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Purification and Structural Characterization of Glycolipid Biosurfactants from Pseudomonas aeruginoas YPJ-80

  • Park, Oh-Jin;Lee, Young-Eun;Cho, Joong-Hoon;Shin, Hyun-Jae;Yoon, Byung-Dae;Yang, Ji-Won
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.2
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    • pp.61-66
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    • 1998
  • Glycolipids produced by Pseudomonas aeruginosa YPJ-80 were characterized by chromatographic and spectorscopic techniques as a mixture of two rhamnolipids. For recovery of glycolipids from the culture broth, various isolation methods including ultrafiltration, adsorption and solvent extraction were compared. Ultrafiltration showed the best results in terms of glycolipids recovery. Further purification for spectroscopic analysis was carried out by adsorption chromatography and preparative thin layer chromatography. From the spectroscopic analysis, such as IR spectroscopy. FAB-MS, 1H-NMR and 13C-NMR and hydrolysis analysis, the glycolipids were identified as L-${\alpha}$-rhamnopyranosyl-${\beta}$-hydroxydecanoly-${\beta}$-hydroxydecanoate and 2-O-${\alpha}$-L-rhamnopyranosyl-${\alpha}$-L-rhamnopyranosyl-${\beta}$-hydroxydecanoyl-${\beta}$-hydroxydecanoate. Monorhamnolipid and dirhamnolipid lowered the surface tension of water to 28.1 mN/M and 29.3 mN/m, respectively.

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Physicochemical properties of Ginkgetin and Isoginkgetin extracted from Ginko leaves (은행잎 추출물 유래 Ginkgetin 및 Isoginkgetin의 융복합 특성 연구)

  • Min, Kyung-Nam
    • Journal of the Korea Convergence Society
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    • v.9 no.6
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    • pp.89-98
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    • 2018
  • To confirm the feasibility as raw materials for cosmetic formulation, ethanol, n-hexane, ethylacetate and normal butyl alcohol were extracted from 1kg of Ginko leaves. Ultimately, investigated chemical structure and physicochemical properties of ginkgetin and isoginkgetin. The purity of those two components have been evaluated higher than 99%. They are isomers with molecular weight as 566 and a molecular formular, $C_{32}H_{22}O_{10}$. Flavonoid's frame and NMR spectrum exhibited the existence of aromatic proton and the proper ratio of the carbon, which were expected structures exactly corresponded to the reported references.

Characteristics of Schottky Diode and Schottky Barrier Metal-Oxide-Semiconductor Field-Effect Transistors

  • Jang, Moon-Gyu;Kim, Yark-Yeon;Jun, Myung-Sim;Lee, Seong-Jae
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.5 no.2
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    • pp.69-76
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    • 2005
  • Interface-trap density, lifetime and Schottky barrier height of erbium-silicided Schottky diode are evaluated using equivalent circuit method. The extracted interface trap density, lifetime and Schottky barrier height for hole are determined as $1.5{\times}10^{13} traps/cm^2$, 3.75 ms and 0.76 eV, respectively. The interface traps are efficiently cured by $N_2$ annealing. Based on the diode characteristics, various sizes of erbium- silicided/platinum-silicided n/p-type Schottky barrier metal-oxide-semiconductor field effect transistors (SB-MOSFETs) are manufactured from 20 m to 35nm. The manufactured SB-MOSFETs show excellent drain induced barrier lowering (DIBL) characteristics due to the existence of Schottky barrier between source and channel. DIBL and subthreshold swing characteristics are compatible with the ultimate scaling limit of double gate MOSFETs which shows the possible application of SB-MOSFETs in nanoscale regime.

Isolation and Identification of Phenol Compounds from Acer tegmentosum and their Anti-inflammatory Activity (산겨릅나무로부터 페놀화합물의 분리 및 항염증 활성의 측정)

  • Song, Na-Young;Lee, Kwang Jin;Ma, Jin Yeul
    • Korean Journal of Pharmacognosy
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    • v.45 no.2
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    • pp.93-100
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    • 2014
  • The Acer tegmentosum (3 kg) were extracted with boiled water and the freeze dried extract powder was partitioned with $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc and n-BuOH fraction, six phenolic compounds were isolated through the silica gel, octadecyl silica gel and sephadex LH-20 column chromatography. On the basis of spectroscopic methods, such as $^1H$-NMR and $^{13}C$-NMR, and LC/MS, the chemical structures of the compounds as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), salidroside (5) and 6'-O-galloylsalidroside (6). In this study, compounds 1 and 2 have been first isolated from the A. tegmentosum. To provide insight into the effects of six compounds isolated from A. tegmentosum on inflammation, we investigated its effect on nitric oxide (NO) production in RAW 264.7 cells using lipopolysaccharide (LPS) stimulation. Compounds 1 and 6 slightly repressed NO production. Also, compounds 3 and 4 inhibited NO secretion with statistical significance. However, compounds 2 and 5 did not show any inhibitory effect on NO production.

Changes in the Specific Gravity of Pacific Cod Gadus macrocephalus, During the Early Life Stages (대구(Gadus macrocephalus)의 초기 발생시기의 비중변화)

  • Lee, Hwa Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.3
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    • pp.332-337
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    • 2018
  • The Pacific cod Gadus macrocepahlus, lays demersal eggs and the hatching larvae rise toward the surface layer of the ocean to feed. The change in the specific gravity of eggs and larvae was investigated to examine their vertical distribution and movement in the water column. The specific gravities of fertilized eggs and various size classes of larvae were measured using a density gradient apparatus. In total, the instantaneous specific gravity of 146 eggs and 225 larvae were measured. To prevent any disturbance in the gradient water column due to larval movement, 0.004% MS222 was used for anesthesia. Due to their high specific gravity, eggs spawned were deposited over the sea-bed of the spawning ground. The specific gravity of hatching larvae decreased abruptly. However, Pacific cod larvae still had a comparatively high specific gravity at hatching ($1.03655{\pm}0.00146g/cm3$, n=4, mean SL=3.62 mm) and their specific gravities tended to decrease as they grew. The specific gravity stabilized 6 days after hatching ($1.02590{\pm}0.00212g/cm3$, n=15, mean SL=4.67 mm) and the cod larvae were eventually able to float in the water column.

Micro flow sensor using polycrystalline silicon carbide (다결정 실리콘 카바이드를 이용한 마이크로 유량센서)

  • Lee, Ji-Gong;Lei, Man I;Lee, Sung-Pil;Rajgopal, Srihari;Mehregany, Mehran
    • Journal of Sensor Science and Technology
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    • v.18 no.2
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    • pp.147-153
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    • 2009
  • A thermal flow sensor has been fabricated and characterized, consisting of a center resistive heater surrounded by two upstream and one downstream temperature sensing resistors. The heater and temperature sensing resistors are fabricated from nitrogen-doped(n-type) polycrystalline silicon carbide(poly-SiC) deposited by LPCVD(low pressure chemical vapor deposition) on LPCVD silicon nitride films on a Si substrate. Cavities were etched into the Si substrate from the front side to create suspended silicon nitride membranes carrying the poly-SiC elements. One upstream sensor is located $50{\mu}m$ from the heater and has a sensitivity of $0.73{\Omega}$/sccm with ${\sim}15\;ms$ rise time in a dynamic range of 1000 sccm. N-type poly-SiC has a linear negative temperature coefficient and a TCR(temperature coefficient of resistance) of $-1.24{\times}10^{-3}/^{\circ}C$ from room temperature to $100^{\circ}C$.

The Antimicrobial Activity of Essential Oil from Dracocephalum foetidum against Pathogenic Microorganisms

  • Lee, Saet-Byoul;Cha, Kwang-Hyun;Kim, Su-Nam;Altantsetseg, Shataryn;Shatar, Sanduin;Sarangerel, Oidovsambuu;Nho, Chu-Won
    • Journal of Microbiology
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    • v.45 no.1
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    • pp.53-57
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    • 2007
  • A number of essential oils from Mongolian aromatic plants are claimed to have antimicrobial activities. The essential oil of Dracocephalum foetidum, a popular essential oil used in Mongolian traditional medicine, was examined for its antimicrobial activity. Eight human pathogenic microorganisms including B. subtilis, S. aureus, M. lutens, E. hirae, S. mutans, E. coli, C. albicans, and S. cerevisiae were examined. The essential oil of Dracocephalum foetidum exhibited strong antimicrobial activity against most of the pathogenic bacteria and yeast strains that were tested; by both the agar diffusion method and the minimum inhibitory concentration (MIC) assay ($MIC\;range\;was\;26-2592{\mu}g/ml$). Interestingly, Dracocephalum foetidum even showed antimicrobial activity against methicilin-resistant Staphylococcus aureus (MRSA) strains. We also analyzed the chemical composition of the oil by GC-MS and identified several major components, including n-Mentha-1,8-dien-10-al, limonene, geranial, and neral.

Extraction and Bioassay of Allelochemicals in Jerusalem Artichoke

  • Sungwook Chae;Lee, Ho-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.4
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    • pp.309-316
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    • 2001
  • Helianthus tuberosus has been known to inhibit the growth of weeds and other plants sharing its habitat. This study was conducted to identify the allelochemicals of Helianthus tuberosus which were extracted with water and solvents. Aqueous extracts of leaf, stem, root, tuber and tuber peel of Helianthus tuberosus except tuber did not show significant differences in phytotoxicity to alfalfa seedlings. It was considered that Helianthus tuberosus contained fewer or less potential water-soluble substances that were toxic to alfalfa. Methanol extract of leaves of Helianthus tuberosus was sequentially partitioned in increasing polarity with n-hexane, ethylacetate and n-butanol. Each extract had a yield of 148, 12, 15.7 and 9.5g, respectively. Inhibitory effects on germination of alfalfa seeds treated with four fractions were not significantly different. But the significant reductions on hypocotyl length were observed for all the solvent extracts. Among the four fractions, the ethylacetate fraction showed the most significant inhibition effect on bioassay with alfalfa. Further separation of the active ethylacetate fraction by open column chromatography led to the 25 subfractions. In bioassay of each sub-fraction with alfalfa seeds, sub-fraction No. 13 showed the most inhibitory effect on seedling growth. $^1$H NMR and gas chromatography-mass spectrometry analysis revealed that sub-fraction No. 13 was the mixture of straight-chain saturated fatty acids.

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High-Speed Generation Technique of Digital holographic Contents based on GPGPU (GPGPU기반의 디지털 홀로그램 콘텐츠의 고속 생성 기법)

  • Lee, Yoon Hyuk;Kim, Dong Wook;Seo, Young Ho
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.9 no.1
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    • pp.151-163
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    • 2013
  • Recently the attention on digital hologram that is regarded as to be the final goal of the 3-dimensional video technology has been increased. Digital hologram is calculated by modeling the interference phenomenon between an object wave and a reference wave. The modeling for digital holograms is called by computer generated hologram (CGH) Generally, CGH requires a very large amount of calculation. So if holograms are generated in real time, high-speed method should be needed. In this paper, we analyzed CGH equation, optimized it for mapping general purpose graphic processing unit (GPGPU), and proposed a optimized CGH calculation technique for GPGPU by resource allocation and various experiments which include block size changing, memory selection, and hologram tiling. The implemented results showed that a digital hologram that has $1,024{\times}1,024$ resolution can be generated during approximately 24ms, using 1K point clouds. In the experiment, we used two GTX 580 GPGPU of nVidia Inc.

Sensitive Determination of Natural and Synthetic Steroidal Hormones of a Free and Conjugated Form in Surface Water by Gas Chromatography-Mass Spectrometry

  • Shin, Ho-Sang;Oh, Jin-Aa;Shin, Sun-Kyoung
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.809-814
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    • 2011
  • A method based on the TMS derivatives and acidic hydrolysis was developed for the simultaneous determination of free and conjugated steroidal hormones in surface water. A silylation of five natural and two synthetic steroidal hormones was achieved with N-methyl-N-(trimethylsilyl) trifluoroacetamide/$NH_4I$ (1000:3) under catalysis of dithioerythritol for 60 min at $80^{\circ}C$. TMS derivatives of the steroid hormones containing multifunctional groups offer a single derivative product under this condition. The accuracy of the analytes was in the range of 87 to 110% at a concentration of 20 and 50 ng/L with relative standard deviations of less than 10%. The method detection limit was in the range of 0.01 to 0.02 ng/L for surface water. Natural steroidal hormones were detected in a concentration range of 0 to 1.03 ng/L in free form and 0 to 14.6 ng/L in conjugated form, respectively. We found that most of the natural hormonal steroids exist in conjugate forms (43 to 100%) in river water.