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Studies on the fiber contents and effective selection method by the early testing in thirteen different hemp varieties (조기검정법에 의한 대마의 섬유함량 및 선발에 관한 연구)

  • Johng-Moon Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.3
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    • pp.99-107
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    • 1965
  • Thirteen varieties of hemp, Cannabinus sativa L., consisting of four Italian, one Japanese and eight local varieties were used in the study on the fiber content and a satisfactory selection for the higher-fiber-yielding plants. In addition, the relationship among fiber contents at the ball setting stage, and at the maturity stage, primary fiber content, and total fiber content were determined. The results of the study are summarized as follow: 1. Fiber weight of the matured male plant expressed as percentage of the fiber weight to dried stems varied from 12.09 to 26.71 percent for the Italian varieties, 14.25% for the Japanese variety and ranged from 6.59 to 16.79 for the local varieties. It was shown that on the average, Italian varieties contained more fiber compared with the other varieties. 2. On the same basis, the average percent age of fiber for Italian male plants was 20.77 while it was 20.96 for female plants. For the Japanese variety, similar figures were 14.24 for male plants and 15.43 for female plants. By comparison, the average percent fiber for the male plants of Korean varieties was only 10.34 but averaged 16.79 for female plants. In this experiment, female plants contained a greater percentage of fiber than male plants. 3. Statistically significant correlations were obtained between fiber content at the ball setting stage and fiber content of the mature plant. The correlation coefficient was r=0.942**, while the regression equation was Y=0.28+1.03X. These data indicate that plant. selection by early testing at the ball setting stage is an effective method for the improvement of fiber content. 4. The correlation between primary fiber content and total fiber content was r=0.913**, with a regression equation of Y=12.01+1.20X. It should thus be possible to obtain plants of higher total fiber content at maturity by the selection of plants which have a higher content of primary fiber at the ball setting stage. 5. From a study of the transverse sections from the hemp plant it was shown that Italian varieties have wide bands of primary and secondary fibers with narrow areas of woody material, while both Korean local varieties and Japanese varieties have narrow bands of primary and secondary fiber and wide areas of woody material. For hemp improvement, it is certain that a variety which has a narrow area of woody substances and wide area of fiber in its stem should be selected.

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Investigation of Tube Voltage Range using Dose Comparison based on Effective Detector Exposure Index in Chest Radiography (흉부 X-ray 검사 시 선량 비교를 활용한 유효 Detector Exposure Index 기반의 적절한 관전압 범위 제안)

  • Shim, Jina;Lee, Youngjin
    • Journal of the Korean Society of Radiology
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    • v.15 no.2
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    • pp.139-145
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    • 2021
  • This study is to confirm the range of tube voltage for Chest X-ray in DR system by comparing with dose area product (DAP) and effective dose in efficient detector exposure index (DEI) range. GE definium 8000 was used to for the phantom study. The range of tube voltage is 60~130 kVp and of mAs is 2.5~40 mAs. The acquired images were classified into efficient DEI groups, then calculated effective dose with DAP by using a PC-Based Monte Carlo Program 2.0. The signal to noise ratio (SNR) was measured at 4 regions, including the thoracic spine, the lung area with the ribs, the lung area without the ribs, and the liver by using Picture Archiving and Communication System. The significance of the group for each tube voltage was verified by performing the kruskal-wallis test and the mann-whitney test as a post-test. When set to 4 groups dependned on the tube voltage, DAP showed significant differences; 60 kVp and 80 kVp, and 60 kVp and 90 kVp (p= 0.034, 0.021). Effective dose exhibited no statistically significant differences from the all of the group (p>0.05). SNR exhibited statistically significant differences from the all of the group in the liver except compared to 80 kVp and 90 kVp (p<0.05). Therefore, high tube voltages of 100 kVp or more need to be reconsidered in terms of patient dose and imaging in order to represent an appropriate chest X-ray image in a digital system.

The Effect of Addition of Egg Yolk and Sucrose on the pH and Specific Gravity for Heated Egg Albumen (난백의 가열처리에 있어서 난황과 자당 첨가가 pH 및 비중의 변화에 미치는 영향)

  • 황경규;양기원;하정기
    • Korean Journal of Poultry Science
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    • v.19 no.1
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    • pp.1-12
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    • 1992
  • This study was divided into experiment I(129g egg-albumen plus 150g sucrose) and experiment II(161g egg-albumen plus 150g sucrose) which were subdivided into groups treated with 0, 8.71, 17.43 and 26.149 egg yolk. These experiments were incubated in a shaking water bath(5$0^{\circ}C$) with a speed of 92(turnaround) per minute for a period of times. The pH and specific gravity were measured after 2, 4, 6, 8, 10 and 12 hour incubations. The results obtained were as follows: 1. The pH and specific gravity were steadily increased by shaking time, but variably was influenced by the amount of egg yolk and egg-albumen. 2. In the groups treated with egg-albumen, egg yolk, and sucrose of experiments I and II, pH was increased by the amount of egg-albumen in conjunction with shaking time, but specific gravity was decreased. 3. Ten hours after shaking maximum pH 8.60 was shown in the groups treated with 161g egg-albumen : however, in :he groups treated with 129g egg-albumen the maximum pH 8.39 was shown 12 hours after shaking. The different time exhibited maximum pH resulted from the amount of egg-albumen used. 4. The pH specific gravity were higher in the ,groups treated without egg yolk than in the groups with egg yolk. 5. In the experiment I which was added four levels of egg yolk to 1509 of sucrose and 129g of egg albumen, specific gravity(Table 2) had a high (r=0.9692$^{* *}$) correlation with pH (Table 1) and the regression equation between specific gravity and pH (X) was Y=0.050+0.145x. 6. In the experiment II which was added four levels of egg yolk to 150g of sucrose and 161g of egg albumen, specific gravity(Table 4) had a high (r=0.8963$^{* *}$) correlation with pH (Table 3) and the regression equation between specific gravity and pH (X) was Y=0.294+0.110X.10X.

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Developmental Rate Equations for Predicting Blooming Date of 'Yumyeong' (Prunus persica) Peach Trees (발육 속도 모델을 이용한 복숭아 '유명'의 개화기 예측)

  • Yun, Seok Kyu;Chung, Kyeong Ho;Yoon, Ik Koo;Nam, Eun Young;Han, Jeom Hwa;Yu, Duk Jun;Lee, Hee Jae
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.4
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    • pp.189-195
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    • 2012
  • To predict the blooming date of 'Yumyeong' peach trees, the models for flower bud developmental rate (DVR) were constructed. The DVRs were calculated from the demanded times at controlled air temperatures. The branches of 'Yumyeong' peach trees were incubated at three different temperatures of 9.7, 15.2, and $18.9^{\circ}C$. The DVRs were also constructed with blooming dates and air temperatures in the field, collected from 1979 to 2008 at the experimental orchard of National Institute of Horticultural and Herbal Science, Suwon, Korea. All the DVRs increased linearly or exponentially with air temperature. The DVR equations evaluated under controlled air temperatures were y=0.0018x+0.0051 and y=$0.0125e^{0.0603x}$. The DVR equations under field conditions were calculated as y=0.0039x-0.0112 and y=$0.0062e^{0.1512x}$. These DVR equations offer developmental indices and predict the date for blooming with air temperature data. These DVR equations were validated against the blooming data observed in the field. When the blooming dates were calculated with exponential DVR equations and daily air temperature data, the root mean squared errors between the observed and predicted dates were around 2 days. These results suggest that the DVR models are useful to predict the blooming date of 'Yumyeong' peach trees.

Effect of curing modes on micro-hardness of dual-cure resin cements (중합방법이 이중중합 레진시멘트의 미세경도에 미치는 영향)

  • Lee, Ki-Deok;Park, Se-Hee;Kim, Jin-Woo;Cho, Kyung-Mo
    • Restorative Dentistry and Endodontics
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    • v.36 no.2
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    • pp.132-138
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    • 2011
  • Objectives: The purpose of this study was to evaluate curing degree of three dual-cure resin cements with the elapsed time in self-cure and dual-cure mode by means of the repeated measure of micro-hardness. Materials and Methods: Two dual-cure self-adhesive resin cements studied were Maxcem Elite (Kerr), Rely-X Unicem (3M ESPE) and one conventional dual-cure resin cement was Rely-X ARC resin cement (3M ESPE). Twenty specimens for each cements were made in Teflon mould and divided equally by self-cure and dual-cure mode and left in dark, $36^{\circ}C$, 100% relative humidity conditional-micro-hardness was measured at 10 min, 30 min, 1 hr, 3 hr, 6 hr, 12 hr and 24 hr after baseline. The results of micro-hardness value were statistically analyzed using independent samples t-test and one-way ANOVA with multiple comparisons using Scheffe's test. Results: The micro-hardness values were increased with time in every test groups. Dual-cure mode obtained higher micro-hardness value than self-cure mode except after one hour of Maxcem. Self-cured Rely-X Unicem showed lowest value and dual-cured Rely-X Unicem showed highest value in every measuring time. Conclusions: Sufficient light curing to dual-cure resin cements should provided for achieve maximum curing.

Characterization of Oszinc626, knock-out in zinc finger RING-H2 protein gene, in Ac/Ds mutant lines of rice(Oryza sativar L.) (Zinc finger RING-H2 protein관련 Ac/Ds전이인자 삽입 변이체 Oszinc626 유전자의 특성 분석)

  • Park, Seul-Ah;Jung, Yu-Jin;Ahn, Byung-Ohg;Yun, Doh-Won;Ji, Hyeon-So;Park, Yong-Hwan;Eun, Moo-Young;Suh, Seok-Cheol;Lee, Soon-Youl;Lee, Myung-Chul
    • Journal of Plant Biotechnology
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    • v.35 no.3
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    • pp.177-183
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    • 2008
  • Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.

Chitinase을 생산하는 곤충병원미생물 Metarhizium anisopliae HY-2(KCTC 0156BP)의 토양해충 생물검정

  • Seo, Eun-Yeong;Son, Gwang-Hui;Sin, Dong-Ha;Kim, Gi-Deok;Park, Du-Sang;Park, Ho-Yong
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.469-472
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    • 2002
  • Solid state fermentation was performed for the production of entomopathogenic fungus Metarhizium anisopliae HY-2 using wheat bran media containing rice bran. Fungal growth in a solid state fermentation system was estimated by viable cell count, spore count, and mycelial biomass. It was used chemical method measuring N-acetyl-glucosamine (chitin) content for estimating of mycelial biomass. In static flask culture, viable cell reached 2.40 ${\times}$ $10^8$ cfu/g at 23 days of culture at $27^{\circ}C$ and then mycelial biomass was 41.59 mg/g. Specific growth rate(${\mu}$ max) was 0.0418 $h^{-1}$ between 3 and 9 days when estimated by viable cell count and was 0.00976 $h^{-1}$ between 9 and 17 days when N-acetylglucosamine content was measured. Viable cells reached 1.12 ${\times}$ $10^8$ cfu/g in polypropylene-bag at 28 days of culture at $27^{\circ}C$. Formulated microbial pesticide containing M. anisopliae HY-2 were tested their bio-activity against Chestnut Brown Chafer (Adoretus tenuimaculatus). The protection rate of the liquid culture showed 13 ${\sim}$ 26 % with 1st to 3rd instar, and spore suspension of M. anisopliae HY-2 showed 56 ${\sim}$ 64%. Conidia produced by large scale solid-state fermentation showed 20 ${\sim}$ 27 % activity 60 ${\sim}$ 64 % with M. anisopliae HY-2.

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Studies on Garlic Mosaic Virus -lts isolation, symptom expression in test plants, physical properties, purification, serology and electron microscopy- (마늘 모자이크 바이러스에 관한 연구 -마늘 모자이크 바이러스의 분리, 검정식물상의 반응, 물리적성질, 순화, 혈청반응 및 전자현미경적관찰-)

  • La Yong-Joon
    • Korean journal of applied entomology
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    • v.12 no.3
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    • pp.93-107
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    • 1973
  • Garlic (Allium sativum L.) is an important vegetable crop for the Korean people and has long been cultivated extensively in Korea. More recently it has gained importance as a source of certain pharmaceuticals. This additional use has also contributed to the increasing demand for Korean garlic. Garlic has been propagated vegetatively for a long time without control measures against virus diseases. As a result it is presumed that most of the garlic varieties in Korea may have degenerated. The production of virus-free plants offers the most feasible way to control the virus diseases of garlic. However, little is known about garlic viruses both domestically and in foreign countries. More basic information regarding garlic viruses is needed before a sound approach to the control of these diseases can be developed. Currently garlic mosaic disease is most prevalent in plantings throughout Korea and is considered to be the most important disease of garlic in Korea. Because of this importance, studies were initiated to isolate and characterize the garlic mosaic virus. Symptom expression in test plants, physical properties, purification, serological reaction and morphological characteristics of the garlic mosaic virus were determined. Results of these studies are summarized as follows. 1. Surveys made throughout the important garlic growing areas in Korea during 1970-1972 revealed that most of the garlic plants were heavily infected with mosaic disease. 2. A strain of garlic mosaic virus was obtained from infected garlic leaves and transmitted mechanically to Chenopodium amaranticolor by single lesion isolation technique. 3. The symptom expression of this garlic mosaic virus isolate was examined on 26 species of test plants. Among these, Chenopodium amaranticolor, C. quince, C. album and C. koreanse expressed chlorotic local lesions on inoculated leaves 11-12 days after mechanical inoculation with infective sap. The remaining 22 species showed no symptoms and no virus was recovered from them whet back-inoculated to C. amaranticolor. 4. Among the four species of Chtnopodium mentioned above, C. amaranticolor and C. quinoa appear to be the most suitable local lesion test plants for garlic mosaic virus. 5. Cloves and top·sets originating from mosaic infected garlic plants were $100\%$ infected with the same virus. Consequently the garlic mosaic virus is successively transmitted through infected cloves and top-sets. 6. Garlic mosaic virus was mechanically transmitted to C, amaranticolor when inoculations were made with infective sap of cloves and top-sets. 7. Physical properties of the garlic mosaic virus as determined by inoculation onto C. amaranticolor were as follows. Thermal inactivation point: $65-70^{\circ}C$, Dilution end poiut: $10^-2-10^-3$, Aging in vitro: 2 days. 8. Electron microscopic examination of the garlic mosaic virus revealed long rod shaped particles measuring 1200-1250mu. 9. Garlic mosaic virus was purified from leaf materials of C. amaranticolor by using two cycles of differential centrifugation followed by Sephadex gel filtration. 10. Garlic mosaic virus was successfully detected from infected garlic cloves and top-sets by a serological microprecipitin test. 11 Serological tests of 150 garlic cloves and 30 top-sets collected randomly from seperated plants throughout five different garlic growing regions in Korea revealed $100\%$ infection with garlic mosaic virus. Accordingly it is concluded that most of the garlic cloves and top-sets now being used for propagation in Korea are carriers of the garlic mosaic virus. 12. Serological studies revealed that the garlic mosaic virus is not related with potato viruses X, Y, S and M. 13. Because of the difficulty in securing mosaic virus-free garlic plants, direct inoculation with isolated virus to the garlic plants was not accomplished. Results of the present study, however, indicate that the virus isolate used here is the causal virus of the garlic mosaic disease in Korea.

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Changes of Isoflavone Contents During Maturation under Different Planting Dates in Black Soybean (파종기 차이에 따른 등숙기간 중 검정콩의 아이소플라본 함량 변화)

  • Yi, Eun-Seob;Yoon, Seong-Tak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.4
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    • pp.424-429
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    • 2012
  • This study was carried out to investigate the influence of different planting time on the synthesis of isoflavone in black soybean, Three varieties used in this experiment were lpumgeomjeongkong, Cheongjakong and Heugcheongkong which had different ecotypes, repectively. Seeds were sown at different time, May 15th, May 30th and June 15th with planting density of $60{\times}15cm$. In order to analyze the content of isoflavone, we collected sample every 5 days from 30 days after flowering to harvest and analyzed them with UPLC. As sowing was delayed, the content of isoflavone increased in all of three varieties. The content of genistein was greater than daidzein and glycitein. Increase of Glycitein was not distinct from 55 days after flowering(DAF) and it was stable against temperature change during the seed developing period. Although the content of genistein in Ilpumgeomjeongkong from 50 to 55 DAF, in Cheongjakong from 40 to 55 DAF and in Heugcheongkong from 60 to 65 DAF was lower than the content of daidzein, it was higher than that of daidzein afterward. In the statistical analysis on the relationship between average temperature and the content of aglycone isoflavone at 5-day intervals from 30 DAF during the grain filling period, genistein in Ilpumgeomjeongkong showed meaningful correlation as y=-15.28x+407.9 ($R^2=0.505^*$), diadzein in Cheongjakong showed meaningful correlation as y=-6.188x-164.5($R^2=0.454^*$), and genistein showed significantly high correlation as y=-11.59x+297.6 ($R^2=0.545^{**}$). Taking all the above results into consideration, it was suggested that the regions suitable for high content of isoflavone in black soybean be the northern area of Gyeonggi-do and Gangwon-do, Chungcheongbuk-do and inland area of Gyeongsangbuk-do, where are relatively low average temperature from flowering stage($R_2$) during the grain filling period.

Variation in Fruit Characteristics of 3x Progenies Obtained from a Cross between 4x and 2x Grape Cultivars (4배체와 2배체 포도 품종간 교배를 통해서 생성된 3배체 후대개체에서 과실특성의 변이)

  • Heo, Jae-Yun;Park, Sung-Min
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.761-770
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    • 2016
  • This study investigated the variation in fruit characteristics between different triploid progenies derived from a cross between tetraploid and diploid grape cultivars, with the aim of developing high quality, labor-saving triploid grapes. No differences were found in fruit quality traits such as soluble solids content and titratable acidity, whereas significant variation was noted in cluster weight induced by a one-time application of GA3 (100 ppm) on the triploid progeny of different cross combinations. The cluster weight was higher in progeny obtained from 'Kyoho' ${\times}$ 'Sekirei' than for offspring derived from other cross combinations; deviation in the mean cluster weight was also small for 'Kyoho' ${\times}$ 'Sekirei' progeny. These results suggest that specific cross combinations, such as 'Kyoho' ${\times}$ 'Sekirei', can be used to efficiently breed new, labor-saving seedless grape cultivars.