• Archives of Pharmacal Research
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• 제17권5호
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• pp.354-359
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• 1994

The activity of SD-framesylcysteine O-methyltransferase was assayed by incubating the enzyrne with a synthetic in vitro substrate, [N-acetyl-S-trans, trns-famesyl-L-cysteine (AFC)], together with S-adenosyl-L-[emthyl-$_{14}$C)ester(AFCME)], was then analyzed either directly on HPLC or by converting the AFC[$methyl^{14}C$]ME to [$methyl^{14}C$] aclcohol by basehydrolysis. Employing these two analytical methods, it was established that a peptide purifed from rat liver cytosol fraction [Int. J. Biochem., 25, 1157 919930] strongly inhibited the above enzyme activity with $IC_{50}\; of\; 7.1\times 10^{-8}$ M. Also, the S-famesylcysteine O-methyltransferase from several human colon cancer cells was equally inhibited by the peptide.

• 제어로봇시스템학회논문지
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• 제10권1호
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• pp.54-59
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• 2004

We suggest a 3D vision inspection algorithm which is based on the external shape feature. Because many electronic parts have the regular shape, if we have the database of pattern and can recognize the object using the database of the object s pattern, we can inspect many types of electronic parts. Our proposed algorithm uses the geometrical pattern matching method and 3D database on the electronic parts. We applied our suggested algorithm fer inspecting several objects including typical IC and capacitor. Through the experiments, we could find that our suggested algorithm is more effective and more robust to the inspection environment(rotation angle, light source, etc.) than conventional 2D inspection methods. We also compared our suggested algorithm with the feature space trajectory method.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.148-148
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• 1993

본 실험에서는 유전자 재조합으로 제조한 [$^{125}$ I]-labeled human GM-CSF을 사용하여 GM-CSF의 HL-60 (promyelocytic leukemic cell)의 표면에 존재하는 GM-CSF의 수용체의 특성을 알고 GM-CSF의 수용체에 어떤 parameter로 결합하는지를 밝히고 나아가 현재 사용되고 있는 유전자 재조합으로 제조된 Prokine(Sargramostim)과 Lucky에서 제조된 GM-CSF (LDB-005)의 수용체에 대한 결합율을 측정, 비교하고자 하였다. 본 실험의 결과를 보면 유전자 재조립으로 제조된 Human[$^{125}$ I] GM-CSF가 HL-60 cell에 대하여 선택적으로 결합하고 표면수용체에 saturable하게 결합함을 알 수 있었으며 scatchard analysis 결과한 종류의 GM-CSF의 K3값은 2.03$\times$$10^{9}$/M로($IC_{50}$/=~493pM) 세포당 결합부위의 수는 75개 정도로 J. DiPersio et al과 Linda S. Park et al.의 보고와 비슷한 결과를 얻었다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.61-61
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• 1995

1993년 국내토양으로부터 분리동정된 Streptomyces속 균주중 항종양성이 우수한 균주 3주(DKM104, DKM117, DKM409)를 선정하고 이들을 대량 배양하여 종양억제인자를 분리하고 시험관내 종양세포 독성능 및 생체내 항암활성능과 이들 물질생산과 PLASMID DNA와의 관련성, $LD_{50}$등을 시험하여 새로운 항종양물질의 개발을 목적으로 하였다. 분리선별균주의 배양조건을 확립하였으며 배양여액을 국성이 적은 유기용매로부터 큰쪽으로 단계적으로 유효성분을 추출하여 Gel Chromatography를 이용하여 유효성분을 분획하였다. 시험관내 항종양능 시험은 MTT colorimetric검정법을 이용하여 $IC_{50}$값을 산정하였으며 생체내 항종양능은 마국의 NIC에서 권장하는 tumor panel system에 따랐다. 선정된 균주의 plasmid 분리는 alkalin lysis법을 채택하였으며 agarose gel electrophoresis로 plasmid profile을 시험하였다. Novobiocin등을 이용하여 Curing test를 시행하였고 독성실험은 $LD_{50}$량을 구해 항암효과 측정시에 Maximum dost로 투여하였고 최고용량을 기준으로 일정한 공비를 적응하여 3단계의 투여량을 설정하였다.(중략)

• Journal of Biosystems Engineering
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• 제30권2호
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• pp.114-120
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• 2005

In this study, a biological reaction and measurement control system was developed to rapidly measure the insecticide imidacloprid residues in agricultural products. The biological reaction part of the system was designed to include micro-pumps and valves for fluid transport, and a polystyrene covet as a reaction chamber. The measurement control part of the system consisted of a photodiode with a light-emitting diode for optical density measurement, and a control microcomputer to implement assay. Signal output was read as the rate of change in optical density at 645 nm. The sensitivity of the system was 2.2 ng/mL ($IC_50$). The system could execute a measurement cycle in about 19 minutes. Research will be continued to develop an automatic sampler fur imidacloprid residues from agricultural products.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.458-462
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• 2001

The localization of paclitaxel was investigated in suspension culture cells of Taxus chinensis. Over 93% of the cell-associated paclitaxel were detected throughout the entire culture period. Intracellular localization of paclitaxel over the culture time was analyzed further by cell fractionation for days 21 and 42. Paclitaxel contents in intracellular organelles were decreased at day 42, while the content in the cell wall fraction was increased at day 42 compared to the value for day 21. The localization of paclitaxel in the cell wall was confirmed by using the immunocytochemical method with the aid of a confocal laser scanning microscope.

• 한국주조공학회지
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• 제12권1호
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• pp.32-39
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• 1992

In this study, electron microscopy, Fractography and $J_{IC}$ test have been used to investigate the fracture behaviour in the scope of aging temperature which 7075 Al-alloy can have high strength. Conclusions obtained on this study are as followed. 1) When the 7075 Al-alloy was aged for 24hrs at $120^{\circ}C$, it's mechanical properties was excellent but fracture toughness decrement resulted from intergranular fracture was emerged. 2) The state of aged for 24hrs at $100^{\circ}C$, had the highest fracture toughess. 3) We could infer that intergranular fracture occurred because preferrential precpitated precipitates in the grain boundary growed in the form of colinear along the grain boundary.

• 한국해양공학회지
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• 제1권2호
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• pp.101-112
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• 1987

Fracture toughness characterization in the transition region is examined for heat-treated and ionnitrided Ni-Cr-Mo steel. After heat treatment for the specimens of Ni-Cr-Mo steel, organizations of specimens-specimens which are heat-treated and ion-nitrided for 4 hours at 500 .deg. C and 5 torr in 25%N/dub 2/-75%H/sub 2/mixed gas-, hardness variety, and X-ray diffraction pattern of the ion-nitriding compound layer are observed. Fracture toughenss test of unloading compliance method were conducted over the regions from room trmperature to -70.deg. C. The compound layer was consisted of r'=Fe/sub 4/N phase and ion-nitrided layer's depth was 200mm from surface. The transition regions of heat-treated and ion-nitrided specimens were about -30.deg. C and -50.deg. C, respectively. The transition region of ion-nitrided specimens is estimated less than that of heat-treated one, and this is the effect of ion-nitriding.

• 전자통신동향분석
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• 제4권2호
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• pp.22-42
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• 1989

세계 정보통신산업의 최근 큰 변화가 있었는데, 무역정책측면에서는 미국의 종합무역법확정 및 발효, 지역주의의 확대가 특징적으로 나타났으며 기술개발측면은 신기술 개발촉진, 국제간 생산 이전가속, 지적소유권 문제의 확산, 그리고 국제조직측면으로는 전기통신의 표준화, 신기술의 국제적 표준화, 국제 ISDN서비스 논의의 진전등이 환경변화 요인으로 지적되고 있다. 정보통신산업의 산업별 성장은 전체적으로 1987년이후 증가속도가 둔화되고 있으며, 특히 통신기기와 컴퓨터분야의 성장율이 저하되고 있다. 그러나 부품산업은 IC와 반도체의 수요확대에 힘입어 평균수준을 기록하고 있다. 국가별로 볼때 특징적인 것은 미국, 일본, 유럽 등 주요 선진국들에 비해 한국, 대만 등 신흥 공업국들의 성장속도가 빠르게 진행되고 있으며 시장점유율도 두드러지게 확대되고 있는 것으로 분석되었다.

• 제어로봇시스템학회논문지
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• 제16권2호
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• pp.172-176
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• 2010

Recently, LCD (Liquid Crystal Display) requires various technical challenges; high definition, high quality, big size, and low price. These demands more pixels in the fixed area of the LCD and very fine lead pitch of the driving IC which controls the pixels. Therefore, a new packaging technology is needed to meet such technical requirement. NCP (Non Conductive Paste) is one of the new packaging methods and has excellent characteristics to overcome the problems of the ACF (Anisotropic Conductive Film). In this paper, we analyzed the process of the NCP for COF (Chip on FPCB) and proposed the key design parameters of the NCP process. Through a series of experiments, we obtained the stable values of the design parameters for successful NCP process.