• Title/Summary/Keyword: $T_o$ generation

Search Result 163, Processing Time 0.018 seconds

The increased GUS gene inactivation over generation in Arabidopsis transgenic lines (애기장대 형질전환 식물체의 세대경과에 따른 GUS유전자의 비활성화에 관한 연구)

  • Park, Soon-Ki
    • Journal of Life Science
    • /
    • v.12 no.1
    • /
    • pp.67-76
    • /
    • 2002
  • The effect of transgene inactivation in T2, T3 and F2 generations was analyzed in progeny seedlings which had been generated by Agrobacterium (LBA4404/pBI121)-mediated transformation in Arabidopsis thaliana. In a system which investigated in the expression of $\beta$-glucuronidase(GUS)gene in kanamycin-resistant (ke $n^{R}$)seedlings, GUS inactivated seedlings were observed in 5 of 12 tested lines of T2 generation and the frequency of GUS inactivation was approximately 2.3%. Lines with multi-copies of T-DNA exhibited severe GUS gene inactivation with the frequency of 5.8% in T2 generation. In T3 generation lines exhibited GUS gene inactivation with the frequency of 1.3%. In contrast, inactivation increased dramatically up to 12.6% in multi-copy T-DNA line. A similar phenomenon was also found in F2 progeny from a transgenic line which had been crossed with wild-type Arabidopsis plant, WS-O (GUS gene inactivation frequency 9.9%). These results indicate that the foreign gene introduced into the plant was inactivated progressively in its transmission during subsequent generations and the transgenic line with multi-copies of T-DNA tended to show more increased inactivation.

Temperature Dependent Characteristics of a Combined Discharge Type Ozonizer (CDO)

  • Fayzur Rahman;B. J. Chun;Lee, K. S.;Lee, D. I.
    • KIEE International Transactions on Electrophysics and Applications
    • /
    • v.3C no.3
    • /
    • pp.106-112
    • /
    • 2003
  • A combined discharge type ozonizer was designed and manufactured. The increase or decrease of temperature greatly influences the characteristics of ozone concentration ( $O_{3con}$), ozone generation ( $O_{3g}$) and ozone yield ( $O_{3Y}$) of a discharge type ozonizer. The characteristics of ozone concentration, ozone generation and ozone yield rate were investigated by varying the gas flow rate (Q), the discharge power ( $W_{d}$) and the temperature (T). At T=25[$^{\circ}C$] the values of $O_{3con}$ were found as 5632, 4200, 2500 and l800[ppm] at Q = 1, 2, 4 and 6[l/min] respectively. At the same temperature the corresponding values of $O_{3g}$ were found as 662, 988, 1176 and l270[mg/h] and those of $O_{3Y}$ were found as 67, 102, 119 and 135[g/kWh] respectively. When the temperature is decreased to -50[$^{\circ}C$], the values of $O_{3con}$ were found as 9000, 6700, 4000 and 2800[ppm] respectively at Q = 1, 2, 4 and 6[l/min]. At the same value of temperature the corresponding values of $O_{3g}$ were found as 1220, 1576, 1882 and 2050[mg/h] and those of $O_{3Y}$ were found as 120, 159, 188 and 202[g/kWh] respectively. Hence as the temperature was decreased from 25 to -50[$^{\circ}C$], the efficiencies of ozone generation were increased by 79, 55, 58 and 49[%] respectively at Q = 1, 2, 4 and 6[l/min].]. 6[l/min].].

Study of SiO2 Thin Film Patterning by Low Energy Electron Beam Lithography Using Microcolumns (저 에너지 초소형 전자칼럼 리소그래피를 이용한 SiO2 박막의 Pattern 제작에 관한 연구)

  • Yoshimoto, T.;Kim, H.S.;Kim, D.W.;Ahn, S.
    • Journal of the Korean Magnetics Society
    • /
    • v.17 no.4
    • /
    • pp.178-181
    • /
    • 2007
  • Electron beam lithography has been studied as a next-generation lithography technology instead of photo lithography for ULSI semiconductor devices. In this work, we have made a low-energy electron beam lithography system based on the microcolumn and investigated the dependence of the pattern thickness on the energies and dose concentration of the electron beam. We have also demonstrated the potential of low-energy lithography by achieving 100 nm-$SiO_2$ thin film patterning.

Effect of Temperature on the Performance Characteristics of a Pin-Cylinder Discharge Type Ozonizer

  • Md. Fayzur Rahman;Chun, Byung-Joon;Lee, Kwang-Sik;Lee, Dong-In
    • KIEE International Transactions on Electrophysics and Applications
    • /
    • v.12C no.4
    • /
    • pp.201-207
    • /
    • 2002
  • A Pin-Cylinder discharge type ozonizer was designed and manufactured. The increase or decrease of temperature greatly influences on the characteristics of ozone generation of a discharge type ozonizer. The characteristics of ozone concentration ( $O_{3con}$), ozone generation ($_{3g}$) and ozone yield .ate ($_{3Y}$) of the ozonizer were investigated by varying the gas flow rate (Q), the discharge power ($_{Wd}$ ) and the temperature (T). At T = 20($^{\circ}C$), the values of ( $O_{3con}$) were found as 7800,5300,3000 and 2300(ppm) at Q=1,2,4 and 6(1/min) respectively. The corresponding values of ( $O_{3g}$) were found as 917, l247,1411 and 1623(mg/h) and those of ( $O_{3Y}$) were 93,126,143 and 164(g/kWh) respectively. When the temperature is decreased to -50($^{\circ}C$), the values of ( $O_{3con}$) became 12000,8000,5200 and 3600(ppm) at Q=1,2,4 and 6(1/min) respectively. The corresponding values of 0,9 were obtained as 1411,1882,2446 and 2600(mg/h) and those of ($O_{3Y}$) were 143,190,247 and 2631g/kWh] respectively. Hence as the temperature was decreased from 20 to -50[。C], the efficiencies of ozone generation were increased by 54,51,73 and 60[%] at Q=1,2,4 and 6(1/min) respectively.ctively.

A Surface Generation Velocity Measurement Technique Using the Buried Channel MOS Structure (Buried Channel MOS 구조를 이용한 표면생성속도 측정 방법)

  • 조성호;허연철;이종덕
    • Journal of the Korean Institute of Telematics and Electronics A
    • /
    • v.29A no.7
    • /
    • pp.56-63
    • /
    • 1992
  • A measurement technique of the surface generation velocity S$_o$ using the BC(buried channel) MOS structure with shallow and low doped channel layer (BC MOS S$_o$ measurement technique) is presented and verified analytically and experimentally. Using this measurement technique, S$_o$ can be measured more accurately than that measured using the gate-controlled diode SS1oT measurement technique. When S$_o$ is measured for the two techniques from a BC MOS structure test patten with gate length of 171$\mu$m, the results are 0,66cm/sec and 0.28cm/sec for the former and the latter respectively.

  • PDF

Studies of the Smoke Emission from Cellulose Fiber Insulation (셀룰로오스 단열재의 연기발생에 관한 연구)

  • Kim, Hong;Kang, Young-Goo
    • Journal of the Korean Society of Safety
    • /
    • v.9 no.2
    • /
    • pp.12-17
    • /
    • 1994
  • Smoke evolved from burning cellulose insulation treated with boric acid-borax-alum as combustion retardants has been studied using a light absorption method. It has been shown that the quantity of smoke are decreased with the increase of oxygen concentration. Applied fire retardants did not increase smoke generation from investigated cellulose insulation in range of themal degradation without flaming. These same fire retardants increased from in the range of flaming combustion. The results of this work have been analysed using imperical equation, which correlated the smoke density at oxygen content of combustion transition. This equation may be. $\sigma$=$\sigma$$_{T}$ exp{-7.24$\times$10$^{-7}$ ( $O_2$ $O_{2.T}$)}Where $O_2$is the concentration of oxygen in the oxidizing gas, and $O_{2.T}$ refers to oxygen concentration of combustion transition.n.n.n.

  • PDF

4'-O-β-D-Glucosyl-5-O-Methylvisamminol Attenuates Pro-Inflammatory Responses and Protects against Oxidative Damages

  • Yoo, Ok-Kyung;Keum, Young-Sam
    • Biomolecules & Therapeutics
    • /
    • v.27 no.4
    • /
    • pp.381-385
    • /
    • 2019
  • We attempted to examine anti-inflammatory and anti-oxidant effects of 4'-O-${\beta}$-D-glucosyl-5-O-methylvisamminol (GOMV), the first epigenetic inhibitor of histone phosphorylation at Ser10. While GOMV did not affect the viability of murine macrophage RAW 264.7 cells, it significantly suppressed lipopolysaccharide (LPS)-induced generation of prostaglandin $E_2$ ($PGE_2$) and nitric oxide (NO) through transcriptional inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). GOMV also scavenged free radicals in vitro, increased NF-E2-related factor 2 (NRF2), and activated antioxidant response element (ARE), thereby resulting in the induction of phase II cytoprotective enzymes in human keratinocyte HaCaT cells. Finally, GOMV significantly protected HaCaT cells against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative intracellular damages. Together, our results illustrate that GOMV possesses anti-inflammatory and anti-oxidant activity.

The Design of Oxide Module for High Temperature Thermoelectric Power Generation (고온 발전용 산화물 열전모듈의 설계)

  • Park, Jong-Won;Yoon, Sun-Ho;Cha, Jeong-Eui;Choi, Seung-Chul
    • Journal of the Microelectronics and Packaging Society
    • /
    • v.15 no.4
    • /
    • pp.93-100
    • /
    • 2008
  • The one and two pair of oxide modules for high temperature thermoelectric power generation were fabricated with $Ca_{2.7}Bi_{0.3}Co_4O_9$(p-type) and $Ca_{0.96}Bi_{0.04}Mn_{0.96}Nb_{0.04}O_3$(n-type) on $Al_2O_3$ substrate. For the optimizing of the design process, contact resistance was derived from the results of the one pair modules, and then the resistance of two pair modules were calculated to use the derived data. Those values were compared with the measured values for the optimizing of this design process. The resistance of calculated and measured two pairs modules was 0.956 $\Omega$ and 1.110 Q $\Omega$ $T_h$=833 K, respectively, the difference of resistance was about 0.15 $\Omega$. From the result, proposed design process is effective for high temperature thermoelectric oxide modules fabrication.

  • PDF

Preparation and Nonlinear Optical Properties of Novel Polyesters with Enhanced Thermal Stability of Second Harmonic Generation

  • Kim, Jin-Hyang;Won, Dong-Seon;Lee, Ju-Yeon
    • Bulletin of the Korean Chemical Society
    • /
    • v.29 no.1
    • /
    • pp.181-186
    • /
    • 2008
  • 2,5-Di-(2'-hydroxyethoxy)-4'-nitrostilbene (3) was prepared and polycondensed with terephthaloyl chloride, adipoyl chloride, and sebacoyl chloride to yield novel T-type polyesters (4-6) containing the NLO-chromophores dioxynitrostilbenyl groups, which constituted parts of the polymer backbones. Polymers 4-6 are soluble in common organic solvents such as acetone and N,N-dimethylformamide. They showed thermal stability up to 260 oC in thermogravimetric analysis with glass-transition temperatures obtained from differential scanning calorimetry in the range 90-95 oC. The second harmonic generation (SHG) coefficients (d33) of poled polymer films at the 1064 nm fundamental wavelength were around 1.42 ´ 10-9 esu. The dipole alignment exhibited high thermal stability up to 5 oC higher than glass-transition temperature (Tg), and there was no SHG decay below 100 oC due to the partial main-chain character of polymer structure, which is acceptable for NLO device applications.

Thermal Behavior of NiFe2O4 for Hydrogen Generation (NiFe2O4를 이용한 열화학 사이클 H2 제조)

  • Han, S.B.;Kang, T.B.;Joo, O.S.;Jung, K.D.
    • Transactions of the Korean hydrogen and new energy society
    • /
    • v.14 no.4
    • /
    • pp.298-304
    • /
    • 2003
  • The thermal behavior of $NiFe_2O_4$ prepared by a solid-state reaction was investigated for $H_2$ generation by the thermochemical cycle. The reduction of $NiFe_2O_4$ started from $800^{\circ}C$, and the weight loss was 0.2-0.3 wt% up to $1000^{\circ}C$. In the $H_2O$ decomposition reaction, $H_2$ was generated by oxidation of reduced $NiFe_2O_4$. The crystal structure of $NiFe_2O_4$ maintained during the redox reaction of 5 cycles. From this observation, the lattice oxygen in $NiFe_2O_4$ is released without the structural change during the thermal reduction and oxygen deficient $NiFe_2O_4$ can be restored to the spinel structure of $NiFe_2O_4$.