• Title/Summary/Keyword: $J_e$

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Binding of the His-tagged Tail Protein J of Bacteriophage Lambda with Escherichia coli K-12 (히스티딘으로 표지된 람다 박테리오파아지 꼬리 단백질 J와 대장균 K-12와의 결합)

  • Shin, Hae Ja
    • Journal of Life Science
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    • v.28 no.1
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    • pp.78-82
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    • 2018

  • Detection of pathogenic microorganisms takes several days by conventional methods. It is necessary to assess microorganisms in a timely manner to reduce the risk of spreading infection. For this purpose, bacteriophages are chosen for use as a biosensing tool due to their host specificity, wide abundance, and safety. However, their lytic cycle limits their efficacy as biosensors. Phage proteins involved in binding to bacteria could be a robust alternative in resolving this drawback. Here, a fragment of tail protein J (residues 784 to 1,132) of phage lambda fused with 6X His-tag (6HN-J) at its N-terminus was cloned, overexpressed, purified, and characterized for its binding with microorganisms. The purified protein demonstrated a size of about 38 kDa in sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and bound with anti-His monoclonal antibodies. It bound specifically to Escherichia coli K-12, and not Salmonella typhimurium, Bacillus subtilis, or Pseudomonas aeruginosa in dot blotting. Binding of the protein to E. coli K-12 inhibited about 50% of the in vivo adsorption of the phage lambda to host cells at a concentration of $1{\mu}g/ml$ 6HN-J protein and almost 100% at $25{\mu}g/ml$ 6HN-J. The results suggest that a fusion viral protein could be utilized as a biosensing element (e.g., protein chips) for detecting microorganisms in real time.

  • https://doi.org/10.5352/JLS.2018.28.1.78 인용 PDF KSCI

A Marriage Problem Algorithm Based on Duplicated Sum of Inter-Preference Moving Method (중복된 최소 상호-호감도 합 이동방법을 적용한 결혼문제 알고리즘)

  • Lee, Sang-Un
    • Journal of the Korea Society of Computer and Information
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    • v.20 no.5
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    • pp.107-112
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    • 2015

  • This paper proposes a simplified algorithm devised to obtain optimal solution to the marriage problem. In solving this problem, the most widely resorted to is the Gale-Shapley algorithm with the time complexity of $O({\mid}V{\mid}^2{\mid}E{\mid})$. The proposed algorithm on the other hand firstly constructs a $p_{ij}$ matrix of inter-preference sum both sexes' preference over the opposite sex. Secondly, it selects $_{min}p_i$ from each row to establish ${\mid}p_{.j}{\mid}{\geq}2,j{\in}S$, ${\mid}p_{.j}{\mid}=1$, $j{\in}H$, ${\mid}p_{.j}{\mid}=0$, $j{\in}T$. Finally, it shifts $_{min}\{_{min}p_{ST},p_{SH}+p_{HT\}$ for $_{min}P_{ST}$ of $S{\rightarrow}T$ and $p_{SH}+p_{HT}$, $p_{HT}<_{min}p_{ST}$ of $S{\rightarrow}H$, $H{\rightarrow}T$. The proposed algorithm has not only improved the Gale-Shapley's algorithm's complexity of $O({\mid}V{\mid}^2{\mid}E{\mid})$ to $O({\mid}V{\mid}^2)$ but also proved its extendable use on unbalanced marriage problems.

  • https://doi.org/10.9708/jksci.2015.20.5.107 인용 PDF KSCI

The e-Logistics Integrated Platform for a Logistics Services Industry

  • Park H. J.;Kim Y. H.;Lee Y. J.
    • Proceedings of the KSRS Conference
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    • 2004.10a
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    • pp.351-354
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    • 2004

  • The concept of e-Business has been around for more than two decades but it has not been used widely enough due to high installation and maintenance cost and complexity. In order to solve such problems, two standardization organizations UN/CEFACT and OASIS have developed an open e-Business standard called ebXML that can be easily adopted by industries. Based on the ebXML, we have developed an e-Logistics integrated platform for industries to do business electronically. The e-Logistics integrated platform was applied to B2B postal logistics industry and efficient interoperability between companies with different systems has been achieved.

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Breakdown Characteristic of Transformer Oil Depending on Tip Radius (침전극 곡률 반경에 따른 절연유의 절연파괴 특성)

  • Lee, J.S.;Jeong, S.H.;Lee, H.K.;Lim, K.J.;Kim, H.H.
    • Proceedings of the KIEE Conference
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    • 1997.07d
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    • pp.1478-1480
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    • 1997

  • We investigated the breakdown characteristic of mineral oil according to applied voltage and tip radius. In this experiment, electrode system was point-plane geometry. The tip radius of needle was 5, 10, 20 and $25{\mu}m$, respectively. Applied voltage was AC and DC. We measured breakdown voltage for each tip radius with increasing electrode gap, 2mm to 10mm. Under nonuniform electric field, breakdown strength was higher when needle was negative than when needle was positive. Because it is polarity effects due to space charge. And the more sharp tip radius, whether we applied AC or DC, the higher breakdown strength. As tip radius increase, breakdown strength decreases exponentially.

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AVR controller design of synchronous generator (동기발전기의 자동전압조정장치 제어기 설계)

  • Lee, J.M.;Kim, M.H.;Lee, J.H.;Park, Y.H.;Lim, I.H.;Goo, K.M.
    • Proceedings of the KIEE Conference
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    • 1993.07a
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    • pp.167-170
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    • 1993

  • This paper deals with AVR(Automatic Voltage Regulator) control1or design of synchronous generator adapting AC indirect excitation system. The simulation results are presented in frequency and tine domain for two plants(A and B). Try and error method for compensator design is used.

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