• Molecules and Cells
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• 제26권3호
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• pp.265-269
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• 2008

Calumenin, a multiple EF-hand $Ca^{2+}$ binding protein is located in the SR of mammalian heart, but the functional role of the protein in the heart is unknown. In the present study, an adenovirus gene transfer system was employed for neonatal rat heart to examine the effects of calumenin over-expression (Calu-OE) on $Ca^{2+}$ transients. Calu-OE (8 folds) did not alter the expression levels of DHPR, RyR2, NCX, SERCA2, CSQ and PLN. However, Calu-OE affected several parameters of $Ca^{2+}$ transients. Among them, prolongation of time to 50% baseline ($T_{50}$) was the most outstanding change in electrically-evoked $Ca^{2+}$ transients. The higher $T_{50}$ was due to an inhibition of SERCA2-mediated $Ca^{2+}$ uptake into SR, as tested by oxalate-supported $Ca^{2+}$ uptake. Furthermore, co-IP study showed a direct interaction between calumenin and SERCA2. Taken together, calumenin in the cardiac SR may play an important role in the regulation of $Ca^{2+}$ uptake during the EC coupling process.

• 대한가정학회지
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• 제50권1호
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• pp.51-63
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• 2012

The purpose of this study was to investigate the effect of appearance management cognition on attitude toward appearance management and the effect of this attitude on appearance management behavior. It also examines how demographic factors affect these relationships. This study gave a questionnaire survey to adults between the ages of 40 and 50 in Seoul, Daegu, and Ulsan, South Korea. Data collected from 368 respondents were analyzed using descriptive statistics, t-test, factor analysis, and structural equation modeling. The study model was tested by structural equation modeling, the results of which revealed a positive effect of appearance management cognition on attitude toward appearance management and a positive effect of attitude toward appearance management on appearance management behavior. The results of t-testing showed that there were significant differences in cognition, attitude, and behavior toward appearance management by gender, age, educational level, and income.

• 약학회지
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• 제45권3호
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• pp.310-319
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• 2001

To compare skin irritation and cytotoxicity of anti-wrinkle agents, we examined skin irritation of six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB) in New Zealand white rabbit. Cytotoxicity of these agents was determined by MTT [tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] at multi-time points in cultured HaCaT cell, a human immortalized keratinocyte cell. We then analyzed correlation between skin irritation and cytotoxicity by spearman's rank correlation analysis. All trans-retinoic acid showed the highest primary irritation index (0.92) in skin irritation test. Being all the six agents not irritant, retinal showed the most cytotoxic agents. The correlation between skin irritation and cytotoxicity ($IC_{50}$/ at different time point was 0.814, 0.757, 0.814 and 0.7 at 3, 24, 48 and 72 h, respectively. We also fecund that IC$_{20}$ and IC$_{80}$ of these agents showed similar correlation with skin irritation. These results therefore demonstrated that there is close correlation between skin irritation and cytotoxicity $IC_{50}$/ value by MTT in HaCaT cell at early time points by anti-wrinkle agents or IC$_{20}$ value. $IC_{50}$/ at earily time point or IC$_{20}$ values may be reliable alternative determinant of skin irritation.n.

• Archives of Pharmacal Research
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• 제18권6호
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• pp.396-401
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• 1995

As a part of trials to develop the antitumor agent from tannins isolated from plants, the antitumor activity of peduculagin, an ellagitannin, isolated from Alnus hirsuta var. microphylla was examined in vitro and in vivo. In vitro, the cytotoxicity was determined by 0.4% typanblue dye exclusion method. peduculagin showed the dose-dependent cytotoxicity against human chronic myelogenous leukemia (K-562), human promyelocytic leukemia (HL-60), mouse lymphoid neoplasm (P388), mouse lymphocytic leukemia (L1210) and mouse sarcoma 180(S180) cell lines. $ED_{50}\; values\; (ED_{50})$ of each cell line were 5.30, 0.92, 2.78, 9.35 and $1.38 \mug/ml$ respectively. The most sensitive cell line was HL-60. In vivo, pedunculagin was administered to ICR mouse with the doses of 50 and $100{\;}{\mu}g/ml$intraperitoneally once at 20 days before S180 inoculation. peduculagin showed the antitumor activity and its T/C ratio (%) was 120.82% in the group of both concentrations.

• 한국환경과학회지
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• 제32권2호
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• pp.139-143
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• 2023

The effects of essential oils on pH, pathogens, and volatile fatty acids (VFA) in two poultry litters were investigated through a lab study. Essential oil-added poultry litters were randomly divided to two groups: control (200 g poultry litter) and Treatment (50 g thymol/Briefly, 200 g broiler litter was treated with or without 50 g thymol (Control and T1, respectively; 1 groups) and 200 g duck litter was treated with or without 50 g carvacrol (Control and T2, respectively; 2 group). Adding thymol to broiler litter increased the pH, reduced pathogens, and did not affect VFA. Interestingly, adding carvacrol slightly reduced the pH of duck litter, but had no significant effect on reducing pathogens and VFA. This difference is probably because the essential oil used and the properties of the two litters are different. In addition, pH was thought to control the odor generated from the litter, but this has not been proven. Further field studies should focus on clarifying this point.

• Parasites, Hosts and Diseases
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• 제54권2호
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• pp.187-190
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• 2016

Trichomoniasis is the most common curable sexually-transmitted infection (STI) worldwide. There are few reports on the prevalence of Trichomonas vaginalis in Korea. The purpose of this study was to examine the prevalence of trichomoniasis by PCR in Guri city, Korea. All adult women who visited Hanyang University Guri Hospital for health screening within the National Health Care Service were invited to participate in the study, and 424 women were enrolled between March and June 2011. PCR was used to detect Trichomonas vaginalis using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Fourteen women (3.3%) were found to have T. vaginalis. All were over 50, and they were significantly older on average than the 410 Trichomonas-negative women (mean ages 63.4 vs 55.3 years). It seems that T. vaginalis infection is not rare in women receiving health screening, especially among those over 50.

• BMB Reports
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• 제50권12호
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• pp.601-609
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• 2017

Mammalian target of rapamycin complex 1 (mTORC1) plays a major role in cell growth, proliferation, polarity, differentiation, development, and controls transitioning between anabolic and catabolic states of the cell. It collects almost all extracellular and intracellular signals from growth factors, nutrients, and maintains cellular homeostasis, and is involved in several pathological conditions including, neurodegeneration, Type 2 diabetes (T2D), obesity, and cancer. In this review, we summarize current knowledge of upstream signaling of mTORC1 to explain etiology of T2D and hypertriglyceridemia, in which state, the role of telomere attrition is explained. We discuss if chronic inhibition of mTORC1 can reverse adverse effects resulting from hyperactivation. In conclusion, we suggest the regulatory roles of telomerase (TERT) and hexokinase II (HKII) on mTORC1 as possible remedies to treat hyperactivation. The former inhibits mTORC1 under nutrientrich while the latter under starved condition. We provide an idea of TOS (TOR signaling) motifs that can be used for regulation of mTORC1.

• 한국식품조리과학회지
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• 제18권6호
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• pp.684-691
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• 2002

The objectives of this study were to determine the consumption pattern and importance of Kimchi in our meals, and the preference of Kimchi. The 77.0% of the housewife respondents were preparing Kimchi at home. The reasons for not making Kimchi were ‘don't have time’(39.4%), ‘don't know how to make Kimchi’(18.2%) and ‘don't eat much’(18.2%). The amounts of daily Kimchi intake of the respondents were ‘less than 50g’(42.3%) or ‘50-100g’(29.8%). They are Kimchi because it was ‘delicious’(48.4%) or ‘habitual’(39.5%). The 70.0% of the respondents answered that Kimchi was important in each meal. The most respondents favored ‘moderate’ in the degree of fermentation(48.1%), hot taste(65.0%) and salty taste(72.4%). As the age of the respondents increased, they favored less sour, less hot and less salty taste. Baechu Kimchi(4.15), Chonggak Kimchi(3.89), Dongchimi(3.85), Kkaktugi(3.74), water Kimchi(3.67) were preferred by the respondents. The 73.97% of the respondents preferred Kimchi Jjigye. The 10- to 19-years old respondents preferred Kimchi Jjigye(52.8%), Kimchi pan cake(24.5%) and fried rice with Kimchi(17.0%).

• International Journal of Precision Engineering and Manufacturing
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• 제2권3호
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• pp.5-10
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• 2001

Due to the development of high-strength fibers and adhesives, it is now possible to repair cracked metallic plates by bonding reinforced patches to the plate over the crack. In this study, pre-cracked aluminum 6061-T6 alloy plates repaired with bonded carbon/epoxy composite patch are applied to investigate the effect of various patch shapes on the tensile strength and the fatigue behavior of the structure. A non-patch-boned cased and 2 type-50$\times$50, 40$\times$20 mm-composite patch-bonded cases were tested to obtain fracture loads and fatigue crack growth rate. The results showed that the patch-bonded repair improves the static strength by 17% and the fatigue life by 200% compared to non-repaired case. It means that patch-boned repair is more effective in the fatigue life. It was also revealed that the patching method along crack growth direction is more efficient in cost and weight reduction. By observing the fractography, patch-bonded repair specimens demonstrated zigzag fracture patterns compared with the non-patched specimens, which shows a typical ductile fracture.

• 아시안잔디학회지
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• 제10권2호
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• pp.167-175
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• 1996

This study was carried out to investigate the removal rate of sulphur of the litters in the Phragmites communsis, Seirpus tabernaemontani, Miscanthus sacchariflorus and Typha angustata aquatic grassland ecosystem on the lake Paldangho. The annual litter productions of sulphur were 50.91 /$m^2$, in P. communis, 180.83 g /$m^2$, in S. tabernaemontani, 25.87 g /$m^2$, in M. sacchariflorus and 151.39 g /$m^2$, in T. angustata, respectively. The removal rates r, of sulphur in the litters were 0.86 in P. communis, 0.82 in S. tabernaemontani, 0.43 in M. sacchariflorus and 0.47 in T. angustata respectively, The times required to reach 50, 95 and 99 percent of the steady state levels and turnover values of sulphur on the grass-land floor were 0.81, 3.49 and 5.82 years in the P. communis, 0.85, 3.68 and 6.13 years in the S. tabernaemontani, 1.62, 7.00 and 11.67 years in the M. sacchariflorus and 1.49, 6.44 and 10.73 years in the T. angustata, It is considered that the high removal rates of sulphur in four grasslands of aquatic ecosystem contribute to the efficient removal of sulphur, a pollutant, at the lake Paldangho. Key words: Removal rate, Accmulation, Paldaugho, Sulphur, Phragmites communis, Miscanthus saechariflorus, Seirpus tabernaemontani, Typha angastata.