• Molecules and Cells
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• v.39 no.4
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• pp.299-309
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• 2016

We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.2
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• pp.177-181
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• 2015

The subfornical organ (SFO) is one of circumventricular organs characterized by the lack of a normal blood brain barrier. The SFO neurons are exposed to circulating glutamate ($60{\sim}100{\mu}M$), which may cause excitotoxicity in the central nervous system. However, it remains unclear how SFO neurons are protected from excitotoxicity caused by circulating glutamate. In this study, we compared the glutamate-induced whole cell currents in SFO neurons to those in hippocampal CA1 neurons using the patch clamp technique in brain slice. Glutamate ($100{\mu}M$) induced an inward current in both SFO and hippocampal CA1 neurons. The density of glutamate-induced current in SFO neurons was significantly smaller than that in hippocampal CA1 neurons (0.55 vs. 2.07 pA/pF, p<0.05). To further identify the subtype of the glutamate receptors involved, the whole cell currents induced by selective agonists were then compared. The current densities induced by AMPA (0.45 pA/pF) and kainate (0.83 pA/pF), non-NMDA glutamate receptor agonists in SFO neurons were also smaller than those in hippocampal CA1 neurons (2.44 pA/pF for AMPA, p<0.05; 2.34 pA/pF for kainate, p< 0.05). However, the current density by NMDA in SFO neurons was not significantly different from that of hippocampal CA1 neurons (1.58 vs. 1.47 pA/pF, p>0.05). These results demonstrate that glutamate-mediated action through non-NMDA glutamate receptors in SFO neurons is smaller than that of hippocampal CA1 neurons, suggesting a possible protection mechanism from excitotoxicity by circulating glutamate in SFO neurons.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.9
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• pp.2285-2301
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• 2012

Peer-to-Peer (P2P) networks are becoming more and more popular in video content delivery services, such as Video on Demand (VoD). Scalability feature of P2P allows a higher number of simultaneous users at a given server load and bandwidth to use stream service. However, the quality of service (QoS) in these networks is difficult to be guaranteed because of the free-riding problem that nodes download the recourses while never uploading recourses, which degrades the performance of P2P VoD networks. In this paper, a distributed trust model is designed to reduce node's free-riding phenomenon in P2P VoD networks. In this model, the P2P network is abstracted to be a super node hierarchical structure to monitor the reputation of nodes. In order to calculate the reputation of nodes, the Hidden Markov Model (HMM) is introduced in this paper. Besides, a distinction algorithm is proposed to distinguish the free-riders and malicious nodes. The free-riders are the nodes which have a low frequency to free-ride. And the malicious nodes have a high frequency to free-ride. The distinction algorithm takes different measures to response to the request of these two kinds of free-riders. The simulation results demonstrate that this proposed trust model can improve QoS effectively in P2P VoD networks.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10653-10658
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• 2015

Background: A very high incidence of lung cancer is observed in Mizoram and Manipur, North East India. We conducted a population based case control study to establish associations of p53 codon 72 polymorphisms and interactions with environmental factors for this high incidence. Material and Methods: A total of 272 lung cancer cases and 544 controls matched for age (${\pm}5years$), sex and ethnicity were collected and p53 codon 72 polymorphism genotypes were analyzed using a polymerase chain based restriction fragment length polymorphism assay. We used conditional multiple logistic regression analysis to calculate adjusted odds ratios and 95% confidence intervals after adjusting for confounding factors. Results: p53 Pro/Pro genotype was significantly associated with increased risk of lung cancer in the study population (adjusted OR=2.14, CI=1.35-3.38, p=0.001). Interactions of the p53 Pro/Pro genotype with exposure to wood smoke (adjusted OR=3.60, CI=1.85-6.98, p<0.001) and cooking oil fumes (adjusted OR=3.27, CI=1.55-6.87, p=0.002), betel quid chewing (adjusted OR=3.85, CI=1.96-7.55, p<0.001), tobacco smoking (adjusted OR=4.42, CI=2.27-8.63, p<0.001) and alcohol consumption (adjusted OR=3.31, CI=1.10-10.03, p=0.034) were significant regarding the increased risk of lung cancer in the study population. Conclusions: The present study provided preliminary evidence that a p53 codon 72 polymorphism may effect lung cancer risk in the study population, interacting synergistically with environmental factors.

• Journal of Information Processing Systems
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• v.2 no.1
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• pp.6-12
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• 2006

Peer-to-Peer (P2P) networks are attracting considerable research interest because of their scalability and high performance relative to cost. One of the important services on a P2P network is the streaming service. However, because each node in the P2P network is autonomous, it is difficult to provide a stable streaming service on the network. Therefore, for a stable streaming service on the P2P network, a fault-tolerant scheme must be provided. In this paper, we propose two new node selection schemes, Playback Node First (PNF) and Playback Node first with Prefetching (PNF-P) that can be used for a service migration-based fault-tolerant streaming service. The proposed schemes exploit the fact that the failure probability of a node currently being served is lower than that of a node not being served. Simulation results show that the proposed schemes outperform traditional node selection schemes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.5393-5399
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• 2014

To examine the effects of a mycosporine-like amino acids (MAAs) mixture from microalgae, Chlamydomonas sp, on the anti-wrinkle activities, the expression levels of genes that are associated with skin aging, including type I procollagen, elastin and involucrin, were analyzed. Asterina 330+palythine (A+P) and shinorine+palythine (S+P) mixtures were purified from Chlamydomonas sp using the following steps: 80% methanolic extraction, column purification, and HPLC analysis. As a result of the MTT assay, A+P and S+P did not induce cellular cytotoxicity with up to 0.1 mg/mL of both MAAs. In addition, the treatment of UV-exposed fibroblasts with A+P (0.05 mg/mL) and S+P (0.01 mg/mL) increased the levels of the PCOLCE mRNAs by 2.7 and 3.6 fold compared to the control group, respectively, The levels of elastin gene expression were elevated 5.59 and 3.1 fold in the A+P and S+P treated (0.01 mg/mL) cells, respectively. In particular, at a concentration of 0.01 mg /mL, the A+P and S+P expression levels of Involucrin mRNAs were increased 3.5 and 2.5 fold in the UV-exposed cells compared to the control, respectively. In conclusion, the MAAs derived from Chlamydomonas sp can be utilized as functional cosmetic materials with anti-wrinkle effects on the skin.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.854-859
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• 2015

This experiment was performed to develop a model for nutrition ion concentration and EC in regard to change in pH from 4.0 to 8.0 in nutrient solution. The pH changes according to the variation of $HPO_4{^{-2}}$ and $H_2PO_4{^-}$ in the nutrient solution while variation of EC increased from pH 4.0 to 5.0, stabilized from pH 5.0 to 7.0 and increased again from pH 7.0 to 8.0. For the variance of major elements in the nutrient solution, K, Ca, N and P increased while pH was higher, especially the variables for K and P were increased largely. On the other hand, variables of Mg and S were stable. Based on analysis of the ion balance model of nutrient solution, the cation increased than anion over rising the variation of pH while balance point of ion moved from a-side to d-side. In addition, the imbalance increased while it moved away from the EC centerline as variance of pH increased. It was larger than effect of EC variance to correction values of equivalence ratios of K and Ca about variation of $HPO_4{^{-2}}$ and $H_2PO_4{^-}$ while as variance of pH increased, K decreased but Ca increased. These showed the result that variance of pH about correction values of equivalence ratios of K and Ca gave a second-degree polynomial model rating of 0.97. Through this research, it was identified the pH variable model about variance among pH, ion and EC according to gradient of phosphate.

• Polymer(Korea)
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• v.33 no.5
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• pp.441-445
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• 2009

In order to develop an intelligent delivery system for cosmetic active agents (e.g., arbutin, ascorbic acid, and adenosine), pH-responsive P(MAA-co-PEGMA) hydrogel particles having an average size of approx. $2{\mu}m$ and spherical shape were synthesized via dispersion photopolymerization. There was a drastic change in the swelling ratio of P (MAA-co-PEGMA) hydro gels at a pH 5, which is the $pK_a$ of the hydrogel. To determine the factors that have an effect on the loading of cosmetic active agents into the hydrogel, the loading efficiency of the active agents was investigated at various MAA and EG compositions of the hydrogel and the loading pH conditions. When the MAA contents in the hydrogel decreased, the loading efficiency of the active agents increased. In loading experiments with different pH conditions, the active agents showed higher loading efficiency into the hydrogel at the pH where the hydrogel was at the swollen state than at the pH where the electrostatic repulsion was minimized.

• Journal of Animal Science and Technology
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• v.58 no.6
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• pp.19.1-19.7
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• 2016

Background: Cowpea (Vigna unguiculata [L.]) is a legume cultivated throughout most tropical countries and is valued as food and feed for human and livestock respectively. The search for an improved cowpea variety has been on-going with the aim of improving traits such as grain yield, drought and pest resistance. But no information exist on the feeding value of these improved varieties. Phosphate (P) fertilizer application is recommended to augment grain yield in grain legumes but data on the effect of P fertilizer on haulm quality is limited. Results: Two separate experiments were conducted to determine the effect of P fertilizer dose on the nutritive value of haulms from different cowpea varieties (V). In experiment 1, effect of three P doses (30, 60 and 90 kg $P_2O_5/ha$) on in vitro gas production (IVGP) characteristics, concentrations of digestible organic matter (DOM), crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF) of haulms from five cowpea varieties (Zaayura-SARC 4-75, Songotra-IT97K-499-35, Hewale-IT93K-192-4, IT99K 573-1-1 and Asomdwe-IT94K-410-2) were investigated using the $3(P){\times}5(V)$ factorial treatment arrangements in a completely randomized design. In experiment 2, the effects of two P doses (30 and 90 kg $P_2O_5/ha$) and two varieties (Zaayura-SARC 4-75 and Hewale-IT93K-192-4) on the voluntary feed intake, live weight, haematology and carcass characteristics of Djallonke lambs were also assessed using a $2(P){\times}2(V)$ factorial treatment arrangement. The $V{\times}P$ interaction significantly affected CP, NDF and ADF with CP concentration increasing with increase in P doses in Zaayura-SARC 4-75 and Asomdwe-IT94K-410-2. Whilst an increase (P < 0.05) in NDF was observed in Songotra-IT97K-499-35and Asomdwe-IT94K-410-2 as P doses increased, the other V only increased from P dose 30 to 60 kg/ha and declined at P dose 90 kg/ha. The ADF decreased (P < 0.05) with increase in P dose for all V with the exception of Songotra-IT97K-499-35. There was a significant V effect on DOM with the highest reported in Zaayura-SARC 4-75 (43.44 %). Daily DM intake, carcass length and blood urea nitrogen of the lambs were significantly affected by the V x P interaction. There was a significant V effect on globulin and P effect on live weight at slaughter, dressed weight, chuck, leg, loin, rib and flank and liver and lungs. Conclusion: It can be concluded that nutrient concentrations of cowpea haulms were positively influenced by different P dose and varieties with favorable effects on growth, haematology and carcass composition of lambs. Varieties Zaayura-SARC 4-75 and Hewale-IT93K-192-4 at P dose at 90 kg/ha are recommended to enhance growth performance and carcass yield of Djallonke lambs.

• Journal of applied mathematics & informatics
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• v.30 no.5_6
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• pp.793-807
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• 2012

If G is any connected graph of order p; then the thorn graph $G_p^*$ with code ($n_1$, $n_2$, ${\cdots}$, $n_p$) is obtained by adding $n_i$ pendent vertices to each $i^{th}$ vertex of G. By treating the pendent edge of a thorn graph as $P_2$, $K_2$, $K_{1,1}$, $K_1{\circ}K_1$ or $P_1{\circ}K_1$, we generalize a thorn graph by replacing $P_2$ by $P_m$, $K_2$ by $K_m$, $K_{1,1}$ by $K_{m,n}$, $K_1{\circ}K_1$ by $K_m{\circ}K_1$ and $P_1{\circ}K_1$ by $P_m{\circ}K_1$ and their respective generalized thorn graphs are denoted by $G_P$, $G_K$, $G_B$, $G_{KK}$ and $G_{PK}$ respectively. Many chemical compounds can be treated as $G_P$, $G_K$, $G_B$, $G_{KK}$ and $G_{PK}$ of some graphs in graph theory. In this paper, we obtain the bounds of the wiener index for these generalization of thorn graphs.