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Characterization of a new commercial strain 'Guseol' by intra-specific hyphal anastomosis in Pleurotus ostreatus (계통간 교잡에 의한 느타리 품종 '구슬'의 육성 및 그 특성)

  • Yoo, Young-Bok;Kim, Eun-Jung;Kong, Won-Sik;Jang, Kab-Yeul;Shin, Pyung-Gyun
    • Journal of Mushroom
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    • v.10 no.3
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    • pp.109-114
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    • 2012
  • To develop new variety of oyster mushroom, 63 intra-specific hybrids between the strain Suhan and #Nongi201 were developed using hyphal anastomosis technique in 2004. The Po2008-275 hybrid between the dikaryon strain 04-154(Suhan x #Nongi201) and the monokaryon strain derived from ASI2487 were developed using hyphal anastomosis in 2008. The Po2008-275 was shown the best cultural characteristics, selected to be a new variety and named as 'Guseol'. The new commercial strain, 'Guseol' had dark grey pilei and grows well under spring and autumn conditions in Korea. The fruiting bodies of 'Guseol' were of an excellent quality in that not only the stipe was thick and long but also the pileus was small and hard. The optimum temperatures for mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $10{\sim}16^{\circ}C$, respectively. Time period required for the initiation of the first fruiting body was about 3 to 5 days depending on the temperatures. The shape of fruiting body was thin funnel shape. Fruiting body production per box($43{\times}43{\times}12cm$) was about $1545{\pm}400.9g$ which was almost 137% quantity compared to that of parental strain 04-154. Relatively low temperature incubation ($11^{\circ}C$) resulted in the development of better quality of 'Guseol' mushrooms. When two different media including potato dextrose medium and mushroom complete medium were compared, the mycelial growth of this mushroom were much faster in mushroom complete medium. Similar results were observed with other variety '#Chunchu2'. Analysis of the genetic characteristics of the new commercial strain 'Guseol' showed a major DNA profile as that of the parental 04-154 when primer URP 1, primer URP 2 and primer URP 5 were used, but different to '#Chunchu2' that was used as a control. This new variety of the dark grey oyster mushroom had smart and high quality image that corresponds well to "health food". We therefore expect that this new strain will satisfy the consumers demand for variety and excellent mushrooms.

The Analysis of Evergreen Tree Area Using UAV-based Vegetation Index (UAV 기반 식생지수를 활용한 상록수 분포면적 분석)

  • Lee, Geun-Sang
    • Journal of Cadastre & Land InformatiX
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    • v.47 no.1
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    • pp.15-26
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    • 2017
  • The decrease of green space according to the urbanization has caused many environmental problems as the destruction of habitat, air pollution, heat island effect. With interest growing in natural view recently, proper management of evergreen tree which is lived even the winter season has been on the rise importantly. This study analyzed the distribution area of evergreen tree using vegetation index based on unmanned aerial vehicle (UAV). Firstly, RGB and NIR+RG camera were loaded in fixed-wing UAV and image mosaic was achieved using GCPs based on Pix4d SW. And normalized differences vegetation index (NDVI) and soil adjusted vegetation index (SAVI) was calculated by band math function from acquired ortho mosaic image. validation points were applied to evaluate accuracy of the distribution of evergreen tree for each range value and analysis showed that kappa coefficient marked the highest as 0.822 and 0.816 respectively in "NDVI > 0.5" and "SAVI > 0.7". The area of evergreen tree in "NDVI > 0.5" and "SAVI > 0.7" was $11,824m^2$ and $15,648m^2$ respectively, that was ratio of 4.8% and 6.3% compared to total area. It was judged that UAV could supply the latest and high resolution information to vegetation works as urban environment, air pollution, climate change, and heat island effect.

A Descriptive Study on the Tuberculosis Mortality in a Tuberculosis-Centered Hospital (한 결핵전문병원의 입원 결핵환자 사망에 대한 기술통계학적 고찰)

  • Kim, Soo-Young;Byun, Joo-Nam;Choi, Jin-Su
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.5
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    • pp.595-601
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    • 1993
  • Background: Today, tuberculosis continues as an important cause of death in Korea despite the effective treatment and prevention. So we have studied charicteristic distribution of death by pulmonary tuberculosis through epidemiologic survey. Subjects and Method: The mortality data were obtained from 684 pulmonary tuberculosis cases who died in a tuberculosis-centered hospital in Seoul during the period of 5 years from 1986 to 1990. In order to estimate the distribution of death by tuberculosis, t-test and $x^2$-text were performed on the data. Results: 1) 19.9% of patients died among the total 3,441 hospitalized pulmonary tuberculosis cases during 5 years. 2) In distribution of sex and age, male death occupies 81% of total death. Significantly high proportions of younger female death (under 40 years-old) were also observed. 3) In terms of medical security status, medical assistance group occupies 42.3% of medical insurance group while the non-security group also occupies 11.8% of total death. 4) Treatment interruption was observed in 78% of total death. Conclusion: Special attention should be given to the identification, management and follow up of high risk group in nationwide tuberculosis control program.

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Relationships of Fatigue to Job Satisfaction and Turnover Intention among Clinical Nurses at Small & Medium-Sized Hospitals (중소병원 간호사의 피로와 직무만족 및 이직의도와의 관계)

  • Choi, So-Eun;Kim, Sang-Dol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.9
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    • pp.4218-4226
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    • 2013
  • This is a descriptive study to identify relationships of fatigue to job satisfaction and turnover intention among clinical nurses. The subjects were 162 clinical nurses at 2 small and medium-sized hospitals in K and B cities. The data were collected from February 1st throughout 28th, 2013. The collected data were analyzed using the SAS program through $x^2$-test, t-test, ANOVA, Scheff$\acute{e}$ test, and Pearson's correlation coefficients. There was a negative correlation between high fatigue level and job satisfaction and colleagues of its subgroups (r=-0.29, p=.0214; r=-0.29, p=.0239), positive correlation it and turnover intention(r=0.33, p=.0089). Also there was a negative correlation between promotion and overall job satisfaction subgroup of job satisfaction and turnover intention(r=-0.17, p=.0268; r=-0.19, p=.0144). Therefore nurse managers are in need of applying the organizational management system which is considered the relationship with my colleagues at work, promotion and overall job satisfaction etc. to prevent and decrease fatigue induced factors and turnover intentions.

A Analysis of Effectiveness of Aluminium Filter in the added Compound Filtration by Detective Quantum Efficiency and Image Quality Evaluation (복합부가여과에서 알루미늄 여과판 사용 시 양자검출효율과 화질평가를 통한 효율성 분석)

  • Kim, Sang-Hyeon;Kim, Yun-Min;Kwon, Kyoung-Tae;Ma, Sang-Chull;Han, Dong-Gyoon
    • The Journal of the Korea Contents Association
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    • v.15 no.10
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    • pp.362-373
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    • 2015
  • This study analysed the effectiveness of aluminium(Al) filter in the added compound filtration for the removal of characteristic radiation from high atomic number material by DQE and image evaluation. 1mm Al was applied to each 0.1, 0.2, 0.3 mm copper and befere and after use were evaluated. Beam quality and DQE were tested by IEC regulations and image quality was evaluated by PSNR, MAE, MSE, CNR, SNR and qualitative analysis was performed by 7 items for resolution and contrast from chest x-ray criteria of national cancer checkup. MTF 10 and 50% were the same by 4.6, 2.54 cycle/mm and NPS, DQE, PSNR MAE, MSE, CNR, SNR and qualitative analysis were all the same or slightly better when Al was not used. PSNR is over 30dB and all significant and at the qualitative analysis, the p-value of t-test was over 0.05. The DQE and image quality evaluation have little difference between before and after use of Al filter and it is effective to use the Al filter for the reduction of skin dose by removal of characteristic radiation.

Influences of Silicate Fertilizer Application on Soil Properties and Red Pepper Productivity in Plastic Film House (규산질비료가 시설재배지 토양특성과 고추수량에 미치는 영향)

  • Ahn, Byung-Koo;Han, Soo-Gon;Kim, Jong-Yeob;Kim, Kab-Cheol;Ko, Do-Young;Jeong, Seong-Soo;Lee, Jin-Ho
    • Korean Journal of Environmental Agriculture
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    • v.33 no.4
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    • pp.254-261
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    • 2014
  • BACKGROUND: This study was conducted to investigate effects of silicate fertilizer application on red pepper (Capsicum annuum L.) productivity with improving soil chemistry under plastic film house in paddy field. METHODS AND RESULTS: The silicate fertilizer was applied as 0, 100, 200, and 300 kg/10a as basal dressing before transplanting pepper plant seedlings. Cultivar of the pepper plant was Cheon-Ha-Dae-Se. Amounts of inorganic fertilizer applied as $N-P_2O_5-K_2O$=19.0-6.4-10.1kg/10a was estimated depending on soil test values. After applying 50% of nitrogen, 100% of phosphorus, and 60% of potassium fertilizers as basal dressing, the seedlings of pepper plant were transplanted. The rests of nitrogen and potassium fertilizers were applied as side-dressing after the first, second, and fourth harvests of red pepper. When comparing selected chemical properties of soils between before transplanting and after final(the fifth) harvest, soil pH, available $P_2O_5$, and exchangeable $Ca^{2+}$ increased with increasing the applications of silicate fertilizer, whereas electrical conductivity(EC) decreased. However, exchangeable $K^+$ was higher with the treatments of 100 and 200 kg/10a, and exchangeable $Mg^{2+}$ was higher with 300 kg/10a application. In addition, nitrogen and phosphorus concentrations of red pepper collected from the first harvesting stage decreased with increasing the applications of silicate fertilizer, but potassium, calcium, and magnesium concentrations in red pepper were highest with 300 kg/10a application. Yield of red pepper increased between 9.0 and 11.8% with the applications of silicate fertilizer. Marketable fruit rate of res pepper was highest(97.3%) with 200 kg/10a application. CONCLUSION: The application of silicate fertilizer as basal dressing in paddy-converted fields improved soil chemistry and increased red pepper productivity.

MICROLEAKAGE OF THE CLASS V CAVITY ACCORDING TO RESTORATION SITE AND CAVITY SIZE USING SEM AND THREE-DIMENSIONAL RECONSTRUCTION TECHNIQUES (SEM과 3차원 재구성법을 이용한 수복면의 위치와 와동 크기에 따른 미세누출도 분석)

  • Yang, In-Seo;Shin, Dong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.30 no.2
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    • pp.112-120
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    • 2005
  • This study was done to evaluate whether there were any differences in microleakage of class V composite restorations according to restoration site and cavity size. Total sixty-four restorations were made in molar teeth using Esthet-X. Small ($2\;{\times}\;2\;{\times}\;1.5\;mm$) and large ($4{\times}2{\times}1.5\;mm$) restorations were made at the buccal/lingual surface and the proximal surface each. After 1,000 times of thermocycling ($5^{\circ}\;-\;55^{\circ}C$), resin replica was made and the percentage of marginal gap to the whole periphery of the restoration was estimated from SEM evaluation. Thermocycled tooth was dye penetrated with $50\%$ silver nitrate solution. After imbedding in an auto-curing resin, it was serially ground with a thickness of 0.25 mm. Volumetric microleakage was estimated after reconstructing three dimensionally. Two-way ANOVA and independent T-test for dye volume, Mann-Whitney U test for the percentage of marginal gap, Spearman's rho test for the relationship between two techniques were used, The results were as follows : 1. The site and size of the restoration affected on the microleakage of restoration. Namely, much more leakage was seen in the proximal and the large restorations rather than the buccal/lingual and the small restorations. 2. Close relationship was found between two techniques (Correlation coefficient = 0.614/ P = 0.000). Within the limits of this study, it was noted that proximal and the large restorations leaked more than buccal/lingual and the small restorations. Therefore, it should be strictly recommended large exposure of margins should be avoided by reducing unnecessary tooth reduction.

Comparison of shaping ability between single length technique and crown-down technique using Mtwo rotary file (Mtwo 전동 파일을 사용한 single length technique과 crown-down technique의 근관성형 효율 비교)

  • Lim, Yoo-Kyoung;Park, Jeong-Kil;Hur, Bock;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.32 no.4
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    • pp.385-396
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    • 2007
  • The aims of this study were to compare the shaping effect and safety between single length technique recommended by manufacturer and crown-down technique using Mtwo rotary file and to present a modified method in use of Mtwo file. Sixty simulated root canal resin blocks were used. The canals were divided into three groups according to instrument and the manner of using methods. Each group had 20 specimens. Group MT was instrumented with single length technique of Mtwo, group MC was instrumented with crown-down technique of Mtwo and group PT was instrumented with crown-down technique of ProTaper. All of the rotary files used in this study were operated by an electric motor. The scanned canal images of before and after preparation were superimposed. These superimposed images were evaluated at apical 1 to 8 mm levels Angle changes were calculated. The preparation time, weight loss, instrument failure and binding, canal aberrations, and centering ratio were measured. Statistical analysis of the three experimental groups was performed with ANOVA and Duncan's multiple range tests for post-hoc comparison and Fisher's exact test was done for the frequency comparison. In total preparation time, group MT and group MC were less than group PT. In the aberrations, group MT had more elbows than those of group MC and group PT. The binding of group MC was least and group MT was less than group PT (P < 0.05). Under the condition of this study, crown-down technique using Mtwo rotary file is better and safer method than single length technique recommended by the manufacturer.

Characteristics of New Ever-bearing Strawberry 'Bokha' Bred for Tropical and Subtropical zone Cultivation (열대 및 아열대 지역 재배용 사계성 딸기 '복하' 육성)

  • Lee, Jong Nam;Kim, Hye Jin;Choi, Mi Ja;Suh, Jong Taek;Nam, Jung Hwan;Hong, Su Young;Kim, Su Jeong;Shon, Hwang Bae;Kim, Ki Deog;Kim, Yul Ho
    • Journal of the Korean Society of International Agriculture
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    • v.30 no.4
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    • pp.300-304
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    • 2018
  • 'Bokha' is a new strawberry (Fragaria x ananassa Duch.) cultivar, which was released by the Highland Agriculture Research Institute in 2016. The 'Bokha' cultivar originated from a cross between 'Goha' and 'Saebong No. 3' that showed excellent ever-bearing characteristics, including continuous flowering habit and high soluble-solids contents under long-day and high temperature conditions in 2011. This cultivar was initially named 'Saebong No. 8' after examining its characteristics and productivity in summer culture from 2013 to 2015. After regional adaptability tests in 2016, 'Bokha' was selected from Saebong No. 8 as an elite cultivar. The general characteristics of 'Bokha' include semispreading type, elliptical leaves, and moderately vigorous growth. The fruits are conical in shape, and red in color. 'Bokha' plants have 32.7 leaves, 9.9 more than 'Goha' plants. The soluble-solids content of 'Bokha' was 9.2%, which was 0.2% higher than that of 'Goha'. The average fruit weight of 'Bokha' was about 9.5g and the marketable yield was $27,701kg{\cdot}ha^{-1}$, 72% higher than 'Goha'. 'Bokha' is suitable for tropical and subtropical zone cultivation as a high soluble solids contents cultivar, because it shows continuous flowering habit under long-day and high temperature conditions.

A Study on Dental Caries Activity Assessment from Saliva of Students of Dentistry College (I) (치과대학 대학생 타액의 치아 우식활성도 평가(I))

  • Son, Seung Hwa;Kim, Dong Ae;Park, Young Min
    • Journal of dental hygiene science
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    • v.13 no.2
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    • pp.182-190
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    • 2013
  • Composition of oral microbial flora is suggested to play a role in the maintenance of oral health. Among them, Streptococcus mutans plays an essential role in the formation of dental plaque and it is being noticed as incipient infective bacteria of dental caries. The purpose of this study was to prepare the basic data about distribution of Streptococcus mutans detected from saliva by measuring colony density. It is well known that smoking as well as drinking is a factor of dental caries, however there are few investigations about distribution of Streptococcus mutans either smoking or drinking. The materials of this study were collected from students of dentistry college with general characteristics obtained by self-administered questionnaires. Students were asked to gently chew the paraffin to get saliva for 1 minute. Bacitracin disc in the culture medium was activated for 15 minutes, and then sample was incubated in the activated medium at $37^{\circ}C$ incubator for 48 hours. Streptococcus mutans colonization per 1 ml saliva was measured by the number of criteria. The severe levels of dental caries activity were significantly increased in the case of smoking (from 5.6% to 10%) or drinking (form 0% to 7%), respectively. Also, dental caries activity increased according to smoking and drinking periods longer. However, using the SPSS analytical process (Mann-Whitney's U), no significant differences were observed between the smoking and the non-smoking or the drinking and non-drinking, respectively. These results obtained from dental caries activity of saliva suggest that distribution of Streptococcus mutans following smoking or drinking can be used as a significant information for oral health.