as conflict [damaging relative equity in government support measures (tax-free oil supply, etc.), and depletion of livelihood-type fish stocks] with fishing vessel forces who consider the fishing vessel business only to be a part of the side job among all fishery business activities. Meanwhile, the most fundamental cause of this problem is that the current Fishing Management and Promotion Act, limits the concept of fishing vessels to fishing vessels registered under the Fishing Vessels Act, and applies survey standards accordingly. Accordingly, in this study, through analysis of the distribution status of fishing vessels, structural characteristics, operation status of fishing vessels, and the government's fishing promotion policies, etc., the relevant laws (regulations) have been reorganized to suit the current reality of the concept of fishing vessels to separate the current fishing vessel from fishing vessels and operate it as a fishing-only vessel.

Analysis of Change in Flora and Vegetation in the Research Sites before and after the Forest Road Construction in Minjujisan in Korea - Focused on the Forest Road at Jeollabuk-do Muju-gun Seolcheon-myeon Micheon-ri Minjujisan Area - (임도 개설 전·후 식물상 및 식생 변화 분석 - 전북 무주군 설천면 미천리 민주지산 임도를 중심으로 -)

  • Hyoun-Sook Kim;Joon-Woo Lee;Sang-Myong Lee
    • Korean Journal of Environment and Ecology
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    • v.37 no.5
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    • pp.367-391
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    • 2023
  • This study was conducted for 10 years from 2012, which is a year before the forest road construction in Minjujisan, to 2022 to analyze annual changes in flora and vegetation before and after the forest road construction and to provide strategies for management. The plant communities in the research sites along the forest road showed the differentiation between slopes with Quercus mongolica community on the northwestern slope and Quercus variabilis and Larix kaempferi communities on the southwestern slope. A total of 212 taxa have increased for number 7 between before and after the construction from a total of 66 taxa (44 families, 59 genera, 51 species, 13 varieties, and 2 forma) in 2012 and 207 taxa (71 families, 153 genera, 176 species, 27 varieties, and 4 forma) in 2015 to 278 taxa (78 families, 172 genera, 242 species, 1 subspecies, 31 varieties, and 4 forma) in 2022. It is noteworthy that the vegetation cover and the introduction of new taxa had been expanded in the sites adjacent to the construction, which is likely caused by the significantly increased amount of light and the introduction of annual herbaceous and naturalized plants after the construction. The results of 10 years of current study reveal that the vegetation cover and the number of new taxa had rapidly increased in earlier years after the construction, slowly decreased later on, and finally formed a stable forest with the increase in the ratio of dominant species. The vegetation cover of the herbaceous layer immediately increased on the slopes along the forest road for a few years after the construction although it had continuously decreased while that of the shrub layer quickly increased. It was shown that on the hillslope the vegetation cover of tall- and low-tree layers increased whereas that of herbaceous and shrub layers rapidly decreased.

Indigenous psychological analysis of delinquency among Korean adolescents: Comparison of adolescents under probation and high school students (한국 일탈 청소년의 토착심리 탐구: 보호관찰 청소년과 일반 청소년의 부모자녀관계에 대한 비교를 중심으로)

  • Young-Shin Park ;Uichol Kim ;Soo Yeon Tak
    • Korean Journal of Culture and Social Issue
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    • v.10 no.1
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    • pp.107-145
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    • 2004
  • This study examines factors that influence delinquency among adolescents under probation using indigenous psychological analysis, focusing specifically on parent-child relationship. A total 268 adolescents under probation and their parents and 251 high school students and their parents participated in the two studies. In the first study, qualitative results indicate that both groups of adolescents were most likely to trust their parents than any other person and reason for their trust is reported to be based on blood relationship. Similarly, majority of parents of both groups reported trusting their children because of the blood relationship. Parents hope that their children will be sincere a person and will be able to maintain harmonious social relationship. Parents of adolescents under probation were more likely to report disobedience as being most problematic, whereas parents of high school students were more likely report providing social and financial support for their children's education as being the most difficult. In the second study, structured questionnaires were administered to the four groups and the following set of results were obtained. First, in terms of family background, the socio-economic status of adolescents under probation was lower, they are less likely to live with both parents or natural parents, and more likely to have ran away from home than high school students. Second, adolescents under probation are less likely to trust their parents and more likely to view their parents as being hostile. Third, parents of adolescents under probation were less likely to trust their children, more likely to view their relationship as being conflictual and hostile, and feel that they had to sacrifice for their children when compared with parents of high school students. Implications of these results for parent-child relationship and delinquency are discussed.

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Investigation of genetic variability in commercial and invaded natural populations of red swamp crayfish(Procambarus clarkii) from South Korea (미국가재(Procambarus clarkii) 수족관 개체군 및 국내 침입 자연개체군의 유전적 변이 연구)

  • Ji Hyoun Kang;Jeong Mi Hwang;Soon-Jik Kwon;Min Jeong Baek;Sun-Jae Park;Changseob Lim;Yeon Jae Bae
    • Korean Journal of Environmental Biology
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    • v.41 no.3
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    • pp.325-334
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    • 2023
  • The invasive red swamp crayfish, Procambarus clarkii, is native to south-central United States and northeastern Mexico. Recently, it has been being spreading in the wild in South Korea. However, its primary sources, introduction routes, establishment, and expansion in South Korea remain unclear. Here, we analyzed genetic diversity and population genetic structures of its domestic natural populations during early invasion, commercial stock from local aquaria (a suspected introduction source), and original United States population using mitochondrial COI gene sequences for 267 individuals and eight microsatellite markers for 158 individuals. Natural and commercial populations of P. clarkii showed reduced genetic diversity (e.g., haplotype diversity and allelic richness). The highest genetic diversity was observed in one original source population based on both genetic markers. Despite a large number of individuals in commercial aquaria, we detected remarkably low genetic diversity and only three haplotypes among 226 individuals, suggesting an inbred population likely originating from a small founder group. Additionally, the low genetic diversity in the natural population indicates a small effective population size during early establishment of P. clarkii in South Korea. Interestingly, genetic differentiation between natural populations and the United States population was lower than that between natural populations and aquarium populations. This suggests that various genetic types from the United States likely have entered different domestic aquariums, leading to distinct natural populations through separate pathways. Results of our study will provide an insight on the level of genetic divergence and population differentiation during the initial stage of invasion of non-indigenous species into new environments.

Psycho-Social Determinants of Subjective Well-being and Physical Health of a Retired Elders in Korea: A Longitudinal Study on the occupational classification (은퇴 노인의 주관안녕과 신체건강에 영향을 미치는 요인들: 은퇴전 직종에 따른 종단 연구)

  • Kun-Seok Park
    • Korean Journal of Culture and Social Issue
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    • v.15 no.2
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    • pp.291-318
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    • 2009
  • The purpose of this study was to find out the impact of psycho-social factors (social relationship and personal) as well as illness history and economic status on physical health and subjective well-being among the retired Koreans elderly. Data were collected from 1,315 elders (mean age = 72.70yrs) residing in Seoul and Chuncheon regional area via interviews(Time 1), and them were re-interviewed two year later(Time 2). Multiple regression analyses indicated that the retired elders' illness history, economic status, marital satisfaction, fulfillment of self-esteem need, drinking behavior, positive affectivity, negative affectivity and physical health to predict their subjective well-being at Time 1(R2=.705). The retired elders' economic status, marital satisfaction, positive affectivity, negative affectivity and physical health to predict their subjective well-being at Time 2(R2=.418). The retired elders' illness history, economic status, expectations for one's offspring, drinking behavior and subjective well-being to predict their physical health at Time 1(R2=.364). And the retired elders' illness history, economic status, marital satisfaction, positive affectivity and negative affectivity to predict their physical health at Time 2(R2=.265). In case of retired elderly, suggested for the psycho-social determenants of subjective well-being and physical health by occupational classification. The implications of this study and the suggestions for furture study were discussed.

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