• Title/Summary/Keyword: $MS^n$

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Development of New Molecular Markers for the Identification of Male Sterile Cytoplasm in Peppers (Capsicum annuum L.)

  • Min, Woong-Ki;Kim, Byung-Dong;Kim, Sung-Gil;Lee, Sang-Hyeob
    • Horticultural Science & Technology
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    • v.29 no.1
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    • pp.53-60
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    • 2011
  • Cytoplasmic male sterility (CMS) induced by mutant mitochondria genome, has been used for commercial seed production of $F_1$ hybrid cultivars in diverse crops. In pepper (Capsicum annuum L.), two sterile cytoplasm specific gene organization, atp6-2 and coxII were identified. An open reading frame, orf456 nearby coxII gene has been speculated to induce male sterility (MS) by mutagenic analysis. Moreover, molecular markers for atp6-2 and coxII of mitochondrial genotype (mitotype) were developed. However, the Cytoplasmic MS specific markers, atp6SCAR and coxIISCAR markers appeared in both N and S cytoplasms when polymerase chain reaction (PCR) cycles prolonged more than 40 cycles. Since the reported molecular markers were dominant markers, the presence of the faint sterile-specific band in normal cytoplasm may lead to the mis-classification of pepper breeding lines. To solve this problem, one common forward primer and two different reverse primers specific to normal coxII and sterile orf456 genes were designed after analyzing their gene organizations. By using these three primers, N and S coxII specific bands were co-amplified in male-sterile lines, but only normal coxII specific band was amplified in maintainer lines. Since the reverse primer for sterile coxII was specifically designed 275 bp downstream of orf456, relatively stable PCR amplification patterns were observed regardless of the number of PCR cycles. These primer sets easily identified different mitotypes among the divergent breeding lines, commercial cultivars and diverse germplasms.

Fabrication and performance evaluation of ultraviolet photodetector based on organic /inorganic heterojunction

  • Abdel-Khalek, H.;El-Samahi, M.I.;Salam, Mohamed Abd-El;El-Mahalawy, Ahmed M.
    • Current Applied Physics
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    • v.18 no.12
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    • pp.1496-1506
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    • 2018
  • Organic/inorganic ultraviolet photodetector was fabricated using thermal evaporation technique. Organic/inorganic heterojunction based on thermally evaporated copper (II) acetylacetonate thin film of thickness 200 nm deposited on an n-type silicon substrate is introduced. I-V characteristics of the fabricated heterojunction were investigated under UV illumination of intensity $65mW/cm^2$. The diode parameters such as ideality factor, n, barrier height, ${\Phi}_B$, and reverse saturation current, $I_s$, were determined using thermionic emission theory. The series resistance of the fabricated diode was determined using modified Nord's method. The estimated values of series resistance and barrier height of the diode were about $0.33K{\Omega}$ and 0.72 eV, respectively. The fabricated photodetector exhibited a responsivity and specific detectivity about 9 mA/W and $4.6{\times}10^9$ Jones, respectively. The response behavior of the fabricated photodetector was analyzed through ON-OFF switching behavior. The estimated values of rise and fall time of the present architecture under UV illumination were about 199 ms and 154 ms, respectively. Finally, enhancing the photoresponsivity of the fabricated photodetector, post-deposition plasma treatment process was employed. A remarkable modification of the device performance was noticed as a result of plasma treatment. These modifications are representative in a decrease of series resistance and an increase of photoresponsivity and specific detectivity. The process of plasma treatment achieved an increment of external quantum efficiency from 5.53% to 8.34% at -3.5 V under UV illumination.

Implementation of RSA Exponentiator Based on Radix-$2^k$ Modular Multiplication Algorithm (Radix-$2^k$ 모듈라 곱셈 알고리즘 기반의 RSA 지수승 연산기 설계)

  • 권택원;최준림
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.12 no.2
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    • pp.35-44
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    • 2002
  • In this paper, an implementation method of RSA exponentiator based on Radix-$2^k$ modular multiplication algorithm is presented and verified. We use Booth receding algorithm to implement Radix-$2^k$ modular multiplication and implement radix-16 modular multiplier using 2K-byte memory and CSA(carry-save adder) array - with two full adder and three half adder delays. For high speed final addition we use a reduced carry generation and propagation scheme called pseudo carry look-ahead adder. Furthermore, the optimum value of the radix is presented through the trade-off between the operating frequency and the throughput for given Silicon technology. We have verified 1,024-bit RSA processor using Altera FPGA EP2K1500E device and Samsung 0.3$\mu\textrm{m}$ technology. In case of the radix-16 modular multiplication algorithm, (n+4+1)/4 clock cycles are needed and the 1,024-bit modular exponentiation is performed in 5.38ms at 50MHz.

Design of AC/DC Combined V2X System for Small Electric Vehicle (소형 전기차 적용을 위한 AC/DC 복합 V2X 시스템 설계)

  • Kim, Yeong-Jung;Chang, Young-Hag;Moon, Chae-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.17 no.4
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    • pp.617-624
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    • 2022
  • The small electric vehicles equipped with V2X(vehicle to everything) systems may provide more information and function to the existing navigation system of the vehicle. The key components of V2X technology include V2V (vehicle to vehicle), V2N(vehicle to network) and V2I (vehicle to infrastructure). This study is to design and implementation of VI type E-PTO which is interfaced with external equipments, the work designs the components of E-PTO such as DC/DC converter, DC/AC converter, battery bidirectional charging system etc. Also, it implements the devices and control systems for driving. The test results of VI type E-PTO components showed allowable 10% requirements of transient voltage variation rate and recovery time within 100ms for start/stop and normal operation.

Comparison analysis of YOLOv10 and existing object detection model performance

  • Joon-Yong Kim
    • Journal of the Korea Society of Computer and Information
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    • v.29 no.8
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    • pp.85-92
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    • 2024
  • In this paper presents a comparative analysis of the performance between the latest object detection model, YOLOv10, and its previous versions. YOLOv10 introduces NMS-Free training, an enhanced model architecture, and an efficiency-centric design, resulting in outstanding performance. Experimental results using the COCO dataset demonstrate that YOLOv10-N maintains high accuracy of 39.5% and low latency of 1.84ms, despite having only 2.3M parameters and 6.7G floating-point operations (FLOPs). The key performance metrics used include the number of model parameters, FLOPs, average precision (AP), and latency. The analysis confirms the effectiveness of YOLOv10 as a real-time object detection model across various applications. Future research directions include testing on diverse datasets, further model optimization, and expanding application scenarios. These efforts aim to further enhance YOLOv10's versatility and efficiency.

Detection of Hydrocarbons Induced by Electron Beam Irradiation of Almond (Prunus amygosalus L.) and Peanut (Arachis hypogaea) (전자선 조사한 아몬드(Prunus amygosalus L.)와 땅콩(Arachis hypogaea)에서 유래한 지방분해산물 분석)

  • Jeong, In Seon;Kim, Jae Sung;Hwang, In Min;Choi, Sung Hwa;Choi, Ji Yeon;Nho, Eun Yeong;Khan, Naeem;Kim, Byung Sook;Kim, Kyong Su
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.20-24
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    • 2013
  • Food irradiation has recently become one of the most successful techniques to preserve food with increased shelf life. This study aims to analyze hydrocarbons in almonds (Prunus amygosalus L.) and peanuts (Arachis hypogaea) induced by electron beam irradiation. The samples were irradiated at 0, 1, 3, 5 and 10 kGy by e-beam and using florisil column chromatography fat, and content was extracted. The induced hydrocarbons were identified using gas chromatography-mass spectrometry (GC/MS). The major hydrocarbons in both irradiated samples were 1,7-hexadecadiene ($C_{16:2}$) and 8-heptadecene ($C_{17:1}$) from oleic acid, 1,7,10-hexadecatriene ($C_{16:3}$) and 6,9-heptadecadiene ($C_{17:2}$) from linoleic acid and 1-tetradecene ($C_{14:1}$) and pentadecane ($C_{15:0}$) from palmitic acid. Concentrations of the hydrocarbons produced by e-beam were found to be depended upon the composition of fatty acid in both almonds and peanuts. The $C_{n-2}$ compound was found to be higher than $C_{n-1}$ compound in oleic acid and palmitic acid, while in case of linoleic acid, $C_{n-1}$compound was higher than $C_{n-2}$ compound. The radiation induced hydrocarbons were detected only in irradiated samples, with 1 kGy or above, and not in the non-irradiated ones. The production of 1,7-hexadecadiene ($C_{16:2}$), 8-heptadecene ($C_{17:1}$), 1,7,10-hexadecatriene ($C_{16:3}$) and 6,9-heptadecadiene ($C_{17:2}$), in high concentration gave enough information to suggest that these may be the possible marker compounds of electron beam irradiation in almonds and peanuts.

Analysis of Dual Phosphorylation of Hog1 MAP Kinase in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry

  • Choi, Min-Yeon;Kang, Gum-Yong;Hur, Jae-Young;Jung, Jin Woo;Kim, Kwang Pyo;Park, Sang-Hyun
    • Molecules and Cells
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    • v.26 no.2
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    • pp.200-205
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    • 2008
  • The mitogen-activated protein kinase (MAPK) signaling pathway is activated in response to extracellular stimuli and regulates various activities in eukaryotic cells. Following exposure to stimuli, MAPK is known to be activated via dual phosphorylation at a conserved TxY motif in the activation loop; both threonine and tyrosine residues are phosphorylated by an upstream kinase. However, the mechanism underlying dual phosphorylation is not clearly understood. In the budding yeast Saccharomyces cerevisiae, the Hog1 MAPK mediates the high-osmolarity glycerol (HOG) signaling pathway. Tandem mass spectrometry and phosphospecific immunoblotting were performed to quantitatively monitor the dynamic changes occurring in the phosphorylation status of the TxY motif of Hog1 on exposure to osmotic stress. The results of our study suggest that the tyrosine residue is preferentially and dynamically phosphorylated following stimulation, and this in turn leads to the dual phosphorylation. The tyrosine residue was hyperphosphorylated in the absence of a threonine residue; this result suggests that the threonine residue is critical for the control of signaling noise and adaptation to osmotic stress.

A numerical study on the characteristics of a thermal mass air flow sensor with periodic heating pulses (주기 발열 파형을 이용한 열식 질량 유량계의 특성에 관한 수치적 연구)

  • Jeon, Hong-Kyu;Oh, Dong-Wook;Park, Byung-Kyu;Lee, Joon-Sik
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2482-2487
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    • 2007
  • Numerical simulations are conducted for the analysis of a thermal mass air flow sensor with periodic heating pulses on silicon-nitride ($Si_3N_4$) thin membrane structure. This study aims to find the locations of temperature sensors on the thin membrane and the heating pulse conditions, that the higher sensitivity can be achieved, for the development of a MEMS fabricated mass air flow sensor which is driven in periodic heating pulse. The simulations, thus, focus on the membrane temperature profile according to variation of the flow velocity, heating duration time and imposed power. The flow velocity of the simulations is ranging from 3 m/s to 35 m/s, heating duration time from 1 ms to 3 ms and imposed power from 50 mW to 90 mW. The corresponding Reynolds numbers vary from 1000 to 10000.

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Superconducting Strip Ion Detectors for Time-of-flight Mass Spectrometer

  • Zen, N.;Suzuki, K.;Shiki, S.;Ukibe, M.;Koike, M.;Casaburi, A.;Ejrnaes, M.;Cristiano, R.;Ohkubo, M.
    • Progress in Superconductivity
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    • v.14 no.2
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    • pp.92-95
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    • 2012
  • Superconducting detectors are promising as ion detectors for time-of-flight mass spectrometers (TOF MS). They can achieve mass-independent detection efficiency even for macromolecular bombardments, because output signals are produced through the deposited kinetic energy at ion impact instead of secondary electron emission that is the ion detection mechanism of conventional microchannel plate (MCP) detectors or secondary electron multipliers (SEM). Among the superconducting detectors, the superconducting strip ion detectors (SSIDs), which consist of several hundreds of superconducting lines with a width of a few hundreds nm and a thickness of a few tens of nm, have a fast response time of less than 1 ns. Inherently, the response time of SSIDs is determined by kinetic inductance, so that it was difficult to realize a fast SSID with a large detection area. However, we succeeded in realizing the detector size up to $5{\times}5mm^2$ without response time degradation by using a parallel configuration.

Structural Identification of a Non-Glycosylated Variant at Ser126 for O-Glycosylation Site from EPO BRP, Human Recombinant Erythropoietin by LC/MS Analysis

  • Byeon, Jaehee;Lim, Yu-Ri;Kim, Hyong-Ha;Suh, Jung-Keun
    • Molecules and Cells
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    • v.38 no.6
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    • pp.496-505
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    • 2015
  • A variant peak was detected in the analysis of RP-HPLC of rHu-EPO, which has about 7% relative content. Fractions of the main and the variant peaks were pooled separately and further analyzed to identify the molecular structure of the variant peak. Total mass analysis for each peak fraction using ESI-TOF MS shows differences in molecular mass. The fraction of the main peak tends to result in higher molecular masses than the fraction of the variant. The detected masses for the variant are about 600-1000 Da smaller than those for the main peak. Peptide mapping analysis for each peak fraction using Asp-N and Glu-C shows differences in O-glycopeptide profiles at Ser126. The O-glycopeptides were not detected in the fraction of the variant. It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126.