• Title/Summary/Keyword: $K_m$ value

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A Rapid COD Test Procedure for Alkaline Permanganate Value Determination (알칼리성 $100^{\circ}C$에서 화학적 산소요구량 신속정량법)

  • Czae, Myung-Zoon;Ly, Suw-Young;Kim, Mi-Kyung
    • Analytical Science and Technology
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    • v.8 no.2
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    • pp.167-170
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    • 1995
  • A simple and rapid permanganate value test procedure for the estimation of COD, based on the photometric measurement of the remained permanganate after the color bleaching by oxygen demand organics during digestion, has been described. Optimized conditions were 1% NaOH, 0.6mM $KMnO_4$, with a closed reflux in the boiling water bath for 90 sec using sample size of 5mL.

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Anti-Proliferation Effects of Benzimidazole Derivatives on HCT-116 Colon Cancer and MCF-7 Breast Cancer Cell Lines

  • Al-Douh, Mohammed Hadi;Sahib, Hayder B.;Osman, Hasnah;Hamid, Shafida Abd;Salhimi, Salizawati M.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.8
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    • pp.4075-4079
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    • 2012
  • Benzimidazoles 1-4 were obtained using modified synthesis methods and studied for their ability to inhibit cell proliferation of colon cancer cell HCT-116 and breast cancer cell MCF-7 using MTT assays. In the HCT-116 cell line, benzimidazole 2 was found to have an $IC_{50}$ value of $16.2{\pm}3.85{\mu}g/mL$ and benzimidazole 1 a value of $28.5{\pm}2.91{\mu}g/mL$, while that for benzimidazole 4 was $24.08{\pm}0.31{\mu}g/mL$. In the MCF-7 cell line, benzimidazole 4 had an $IC_{50}$ value of $8.86{\pm}1.10{\mu}g/mL$, benzimidazole 2 a value of $30.29{\pm}6.39{\mu}g/mL$, and benzimidazole 1 a value of $31.2{\pm}4.49{\mu}g/mL$. Benzimidazole 3 exerted no cytotoxity in either of the cell lines, with $IC_{50}$ values $>50{\mu}g/mL$. The results suggest that benzimidazoles derivatives may have chemotherapeutic potential for treatment of both colon and breast cancers.

Performance Measurement of Diagnostic X Ray System (진단용 X선 발생장치의 성능 측정)

  • You, Ingyu;Lim, Cheonghwan;Lee, Sangho;Lee, Mankoo
    • Journal of the Korean Society of Radiology
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    • v.6 no.6
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    • pp.447-454
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    • 2012
  • To examine the performance of a diagnostic X-ray system, we tested a linearity, reproducibility, and Half Value Layer(HVL). The linearity was examined 4 times of irradiation with a given condition, and we recorded a level of radiation. We then calculated the mR/mAs. And the measured value should not be more than 0.1. If the measured value was more than 0.1, we could know that the linearity was decreased. The reproducibility was analyzed 10 times of irradiations at 80kVp, 200mA, 20mAs and 120kVp, 300mA, 8mAs. The values from these analyses were integrated into CV equation, and we could get outputs. The reproducibility was good if the output was lower than 0.05. HVL was measured 3 times of irradiation without a filter, and we inserted additional HLV filters with 0, 1, 2, 4 mm of thickness. We tested the values until we get the measured value less than a half of the value measured without additional filter. We tested the linearity, the reproducibility, and HVL of 5 diagnostic X-ray generators in this facilities. The linearity of No. 1 and No. 5 generator didn't satisfy the standard for radiation safety around 300mA~400mA and 100mA~200mA, respectively. HVL of No.1 generator was not satisfied at 80kVp. The outputs were higher in the three-phase equipment than the single-phase equipment. The old generators need to maintain and exchange of components based on the these results. Then, we could contribute to getting more exact diagnosis increasing a quality of the image and decreasing an expose dose of radiation.

Zeros and Uniqueness of Difference Polynomials of Meromorphic Functions

  • Qi, Xiaoguang;Dou, Jia
    • Kyungpook Mathematical Journal
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    • v.53 no.4
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    • pp.541-552
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    • 2013
  • This research is a continuation of a recent paper due to the first author in [9]. Different from previous results, we investigate the value distribution of difference polynomials of moromorphic functions in this paper. In particular, we are interested in the existence of zeros of $f(z)^n({\lambda}f(z+c)^m+{\mu}f(z)^m)-a$, where f is a moromorphic function, n, m are two non-negative integers, and ${\lambda}$, ${\mu}$ are non-zero complex numbers. However, the proof here is obviously different to the one in [9]. We also study difference polynomials of entire functions sharing a common value, which improves the result in [10, 13].

Contents of Total Flavonoid and Biological Activities of Edible Plants (식물성 식품중 총플라보노이드 함량과 생리활성 탐색)

  • Son, Eun-Shim;Oh, Sang-Suk;Han, Dae-Suk;Lee, Jong-Mee
    • Journal of the Korean Society of Food Culture
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    • v.16 no.5
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    • pp.504-514
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    • 2001
  • The contents of total flavonoids, total antioxidant status and superoxide scavenger activity of edible plants were measured. Among twenty one samples of leafy vegetables and teas, mugwort (Artemesia capillaris) and green teas gave high total flavonoids contents, 48.2 mg/g sample and 44.7 mg/g sample, respectively. The highest concentration of total flavonoids among eighty nine samples analyzed was 52.1 mg/g sample from propolis. When samples were tested for total antioxidant status, a kind of groundsel showed the highest Total Antioxidant Status(TAS) value, 13.4 mM among leafy vegetables and teas and green tea gave high TAS value, 11.84 mM. On the other hand, purslane gave the lowest TAS value, 0.01 mM. The superoxide scavenge activity was measured from the extracts of all edible plants. The superoxide scavenge activity of polygonatum(81.1%) and chrysantemum (78.5%) were the highest among leafy vegetables, teas and medicinalplants. On the other hand, ginseng, poria, cassiae semen and dioscoreae rhizoma showed no superoxide scavenge activities.

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Studies on Hematologic Values and Blood Chemistry Values of Normal Jindo Dogs 3. Serum Electrolyte Values for Adult Jindo Dogs (진도견(珍島犬)의 혈액상(血液像) 및 혈액화학치(血液化學値)에 관(關)한 연구(硏究) 제삼보(第三報) 진도견(珍島犬)의 혈청전해질치(血淸電解質値))

  • Park, Nam-Yong;Rim, Bong-Ho;Cho, Sung-Man
    • Korean Journal of Veterinary Research
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    • v.20 no.2
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    • pp.175-178
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    • 1980
  • Normal values of serum electrolytes were determined for adult 36 females and 18 males, 1 to 6 years old, of Jindo dogs. The mean values, standard deviation and rangEs of sodium, potassium, chloride, calcium and inorganic phosphorus were studied and data analyzed to determine whether differences existed between the values of female and those of male dogs. The results obtained in this study were as follows: 1. The mean value of serum sodium was $143{\pm}1.5mEq/liter$, ranged from 138mEq/liter to 152 mEq/liter. The mean values for the females and males were $144{\pm}2.0mEq/liter$ and $142{\pm}1.7mEq/liter$, respectively. They showed that the female dogs had a tendency toward higher values than the male dogs, but no significant sex-related differences were observed. 2. The mean value of serum potassium was $4.6{\pm}0.2mEq/liter$, ranged frem 3.5mEq/liter to 6.0mEq/liter. The mean values for the females and males were $4.7{\pm}0.4mEq/liter$ and $4.5{\pm}0.3mEq/liter$. They showed that the female dogs had a tendency toward higher values than the male dogs, but sex differences were not found to be significant. 3. The mean value of serum chloride was $103{\pm}3.0mEq/liter$ ranged from 95mEq/liter to 117mEq/liter. The mean values for the females and males were $103{\pm}3.2mEq/liter$ and $103{\pm}3.3mEq/liter$, no sex differences showed. 4. The mean value of serum calcium was $5.2{\pm}0.1mEq/liter$ ranged from 4.7mEq/liter to 5.8mEq/liter. The mean values for the females and males were $5.4{\pm}0.1mEq/liter$ and $5.2{\pm}0.09mEq/liter$ respectively, no significant sexd iffere-nces were observed. 5. The mean value of serum inorganic phosphorus was $4.3{\pm}0.7mg/100ml$, ranged from 2.5mg/100ml to 5.8mg/100ml. The mean values for the females and males were $4.3{\pm}0.9mg/100ml$ and $4.1{\pm}0.8mg/100ml$, respectively, no sex differences showed.

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Effect of Pyrimidylsalicylate on the Valine Sensitive Acetolactate Synthase Purified from Serroatia marcescens

  • Yang, Jeong-Hee;Kim, Soung-Soo
    • BMB Reports
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    • v.30 no.1
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    • pp.13-17
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    • 1997
  • The inhibitory effect of herbicides such as sulfonylurea derivatives, imidazolinones and pyrimidylsalicylate has been examined on the purified valine sensitive acetolactate synthase (ALS) from Serratia marcescens. The concentration of sulfometuron methyl which inhibits 50% of the ALS activity was 2.5 mM. The required concentrations of triasulfuron, primisulfuron methyl and imazaquin for the 50% inhibition of the ALS activity were 1 mM. The resistance of Serratia ALS to sulfometuron methyl, imazapyr and imazaquin is similar to that of E. coli ALS 1. However, pyrimidylsalicylate showed a potent inhibitory effect on the Serratia ALS almost 13 times more potent than on E. coli ALS II, which is known as herbicide-sensitive isozyme. The inhibitory mode was competitive against pyruvate. 150 value was determined to be $17{\mu}M$ in an assay mixture containing 20 mM pyruvate, and the $K_1$, value was calculated to be $0.4{\mu}m$ from the modified double reciprocal plot of 1/V versus $1/S^2$.

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Background Level of Atmospheric Radon-222 Concentrations at Gosan Station, Jeju Island, Korea in 2011

  • Kim, Won-Hyung;Ko, Hee-Jung;Hu, Chul-Goo;Lee, Haeyoung;Lee, Chulkyu;Chambers, S.;Williams, A.G.;Kang, Chang-Hee
    • Bulletin of the Korean Chemical Society
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    • v.35 no.4
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    • pp.1149-1153
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    • 2014
  • Real-time monitoring of hourly atmospheric radon (Rn-222) concentration was performed throughout 2011 at Gosan station, Jeju Island, one of the least polluted regions in Korea, in order to characterize the background levels, and temporal variations on diurnal to seasonal time-scales. The annual mean radon concentration for 2011 was $2527{\pm}1356$ mBq $m^{-3}$, and the seasonal cycle was characterized by a broad winter maximum, and narrow summer minimum. Mean monthly radon concentrations, in descending order of magnitude, were Oct > Sep > Feb > Nov > Jan > Dec > Mar > Aug > Apr > Jun > May > Jul. The maximum monthly mean value (3595 mBq $m^{-3}$, October), exceeded the minimum value (1243 mBq $m^{-3}$, July), by almost a factor of three. Diurnal composite hourly concentrations increased throughout the night to reach their maximum (2956 mBq $m^{-3}$) at around 7 a.m., after which they decreased to their minimum value (2259 mBq $m^{-3}$) at around 3 p.m. Back trajectory analyses indicated that the highest radon events typically exhibited long-term continental fetch over Asia before arriving at Jeju. In contrast, low radon events were generally correlated with air mass fetch over the North Pacific Ocean. Radon concentrations typical of predominantly continental, and predominantly oceanic fetch, differed by a factor of 3.8.

Aberrant Expression of E-cadherin in Lung Tissues of Patients with Probable Lung Cancer

  • Yuan, Yu-Lin;Wang, Yu-Ming;Liu, Hua;Qin, Gui-Fang;Tang, Ai-Guo;Duan, Yong
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.5149-5153
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    • 2012
  • Introduction: This study assessed the relationship of E-cadherin mRNA and protein expression with the diagnosis of lung cancer with the aim of providing an auxiliary diagnostic method. Methods: Semi-quantitative nested RT-PCR and western blotting were applied to detect E-cadherin mRNA transcripts and protein, respectively, in 30 cases of diagnostic lung cancer, 30 cases of clinically suspected patients with lung cancer and 30 cases of other disease. Immunohistochemical staining was also used to detect E-cadherin. Results: Remarkably decreased levels of relative E-cadherin mRNA value and increased E-cadherin protein negativity were observed in probable lung cancer, when compared with possible lung cancer and others. With a threshold of 1.45, relative E-cadherin mRNA value showed a sensitivity of 90% and a specifity of 83% for the diagnosis of lung cancer. The combination of decreased relative E-cadherin mRNA value and negative E-cadherin protein increased the specificity and sensitivity. Conclusion: These data suggest that Chinese patients with diagnostic lung cancer have similar decreased levels of relative E-cadherin mRNA and E-cadherin protein value in the lung cancer tissues as in lung cancer patients in other countries. Measurement of relative E-cadherin mRNA and protein values in lung cancer tissues has potential for lung cancer diagnosis.

Effect of Carbon on Microstructure and Texture in Low Carbon Steels (저탄소강의 미세조직과 집합조직에 대한 탄소의 영향)

  • Jeong, Woo Chang
    • Journal of the Korean Society for Heat Treatment
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    • v.27 no.2
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    • pp.79-89
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    • 2014
  • The effect of carbon on the microstructure and texture of low carbon steels was investigated in a series of 1.6 Mn-0.3Cr-0.2Mo-0.001B steels with carbon ranging from 0.021 to 0.048%. Intensity of {111} orientation increased with decreasing the carbon content, resulting in the increase in $r_m$ value. The highest $r_m$ value of 1.30 was obtained in 0.021%C steel annealed at $820{\sim}850^{\circ}C$ according to the typical galvannealing heat cycle. Martensite volume fraction was not substantially affected by the annealing temperature. It was found that the fine and uniformly distributed martensite particles which were present in amounts of about 5% volume fraction were desirable for the highest $r_m$ value. The other factor affecting the high $r_m$ value was the preferred epitaxial growth of retained ferrite with {111} orientation into austenite during cooling.