• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.113-117
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• 2006

Heterosis was studied involving two multivoltine silkworm breeds viz, APM1 and SLKSPM through rearing and isozyme analysis. A positive significant heterotic effect was observed in fecundity, hatching % and survivability. The heterobeltiosis was observed only in fecundity and hatching %. Isozyme analysis of ${\alpha}-esterase$ showed variation in loci and allelic expression. The allele with heterozygosity $(Est-2^{12})$ was observed at the Est-2 locus in $F_1$ progeny. Est-3 was observed in $F_1$ progeny, whereas it was completely absent in both parental lines. The present study suggests that the markers ($Est-2^{12}$ and Est-3) targeted for introgression may be useful for the improvement of fecundity and survivability as the phenomenon of heterosis was observed only in $F_1$ progeny.

• 한국균학회지
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• 제37권1호
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• pp.114-116
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• 2009

본 연구는 P. capsici의 난포자를 다량으로 확보하기 위하여 우수한 액체 배지를 선발하고 난포자의 발아율을 조사할 뿐만 아니라 $F_1$ progeny를 확보하기 위하여 수행하였다. 공시한 8개 액체배지 중 carrot broth와 V8C broth가 난포자형성을 위해 가장 적당한 배지였다. 총 11균주의 P. capsici(CapA형)과 P. tropicalis(CapB형)를 선발하였고 P. capsici(CapA형)에 P. tropicalis(CapB형)을 교배하는 서로 다른 9개 조합의 교배에서 얻은 난포자로부터 총 129 균주의 $F_1$ hybrid progeny를 얻었으며 난포자의 발아율은 0.64-4.0%(평균 1.85%)이었다.

• Journal of Plant Biotechnology
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• 제31권2호
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• pp.103-108
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• 2004

본 연구는 나리의 종간잡종 육성체계를 확립하기 위한 기초자료를 얻고자 Oriental과 Asiatic 잡종 (OA-hybrid) 의 후대 획득에 필요한 실험을 수행하여 얻은 결과이다. 체세포 배수화를 통한 이질 4배체 OA-hybrid 3계통으로 화분발아 실험을 수행한 결과, 0-80% 범위로 계통간 차이가 현저하였다. 이질 4배체 OA-hybrid를 자방친 또는 화분친으로 사용하고 Asiatic 또는 Oriental hybrid와 교배시 후대 획득수에 차이가 있었는데 4배체 OA-hybrid를 화분친으로 이용하였을 때, 그리고 상대친을 Asiatic hybrid로 교배하였을 때 후대획득이 현저히 용이하였다. 그러나, 이질 4배체 F$_1$ OA-hybrid를 자방친으로 사용하거나 Oriental hybrid와 교배하면 후대 획득률이 현저히 저하되거나 또는 전혀 후대생산이 이루어지지 않았다. 이질 4배체 F$_1$ OA-hybrid와 교배시 상대친의 ploidy level은 4배체 보다는 2배체가 유리하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.1-4
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• 1999

Beijing White Chickens laying larger eggs and smaller eggs were respectively used as parental individuals for mating to produce the F1 progeny and then the F1 progeny individuals mated to produce 125 individuals of the F2 progeny. Three bands associated with the egg weight performance were identified from DNA fingerprints of the 125 individuals generated with a bovine minisatellite probe BM6.5B. The simple linear correlation analysis showed that the coefficients of correlation between frequencies of the three bands (DB1, DB2 and DB3) and egg weights were -0.6, -0.6 and 0.9, respectively.

• 한국가축번식학회지
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• 제25권1호
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• pp.85-90
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• 2001

본 연구는 growth hormone receptor(GHR) gene의 동물생리에 미치는 영향을 연구하기 위해 metallothionein promoter와 GHR gene을 이용하여 생쥐의 1-cell 수정란에 DNA 미세주입법에 의해 형질전환생쥐를 생산하였다. 세마리의 형질전환생쥐가 생산되었는데 DNA 분석결과 4~8 copy의 GHR 유전자를 지닌 것으로 확인되었다 이들 세 마리의 GHR 형질전환생쥐를 정상 형질전환생쥐와 교미시켜 F$_1$과 F$_2$ 새끼를 생산하였는데 이들의 전달율은 F$_1$에서 20~50%였고 F$_2$에서는 약 50%를 나타내어 모자익형태로 유전자가 정착되었음을 확인할 수 있었다. 3주령까지의 사망률은 Fl과 F2 새끼에서 약 10~30%를 나타내어 GHR유전자의 발현이형질전환생쥐의 초기 사망에 영향을 미치는 것으로 나타났다.

• Mycobiology
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• 제40권2호
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• pp.100-106
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• 2012

Interest in commercial cultivation and product development of Cordyceps species has shown a recent increase. Due to its biochemical and pharmacological effects, Cordyceps militaris, commonly known as orange caterpillar fungus, is being investigated with great interest. Cultivation of C. militaris has been practiced on a large scale in order to fulfill a demand for scientific investigation and product development. Isolates of C. militaris can be easily established from both spores and tissue. For isolation of spores, ascospores released from mature stromata are trapped in sterile medium. Multi-ascospore isolates, as well as combinations of single ascospore strains, are used for production of fruiting bodies. Progeny ascospore strains can be isolated from artificial fruiting bodies, thus, the cycle of fruiting body production can be continued for a long period of time. In this study, we examined fruiting body production from multi-ascospore isolates and their progeny strains for three generations. $F_1$ progeny strains generally produced a larger number of fruiting bodies, compared with their mother multi-ascospore isolates; however, $F_2$ and $F_3$ progeny strains produced fewer fruiting bodies. Optimum preservation conditions could help to increase the vitality of the progeny strains. In order to retain the fruiting ability of the strains, further testing of various methods of preservation and different methods for isolation should be performed.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.729-732
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• 2000

Weight records from birth to calving and calving scores of 407 two-year old heifers and weights of their offspring from birth to one year of age were used to study the effects of sire birth weight on maternal traits of their female progeny. The heifers (G1) were the progeny of 81 sires (G0) and were classified into three classes based on their sires' birth weights (High, Medium and Low). The heifers were from three distinct breed-groups and were mated to bulls with medium birth weights within each breed-group to produce the second generation (G2). The data were analyzed using a covariance model. The female progeny of high birth-weight sires were heavier from birth to calving than those sired by medium and low birth-weight bulls. The effect of sire birth weight on calving difficulty scores of their female progeny was not significant. Grand progeny (G2) of low birth-weight sires were lighter at birth than those from high birth-weight sires (p<0.05) but they did not differ significantly in weaning and yearling weights with the other two Grand progeny groups. The results indicated that using low birth weight sires would not result in an increase in the incidence of dystocia among their female progeny calving at two-year of age and would not have an adverse effect on weaning and yearling weights of their grand progeny.

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.5-8
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• 1999

Weight records from birth to calving and calving scores of 407 two-year old heifers and weights of their offspring from birth to one year of age were used to study the effects of sire birth weight on maternal traits of their female progeny. The heifers ($G_1$) were Ihe progeny of 81 sires ($G_0$) and were classified into three classes based on their sires' birth weights (High, Medium and Low). The heifers were from three distinct breed-groups and were mated to bulls with medium birth weights within each breed-group to produce the second generation ($G_2$). The data were analyzed using a covariance model. The female progeny of high birth-weight sires were heavier from birth to calving than those sired by medium and low birth-weight bulls. The effect of sire birth weight on calving difficulty scores of their female progeny was not significant. Grand progeny (G2) of low birth-weight sires were lighter at birth than those from high birth-weight sires (p < 0.05) but they did not differ significantly in weaning and yearling weights from the other two Grand progeny groups. The results indicated that using low birth weight sires would not result in an increase in the incidence of dystocia among their female progeny calving at two-year of age and would not have an adverse effect On weaning and yearling weights of their grand progeny.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권1호
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• pp.49-55
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• 2005

Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.

• 한국연초학회지
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• 제22권1호
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• pp.71-75
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• 2000

The genetic makeup could be the most important among many factors affecting yield and quality of tobacco(Nicotiana tabacum L.). The mammoth gene found in N. tabacum is associated with greater leaf number and poor leaf quality. This study was carried out to obtain the basic information about the inheritance of mammoth gene and white flower color. Two flue-cured breeding lines, KF 9373-2 and KF 8832-85, F$_1$, F$_2$, two parents backcrossed with F$_1$, and F$_3$ lines derived from cross of above two lines were investigated for flowering type(mammoth gene) and flower color. All plants of F$_1$ population revealed normal flowering type and pink flower color. The progeny of F$_2$ generation was segregated into the phenotypic ratio of 9 : 3 : 3 : 1 with normal flowering type and pink flower color, normal and white, non flowering type(NF) and pink, and NF and white, respectively. Among the progenies of back-crossing populations, the flowering type showed a segregation ratio of 1 : 1 as normal and NF in BP$_1$ and flower color did also 1 : 1 as pink and white in BP$_2$. All lines have the mammoth gene in F$_3$. that were selected in F$_2$ progeny as non flowering. But 9 lines among 14 were segregated with 3 : 1 as pink and white flower in F$_3$. which were selected in F$_2$ as pink flower color. These results indicated that the characters of mammoth gene and white flower were controlled by a pair of recessive genes, respectively.