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Effect of freezing on electrical properties and quality of thawed chicken breast meat

  • Wei, Ran;Wang, Peng;Han, Minyi;Chen, Tianhao;Xu, Xinglian;Zhou, Guanghong
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.4
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    • pp.569-575
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    • 2017
  • Objective: The objective of this research was to study the electrical properties and quality of frozen-thawed chicken breast meat and to investigate the relationship between these parameters at different times of frozen storage. Methods: Thawed samples of chicken breast muscles were evaluated after being kept in frozen storage at $-18^{\circ}C$ for different periods of time (1, 2, 3, 4, 5, 6, 7, and 8 months). Results: The results showed that water-holding capacity (WHC) and protein solubility decreased while thiobarbituric acid-reactive substances content increased with increasing storage time. The impedance module of samples decreased during 8-month frozen storage. Pearson correlation coefficients showed that the impedance change ratio (Q value) was significantly (p<0.05) related to pH, color, WHC, lipid oxidation and protein solubility, indicating a good relationship between the electrical properties and qualities of frozen-thawed chicken breast meat. Conclusion: Impedance measurement has a potential to assess the quality of frozen chicken meat combining with quality indices.

The Site-Directed A184S Mutation in the HTH Domain of the Global Regulator IrrE Enhances Deinococcus radiodurans R1 Tolerance to UV Radiation and MMC Shock

  • Zhang, Chen;Zhou, Zhengfu;Zhang, Wei;Chen, Zhen;Song, Yuan;Lu, Wei;Lin, Min;Chen, Ming
    • Journal of Microbiology and Biotechnology
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    • v.25 no.12
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    • pp.2125-2134
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    • 2015
  • IrrE is a highly conserved global regulator in the Deinococcus genus and contributes to survival from high doses of UV radiation, ionizing radiation, and desiccation. Drad-IrrE and Dgob-IrrE from Deinococcus radiodurans and Deinococcus gobiensis I-0 each share 66% sequence identity. However, Dgob-IrrE showed a stronger protection phenotype against UV radiation than Drad-IrrE in the D. radiodurans irrE-deletion mutant (ΔirrE), which may be due to amino acid residues differences around the DNA-binding HTH domain. Site-directed mutagenesis was used to generate a Drad-IrrE A184S single mutant, which has been characterized and compared with the ΔirrE mutant complemented strain with Drad-irrE, designated ΔirrE-E. The effects of the A184S mutation following UV radiation and mitomycin C (MMC) shock were determined. The A184S mutant displayed significantly increased resistance to UV radiation and MMC shock. The corresponding A184 site in Dgob-IrrE was inversely mutated, generating the S131A mutant, which exhibited a loss of resistance against UV radiation, MMC shock, and desiccation. qPCR analysis revealed that critical genes in the DNA repair system, such as recA, pprA, uvrA, and ddrB, were remarkably induced after UV radiation and MMC shock in the ΔirrE-IE and A184S mutants. These data suggested that A184S improves the ability against UV radiation and MMC shock, providing new insights into the modification of IrrE. We speculated that the serine residue may determine the efficiency of DNA binding, leading to the increased expression of IrrE-dependent genes important for protection against DNA damage.

Effects of Energy Input and Air Flow Rate on Oxygen Transfer Rate at Different MLVSS in a Jet Loop Reactor (JLR) (MLVSS에 따른 Jet Loop Reactor (JLR)에서 동력량과 공기량이 산소전달률에 미치는 영향)

  • Yoon, Ae-Hwa;Bae, Jong-Hun;Lim, Hyun-Woo;Jun, Hang-Bae;Huh, Tae-Young
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.12
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    • pp.868-873
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    • 2011
  • Oxygen transfer rate generally determines the performance of an aerobic wastewater treatment process that treats high strength wastewater such as food wastewater, animal wastewater and landfill leachate. In this paper, OUR and $K_L{\cdot}a$ were evaluated by using Jet Loop Reactor (JLR) according to the concentration of a mixed liquor volatile suspended solid (MLVSS), oxygen (air) flow rate and energy input as the variable of the operating conditions. Also, a nonlinear regression model was proposed by the statistical methods with the calculated $K_L{\cdot}a$. As a results, in case of applying the high strength wastewater which has to maintain high MLVSS, the energy input and the air flow rate are major parameters oxygen transfer rate in JLR. Finally, the final nonlinear regression model had been developed as a function of E/V, $Q_g$, and ${\mu}_c$.

A Key Pre-distribution Scheme Using Double Hash Chain for Strong Security Strength of Wireless Sensor Node (무선 센서 노드의 강한 보안 강도를 위해 이중 해쉬 체인을 적용한 키 사전 분배 기법)

  • Jeong, Yoon-Su;Kim, Yong-Tae;Park, Gil-Cheol;Lee, Sang-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.8C
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    • pp.633-641
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    • 2008
  • Since WSNs encounter attacks, such as jamming or eavesdropping without physical access occurs, security is one of the important requirements for WSNs. The key pre-distribution scheme that was recently researched for advance of security in WSNs distributes the keys and probability with the use of q-composite random key pre-distribution method, but there is a high probability that no key shared between sensor nodes, and it takes a lot of time and energy to find out the shared key. Therefore, it is not suitable for WSNs. In order to enhance stability of a node that plays a role of gateway without depending on probabilistic key, this paper proposesa key pre-distribution scheme combined with random key pre-distribution scheme and double hash chain. Since the proposed scheme can maintain a small storage place and strong security strengths, it is more efficient than the existing schemes with the same security strengths. In addition, since it uses a small size of key generation key set, it can reduce a great deal of storage overhead.

Effect of antibacterial effects of myrrh, rhatany, chamomomilla against to oral microorganisms (몰약, 라타니아, 카모밀레 등의 구강 내 병원균에 대한 항균작용)

  • Baek, Han-Seung;Kang, Soo-Kyung;Auh, Q-Schick;Chun, Yang-Hyun;Hong, Jung-Pyo
    • Journal of Oral Medicine and Pain
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    • v.38 no.4
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    • pp.299-312
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    • 2013
  • Even though there exist a lot of study about antibacterial effects and reactions of extracted materials from plant, few study exist about oral pathogenic bacteria. Therefore we tried to recognize about the suppression effect to the periodontal pathogenic bacteria and halitosis, when add some kinds of plant extracted materials, myrrh, rhatany, chamomolilla to saliva. We used Crude drug : Myrrh tincture (100mg/ml), Ratanhia tincture (100mg/ml), Chamomile tincture(100mg/ml). We inspected about the cariogenic bateriae, S. mutans GS5 and S. sobrinus 6715, periodontal pathogenic bacteria, P. gingivalis 2561, P. intermedia ATCC 25611, Candida albicans ATCC 18804, and E. feacalis ATCC 4083, then the result follow. The plant extracted material, myrrh, rhatany, chamomomilla, which have convergence effect, bacteriocidal effect and anti-inflammation effect, show an antibacterial effect and reaction to the oral pathogenic bacteria. And with treating rhatany that have the most strong antibacterial effect, through transmission electron microscopy we could see a severe morphologic change of bacteria. This means with the plant extracted material, we can suppress the oral harmful bacteria and prevent periodontal diseases, caries, halitosis and oral inflammations. And within the future studies for the improvement of oral hygiene, our result might be a clinical evidence.

Flora and Vegetation of Mt. Gwaebyung and Galmi-bong, Gangwon Province, Korea (강원도 괘병산과 갈미봉 일대의 식물상과 식생)

  • Kim, Yoo-Shin;Kim, Nam-Young;Kim, Young-Seol;Lee, Hak-Bong;Kim, Se-Chang;Kim, Ji-Hong;Park, Wan-Geun
    • Journal of Korean Society of Forest Science
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    • v.101 no.2
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    • pp.226-235
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    • 2012
  • Floristic composition and phytosociological studies in Mt. Gwaebyung and Galmi-Bong were investigated to classify forest vegetation and distribution of vascular plants. We investigated two study sites from April to September, 2011. Total 477 taxa with 93 families, 279 genera, 408 species, 4 subspecies, 56 varieties and 9 forms were distributed in Mt. Gwaebyung and Galmi-Bong. Korean endemic species were composed of 8 families with 11 genera and 11 species. The plant species which is designated as protected species by the Ministry of Environment, Korea was 2 families, 2 genera and 2 species. Also plant species which is designated by the Korea National Arboretum was 16 taxa with 13 families, 14 genera, 15 species and 1 variety. A naturalized plants were 17 taxa. The Naturalization index and Urbanization index were 3.6% and 5.3% respectively. Pteridophyta-calculation (Pte-Q) was 1.00. Life form spectra was $H-D_4-R_5-e$ type and the highest percentage of useful plant resources were medicinal plant (77.5%). The forest vegetation in Mt. Gwaebyung and Galmi-bong was classified into 1 order, 1 alliance and 4 communities; Rhododendro - Quercetalia mongoliaceae, Lindero-Quercion mongolicae, Quercus mongolica Typical Community, Pinus densiflora-Quercus mongolica Community, Larix kaempferi Community and Sasa borealis-Quercus mongolica Community.

Effects of Replacing Mushroom By-product with Tofu By-product on the Chemical Composition, Microbes, and Rumen Fermentation Indices of Fermented Diets (두부비지의 버섯 폐배지 대체 수준이 발효사료의 영양소 함량, 미생물 성상 및 반추위 내 발효특성에 미치는 영향)

  • Joo, Young-Ho;Jeong, Hui-Han;Kim, Dong-Hyeon;Lee, Hyuk-Jun;Lee, Seong-Shin;Kim, Sang-Bum;Kim, Sam-Churl
    • Journal of Environmental Science International
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    • v.26 no.5
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    • pp.651-659
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    • 2017
  • This study aimed to estimate the effects of replacing Mushroom By-Product (MBP) with Tofu By-Product (TBP) on the chemical composition, microbes, and rumen fermentation indices of Fermented Diets (FDs). The basal diet was formulated using MBP, TBP, rice bran, molasses, and inoculants. The MBP in the basal diet was replaced with TBP at 0, 5, and 10% on Dry Matter (DM) basis for the experimental diets. The experimental diets were fermented at $39^{\circ}C$ for 144 h. Chemical composition, pH, microbes, and rumen fermentation indices of the FDs were analyzed. With increasing TBP replacement, crude protein content of FDs increased (L, P < 0.001), whereas crude ash content decreased (L, P = 0.002). Lactic acid bacteria and Bacillus subtilis contents in the TBP-replaced FDs were higher than those in the control (P < 0.05), whereas pH level and mold count were lower (P < 0.05). With increasing TBP replacement, in vitro rumen digestibility of DM (L, P = 0.053) and neutral detergent fiber (L, P = 0.024) increased, wheres rumen pH changed (P = 0.026) quadratically. Rumen total volatile fatty acid (L, P = 0.001) and iso-butyrate contents (Q, P = 0.003) increased with increasing TBP replacement. In conclusion, this study indicates that the replacement of MBP with TBP could improve the quality of FD.

Analysis for Aerodynamic Resistance of Chrysanthemum Canopy through Wind Tunnel Test (풍동실험을 통한 국화군락의 공기유동 저항 분석)

  • Yu, In-Ho;Yun, Nam-Kyu;Cho, Myeong-Whan;Lee, In-Bok
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.83-89
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    • 2008
  • A wind tunnel test was conducted at Protected Horticulture Experiment Station of National Horticultural Research Institute in Busan to find the aerodynamic resistance and quadratic resistance coefficient of chrysanthemum in greenhouse. The internal plants of the CFD model has been designed as a porous media because of the complexity of its physical shapes. Then the aerodynamic resistance value should be input for analyzing CFD model that crop is considered while the value varies by crops. In this study, the aerodynamic resistance value of chrysanthemum canopy was preliminarily found through wind tunnel test. The static pressure at windward increased as wind velocity and planting density increased. The static pressure at leeward decreased as wind velocity increased but was not significantly affected by planting density. The difference of static pressure between windward and leeward increased as wind velocity and planting density increased. The aerodynamic resistance value of chrysanthemum canopy was found to be 0.22 which will be used later as the input data of Fluent CFD model. When the planting distances were $9{\times}9\;cm$, $11{\times}11\;cm$, and $13{\times}13\;cm$, the quadratic resistance coefficients of porous media were found to be 2.22, 1.81, and 1.07, respectively. These values will be used later as the input data of CFX CFD model.

Effects of Supplemental Humic Substances on Egg Production and Quality in Laying Hens (Humic Substances의 급여가 산란계의 산란율과 난 특성에 미치는 영향)

  • Wang, Q.;Yoo, J.S.;Chen, Y.J.;Kim, H.J.;Cho, J.H.;Min, B.J.;Park, B.C.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.33 no.4
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    • pp.317-321
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    • 2006
  • The effects of dietary humic substances (HS) on egg Production and egg Quality were studied using 252(55-wk old) ISA brown laying hens. laying were divided into 21 groups of 12 hens each and seven groups (experimental units) were assigned to 1) CON (basal diet), 2) HS5 (basal diet 4- 5% humic substances) or 3) HS10 (basal diet +10% humic substances) in a completely randomized block design. Hens had free access to diets and water fur 6 wk. Egg Production and egg quality were monitored over the 6-wk Period. Results showed that 10% dietary HS decreased egg Production and yolk diameter (P<0.05) compared to CON. Egg weight and yolk cole. were improved (P<0.05) in HS10 compared to CON. Egg shell breaking strength was increased significantly (P<0.05) when hens were fed HS5 diet compared to the others. There were no effects of treatments on egg shell thickness, yolk index, albumen height and Haugh nit. The results suggest that the dietary supplementation of HS at 5% or 10% decreases egg Production, but HS at 5% can increase egg shell breaking strength. Hens fed 10% HS could increase egg weight and yolk color and decrease yolk diameter.

Molecular epidemiological analysis of viscerotropic velogenic Newcastle disease viruses

  • Lee, Youn-Jeong
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2005.11a
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    • pp.44-52
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    • 2005
  • The study, using sequence analysis and phylogenetic relationship of the fusion protein gene, divided the Korean epizootic isolates of Newcastle disease virus (NDV) into several lineages to determine the molecular epidemiology of the virus. A 695 base pair fragment was amplified by polymerase chain reaction between matrix protein gene and fusion protein gene of 30 Korean NDV isolates, which were isolated from field outbreaks of Newcastle disease between 1949 and 2002. All isolates showed the amino acid sequence 112 R-R-Q/R-K-R116 at the C-terminus of the F2 protein and phenylalanine (F) at the N-terminus of the F1 protein, residue 117. These amino acid sequences were identical to a known virulent motif. The region of the F gene between nucleotides 47 and 435 was compared by phylogenetic analysis. Based on nucleotide sequence, the Korean NDV isolates belonged to genotype III, V, VI and VII corresponding to isolates in 1949, 1982 to 1984, 1988 to 1997, and 1995 to 2002, respectively. These data showed that genotypes of five Korean Newcastle disease epizootics had replaced each other serially (III, V, VI and VII) in chronological order. Further, the five Korean Newcastle disease epizootics were closely related with the Necastle disease panzootics or Newcastle disease epizootics in other countries. Present study showed that the Korean genotype V isolated before 1984 was related with European Newcastle disease epizootics in the 1970s, whereas the Korean genotype VI and VII isolated after 1988 were more closely related with Far East Newcastle disease epizootics, especially Newcastle disease3 epizootics in Japan, Taiwan and China. Since 1988, the genotype VI and VII of Far East origin were dominant in South Korea. That might be due to the increased trade of agricultural products including poultry among Far East Asian countries.

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