• 한국균학회지
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• 제45권2호
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• pp.121-131
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• 2017

본 연구에서는 참나무 수종을 달리하여 톱밥재배한 표고의 항산화 활성을 알아보기 위해 건조된 표고를 70% methanol로 추출하여 총 폴리페놀 함량, 총 플라보노이드 함량, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거활성, ferric reducing antioxidant capacity(FRAP), reducing power를 측정하였다. 총 폴리페놀 함량과 총 플라보노이드 함량을 측정한 결과, 갈참나무로 톱밥재배 한 표고가 3.12 mg GAE/g과 0.84 mg QE/g으로 다른 수종에 비해 가장 높게 나타났다. DPPH 라디칼 소거활성 측정 결과, 10 mg/mL일 때 다른 수종에서 재배한 표고에 비해 갈참나무(89.4%)와 상수리나무(87.9%)에서 가장 높았다. FRAP은 갈참나무일 때 0.425로 가장 높게 나타났고, reducing power도 역시 갈참나무에서 0.359로 가장 높게 나타내었다. 참나무 수종에 따른 표고의 항산화 활성은 갈참나무로 톱밥재배한 것이 가장 우수한 것으로 나타났다. 본 연구의 결과로 동일한 균주를 이용하더라도 톱밥재료에 따라 표고의 항산화 활성이 차이가 있다는 것을 확인할 수 있었다.

• 대한의생명과학회지
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• 제6권4호
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• pp.261-268
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• 2000

할미송이버섯으로부터 혈전용해효소 (FE-2)를 DEAE-cellulose, Mono-S column으로 분리 정제하였다. 이 효소는 분자량이 18.2 kDa 이었으며, ICP-MS (inductively coupled plasma mass spectrometer) 분석 결과 $Zn^{2+}$을 포함하고 있었다. 15번째까지의 N-terminal amino acid 서열은 A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V이고, pH 7.5에서 활성이 가장 큰 염기성 단백질 분해효소로, EDTA와 1,10-phenanthroline에 의해 활성이 감소되는 metalloprotease였다. $Mg^{2+}$, $Zn^{2+}$, Fe$^{2+}$, Co$^{2+}$의 첨가 시 활성이 증가하였으나 Hg$^{2+}$의 경우 활성이 완전히 소멸되었다. 이 효소 (FE-2)는 단백질 분해효소 저해제인 E-64 (trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane), PMSF (phenylmethylsulfonyl fluoride), pepstatin 과 2-mercaptoethanol의 영향을 받지 않으며, 섬유소원 (fibrinogen)과 반응 시 $A\alpha$ chain과 B$\beta$ chain은 분해시키지만 $\gamma$ chain과는 반응하지 않았다.

• 대한수학회논문집
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• 제9권2호
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• pp.393-400
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• 1994

Let M(f,η,ξ,g) be a (2m ＋ 1)-dimensional Sasakian manifold with soldering form dp ∈ ΓHom(Λ/sup q/TM, TM) (dp: canonical vector-valued 1-form) where f,η,ξ and g are the (1,1)-tensor field, the structure 1-form, the structure vector field and the metric tensor of M, respectively.(omitted)

• 한국미생물·생명공학회지
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• 제19권1호
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• pp.76-81
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• 1991

2l 발효조에서 pH6.9, 온도 $32^{\circ}C$일 때 당밀배지를 이용하여 Corynebacterium glutamicum의 영양요구성 유사체 내성변이주에 의한 라이신 발효시 산화환원 전위 (ORP)가 라이신 발효속도의 특성에 미치는 영향을 조사하였다. 희석률이 0.1$h ^1$일때 탄소원이 제한되건 로이신이 제한되건 산소가 제한되지 않는 한 최대의 대당수율 24를 보였으며, 이 때의 산화환원 전윈 값은 -60mV와 -100mV 범위에 해당하였다. 산화화원 전위 값이 -130mV의 매우 낮은 용존산소 조건하에서는 대당수율 밀 $q_s, q_p$ 등의 발효 반응속도 상수값들이 크게 감소하였으며 glvcine, alanine, valine을 포함하는 발효 부산물의 축적량이 매우 높아졌다.

• Molecules and Cells
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• 제21권1호
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• pp.42-51
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• 2006

We investigated the mechanism of contraction induced by S1P in esophageal smooth muscle cells. Western blot analysis demonstrated that $S1P_1$, $S1P_2$, $S1P_3$, and $S1P_5$ receptors existed in the cat esophagus. Only penetration of EDG-5 ($S1P_2$) antibody into permeabilized cells inhibited S1P-induced contraction. Pertussis toxin (PTX) also inhibited contraction, suggesting that it was mediated by $S1P_2$ receptors coupled to a PTXsensitive $G_i$ protein. Specific antibodies to $G_{i2}$, $G_q$ and $G_{\beta}$ inhibited contraction, implying that the S1P-induced contraction depends on PTX-insensitive $G_q$ and $G_{\beta}$ dimers as well as the PTX-sensitive $G_{i2}$. Contraction was not affected by the phospholipase $A_2$ inhibitor DEDA, or the PLD inhibitor ${\rho}$-chloromercuribenzoate, but it was abolished by the PLC inhibitor U73122. Incubation of permeabilized cells with $PLC{\beta}3$ antibody also inhibited contraction. Contraction involved the activation of a PKC pathway since it was affected by GF109203X and chelerythrine. Since $PKC{\varepsilon}$ antibody inhibited contraction, $PKC{\varepsilon}$ may be required. Preincubation of the muscle cells with the MEK inhibitor PD98059 blocked S1P-induced contraction, but the p38 MAP kinase inhibitor SB202190 did not. In addition, co-treatment of cells with GF 109203X and PD98059 did not have a synergistic effect, suggesting that these two kinases are involved in the same signaling pathway. Our data suggest that S1P-induced contraction in esophageal smooth muscle cells is mediated by $S1P_2$ receptors coupled to PTX-sensitive $G_{i2}$ proteins, and PTX-insensitive $G_q$ and $G_{\beta}$ proteins, and that the resulting activation of the $PLC{\beta}3$ and $PKC{\varepsilon}$ pathway leads to activation of a p44/p42 MAPK pathway.

• Geomechanics and Engineering
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• 제35권5호
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• pp.539-554
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• 2023

This article is dedicated to the pursuit of establishing a robust empirical relationship that allows for the estimation of in-situ modulus of deformations (E_m and G_m) within sedimentary rock slope masses through the utilization of Q_slope values. To achieve this significant objective, an expansive and thorough methodology is employed, encompassing a comprehensive field survey, meticulous sample collection, and rigorous laboratory testing. The study sources a total of 26 specimens from five distinct locations within the South Pars (known as Assalouyeh) region, ensuring a representative dataset for robust correlations. The results of this extensive analysis reveal compelling empirical connections between E_m, geomechanical characteristics of the rock mass, and the calculated Q_slope values. Specifically, these relationships are expressed as follows: E_m = 2.859 Q_slope + 4.628 (R² = 0.554), and G_m = 1.856 Q_slope + 3.008 (R² = 0.524). Moreover, the study unravels intriguing insights into the interplay between in-situ deformation moduli and the widely utilized Rock Mass Rating (RMR) computations, leading to the formulation of equations that facilitate predictions: RMR = 18.12 E_m0.460 (R² = 0.798) and RMR = 22.09 G_m0.460 (R² = 0.766). Beyond these correlations, the study delves into the intricate relationship between RMR and Rock Quality Designation (RQD) with Qslope values. The findings elucidate the following relationships: RMR = 34.05e^0.33Qslope (R² = 0.712) and RQD = 31.42e^0.549Qslope (R² = 0.902). Furthermore, leveraging the insights garnered from this comprehensive analysis, the study offers an empirically derived support system tailored to the distinct characteristics of discontinuous rock slopes, grounded firmly within the framework of the Q_slope methodology. This holistic approach contributes significantly to advancing the understanding of sedimentary rock slope stability and provides valuable tools for informed engineering decisions.

• 한국균학회지
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• 제28권1호
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• pp.60-65
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• 2000

할미송이버섯으로부터 3단계를 거처 분자량이 각각 18 kDa(FE-1)과 18.2 kDa(FE-2)인 두 개의 혈전용해효소를 분리 정제하였다. 이 효소들은 지금까지 알려진 혈전용해 효소의 질량에 비해 비교적 작았고 두 효소(FE-1과 FE-2)의 15번째까지의 N-terminal amino acid서열은 A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V로 같았다. FE-1은 ICP-MS의 결과 $Zn^{2+}$을 mole당 0.98 mole 포함하고 있는 metalloprotease로 pH 7.5와 $55^{\circ}C$에서 가장 큰 활성을 나타내고, 1,10-phenanthroline의 첨가 시 저하된 활성은 $Zn^{2+}$나 $Co^{2+}$의 첨가에 의해 재생되었다.

• 동의신경정신과학회지
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• 제19권2호
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• pp.1-14
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• 2008

Objective : This study was designed for find corelation between behavior and physical symptoms in child. Physical symptoms appearing related with weak viscera are selected. And behaviors related to selected symptoms are compared that of children with character type defined by jung, c. G. Method: 497 children ( 3-11 years old ) who have visited two oriental clinic ( one is in Gang-Nam Gu Seoul (282 children), the other is in Joong-Rang Gu Seoul (215 children) ) from Jan. to Jul. 2006 were invested through the questionnaires, which were filled by their parents. These questionnaires have 61 yes-no type questions about behaviors on 12 situations, 36 yes no type questions about physical symptoms in 15 categories. 6 questions expected positive answer in child with weak spleen are categorized sp-q and 6 related weak lung, lu-q, 6 related weak kidney, ki-q. Numbers of positive answers in sp-q, lu-q and ki-q are counted so-index, lu-index and ki-index each. Corelation between behavior questions and 3 symptom indexes are measured and questions shown low significance level ( under 5% in pearson corelation, two-tailed ) are selected. Result: 15 behaviors related sp-index, 12 related lu-index, 12 related ki-index are selected and analysed. Conclusion : 1. Behaviors related sp-index is similar to that of Thinking Type children Grouped by jung, c. G.. 2. Behaviors related lu-index is similar to that of Sensation Type. 3. Behaviors related sp-index is similar to that of Feeling Type.

• 대한임상검사과학회지
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• 제55권3호
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• pp.167-173
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• 2023

본 연구는 한국 성인에서 요산 대 고밀도 지단백 콜레스테롤(uric acid to high density lipoprotein cholesterol, UA/HDL-C) 비율과 요 중 마이크로알부민 대 크레아티닌 비율(uACR)의 연관성을 평가하기 위해 수행되었다. 2019년 국민건강영양조사 자료를 이용하여 20세 이상 성인 5,845명을 대상으로 연구를 수행하였다. 본 연구에서 몇 가지 중요한 발견이 있었다. 첫째, 관련 변수(연령, 성별, 고혈압, 비만, 제2형 당뇨병 및 만성 신장 질환)를 조정한 후, UA/HDL-C ratio의 사분위수가 증가함에 따라 uACR수준이 증가하였다(P=0.002). 둘째, 관련 변수를 조정한 후, UA/HDL-C ratio의 사분위수 1 (Q1)과 비교하여 알부민뇨(uACR≥30.0 mg/g)의 odd ratio (OR)은 Q2(OR, 1.015; 95% confidence interval [CI], 0.757~1.361)와 Q3 (OR, 1.090; 95% CI, 0.811~1.465)에서는 유의하지 않았다. 그러나 Q4 (OR, 1.416; 95% CI, 1.044~1.921)에서 알부민뇨의 발생률은 Q1에 비하여 유의하게 높았다. 한국 성인에서 UA/HDL-C ratio는 알부민뇨와 양의 상관관계가 있었고, UA/HDL-C ratio는 대한민국 성인에서 알부민뇨를 평가하는 유용한 지표로 사용될 수 있을 것으로 사료된다.

• Kyungpook Mathematical Journal
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• 제58권2호
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• pp.347-359
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• 2018

A Kenmotsu manifold $M^n({\phi},\;{\xi},\;{\eta},\;g)$, (n = 2m + 1 > 3) is called a generalized ${\phi}-recurrent$ if its curvature tensor R satisfies $${\phi}^2(({\nabla}_wR)(X,Y)Z)=A(W)R(X,Y)Z+B(W)G(X,Y)Z$$ for all $X,\;Y,\;Z,\;W{\in}{\chi}(M)$, where ${\nabla}$ denotes the operator of covariant differentiation with respect to the metric g, i.e. ${\nabla}$ is the Riemannian connection, A, B are non-vanishing 1-forms and G is given by G(X, Y)Z = g(Y, Z)X - g(X, Z)Y. In particular, if A = 0 = B then the manifold is called a ${\phi}-symmetric$. Now, a Kenmotsu manifold $M^n({\phi},\;{\xi},\;{\eta},\;g)$, (n = 2m + 1 > 3) is said to be generalized ${\phi}-Ricci$ recurrent if it satisfies $${\phi}^2(({\nabla}_wQ)(Y))=A(X)QY+B(X)Y$$ for any vector field $X,\;Y{\in}{\chi}(M)$, where Q is the Ricci operator, i.e., g(QX, Y) = S(X, Y) for all X, Y. In this paper, we study generalized ${\phi}-recurrent$ and generalized ${\phi}-Ricci$ recurrent Kenmotsu manifolds with respect to quarter-symmetric metric connection and obtain a necessary and sufficient condition of a generalized ${\phi}-recurrent$ Kenmotsu manifold with respect to quarter symmetric metric connection to be generalized Ricci recurrent Kenmotsu manifold with respect to quarter symmetric metric connection.