• 대한융합한의학회지
• /
• 제1권1호
• /
• pp.17-24
• /
• 2021

Objectives: Excessive accumulation of fat on specific region, regarded as localized fat, is one of the serious problems and well-known risk factors of health. Recently, an interest in health and aesthetics is growing by treating lipolytic injection. Polygonatum sibiricum Rehd (PS) has been known to have anti-oxidant, -aging and -atherosclerotic effects. In this study, we investigated the lipolytic effects of PS pharmacopuncture in obese mice. Methods: Male C57BL/6J mice was fed with high fat diet to induce obesity for 12 weeks. PS pharmacopuncture was dissolved in saline by adjusting pH 7. 100 μL of PS pharmacopunture was injected subcutaneously into the left side inguinal fat pad, while saline was injected into the right side inguinal fat pad in mice as self-control. Samples were treated 3 times per weeks for 2 weeks. Results: PS pharmacopunture significantly decreased the inguinal fat weight compared to left side inguinal fat pad. Decrease rate of PS pharmacopuncture was about 21%. In addition, the diameter of adipocyte in inguinal fat tissues was significantly reduced by 17% compared to saline-injected side. There was no sign of toxicity through whole experiments. Conclusion: The present study indiates that PS pharmacopunture could be a material derived from natural herb as a lipolytic injection for decreasing localized fat.

• 한국자원식물학회지
• /
• 제33권6호
• /
• pp.604-614
• /
• 2020

Chronological aging and photoaging affect appearance, causing wrinkles, pigmentation, texture changes, and loss of elasticity in the skin. Phragmites communis is a tall perennial herb used for its high nutritional value and for medicinal purposes, such as relief from fever and vomiting and facilitation of diuresis. In this study, we investigated the effects of ethanol extract of P. communis rhizome (PCE) on skin aging. The total flavonoid and total phenolic content in PCE were 2.92 ± 0.007 ㎍ of quercetin equivalents (QE) and 231.8 ± 0.001 ㎍ of gallic acid equivalents (GAE) per 100 mg of dried extract (n = 3). The half-maximal inhibitory concentration (IC50) values of PCE for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and hydrogen peroxide scavenging activities were 0.96 and 0.97 mg/mL, respectively. PCE showed inhibitory effects on tyrosinase when L-tyrosine (IC50 = 1.25 mg/mL) and L-3,4-dihydroxyphenylalanine (IC50 = 0.92 mg/mL) were used as substrates. PCE treatment up to 200 ㎍/mL for 24 h did not cause any significant cytotoxicity in B16F10 melanocytes, human dermal fibroblasts (HDFs), and HaCaT keratinocytes. In B16F10 melanocytes, PCE (25 and 50 ㎍ /mL) inhibited melanin production and cellular tyrosinase activity after challenge with α-melanocyte-stimulating hormone (α-MSH; p < 0.05). In HDFs, PCE suppressed the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced the activity of elastase (p < 0.05). In addition, ultraviolet B (UVB)-mediated downregulation of hyaluronic acid synthase-2 gene expression in HaCaT keratinocytes was also effectively suppressed by PCE treatment. Overall, our results showed that PCE has potential anti-skin aging activity associated with the suppression of hyperpigmentation, wrinkle formation, and reduction in dryness. PCE is a promising candidate for the development of an anti-skin aging cosmetic ingredient.

• Tuberculosis and Respiratory Diseases
• /
• 제87권4호
• /
• pp.465-472
• /
• 2024

Background: Patients with chronic obstructive pulmonary disease (COPD) expressing eosinophilia experience slightly fewer episodes of community-acquired pneumonia (CAP), than those without eosinophilia. However, the severity and burden of hospitalized pneumonia patients with COPD involving eosinophilia have not been assessed. Methods: We evaluated the differences in clinical characteristics between patients with CAP and COPD with or without eosinophilia by a post hoc analysis of a prospective, multi-center, cohort study data. Results: Of 349 CAP patients with COPD, 45 (12.9%) had eosinophilia (blood eosinophil ≥300 cells/µL). Patients with eosinophilia had a lower sputum culture percentile (8.1% vs. 23.4%, p<0.05), a lower percentile of neutrophils (70.3% vs. 80.2%, p<0.05), reduced C-reactive protein levels (30.6 mg/L vs. 86.6 mg/L, p<0.05), and a lower pneumonia severity index score (82.5 vs. 90.0, p<0.05), than those without eosinophilia. The duration of antibiotic treatment (8.0 days vs. 10.0 days, p<0.05) and hospitalization (7.0 days vs. 9.0 days, p<0.05) were shorter in eosinophilic patients. The cost of medical care per day (256.4 US$ vs. 291.0 US$, p<0.05), cost for the medication (276.4 US$ vs. 349.9 US$, p<0.05), and cost for examination (685.5 US$ vs. 958.1 US$, p<0.05) were lower in patients with eosinophilia than those without eosinophilia. Conclusion: Eosinophilia serves as a favorable marker for the severity of pneumonia, health-care consumption, and cost of medical care in patients with CAP and COPD.

• 한국식품저장유통학회지
• /
• 제23권2호
• /
• pp.267-274
• /
• 2016

본 연구에서는 다양한 생리활성을 가진다고 알려져 있는 약용열매인 산수유, 오미자, 복분자 및 구기자 4종의 기능성식품 소재로 활용하고자 혼합비율별로 추출한 열수 추출물의 이화학적 특성 및 항산화 활성을 조사하였다. 약용열매 혼합비율에 따른 열수 추출물의 pH는 3.22~3.52로 산성을 나타내었으며, 당도는 M3이 $3.20^{\circ}Brix$로 다른 시료에 비해 낮았고 산도는 3.60~5.85%으로 오미자 혼합비율이 높은 구간에서 5.85%로 가장 높게 나타났다. 추출수율은 M2, M1, MS, M4 및 M3 순으로 나타났으며, 총 폴리페놀 및 플라보노이드 함량은 각각 14.54 및 5.65 g/100 g으로 복분자 혼합비율이 높은 추출물인 M3에서 가장 높은 값을 나타내었다. DPPH 및 ABTS radical 소거활성은 M3이 $250{\sim}1,000{\mu}g/mL$ 농도에서 25.80~86.09% 및 33.43~90.49%로 가장 높게 나타났다. FRAP 및 환원력은 M3가 $250{\sim}1,000{\mu}g/mL$ 농도에서 각각 $0.36{\sim}0.86{\mu}M$ 및 0.21~0.96으로 가장 우수하였으며, 모든 혼합 열수 추출물에서 농도 의존적으로 증가하였다.

• Preventive Nutrition and Food Science
• /
• 제20권3호
• /
• pp.176-182
• /
• 2015

Abalone protein was hydrolyzed by enzymatic hydrolysis and the optimal enzyme/substrate (E/S) ratios were determined. Abalone protein hydrolysates (APH) produced by Protamex at E/S ratio of 1:100 showed angiotensin I converting enzyme inhibitory activity with $IC_{50}$ of 0.46 mg/mL, and APH obtained by Flavourzyme at E/S ratio of 1:100 possessed the oxygen radical absorbance capacity value of $457.6{\mu}M$ trolox equivalent/mg sample. Flavourzyme abalone protein hydrolysates (FAPH) also exhibited $H_2O_2$ scavenging activity with $IC_{50}$ of 0.48 mg/mL and $Fe^{2+}$+ chelating activity with $IC_{50}$ of 2.26 mg/mL as well as high reducing power. FAPH significantly (P<0.05) protected $H_2O_2$-induced hepatic cell damage in cultured hepatocytes, and the cell viability was restored to 90.27% in the presence of FAPH. FAPH exhibited 46.20% intracellular ROS scavenging activity and 57.89% lipid peroxidation inhibition activity in cultured hepatocytes. Overall, APH may be useful as an ingredient for functional foods.

• 문화기술의 융합
• /
• 제4권4호
• /
• pp.295-300
• /
• 2018

절편에 영양적 가치와 약리적 효능이 있는 파래분말 첨가량(0~8%)을 달리하여 절편을 제조하고 관능검사 및 조직감 특성을 비교 검토 하엿다. 파래분말 첨가량을 달리한 절편의 색도중 명도 L값은 파래분말 첨가량이 증가할수록 감소하였으며, 적색도 a값은 첨가량이 많아 질수록 유의적인 값을 보였다(p<0.05). 황색도(b값)는 파래분말을 첨가한 절편이 무첨가군보다는 높은 점수를 보였으며, 파래분말 첨가량이 증가할수록 황색도가 낮아져 절편의 녹색이 강하게 나타났다. 텍스쳐 특성에서는 경도, 응집성, 검성은 파래분말 첨가량이 증가할수록 증가하였으며 유의적인 차이를 나타내었다(p<0.05). 전반적인 기호도에서는 4%를 첨가한 파래절편이 가장 좋게 평가되었다.

• 한국식품영양학회지
• /
• 제35권5호
• /
• pp.359-368
• /
• 2022

In this study, the quality characteristics of kimchi, such as its salinity, pH, and acidity, were measured and compared, and the HT-29 human colon cancer cells were used to show the anticancer effects of kimchi. The kimchi samples used herein included standard kimchi (SK), turnip kimchi (TK), and turnip-powder-added kimchi (TPK). The measured pH and acidity of TK and TPK showed no significant differences with those of SK. Compared to SK and TK, TPK had higher DPPH scavenging activity and higher total flavonoid content, confirming its antioxidant activity. The cancer cell growth inhibition rates of TK and TPK were significantly higher than that of SK. In HT-29 cells treated with TPK, the mRNA expression of Bcl-xL, an anti-apoptosis-related gene, was lower, and the mRNA expressions of the apoptosis-related genes Bax, Bad, and caspase-9 were higher. TPK showed significantly higher levels of mRNA expressions for the cell-cycle-related genes p53 and p21 than the other samples, in addition to suppression effects on cancer cell proliferation. Compared to SK, TK and TPK suppressed the growth of colon cancer cells and showed higher anticancer effects. Therefore, it is shown that kimchi with added turnip powder had high anticancer effects.

• Journal of Animal Science and Technology
• /
• 제58권8호
• /
• pp.29.1-29.10
• /
• 2016

Background: It is known that large dogs who are fed lamb and rice diets are at increased risk to develop taurine-deficiency-induced dilated cardiomyopathy. Since dogs obligatorily conjugate bile acids (BA) with taurine, we determined whether rice bran (RB) or other fibers (cellulose; CL, beet pulp; BP) would affect BA excretion and/or the taurine status of dogs. Results: Eighteen medium/large mixed-breed dogs were given purified diets containing CL, BP, or RB for 12 weeks. Taurine concentrations in plasma and whole blood were significantly decreased at week 12. The BP group, compared to the CL or RB groups, showed significantly lower taurine concentrations in plasma ($6.5{\pm}0.5$ vs $20.4{\pm}3.9$ and $13.1{\pm}2.0{\mu}mol/L$, respectively, P < 0.01, $mean{\pm}SEM$) and in whole blood ($79{\pm}10$ vs $143{\pm}14$ and $127{\pm}14{\mu}mol/L$, respectively, P < 0.01), lower apparent protein digestibility ($81.9{\pm}0.6$ vs $88.8{\pm}0.6$ and $88.1{\pm}1.2%$, respectively, P < 0.01), and higher BA excretions ($5.6{\pm}0.1$ vs $3.4{\pm}0.5$ and $3.4{\pm}0.4{\mu}mol/g$ feces, respectively, P < 0.05) at week 12. Conclusions: These results do not support the hypothesis that RB is likely to be a primary cause of lamb meal and rice diets, increasing the risk of taurine deficiency in large dogs. However these indicate that BP may contribute to a decrease taurine status in dogs by increasing excretion of fecal BA and decreasing protein digestibility, thus decreasing the bioavailability of sulfur amino acids, the precursors of taurine.

• 한국습지학회지
• /
• 제20권3호
• /
• pp.249-255
• /
• 2018

침입외래식물인 단풍잎돼지풀(Ambrosia trifida L.)이 자생식물의 종수와 고유종인 층층둥굴레(Polygonatum stenophyllum Maxim.)의 활력도에 미치는 영향을 알아보고자 하였다. 단풍잎돼지풀의 식피율 구배에 따라서 자생식물 종수, 층층둥굴레의 사망률, 영양생장과 생식생장 등의 변화를 2015년 6월부터 2016년 10월까지 관찰하고 분석하였다. 단풍잎돼지풀의 식피율 처리는 각각 100% 제거, 40~60% 제거, 비제거구(대조구)로 하였다. 그 결과 단풍잎돼지풀의 식피율을 40% 이상 제거할 때, 출현한 식물 종수와 층층둥글레의 개체수 그리고 영양과 번식측정 항목의 활력도가 높았다. 이를 통해서 단풍잎돼지풀의 식피율을 40% 이상을 제거하는 것은 연구 지역의 식물종다양성과 준위협종인 층층둥굴레의 개체군 보전에 효과적이라고 판단된다.

• KSBB Journal
• /
• 제29권4호
• /
• pp.244-249
• /
• 2014

Biosynthesis pathway of medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acid ${\beta}$-oxidation pathway was constructed in recombinant Escherichia coli by introducing the Pseudomonas sp. 61-3 PHA synthase gene (phaC2) and the maoC genes from Pseudomonas putida, Sinorhizobium meliloti, and Ralstonia eutropha. The metabolic link between fatty acid ${\beta}$-oxidation pathway and PHA biosynthesis pathway was constructed by MaoC, which is homologous to P. aeruginosa (R)-specific enoyl-CoA hydratase (PhaJ1). When the E. coli W3110 strains expressing the phaC2 gene and one of the maoC genes from P. putida, Sinorhizobium meliloti, and Ralstonia eutropha were cultured in LB medium containing 2 g/L of sodium decanoate as a carbon source, MCL-PHA that mainly consists of 3-hydroxyhexanoate (3HHx), 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), was produced. The monomer composition of PHA and PHA contents varied depending on MaoC employed for the production of PHA. The highest PHA content of 18.7 wt% was achieved in recombinant E. coli W3110 expressing the phaC2 gene and the P. putida maoC gene. These results suggest that MCL-PHA biosynthesis pathway can be constructed in recombinant E. coli strains from the b-oxidation pathway by employing MaoC able to supply (R)-3-hydroxyacyl-CoA, the substrate of PHA synthase.