• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1555-1562
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• 2010

Development of the strain and the fermentation process of Hansenula polymorpha was implemented for the production of ${\gamma}$-linolenic acid ($GLA,\;C18:3{\Delta}^{6,9,12}$), an n-6 polyunsaturated fatty acid (PUFA) that has been reported to possess a number of health benefits. The mutated ${\Delta}^6$-desaturase (S213A) gene of Mucor rouxii was expressed in H. polymorpha under the control of the methanol oxidase (MOX) promoter. Without the utilization of methanol, a high-cell-density culture of the yeast recombinant carrying the ${\Delta}^6$-desaturase gene was then achieved by fed-batch fermentation under glycerol-limited conditions. As a result, high levels of the ${\Delta}^6$-desaturated products, octadecadienoic acid ($C18:2{\Delta}^{6,9}$), GLA, and stearidonic acid ($C18:4{\Delta}^{6,9,12,15}$), were accumulated under the derepression conditions. The GLA production was also optimized by adjusting the specific growth rate. The results show that the specific growth rate affected both the lipid content and the fatty acid composition of the GLA-producing recombinant. Among the various specific growth rates tested, the highest GLA concentration of 697 mg/l was obtained in the culture with a specific growth rate of 0.08 /h. Interestingly, the fatty acid profile of the yeast recombinant bearing the Mucor ${\Delta}^6$-desaturase gene was similar to that of blackcurrant oil, with both containing similar proportions of n-3 and n-6 essential fatty acids.

• Journal of Photoscience
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• 제1권1호
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• pp.53-59
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• 1994

Molecular orientation and polarity of solubilization site of dipolar azobenzenes solubilized in micellar solutions are discussed. The polarity of solubilization was estimated by using Taft $\pi$$^*$ scale with linear solvation energy relationship, $\Delta$E=$\Delta$E$_0$ + S($\pi$$^*$ + d$\delta$)+a$\alpha$ + b$\beta$. Hydrogen bonding effects were taken into account for the estimation of micropolarity. The polarity that azobenzenes experienced in the miceliar solutions was close to water which represented that the azobenzenes were mostly solubilized at the interface. For the orientations of azobenzenes were concerned, the nitro group of NPNOH faced the interface and the hydroxy group of NPNO$^-$ located at the interfacial area.

• Molecules and Cells
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• 제25권2호
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• pp.172-177
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• 2008

Eukaryotic translation initiation factor 5B (eIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, and promotes joining of the 60S ribosomal subunit. To see whether the eIF5B proteins of other organisms function in Saccharomyces cerevisiae, we cloned the corresponding genes from Oryza sativa, Arabidopsis thaliana, Aspergillus nidulans and Candida albican and expressed them under the control of the galactose-inducible GAL promoter in the $fun12{\Delta}$ strain of Saccharomyces cerevisiae. Expression of Candida albicans eIF5B complemented the slow-growth phenotype of the $fun12{\Delta}$ strain, but that of Aspergillus nidulance did not, despite the fact that its protein was expressed better than that of Candida albicans. The Arabidopsis thaliana protein was also not functional in Saccharomyces. These results reveal that the eIF5B in Candida albicans has a close functional relationship with that of Sacharomyces cerevisiae, as also shown by a phylogenetic analysis based on the amino acid sequences of the eIF5Bs.

• 한국세라믹학회지
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• 제38권12호
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• pp.1104-1109
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• 2001

110K상의 산화물 고온초전도체를 B $i_{1.84}$P $b_{0.34}$S $r_{1.91}$C $a_{2.03}$C $u_{3.06}$ $O_{10+}$$\delta$/의 출발조성비로 고상반응법(solid-state reaction)에 의해 합성하였다. 이렇게 합성된 Bi계 110K상의 산화물 고온초전도 물질을 다시 분말 상태로 만든 후, Ag 금속분말을 10wt%, 30wt%, 50wt%의 각 비율로 혼합하였다. Ag 금속분말이 혼합된 시편들을 86$0^{\circ}C$~875$^{\circ}C$로 24시간 동안 최종 소결시켰다. 그후, 각 시편들에 대하여 x-선, $T_{c}$, SEM 등을 측정하여 Ag 혼합량에 대한 초전도특성 및 표면의 grain 크기변화 등에 대한 조사를 진행하였다. Ag 혼합량이 증가됨에 따라 Ag peak의 강도는 증가되었고, 시편 내 2223 상의 비율은 감소하고 2212상의 비율은 증가되었다.비율은 증가되었다.

• 대한수학회논문집
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• 제10권1호
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• pp.93-107
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• 1995

In their paper [2,3], Cameron and Storvick introduced some classes $S"+m$ and of functionals on classical Wiener spaces $C_0[a,b]$. For such functionals, they showed that the analytic Feynman integral exists and they gave some formulas for this integral. Moreover they obtained that the functionals of the form $$ (1.1) F(x) = exp {\int^b_a{\theta(s,x(x))dx} $$ are in S" where they assumbed that the potential $\delta : [a,b] \times R \to C$ satisfies (i) for each $s \in [a,b], \theta(s,\cdot)$ is the Fourier-Stieltjes transform of $\sigma_s \in M(R)$, (ii) for each Borel subset E of $[a,b] \times R, \sigma_s (E^{(s)})$ is a Borel measurable function of s on [a,b], and (iii) the total variation $\Vert \sigma_s \Vert$ of $\sigma_s$ is bounded as a function of s.tion of s.

• 한국결정성장학회지
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• 제22권1호
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• pp.29-35
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• 2012

Special attention in the role of convection in vapor crystal growth has been paid since some single crystals under high gravity acceleration of $10g_0$ appear considerably larger than those under normal gravity acceleration ($1g_0$). With increasing the gravity acceleration from $1g_0$ up to $10g_0$, the total molar flux for ${\Delta}T$ = 30 K increases by a factor of 4, while for ${\Delta}T$ = 90, by a factor of 3. The maximum molar fluxes for three different gravity levels of $1g_0$, $4g_0$ and $10g_0$, appear approximately in the neighborhood of y = 0.5 cm, and the molar fluxes show asymmetrical patterns, which indicate the occurrence of either one single or more than one convective cell. As the gravitational level is enhanced form $1g_0$ up to $10g_0$, the intensity of convection is increased significantly through the maximum molar fluxes for ${\Delta}T$ = 30 K and 90 K. At $10g_0$, the maximum total molar flux is nearly invariant for for ${\Delta}T$ = 30 K and 90 K. The total molar flux increases with increasing the gravity acceleration, for $1g_0{\leq}g_y{\leq}10g_0$, and decreases with increasing the partial pressure of component B, a noble gas called as Kr (Krypton), $P_B$. The ${{\mid}U{\mid}}_{max}$ is directly proportional to the gravity acceleration for 20 Torr $P_B{\leq}300$ Torr. As the partial pressure of $P_B$ (Torr) decreases from 300 Torr to 20 Torr, the slopes of the ${{\mid}U{\mid}}_{max}s$ versus the gravity accelerations increase from 0.29 sec to 0.54 sec, i.e. by a factor of 2. The total molar flux of $Hg_2Cl_2$ is first order exponentially decayed with increasing the partial pressure of component B, $P_B$ (Torr) from 20 Torr up to 300 Torr.

• 대한화학회지
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• 제56권4호
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• pp.416-423
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• 2012

Using time domain reflectometry, the complex dielectric spectra between 10 MHz to 20 GHz has been measured in the whole composition range at 10, 20, 30 and $40^{\circ}C$ for the binary mixtures of ethylene glycol and dimethyl sulfoxide. For all the mixtures, only one dielectric loss peak was observed in this frequency range. The relaxation in these mixtures can be described by a single relaxation time using the Debye model. A systematic variation is observed in dielectric constant (${\varepsilon}_0$) and relaxation time (${\tau}$). The excess permittivity (${\varepsilon}^E$), excess inverse relaxation time $(1/{\tau})^E$, Kirkwood correlation factor (g) and thermodynamic parameters viz. enthalpy of activation (${\Delta}H$) and Gibbs free energy of activation (${\Delta}G$) have been determined, to confirm the formation of hydrogen bonded homogeneous and heterogeneous cooperative domains, the dynamics of solute - solute interaction and the hindrance to molecular rotation in the hydrogen bonded glass forming ethylene glycol - dimethyl sulphoxide system.

• Journal of Photoscience
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• 제2권2호
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• pp.89-93
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• 1995

Solvatochromic effects and hydrogen bonding interactions of 4-hydroxy-4'-nitrostilbene(NSOH) were investigated. Electronic transition energies of NSOH have good correlation with Taft's $\pi$$^*$. The hydrogen bonding interactions can be accounted by analysis with equation of $\Delta$E= $\Delta$E$_0$ + s($\pi$$^*$ + d$\delta$) + a$\alpha$ + b$\beta$. From UV-visible absorption maximum energies, $\Delta$E = 81.2 kcal/mole - 6.66($\pi$$^*$ - 0.20$\delta$) - 0.66$\alpha$ - 1.93$\beta$ (corr.=0.970) was obtained, and from fluorescence intensity maximum energies, $\Delta$E= 70.07 kcal/mole - 21.6($\pi$$^*$ - 0.11d) - 1.74$\alpha$ - 2.73$\beta$ (corr.=0.947) was obtained. The micropolarity estimated at the solubilization sites is close to the polarity of water. The apparent Taft's $\pi$$^*$ values of solubilization sites are close to 1. Howcver, the solubilization sites for the fluorescent NSOH is rather nonpolar. Both NSOH and NSO$^-$ can be solubilized in CTAB and CTAC micelles and NSOH is the only species that can be solubilized in SDS and Brij-35 micelles.

• 전자공학회논문지
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• 제54권5호
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• pp.128-132
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• 2017

본 논문에서는 비유전율 38을 갖는 유전체 공진기를 이용하여 S-Band 내 대역폭 20MHz를 갖는 대역통과 필터를 설계 및 제작하였다. 제작에 사용한 유전체 공진기는 $TE_{01{\delta}}$ 모드로 동작하며 중심이 관통된 링형이다. 대역통과 필터는 여섯 개의 유전체 공진기를 이용하여 6-Pole로 구성하였으며 비인접 결합을 통해 타원함수 응답을 구현하였다. 대역통과 필터는 대역폭 내에서 평균 0.97dB의 삽입손실과 25dB 이상의 반사손실을 나타내었다. 비인접 공진기 간의 결합특성으로 체비세프 응답 대역통과 필터에 비해 주파수 선택도를 더욱 향상시킬 수 있었다.

• 대한치과교정학회지
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• 제31권5호
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• pp.535-548
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• 2001

본 연구는 소구치 발치와 전치부 후방 이동을 통한 교정 치료시 이부 연조직의 형태 변화와 주위 경조직과의 상관관계를 알아보기위해 시행되었다. 초진시 구순 돌출감을 보인 Angle분류 I급 또는 II급 1류 부정교합의 환자로서 상하악의 제 1 또는 제 2 소구치를 발치하고 상하악 전치의 후방 이동을 시행한 성인 여성 환자 35명을 대상으로 하였다. 치료전후의 측모두부방사선사진을 계측하였으며, 특히 이부 연조직을 재현성있는 여섯 부분으로 세분화하여 살펴보았고 다음과 같은 결론을 얻었다. 1. 치료전 이부 연조직과 경조직간의 상관 관계를 살펴본 결과 B-B'와 Pm-Pm'는 수직 골격 계측 항목인 $MP{\perp}HP,\;MP{\perp}PP$, ALFH와 유의 한 순상관관계 (P<0.001)를 보이고 a-a', b-b', Me-Me'는 이부 형태 계측 항목인 SL, SW, PL와 유의한 역상관관계(P<0.01)를 보였다. 2. 이부 연조직은 치료전후에 B-B'과 Pm-Pm'에서는 유의한 감소를 보였고(P<0.001), a-a', b-b'에서는 유의한 증가(P<0.01)를 보였다. 3.치료후 이부 연조직 변화는 B-B'의 변화를 제외한 모든 부위에서 경조직의 변화와 상관관계를 보였으며 상관 계수는 0.3-0.4정도였다. 4. 치료후 Pog-Pog'이 증가한 집단과 감소한 집단간에 ${\Delta}UI-VP,\;LI{\perp}의 계측 항목이 유의한 차이를 보였다. 5. 치료후 Me-Me'이 증가한 집단과 감소한 집단간에 ${\Delta}overbite,\;NPog{\perp}HP,\;Me-Me'$의 계측 항목이 유의한 차이를 보였다.