Pseudomonas aeruginosa is a Gram (-) opportunistic human pathogen causing a wide variety of infections on lung, urinary tract, eyes, and burn wound sites and quorum sensing (QS), a cell density-sensing mechanism plays an essential role in Pseudomonas pathogenesis. In order to investigate the importance of QS in the Pseudomonas infections of Korean patients, we isolated 189 clinical strains of P. aeruginosa from the patients in Pusan Paik Hospital, Busan, South Korea. The QS signal production of these clinical isolates was measured by signal diffusion assay on solid media using reporter strains. While most clinical strains (79.4%) produced the QS signals as similar level as a wild type strain, PAO1 did, where LasR, the initial QS signal sensor-regulator was fully activated, a minority of them (4.2%) produced much less QS signals at the level to which LasR failed to respond. Similarly, while 72.5% of the clinical isolates produced QS signals enough to activate QscR, an another QS signal sensor-regulator, some few of them (9%) produced the QS signals at much lower level where QscR was not activated. For further analysis, we selected 74 clinical strains that were obtained from the patients under suspicion of Pseudomonas infection and investigated the total protease activity that is considered important for virulence. Interestingly, significant portion of them showed very low protease activity (44.6%) or no detectable protease activity (12.2%). When the biofilm-forming ability that is considered very important in chronic infection was examined, most isolates showed lower biofilm-forming activity than PAO1. Similarly, significant portion of clinical isolates showed reduced motility (reduced swarming activity in 51.4% and reduced twitching activity in 41.9%), or non-detectable motility (swarming-negative in 28.4% and twitching-negative in 28.4%). Our result showed that the clinical isolates that produced QS signals at the similar level to wild type could have significantly reduced activities in the protease production, biofilm formation, and motility, and some clinical isolates had unique patterns of motility, biofilm formation, and protease production that are not correlated to their QS activity.
D. W. KANG, J. S. CHOI, and G. R. TACK, A Study on Real-Time Sports Activity Classification & Monitoring Using a Tri-axial Accelerometer. Korean Jouranl of Sport Biomechanics, Vol. 18, No. 2, pp. 59-64, 2008. This study was conducted to study the real-time sports activity classification and monitoring using single waist mounted tri-axial accelerometer. This monitoring system detects events of sports activities such as walking, running, cycling, transitions between movements, resting and emergency event of falls. Accelerometer module was developed small and easily attachable on waist using wireless communication system which does not constrain sports activities. The sensor signal was transferred to PC and each movement pattern was classified using the developed algorithm in real-time environment. To evaluate proposed algorithm, experiment was performed with several sports activities such as walking, running, cycling movement for 100sec each and falls, transition movements(sit to stand, lie to stand, stand to sit, lie to sit, stand to lie and sit to lie) for 20 times each with 5 healthy subjects. The results showed that successful detection rate of the system for all activities was 95.4%. In this study, through sports activity monitoring. it was possible to classify accurate sports activities and to notify emergency event such as falls. For further study, the accurate energy consumption algorithm for each sports activity is under development.
This study is to compare irrigation efficiency between sap flow sensor automated system (SF) and conventional irrigation system based on integrated solar radiation automated system (ISR) in tomato rockwool hydroponics. Total irrigated volumes was higher in the ISR system by 5.0L per plant, a lower drainage rate was found in the SF system, compared to the ISR system. There was no difference in shoot and fruit fresh weights, water use efficiency (WUE) and water amount consumed for producing 200g of tomato fruit. The daily average sap flow density (SFD) was closer to the change of solar irradiance (SI) in the plant grown under the SF system, compared to the ISR system. The correlation coefficient (r2) between the fruit diameter and the volumetric water content during the 56 and 82 days after transplant showed the SF treatment was higher than the ISR at night and daytime, and the correlation was higher at night time. The sap flow density and humidity deficit (HD) of SF treatment was related as closely as the solar irradiance. Further studies should demonstrate that SF irrigation system is a convenient method for hydroponic farmers with advantages, such as growth, higher yield, WUE, and accuracy.
The 2-dimensional arrangement method of nodes has been used in most of RF (Radio Frequency) based communication network simulations. However, this method is not useful for the an none-obstacle 3-dimensional space networks in which the propagation delay speed in communication is very slow and, moreover, the values of performance factors such as the communication speed and the error rate change on the depth of node. Such a typical example is an underwater communication network. The 2-dimensional arrangement method is also not useful for the RF based network like some WSNs (Wireless Sensor Networks), IBSs (Intelligent Building Systems), or smart homes, in which the distance between nodes is short or some of nodes can be arranged overlapping with their different heights in similar planar location. In such cases, the 2-dimensional network simulation results are highly inaccurate and unbelievable so that they lead to user's erroneous predictions and judgments. For these reasons, in this paper, we propose a method to place uniformly and randomly communication nodes in 3-dimensional network space, making the wireless link with neighbor node possible. In this method, based on the communication rage of the node, blocks are generated to construct the 3-dimensional network and a node per one block is generated and placed within a block area. In this paper, we also introduce an algorithm based on this method and we show the performance results and evaluations on the average time in a node generation and arrangement, and the arrangement time and scatter-plotted visualization time of all nodes according to the number of them. In addition, comparison with previous studies is conducted. As a result of evaluating the performance of the algorithm, it was found that the processing time of the algorithm was proportional to the number of nodes to be created, and the average generation time of one node was between 0.238 and 0.28 us. ultimately, There is no problem even if a simulation network with a large number of nodes is created, so it can be sufficiently introduced at the time of simulation.
In this study, we applied AEC(Auto Exposure Control), which is used in many chest examinations, to evaluate whether medical devices inserted into the body affect the dose and image quality of chest images. After attaching three HIMD(Human implantable medical devices) to the ion chamber, the Monte Carlo methodology-based program PCXMC(PC Program for X-ray Monte Carlo) 2.0 was applied to measure the effective dose by inputting the DAP(Dose Ares Product) value derived from the Pacemaker and CRT and Chemoport Additionally, to evaluate image quality, we set three regions of interest and one noise region on the chest and measured SNR and CNR. The final study results showed significant differences in DAP and Effective dose. There was a significant difference between Pacemaker and CRT when AEC was applied and not applied. (p<0.05) When applied, the dose increased by 37% for Pacemaekr and 52% for CRT. Chemoport showed a 10% increase in effective dose depending on whether AEC was applied, but there was no significant difference. (p>0.05) In the image quality evaluation, there was no significant difference in image quality between all HIMD insertions and AEC applied or not. (p>0.05) Therefore, when the HIMD was inserted into the chest during a chest x ray and overlapped with the ion chamber sensor, the effective dose increased, and there was no difference in image quality even at a low dose without AEC. Therefore, when performing a chest X-ray examination of a patient with a HIMD inserted, it is considered that performing the examination without applying AEC is a method that can be considered to reduce the patient's radiation exposure.
Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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v.32
no.5
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pp.505-513
/
2014
The RapidEye can acquire the 6.5m spatial resolution satellite imagery with the high temporal resolution on each day, based on its constellation of five satellites. The image products are available in two processing levels of Basic 1B and Ortho 3A. The Basic 1B image have radiometric and sensor corrections and include RPCs (Rational Polynomial Coefficients) data. In Korea, the geometric accuracy of RapidEye imagery can be improved, based on the scaled national digital maps that had been built. In this paper, we present the fully automated procedures to georegister the 1B data using 1:25,000 digital maps. Those layers of map are selected if the layers appear well in the RapidEye image, and then the selected layers are RPCs-projected into the RapidEye 1B space for generating vector images. The automated edge-based matching between the vector image and RapidEye improves the accuracy of RPCs. The experimental results showed the accuracy improvement from 2.8 to 0.8 pixels in RMSE when compared to the maps.
Journal of the Korea Academia-Industrial cooperation Society
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v.20
no.11
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pp.224-231
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2019
Recently, the pollution of groundwater has become serious. In particular, the contamination of groundwater near livestock farms is becoming increasingly severe and it is difficult to drink with drinking water. In this paper, a groundwater purifier apparatus that can be installed in a community well was designed. The designed groundwater purifier apparatus enables a RO membrane filter and UV sterilization to remove pollutants, such as heavy metals, bacteria, and organic compounds. In addition, electrical conductivity, pressure, and flow sensors were added for remote monitoring. Remote monitoring of the system can determine the level of fouling and contamination of RO membrane filters through pressure and flow sensor data, and can record changes in the contamination and condition of groundwater through the electrical conductivity of the feed water. The designed groundwater purifier apparatus was installed at a farmhouse and remote monitoring. The result after 15 days of operating a groundwater purifier apparatus and analyzing the monitoring data revealed an average permeate water flow rate of 2.67L/min and an average water pressure of 7.09kgf/㎠, indicating that the RO Membrane filtered without fouling and clogging. The average electrical conductivity was 796.6 S/㎠ of the feed water and 55.6 S/㎠ of permeate water, which is similar to that of general tap water. Through this, it was confirmed that no pollutant occurred in the surroundings. Therefore, the designed groundwater purifier apparatus can confirm the replacement time of the RO membrane filter in advance through remote monitoring, and check the pollution state of the groundwater.
KOMPSAT-3A images have been used in various kinds of applications, since its launch in 2015. However, there were limits to scientific analysis and application extensions of these data, such as vegetation index estimation, because no tool was developed to obtain the surface reflectance required for analysis of the actual land environment. The surface reflectance is a product of performing an absolute atmospheric correction or calibration. The objective of this study is to quantitatively verify the accuracy of top-of-atmosphere reflectance and surface reflectance of KOMPSAT-3A images produced from the OTB open-source extension program, performing the cross-validation with those provided by a site measurement data of RadCalNet, an international Calibration/Validation (Cal/Val) portal. Besides, surface reflectance was obtained from Landsat-8 OLI images in the same site and applied together to the cross-validation process. According to the experiment, it is proven that the top-of-atmosphere reflectance of KOMPSAT-3A images differs by up to ± 0.02 in the range of 0.00 to 1.00 compared to the mean value of the RadCalNet data corresponding to the same spectral band. Surface reflectance in KOMPSAT-3A images also showed a high degree of consistency with RadCalNet data representing the difference of 0.02 to 0.04. These results are expected to be applicable to generate the value-added products of KOMPSAT-3A images as analysisready data (ARD). The tools applied in thisstudy and the research scheme can be extended as the new implementation of each sensor model to new types of multispectral images of compact advanced satellites (CAS) for land, agriculture, and forestry and the verification method, respectively.
In this paper, in order to verify the performance of a localization algorithm using GPS as an auxiliary sensor, the algorithm was applied to a hovering-type autonomous underwater vehicle (AUV) to perform a field test. The applied algorithm is an algorithm to improve the accumulated positional error of dead reckoning using doppler velocity logger(DVL) and tilt-compensated compass module (TCM) mounted on the AUV. GPS when surfaced helps the algorithm to estimate the position and the heading bias error of TCM for geodetic north, which makes it possible to perform dead reckoning on north-east-down (NED) coordinates. As a result of field test performing heading control, it was judged that the algorithm could improve the positional error, enhance the operational capability of AUV and contribute to the research of underwater navigation depending on a magnetic compass.
Jin, Yihua;Zhu, Jingrong;Sung, Sunyong;Lee, Dong Kun
Korean Journal of Remote Sensing
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v.33
no.2
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pp.149-158
/
2017
Fine temporal and spatial resolution of image data are necessary to monitor the phenology of vegetation. However, there is no single sensor provides fine temporal and spatial resolution. For solve this limitation, researches on spatiotemporal data fusion methods are being conducted. Among them, FSDAF (Flexible spatiotemporal data fusion) can fuse each band in high accuracy.In thisstudy, we applied MODIS NDVI and Landsat NDVI to enhance time resolution of NDVI based on FSDAF algorithm. Then we proposed the possibility of utilization in vegetation phenology monitoring. As a result of FSDAF method, the predicted NDVI from January to December well reflect the seasonal characteristics of broadleaf forest, evergreen forest and farmland. The RMSE values between predicted NDVI and actual NDVI (Landsat NDVI) of August and October were 0.049 and 0.085, and the correlation coefficients were 0.765 and 0.642 respectively. Spatiotemporal data fusion method is a pixel-based fusion technique that can be applied to variousspatial resolution images, and expected to be applied to various vegetation-related studies.
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