• The Korea Journal of Herbology
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• v.29 no.3
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• pp.79-85
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• 2014

Objectives : In this study, we attempted to evaluate the effects of Careswell on human mast cell-mediated allergy inflammation in vitro and pruritogen-induced scratching behavior in vivo. Method : The Careswell was extract by distilled water. The anti-itching effects of Careswell were investigated on the compound 48/80 ($50{\mu}g/kg$) or histamine ($100{\mu}g/kg$) induced scratching behavior male ICR mice for 30 min by an observer blind. Terfenadine (10 mg/kg) was used as a positive control drug. The cell toxicity of Careswell was determined by 3-(4,5-dimethylthiazole-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The regulatory effect of Careswell on interleukin (IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ levels was determined by enzyme-linked immunosorbent assay (ELISA) in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) stimulated human mast cells (HMC-1). Also, we evaluated the effect of Careswell on PMACI induced the activation of Nuclear factor-kappa B (NF-${\kappa}B$) into nucleus by Western blot analysis. Result : The results revealed that the oral administration of Careswell (200 mg/kg, p.o.) attenuated the compound 48/80 or histamine-induced scratching behavior in mice. We showed that Careswell significantly reduced the PMACI-induced the production of IL-6 (0.5-1 mg/ml) and TNF-${\alpha}$ (0.1-1 mg/ml). Additionally, Careswell significantly inhibited the activation of NF-${\kappa}B$ in PMACI-stimulated HMC-1. Conclusion : Collectively, the findings of this study provide us with a novel insight into the pharmacological actions of Careswell as a potential molecule for use in the treatment of allergic inflammation diseases.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.07b
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• pp.772-775
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• 2004

본 논문에서는 $B_2O_3$ 첨가가 Ba$(Mg_{1/3}Ta_{2/3})O_3$ (BMT)의 유전특성 및 미세구조의 변화에 미치는 영향에 대해 연구하였다 $B_2O_3$가 소량 첨가되었을 때는 결정립의 성장을 야기하여 치밀한 미세구조를 보였지만, 다량이 첨가된 경우 비정상 결정립 성장을 야기하여 치밀화가 떨어지는 미세구조를 보임과 동시에 $Ba_3Ta_5O_{15}$의 2차상을 형성했다. 이는 소량의 $B_2O_3$ 첨가가 유전특성의 향상을 가져왔지만, 다량의 첨가는 오히려 특성의 악화를 가져온 결과의 원인이라 생각된다. 0.5mol%의 $B_2O_3$를 첨가하여 $1500^{\circ}C$에서 6시간 소결한 경우 ${{\varepsilon}_r}=24$, $Q{\times}f=210,000GHz$의 유전 특성 값과 $4.74ppm/^{\circ}C$의 $T_{cf}$ 값을 얻었다.

• Korean Journal of Veterinary Research
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• v.46 no.3
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• pp.207-214
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• 2006

Mycoplasma gallisepticum (MG) continues to persist in many commercial layer farms in Korea,resulting in losses in egg production. Bacterins and live attenuated vaccines have been used for the prevention of losses caused by MG. One of these attenuated vaccines, MG 6/85 vaccine has been reported to be safe and efficacious in layers. However, MG 6/85 vaccine has not been evaluated for its safety and its efficacy in any commercial layer in Korea. Six-week-old specific pathogen-free (SPF) chickens were vaccinated with MG 6/85 vaccine by aerosol and were challenged with virulent MG R strain at 4 weeks after vaccination. The vaccinated group was able to resist challenge into the air sacs because the vaccinated group showed much less air sac lesion compared with the unvaccinated group. Each of two commercial layer farms was divided into vaccinated and unvaccinated groups. For each vaccinated gorup, MG 6/85 vaccine were sprayed at 17 week old on farm A and at 15 weeks old on farm B. Hen-day egg production, Hen-housed eggs, egg weight, mortality were evaluated until 50 week after vaccination.Compared with the unvaccinated group in each farm, the vaccinated group showed higher average egg production and egg weight, and higher hen-housed number. Results of this study are in agreement with other previous reports which demonstrated that MG 6/85 vaccine favorable effect on performance in commercial layers.

• Journal of Nutrition and Health
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• v.14 no.2
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• pp.59-70
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• 1981

The objective of this study is to induce the primary characteristics of phenylketonuria in infant rats during the first 2 weeks after birth. The critical biochemical parameter in the development of phenylketonuria is the elevation of plasma phenylalanine while tyrosine is maintained at a relatively low level. A PKU-like condition was induced in infant rats during the first 2 weeks after birth using a modification of our previously published procedure for the development of a temporary (1 to 3 days) PKU-like condition. Phenylalanine was administered by stomach intubation every 6 hours (starting at 6:00 a.m.) at a dose level of 400mg per kg body weight (after birth-day 2 thru 5) and 500mg per kg body weight (day 6 thru 14). Amethopterin was given at 0.00625 or 0.0125mg per kg body weight (day 3 thru 14) and p-chlorophenylalanine at 50 mg per kg body weight (day 5 thru 14) at 9:00 a.m. and 9:00 p.m. At the times measured (6,10 and 14 days) plasma phenylalanine/tyrosine (P/T) ratios were elevated from a normal value of the or less to values ranging from 6 to 15. During the second week after birth a staggering gait, abnormal stance and decreased social behavior were also observed. None of these PKU-like characteristics were apparent in the three control groups receiving (a) no phenylalanine or inhibitors, (b) phenylalanine alone, or (c) inhibitors alone. The establishment of these primary biochemical characteristics of phenylketonuria by stomach intubation of phenylalanine and a combination of low dose levels of enzyme inhibitors to infant rats provides an experimental system which should he valuable for extensive biochemical, histological and behavioral studies in phenylketonuria.

• KSBB Journal
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• v.6 no.3
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• pp.289-297
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• 1991

The insecticidal protein gene in the pKL-20-1 clone derived from Bacillus thuringiensis serovar. kurstaki plasmid was subcloned in the plant shuttle vector, pGA643. The 7.3 kb fragment was cloned in the BglII and Hpal sites of pGA643 vector and expressed in E. coli S17-1, which produced insecticidal proteins killing Bombyx mori larvae. The clone was named pHL-20. The protoplast formation, calli induction and plantlet regeneration of Nicotiana tabacum was carried out. A tremendous number of mesophyll protoplasts of N. tabacum were formed, up to 7$\times$105 protoplast per ml, for 20 hours in darkness in the enzyme solution of 0.5% cellulase and 0.1% macerosin, pH 5.8. The viabilities of the protoplasts were maintained above 80% for 6 days in the media containing 2mg/1 of NAA and 1mg/1 of kinetin. Calli were induced from the protoplasts and leaves of the N. tabacum on MS medium containing 0.5mg/1 BAP. Under the culture conditions the protoplasts underwent repeated cell division into calli. Plantlets were regenerated from callus cultures derived from protoplast and leaves. Shoots were induced in a medium containing 1mg/1 of BAP.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.666-672
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• 2002

Changes in chemical characteristic, microflora, and sensory evaluation of fish sauce extracted at an interval of one week from fermented solution were investigated. pH was reduced from 6.0 to 4.5, and trimethylamine oxide from 132.5 to 87.2 mg/100g during fermenting periods. Trimethylamine increased from 5.6 to 50.2 mg/100g, and volatile basic nitrogen from 48.3 to 232.5 mg/100g. Bacterial flora isolated from the fish sauce were 70% Lactobacillus sp. and 13% Bacillus sp. Among the free amino acids, alanine, glutamic acid, valine, and methionine contents constitute 40% of the total free amino acids. Major non-volatile organic acid of the fish sauce was lactic acid (76%). Sensory evaluation results of the fish sauce were higher than the traditional soybean sauce after 28 days of fermentation.

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.696-706
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• 2020

Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 mg/g (200 g of pupa, 1 g of KH₂PO₄, 0.5 g of K₂HPO₄, 20 g of glucose, 1 g of MgSO₄, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 mg/g (15 g of skim milk powder, 1 g of KH₂PO₄, 0.5 g of K₂HPO₄, 20 g of glucose, 1 g of MgSO₄, 0.05 g of vitamin B1, and 1 mg of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

• Journal of the Korean Society for Heat Treatment
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• v.21 no.6
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• pp.293-299
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• 2008

In order to provide with fundamental data of the wrought Mg alloy for press forging, the effect of annealing temperature on the microstructure, texture development and tensile properties is studied in a commercial AZ31B Mg alloy sheet. Basal texture i.e. $(0001){\pm}5^{\circ}$[21$\bar{3}$0] is developed in a commercial AZ31B Mg sheet, and the texture is not changed considerably by annealing over $400^{\circ}C{\times}30min$, while (10$\bar{3}$0) component with high intensity can be observed due to abnormal grain growth. When the sheet is tensile-deformed with RD, $45^{\circ}$ and TD directions at room temperature, fracture strains are given by 25.8, 21.4 and 11.9% in the order of RD, $45^{\circ}$ and TD directions, respectively. With increasing annealing temperature up to $450^{\circ}C{\times}30min$, little change in mean grain size can be revealed by annealing below $300^{\circ}C{\times}30min$ but an abnormal grain growth, where some grains become significantly coarser than the rest, occurs by annealing above $400^{\circ}C{\times}30min$. The maximum tensile strain of around 25% is obtained by annealing below $300^{\circ}C{\times}30min$, but it is abruptly decreased to 16% by annealing above $400^{\circ}C{\times}30min$ owing to intergranular fracture of abnormal grown grains.

• Journal of Microbiology and Biotechnology
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• v.16 no.4
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• pp.519-523
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• 2006

To improve epothilones production and the ratio of epothilone B/A, carbon sources were investigated in flask culture of Sorangium cellulosum. Depending on the initial concentration, starch significantly enhanced cell growth, but the maximum epothilones productivity and the maximum epothilones production (0.3 mg/l day and 2.6 mg/l at 15 g/l starch, respectively) were relatively low compared with cell growth. On the other hand, addition of glycerol did not stimulate cell growth, but epothilone production was increased from 2.81 mg/l to 7.59 mg/l. Addition of glycerol to culture medium resulted in more significant enhancement of the production of epothilone A, whereas epothilone B levels were relatively constant. Furthermore, when sodium propionate was added as a precursor of methylmalonyl-CoA, it resulted in increase of both total epothilones production and epothilone B/A resolution. Maximum epothilone A and B concentrations reached 10.9 mg/l and 8.58 mg/l, respectively, at 5 mM sodium propionate.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.265-270
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• 2004

Plant regeneration from seed-derived callus of Kentucky bluegrass(Poa pratensis L. cv. Kenblue) was investigated. Callus induced on the medium supplemented with 2 mg/L 2,4-D and 0.2 mg/L BAP showed highest frequency of plant regeneration on the regeneration medium supplemented with 1 mg/L NAA and 5 mg/L kinetin. Callus induced in the dark condition showed higher regenerability than that induced in the dim light. MS medium was better than N6 and B5 medium in enhancing plant regeneration. Maltose was superior to sucrose in plant regeneration as carbon source in the medium.