• 미생물학회지
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• 제8권2호
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• pp.69-76
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• 1970

1. The yeasts in the two samples of Nuruk (mold wheat) which one prepared at the College of Agriculture, Choong-Nam University (S) and the other purchased at a market(T), were examined and counted. The yeasts were differe entiated by the market (T), were examined and counted. The yeasts were differe entiated by the TTC(2,3,5-triphenyltetrazolium chloride)agar overlay technique that yields a varied shade of color. The results were : the population of yeasts in 1g of Nuruk S was about $6{\times}10^4$, 56.5% of which were TTC-pink yeasts, 16% TTC-red pink yeasts, 8% were TTC-red yeasts, and 16.5% TTC-white yeasts. In Nuruk T(1g), the number of yeasts accounted for $14{\times}10^4$ and constituted of 42% TTC0pink, 21% TTC-red pink, 23% TTC-red and 9% TTC-white. 2. During the fermentation of Takju (Korean Sake) employing the Nuruk S and T the yeast flora throughout the brewing were observed in 12 hour intervals. TTC pink and red yeasts considered to be the major yeasts were isolated all cultured. The strains ($1{\times}10^5$/ml) were added to the mashes S and T in which pH was adjusted to 4.2 and the change of yeast flora was examined during fermentation.

• 대한환경공학회지
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• 제37권2호
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• pp.73-80
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• 2015

본 연구에서는 잔류의약물질로써 환경에서 노출 빈도가 높은 항생물질인 Tetracycline (TTC)을 대상으로 수용액 상에서의 망간산화물(birnessite)을 이용한 산화-변환 반응을 통한 제거와 용존성 자연유기물인 휴믹산(HA) 존재에 따른 영향을 조사하였다. TTC의 산화-변환 제거 특성 실험은 다양한 반응조건(반응시간, 망간산화물 주입량 및 pH 등)에서 회분식으로 조사하였으며, HA의 영향은 pH (3~9)와 HA농도 변화에 따라 조사하였다. 실험결과는 유사-일차 반응속도식을 적용하여 해석하였으며, 본 실험조건(TTC=0.25 mM, ${\delta}-MnO_2=1.0g/L$)에서의 TTC의 산화-변환 제거 반응속도상수(k, $hr^{-1}$)는 pH가 감소함에 따라 0.98 (pH 9)에서 2.97 (pH 3)로 일정하게 증가하였고, 망간산화물 주입량(1~2 g/L)이 2배 증가할 때 반응속도 상수는 약 1.3배 정도 증가하였다. HA (5 mg-C/L) 존재 시 망간산화물에 의한 TTC 변환제거는 $pH{\geq}6$에서 반응효율 상승을 나타냈으며, HA의 주입농도(1~10 mg-C/L, at pH 6)의 증가에 따라 일정하게 증가함을 확인하였다. 이상에서 얻은 연구결과는 기존 문헌에 제시된 TTC 반응기작과 비교 해석하였고, 망간산화물을 매개로 한 TTC의 산환-변환 제거 반응특성과 HA의 존재가 미치는 영향에 대하여 고찰하였다.

• 한국동물위생학회지
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• 제15권2호
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• pp.101-108
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• 1992

This study was carried out to investigate the effect of sulfamethazine and trimethoprim on TTC test in raw milk. Ten raw milks which does not contain any antibiotics were collected from milk farms and one hundred twenty raw milks which collected from three raw milk receiving stations and those milk samples were mixed up sulfamethazine and trimethoprim for TTC test. The results were as follows ; 1. Positive reactions of TTC test were shown above the concentration of 3, 000ppm for sulfamethazine and 10ppm for trimethoprim. 2. When the TTC test was conducted with trimethoprim and sulfamethazine mixture, a pair of the concentration of trimethoprim and sulfamethazine shown to positive reaction were 0.1-2, 000ppm, 0.5-1, 000ppm, 1.0-250ppm and 2.0-100ppm, respectively. 3. Of 120 raw milk samples tested, 16 samples(13%) were shown quasi-positive reaction when the trimethoprim at the concetration of 2ppm was added in the samples.

• 한국동물위생학회지
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• 제22권1호
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• pp.93-101
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• 1999

This test process on screening method for the detection of residual antibiotics in milk is simple, economic, sensitive to residual antibiotics and was given approval international organs. Thus, this study was carried out the comparison of Disk assay method and TTC-II method for sensitivity and minimum detectable range of antibiotics in raw milk. The results of this study was summarized as follows ; 1. The number of samples requested for treatment of mastitis was 198 samples. Comparison or analytical results among the methods of TTC-II, disk assay and Delve sp was that TTC-II 37 samples(18.6% ), Disk assay 125samples(63.1%), Delve SP 130 samples(65.7% ) reacted positively. Conformity rate of Delve SP and Disk assay was 70%. 2. Detectable limits of disk assay method in some antibiotics were more sensitive than those of official method(0.05-0.0025ppm in the $\beta$-lactams, 1ppm in two aminoglycoside, 0.2 ppm in one tetracycline, similar in one macrolide) 3. For sensitivity of residual sulfonamides TTC-II was much more sensitive than disk assay. Detectable limits of sulfamethazine and sulfadimethoxine were 30 to 50ppm levels. 4. The best medium preservation period is 1-2 days. 5. Concentration of brome cresol purple related to resistance for B stearothermophilus culture was 24ppm/ml. These results show that disk assay method for screening detection of antibiotics residuces in milk is worthy of use.

• 한국동물위생학회지
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• 제23권4호
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• pp.335-340
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• 2000

The study was Investigated detectable limits of antibiotic drugs in treatment of mastitis by TTC II reduction test. The detectable limits of antibiotic drugs by TTC II reduction test were summarized as follows, 1. Detectable limits to compounds drugs were higher than those of monodrugs. 2. Detectable limits to ointments were lower than injections.

• 대한핵의학회지
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• 제30권4호
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• pp.484-492
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• 1996

허혈성 뇌 병변에서 $^{99m}Tc$-glucarate의 섭취에 관한 연구를 하기 위하여 중뇌동맥 폐쇄 쥐 뇌허혈 모델을 재관류한 군과 하지 않은 군으로 나누어 만들었다. $^{99m}Tc$-glucarate와 [$^{18}F$]FDG를 연속적으로 투여하여 그 분포 양상을 이중 자가방사촬영법으로 관찰하였다. 조직의 괴사 여부를 알기 위하여 TTC 염색도 실시하여 동일한 뇌표본에 대하여 3가지의 영상을 동시에 얻을 수가 있었다. 얻은 영상으로 섭취 또는 비섭취정도를 보아 0점에서 3점까지 점수를 매겨 합하여 비교하였다. 수술한 쥐들 18마리 중 10마리가 신경학적 증상을 보이면서 살아남아 실험대상이 되었다. TTC 염색으로 확인한 경색크기는 재관류하지 않은 군이 컸다. [$^{18}F$]FDG 섭취양상은 TTC 염색과 거의 비슷하였다. 다만 일부에서 TTC 염색되는 곳에 [$^{18}F$]FDG 가 중간정도로 섭취되는 곳이 있었고 TTC 염색되지 않는 곳에 [$^{18}F$]FDG가 중간정도 섭취되는 곳이 있었다. TTC로 염색된 부위에는 $^{99m}Tc$-glucarate가 섭취되지 않았다. TTC로 염색되지 않는 곳에 일부분 $^{99m}Tc$-glucarate가 섭취되었다. TTC와 [$^{18}F$]FDG가 염색되거나 섭취되지 않는 곳에 $^{99m}Tc$-glucarate가 섭취되지 않는 곳이 있었다. 그러나 [$^{18}F$]FDG의 중간 정도의 섭취나 [$^{18}F$]FDG와 TTC사이에 부합하지 않는 곳 등과 $^{99m}Tc$-glucarate 섭취와 대응시키기 어려웠다 $^{99m}Tc$-glucarate가 재관류 군에서 더 넓고 많이 섭취되었다. 결론적으로 $^{99m}Tc$-glucarate는 비생존 허혈조직에만 섭취되는데 관류재개통에 따라 다양하게 섭취되었고 재관류모델에 더 많이 넓게 섭취되었다. 중뇌동맥폐색 및 재관류 모델로 $^{99m}Tc,\;^{18}F$ 및 TTC 동시영상을 얻어 생존능과 포도당대사의 부합 비부합 여부를 밝히고 그 의의를 조사한 후 $^{99m}Tc$-glucarate섭취와 상관을 조사하면 $^{99m}Tc$-glucarate섭취의 의의를 밝힐 수 있을 것으로 본다.

• Molecules and Cells
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• 제24권1호
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• pp.27-36
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• 2007

The hypothetical protein TTC0263 of Thermus thermophilus HB27 is a thermophilic tetratricopeptide repeat (TPR)-containing protein. In the present study, the TPR region (residues 26-230) was resolved at $2.5{\AA}$ with R-factors of $R/R_{free}$ = 23.6%/28.6% $R/R_{free}=23.6%/28.6%$. TTC0263 consists of 11 helices that form five TPR units. Uniquely, it contains one atypical "extended" TPR (eTPR) unit. This comprises extended helical residues near the loop region of TTC0263, such that the helical length of eTPR is longer than that of the canonical TPR sequence. In addition, the hybrid TPR domain of TTC0263 possesses oligomer-forming characteristics. TPR domains are generally involved in forming multi-subunit complexes by interacting with each other or with other subunit proteins. The dynamic structure of TTC0263 described here goes some way to explaining how TPR domains mediate the formation of multi-subunit complexes.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 1998년도 추계종합학술대회 논문집
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• pp.155-158
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• 1998

The KOMPSAT system incorporates multiple missions designed to provide various applications in the field of Korean peninsula observation covering land, sea and coastal zones. The missions are Korea cartography (1:25,000 scale maps of the Korean peninsula), biological oceanography and science instrument accommodation. The operational availability of KOMPSAT TTC system should be predicted more accurately because its failure has a significant influence on satellite command and tracking and on satellite data collection. In this paper, system availability structure of KOMPSAT TTC system are made and availability of KOMPSAT TTC system is analyzed aspect to the system operation in accordance with logistic condition.

• 아시안잔디학회지
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• 제3권1호
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• pp.13-15
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• 1989

In oder to find out the effective seed germination testing method to the field emergence, an experiment was conducted ; 1. TTC testing results were higher percentage than any other germination testing methods . 2. On the corn seed , field emergence was highly correlated with germinator test, TTC test and AA test hut cold test was lower percentage . 3. Field emergence , on the soybean seeds was highly correlated with AA test and cold test but germinator test and TTC test was higher percentage .

• 대한화장품학회지
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• 제40권3호
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• pp.307-311
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• 2014

2,3,5-Triphenyltetrazolium chloride (TTC)는 산화환원지시약으로 미생물의 증식에 의한 산소 소비를 쉽게 확인할 수 있다. 용해 후 무색의 형태를 띠고 있으나 생리활성이 있는 조직에서는 탈수소 효소(dehydrogenases)에 의해 환원되어 빨간색의 불용성 1,3,5,-triphenylformazan (TPF)가 된다. 본 연구에서는 병원성 미생물(Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecium, Candida albicans)에 TTC 지시약을 활용하여 미생물 성장시험에 대해 확인하였다. 시험 균주에 TTC를 첨가하여 확인한 결과, 모두 탈수소효소 반응으로 인한 TPF 형성으로 붉은색 콜로니를 관찰하였다. 이후 TTC 0.04% 이상의 농도 및 12 h 이상 배양조건으로 최적화 실험 후 균주별 CFU 값을 통해 TPF 발현능을 확인하였다. 결국 TTC가 병원성 세균 및 효모균 성장에 큰 영향을 끼치지 않으며 배양 시 세균의 경우 12 h, 효모균의 경우 48 h 이후부터 확인이 가능하였다. 이러한 결과들로부터 TTC를 활용한 미생물 성장 확인 시험법이 더 신속 정확한 방법으로 화장품 연구에 활용 가능할 것으로 사료된다.