• Journal of Korean Society of Forest Science
• /
• v.98 no.4
• /
• pp.363-369
• /
• 2009

This study was carried out to investigate the effect of ${NO_3}^-$ and ${NH_4}^+$ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus koreanum in 5 L-bioreactor culture. The change in the medium components was also measured during culture. The fresh weignt of adventitious root reached to the highest level of 30.8 g FW/L in the presence of both 50 mM ${NO_3}^-$ and 10 mM $NH_4^+$, representing 3.6-fold increase compared to the 60 mM ${NH_4}^+$ alone. However, as the increase of the portion of ${NH_4}^+$, the root growth was decreased. However, the maximum eleutheroside B, E and E1 contents were $57.3{\mu}g/g$ DW, $188.4{\mu}g/g$ DW and $47.3{\mu}g/g$ DW, with 30 mM, 60 mM and 15 mM total nitrogen source, respectively. Fresh weight of adventitious root increased up to 6.8-fold of inoculum size within 9 weeks. The amounts of ${NH_4}^+$, $K^+$, ${NO_3}^-$ and ${PO_4}^-$ were decreased during culture periods. Based on these results, we suggest that various further studies are required to increase the biomass and the useful secondary metabilites.

• Korean Journal of Environmental Biology
• /
• v.40 no.3
• /
• pp.267-274
• /
• 2022

Excess nitrogen (N) flowing from livestock manure to water systems poses a serious threat to the natural environment. Thus, livestock wastewater management has recently drawn attention to this related field. This study first attempted to obtain the optimal conditions for the further volatilization of NH₃ gas generated from pig wastewater by adjusting the amount of injected magnesia (MgO). At 0.8 wt.% of MgO (by pig wastewater weight), the volatility rate of NH₃ increased to 75.5% after a day of aeration compared to untreated samples (pig wastewater itself). This phenomenon was attributed to increases in the pH of pig wastewater as MgO dissolved in it, increasing the volatilization efficiency of NH₃. The initial pH of pig wastewater was 8.4, and the pH was 9.2 when MgO was added up to 0.8 wt.%. Second, the residual ammonia nitrogen (NH₄⁺-N) in pig wastewater was removed by precipitation in the form of struvite (NH₄MgPO₄·6H₂O) by adjusting the pH after adding MgO and H₃PO₄. Struvite produced in the pig wastewater was identified by field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) analysis. White precipitates began to form at pH 6, and the higher the pH, the lower the concentration of NH₄⁺-N in pig wastewater. Of the total 86.1% of NH₄⁺-N removed, 62.4% was achieved at pH 6, which was the highest removal rate. Furthermore, how struvite changes with pH was investigated. Under conditions of pH 11 or higher, the synthesized struvite was completely decomposed. The yield of struvite in the precipitate was determined to be between 68% and 84% through a variety of analyses.

• Journal of Applied Biological Chemistry
• /
• v.42 no.1
• /
• pp.25-28
• /
• 1999

For investigating the effectiveness of Korean natural zeolite for removal of $NH_4{^+}$ in waste waters containing $NH_4{^+}$ and $Cu^{2+}$, the adsorption of $NH_4{^+}$ and $Cu^{2+}$ by three Korean natural zeolite samples, which contained mordenite and/or clinoptilolite, was measured by adding solutions containing $NH_4{^+}$ and $Cu^{2+}$ or both at a concentration range from 1 to 7 mmol(+)/L of each cation. In the solutions, the zeolite samples adsorbed more amounts of $NH_4{^+}$ than $Cu^{2+}$. By seven successive equilibrations, Korean natural zeolites adsorbed $NH_4{^+}$ corresponding to 23~33% of those cation exchange capacity from the solution containing both $NH_4{^+}$ and $Cu^{2+}$ at 3 mmol(+)/L of each cation. Whereas, the corresponding adsorption of $Cu^{2+}$ was 17-27% of the CEC. Korean natural zeolite exhibited selectivity for $NH_4{^+}$ but not for $Cu^{2+}$. Nevertheless, it using as a soil amendment after removing of $NH_4{^+}$ in waste waters should be carefully controlled on the application rate.

• Horticultural Science & Technology
• /
• v.34 no.5
• /
• pp.727-735
• /
• 2016

This research was conducted to investigate the influence of various pre-planting $NO_3:NH_4$ ratios in the coir dust+peatmoss+perlite (3.5:3.5:3, v/v/v) medium on the growth of tomato plug seedlings (cv. Dotaerang Dia), changes in chemical properties of root media, and tissue nutrient contents. With the fixation of N concentration to $300mg{\cdot}L^{-1}$, the $NO_3:NH_4$ ratios were adjusted to 100:0, 73:27, 50:50, 27:73, 0:100. Then, root media containing various $NO_3:NH_4$ ratios and equal concentration of other essential nutrients were filled into 50-cell plug trays and the seeds of 'Dotaerang Die' tomato were sown. The investigation of seedling growth and tissue analysis for mineral nutrient contents based on the dry weight of above-ground tissue were conducted 6 weeks after sowing. As seedlings grew, the EC decreased quickly and pH increased gradually in the all treatment media. The plant height, fresh weight and dry weight of seedlings in the treatment of 50:50 ($NO_3:NH_4$) were 29.0 cm, 13.7 g, and 1.21 g, respectively, which were the highest among treatments tested. However, the seedlings in the treatment of 0:100 ($NO_3:NH_4$) had 26.5 cm, 11.2 g, and 0.92 g in plant height, fresh weight and dry weight, respectively. These were the poorest among the treatments tested. The tissue contents of N were 2.77 to 3.22% in all the treatments. The treatment of 27:73 ($NO_3:NH_4$) had the highest contents of Fe, Mn and Zn and that of 0:100 ($NO_3:NH_4$) had the lowest contents of Mg, Na, Cu, Mn and Zn among the treatments tested. The results indicate that $NH_4$ ratio should be lower than 50% in the coir dust+peatmoss+perlite (3.5:3.5:3, v/v/v) medium for seedling growth of tomato and the optimum ratio will be used to draw up guide lines for plug seedling production.

• Journal of Industrial Technology
• /
• v.19
• /
• pp.331-341
• /
• 1999

We analyzed theoretically the removal efficiency and the particle growth inside the pulse corona discharge reactor to remove $NO_x$ and investigated the effects of process variables such as the NO and $NH_3$ input concentrations. Most of NO is converted into $NO_2$ and $HNO_3$ and the $HNO_3$ reacts with $NH_3$ to form the $NH_4NO_3$ particles. About 6.4% of NO is converted into $HNO_2$ which form the $NH_4NO_2$ particles by reaction with $NH_3$. Some of $NO_2$ follows the reaction pathway to form $NO_3$ and $N_2O_5$. The amount of particles formed inside the reactor is basically determined by the input $NH_3$ concentration. The ratio of NO to $NH_3$ affects the reactor length for particle formation significantly. The higher the input concentrations of NO and $NH_3$ are, the faster the particles grow.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.46 no.4
• /
• pp.303-308
• /
• 2001

To investigate the partitioning of newly absorbed N derived from NO$_3$- and NH$_4$$^{+}$, 6 mM $K^{15}$ NO$_3$ or 3 mM ($^{15}$ NH$_4$)$_2$ was fed continuously in Italian ryegrass (Lolium multiflrum L.) for 7 days. Nitrogen metabolites (nitrate, amino acid, soluble- and insoluble protein) were analyzed at the end of $^{15}$ N feeding. Dry weight in shoot, stubble and root was not significantly different between NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ feeding. Total nitrogen content in all three organs was significantly higher in NH$_4$$^{+}$ than NO$_3$$^{[-10]}$ feeding. Sum on N content in reduced N fractions (amino acids + proteins) in shoot, stubble and roots in NH$_4$$^{+}$ feeding increased by 13.3, 12.5 and 35.4 %, respectively, compared to NO$_3$$^{[-10]}$ feeding. The Relative Specific Activity (RSA, percentage of newly absorbed $^{15}$ N relative to total N in a sample) values of amino acids and insoluble proteins were significantly higher in NH$_4$$^{+}$ feeding. Total amount of newly absorbed $^{15}$ N in NO$_3$$^{[-10]}$ and NO$_3$$^{[-10]}$ feeding was 52.3 and 69.5 mg/plant on dry matter basis, respectively. In both NH$_4$$^{+}$ and NO$_3$$^{[-10]}$ grown plants, most of the N was allocated to the shoot, 67.5% in NH$_4$$^{+}$ feeding and 58.8% NO$_3$$^{[-10]}$ feeding, respectively. The $^{15}$ N amount incorporated in the reduced N compounds (amino acids and proteins) in NH$_4$$^{+}$ grown plants significantly increased by 74.8% compared to NO$_3$$^{[-10]}$ grown plants. The increase of the $^{15}$ N amount assimilated to amino acids in NH$_4$$^{+}$ grown plants was remarkably higher in roots as more than 7.25 times compared to NO$_3$$^{[-10]}$ feeding. These results indicated that Italian ryegrass was much efficiently utilized NH$_4$$^{+}$-N for the synthesis of reduced N compounds.reduced N compounds.

• Journal of Korean Vacuum Science & Technology
• /
• v.6 no.1
• /
• pp.30-35
• /
• 2002

We show that a combined technique of Cs$^{+}$ reactive ion scattering (Cs$^{+}$ RIS) and low-energy secondary ion mass spectrometry (LESIMS) provides a powerful means for probing molecular films and their surface reactions. Simple molecules, including HCI, NH$_3$, D$_2$O, and their mixtures, were deposited into a thin film of several monolayer thickness on Ru(001) at low temperature in vacuum, and the surface was characterized by Cs$^{+}$ RIS and LESIMS. On pure films, D$_2$O, HCI, and NH$_3$ existed in the corresponding molecular states. When HCI and NH$_3$ were co-deposited, ammonium ion(NH$_4$$^{+}$) was readily formed by proton transfer from HCI to NH$_3$. In the presence of water molecules, HCI ionized first to hydronium ion(H$_3$O$^{+}$), which subsequently transferred proton to NH$_3$ to form NH$_4$$^{+}$. The proton transfer, however, did not occur to a completion on ice, in contrast to the complete reaction in aqueous solutions.s solutions.

• YAKHAK HOEJI
• /
• v.7 no.4
• /
• pp.98-99
• /
• 1963

Compound III and IV were formed from I and II with $NH_{2}(CH_{2})_{3}NH_{2}$ respectively. Reduction of I with $LiAlH_{4}$ bollowed by $PoCl_{3}$ treatment affored VIII. Compound VI which was asumed by IR-data were obtained by treatment of $NH_{2}NH_{2}$ upon I or V.

• Asian Journal of Turfgrass Science
• /
• v.9 no.3
• /
• pp.207-212
• /
• 1995

This experiment was conducted to investigate the effect of the nitrogen fertilization levels on the content of $NH_4^+$-N in soil of 'Suffolk' Kentucky bluegrass and zoysiagrass. The results obtained are summarized as follows : 1.According to the nitrogen fertilization levels, the content of $NH_4^+$-N in soil of Kentucky blue-grass and zoysiagrass was not significantly different. The content of $NH_4^+$-N in soil of Kentucky bluegrass and zoysiagrass was highest in June and December and lowest in March and September. 2.The content of $NH_3^-$-N in soil was increased by increasing the nitrogen fertilization levels in both Kentucky bluegrass and zoysiagrass. However, the deeper the depth of soil the less the content of $NO_3^-$-N in soil. In 40~60cm soil depth, the content of $NO_3^-$-N in soil was lower than 10ppm in average. Even in June, which was the highest month of the content of $NO_3^-$-N in soil, the content of $NO_3^-$-N in soil was not overpassed the degree of 20ppm.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.32 no.3
• /
• pp.310-316
• /
• 1987

A field experiment was conducted to find out the effects of liming(soil pH) and sources of N on growing characters and yield of burley tobacco. Treatments consisted of liming(nonliming, liming to soil pH5.5 and 6.5) as the main plot and N sources(compound fertilizer of containing 3.9% NH$_4$-N and 6.1% NH$_2$-N, NaNO$_3$,(NH$_2$)$_2$CO and (NH$_4$)$_2$SO$_4$as the sub-plot. The growth of vegetative growing stage of limed plots were delayed(to compare the nonlimed plot) by influence of alkali. When the source on N was NaNO$_3$ the growth of vegetative growing stage was unfavorable and the yellowing of lower leaves of maturing stage was rapid. The yield and value of cured leaf was increased by increasing the rate of Ca(OH)$_2$, but there was no significant differences among the source of N. The yield response to liming was greater when the source of N was (NH$_4$)$_2$SO$_4$ than that of any other plots.