• Molecular & Cellular Toxicology
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• v.5 no.4
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• pp.310-316
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• 2009

Some environmental chemicals have been shown to cause liver-toxicity as the result of bioaccumulation. Particularly, fungicides have been shown to cause varying degrees of hepatictoxicity and to disrupt steroid hormone homeostasis in in vivo models. The principal objective of this study was to evaluate the liver-toxic responses of environmental chemicals-in this case selected fungicides and parasiticides-in order to determine whether or not this agent differentially affected its toxicogenomic activities in hepatic tumor cell lines. To determine the gene expression profiles of 3 fungicides (triadimefon, myclobutanil, vinclozolin) and 1 parasiticide (dibutyl phthalate), we utilized a modified HazChem human array V2. Additionally, in order to observe the differential alterations in its time-dependent activities, we conducted two time (3 hr, 48 hr) exposures to the respective IC20 values of four chemicals. As a result, we analyzed the expression profiles of a total of 1638 genes, and we identified 70 positive significant genes and 144 negative significant genes using four fungicidic and parasiticidic chemicals, using SAM (Significant Analysis of Microarray) methods (q-value<0.5%). These genes were analyzed and identified as being related to apoptosis, stress responses, germ cell development, cofactor metabolism, and lipid metabolism in GO functions and pathways. Additionally, we found 120 genes among those time-dependently differentially expressed genes, using 1-way ANOVA (P-value<0.05). These genes were related to protein metabolism, stress responses, and positive regulation of apoptosis. These data support the conclusion that the four tested chemicals have common toxicogenomic effects and evidence respectively differential expression profiles according to exposure time.

• Molecules and Cells
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• v.41 no.3
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• pp.179-187
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• 2018

Proteomic analysis of extracellular vesicles (EVs) from biological fluid is a powerful approach to discover potential biomarkers for human diseases including cancers, as EV secreted to biological fluids are originated from the affected tissue. In order to investigate significant molecules related to the pathogenesis of bladder cancer, EVs were isolated from patient urine which was analyzed by mass spectrometry based proteomics. Comparison of the EV proteome to the whole urine proteome demonstrated an increased number of protein identification in EV. Comparative MS analyses of urinary EV from control subjects and bladder cancer patients identified a total of 1,222 proteins. Statistical analyses provided 56 proteins significantly increased in bladder cancer urine, including proteins for which expression levels varied by cancer stage (P-value < 0.05). While urine represents a valuable, non-invasive specimen for biomarker discovery in urologic cancers, there is a high degree of intra- and inter-individual variability in urine samples. The enrichment of urinary EV demonstrated its capability and applicability of providing a focused identification of biologically relevant proteins in urological diseases.

• Fire Science and Engineering
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• v.24 no.6
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• pp.54-60
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• 2010

An experimental study has been carried out to determine the flow rate for a feasible N2 generator to extinguish the fire, and this study analogized the correlations to determine the flow rate for $N_2$ generator considered an Oxygen concentration, protected enclosure, discharging pressure and discharging time. We manufactured simple protected enclosure for analyzing fire-extinguishing performance of the $N_2$ generator. As a $N_2$ gas is exhausted on protected enclosure, a various of Oxygen concentration is measured to analyze fire-extinguishing performance experimentally. The correlations determined as an uncertainty analysis for the Oxygen concentration deviations of the theoretical and experimental value. The analogized correlations is Q = (21 $\times$ V)/($O_2+{\zeta}{\cdot}P$)-V. In case of $300m^3$ protected enclosure, 0.8 MPa discharging pressure and $40m^3$/min $N_2$ flow rate, the Oxygen concentration is decreased below 15% within 3 minutes.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.686-693
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• 2000

Biomass yield, nutritive value and nutrient utilization efficiency of different genera of duckweed (DW, Lemnaceae) under the same nutritional and management conditions were studied. Three genera of DW namely: Lemna perpusilla, Spirodela polyrhiza and Woljfia arrhiza, were cultivated in 18 plastic sinks each of $59{\times}54{\times}35cm^3$ size. Each sink contained approximately 80 L of water and was charged once with 6.648 kg of anaerobically fermented cow dung effluent. The seed rate for Spirodela, Lemna and Wolffia were 600, 400 and $600g/m^2$ respectively. Duckweed were harvested at 48 hours intervals. Media total N concentration for Lemna and Wolffia gradually increased with time. Growth of duckweed was measured by subtracting the inoculum from the total biomass production. Growth of Spirodela ceased within 4 days but Lemna and Wolffia continue to grow up to 34 days. Bio-mass yield was significantly (p<0.05) higher in Wolffia (906 kg/ha/d) than Lemna (631 kg/ha/d). Dry matter (DM) yield was very similar in Lemna (14.80 kg/ha/d) and Wolffia (14.57 kg/ha/d). The N content was non-significant higher in Lemna (5.45%) than Wolffia (5.00%) and Spirodela (4.6%). The crude protein (CP, $N{\times}6.25$) yield was non-significantly higher in Lemna (4.83 kg/ha/d) than Wolffia (4.32 kg/ha/d). The acid detergent fibre (ADF) content was the highest in Wolffia (28.59%), followed by Spirodela (19.47%) and Lemna (12.39%). Utilization efficiency of CP was 273 and 314% respectively for Wolffia and Lemna. However, similar efficiency values for Spirodela was only 1.5%. Considering the bio-mass yield, nutritive value and nutrient utilization efficiency, production performance of DW were in the order of Lemna>Wolffia>Spirodela under the present experimental conditions.

• JSTS:Journal of Semiconductor Technology and Science
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• v.13 no.6
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• pp.581-588
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• 2013

This paper presents a study of the process temperature dependence of $Al_2O_3$ film grown by thermal atomic layer deposition (ALD) as a passivation layer in the crystalline Si (c-Si) solar cells. The deposition rate of $Al_2O_3$ film maintained almost the same until $250^{\circ}C$, but decreased from $300^{\circ}C$. $Al_2O_3$ film deposited at $250^{\circ}C$ was found to have the highest negative fixed oxide charge density ($Q_f$) due to its O-rich condition and low hydroxyl group (-OH) density. After post-metallization annealing (PMA), $Al_2O_3$ film deposited at $250^{\circ}C$ had the lowest slow and fast interface trap density. Actually, $Al_2O_3$ film deposited at $250^{\circ}C$ showed the best passivation effects, that is, the highest excess carrier lifetime (${\tau}_{PCD}$) and lowest surface recombination velocity ($S_{eff}$) than other conditions. Therefore, $Al_2O_3$ film deposited at $250^{\circ}C$ exhibited excellent chemical and field-effect passivation properties for p-type c-Si solar cells.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.12 no.4
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• pp.1-10
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• 2013

This study was conducted to investigate that bus information was used as an information of travel speed. To determine the travel speed on the road, bus information and the information collected from the point detector and the interval detection installed were compared. If bus information has the function of traffic information detector, can provide the travel speed information to road users. To this end, the model of recognizing the traffic patterns is necessary. This study used simple moving-average method, simple exponential smoothing method, Double moving average method, Double exponential smoothing method, ARIMA(Autoregressive integrated moving average model) as the existing methods rather than new approach methods. This study suggested the possibility to replace bus information system into other information collection system.

• Korean Journal of Environment and Ecology
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• v.14 no.1
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• pp.46-56
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• 2000

Vegetation structure of $Hwangs\v{o}ng$ Park in $Ky\v{o}ugju$ was investigated to study ecological value and to suggest a desirable management proposal. To do this, twenty plots were set up and surveyed. The $Hwangs\v{o}ng$ Park forest was classified into five plant communities by the DCA technique, which were two types of Pinus densiflora communities, Quercus spp.-P. densiflora, Robinia pseudoacacia and Q. mongolica community. In the diversity analysis, 29 woody species were observed, but species composition was simple. No. of average species is $5.4\pm2.9$ per a plot($100m^2$), and Pinus densiflora is a dominant species in DBH $27\sim47cm$ class. From the results of tree ring and growth analysis, it was found out that pinus densiflora community of $Hwangs\v{o}ng$ Park has a historical and ecological value, but recently the growth rate was decreasing. The management proposal was suggested upon to the above results.

• Proceedings of the KSR Conference
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• 2005.05a
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• pp.299-307
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• 2005

The 180 km/h Korean Tilting Train(TTX) which is now developing as a part of the Korean National R&D project, was elaborately designed. As the tilting trains run curve track with the $30\%$ higher speed than normal trains, the higher centrifugal and dynamic force are expected. Furthermore the complex tilting system increase the probability of failure. Therefore it is very important for tilting train to ensure safety against derailment under the various kind of failed condition in the middle of running as well as normal operating condition. The TTX train have the relatively high roll stiffness to improve the lateral ride comfort and to limit the roll displacement on the curve. But the higher roll stiffness increase the risk of derailment on the twisted track. This paper describes the study to review the safety against derailment caused by the wheel unloading on the severely twisted track. The worst combination of maximum cant change with maximum twist defect was established by numerical simulation. And also it was assumed that the air bag deflated and still the train run its speed limit. Those kind of assumption might be the worst case from the view point of wheel unloading derailment on the twisted track. The dynamic simulation was done by means of VAMPIRE S/W and non-linear transient analysis. We found that derailment quotients Q/P was only slightly influenced by track twist but the wheel unloading was greatly influenced. And we ascertained that the higher roll stiffness the higher wheel unloading. In case of air bag deflated situation, the wheel unloading reached up to $100\%$ which means the wheel lift or jumped. Therefore it was concluded that the design need to be improved to ensure the safety against derailment on the maximum twisted track in case of air bag deflated and tilting train's speed limit.

• Journal of Energy Engineering
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• v.7 no.1
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• pp.65-72
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• 1998

This paper presents a fast calculation method for evaluating of voltage stability margin (MW) using the line flow equation in polar form. Here, Line flow equations $(P_{ij},\;Q_{ij}$ are comprised of state variable, $V_i,\;{\Delta}_i,\;V_j$ and ${Delta}_j$, and line parameter, r and x. using the feature of polar coordinate, these becomes one equation with two variables, $V_j,;V_j$. Moreover, if bus j is slack or generator bus, which is specified voltage magnitude in load flow calculation, it becomes one equation with one variable $V_ i $ that is, may be formulated with the second-order equation for $V^2_i$. Therefore, multiple load flow solutions may be obtained with simple computation. The obtained load flow multiple solutions are used for evaluating of voltage stability through sensitivity analysis or its closeness. Also, the method is proposed to calculate for voltage stability margin considering shunt capacitor, which is important element for evaluating of voltage stability. The proposed method was validated to sample systems.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.6
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• pp.348-355
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• 2017

Aster yomena (AY) have been used as a traditional medicine to treat cough, bronchial asthma, and insect bites in Korea. In this study, we evaluated the inhibition of adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice by AY ethanol extract. Lipid accumulation measurement indicates that AY markedly inhibited adipogenesis in a dose-dependent manner. qRT-PCR results demonstrated that the mRNA expression of adipogenic transcription factors such as peroxisome proliferator-activated receptor-${\gamma}$ ($PPAR-{\gamma}$) in 3T3-L1 cells were significantly down-regulated by AY treatment. And inhibited the expression of FAS, a protein responsible for lipid synthesis, transport and storage. Oral administration of AY (100, 250, and 500 mg/kg, P.O/daily for 4 weeks) was conducted in high-fat diet induced obese mice and C57BL/6 mice. AY was orally administered for 4 weeks to extract liver and epididymal fat, and hematoxylin and eosin staining(H&E staining) was observed. Observation showed that the fat concentration of liver tissue tended to decrease dose-dependently and decreased significantly at 500 mg/kg concentration. The AY-administered group of HFD-induced mice had a lower body weight gain, along with decreased triglycerides and total cholesterol compared with the control mice, however, the HDL-cholesterol/total cholesterol ratio was increased. These results indicate that AY exhibits anti-obesity effects in obese mice by decreasing in serum lipid levels and lipogenesis related gene.