• 한국식품위생안전성학회지
• /
• 제26권1호
• /
• pp.12-15
• /
• 2011

코엔자임 Q10은 우리나라뿐 아니라 여러나라에서 각광받고 있는 건강기능식품 중 하나로 이에 대한 분석법을 마련하고 이에 대한 분석법 검증을 하고자하였다. 분석기기로는 HPLC를 사용하였으며 분석법 검증을 위하여 직선성, 선택성, LOD, LOQ, 정밀성, 정확성을 확인하였다. 또한 현재 유통 중인 개별인정형 건강기능식품의 코엔자임 Q10 함량을 모니터링 한 결과 모든 제품이 적합함을 알 수 있었다. 따라서 확립된 분석 방법은 신속하고 효과적으로 분석하는데 이용될 수 있을 것이며, 개별인정형제품의 코엔자임 Q10이 공전에 등재시 본 연구결과가 시험법으로 활용할 수 있을 것으로 사료된다.

• Journal of the Korean Data and Information Science Society
• /
• 제19권4호
• /
• pp.1091-1100
• /
• 2008

The purpose of this study is to find out how energy substrate's utilizations in the maximal exercise-cardiorespiratory function and the energy metabolic variation- have the effect on before and after the supplements of coenzyme Q10(100mg per day for 8 weeks) based on the male students(CoQ10=8, placebo=8) of high school. The results of this study are as follows: Maximal oxygen uptake and maximal oxygen per weight are found to have the significant increase respectively in supplement group. Glucose is found to have the significant decrease after the supplement of CoQ10. And then FFA is found to have the significant increase. In the above results, the supplements of CoQ10 and cardiorespiratory function in the maximal exercise are found to have the improvement. CoQ10 in the anaerobic exercise spares the energy's utilization of Glucose and then is found to have the increase of the utilization of FFA. CoQ10 in the aerobic exercise is reported to have the increase of the utilization of FFA and then CoQ10 in the maximal exercise is also recognized to have the increase of the utilization of FFA, or energy substrates.

• Nutrition Research and Practice
• /
• 제6권5호
• /
• pp.414-420
• /
• 2012

Forty guinea pigs were divided into four groups and fed 0.04% cholesterol based control diet, plus 0.05% simvastatin, and statin plus 0.1% CoQ10 or 10% Ardisia Japonica Blume (AJB) leave powder for 4 weeks. Plasma total cholesterol levels decreased significantly in all groups fed the statin-containing diet compared with that in guinea pigs fed the control diet (P < 0.01). Plasma and liver triglycerides decreased significantly in the statin plus CoQ10 group compared with those in the control (both P < 0.05). Maximum platelet aggregation was significantly higher in the statin plus CoQ10 group than that in the other groups (P < 0.05). Na-K ATPase activity increased in the statin group and decreased in the statin plus CoQ10 group (P < 0.01). Na-K co-transport and Na passive transport decreased significantly in the control group compared with those in the other groups (both P < 0.05). Intracellular Na was highest in the statin group and lowest in the statin plus CoQ10 group and was correlated with Na-K ATPase activity. Thiobarbituric acid reactive substance production in platelet-rich plasma and liver tended to decrease in the statin plus CoQ10 group compared with those in the other groups. Plasma glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase increased significantly in the statin group compared with those in the control (P < 0.05). These result suggest that antioxidant rich AJB did not have positive effects on cardiovascular disease parameters. The statin plus CoQ10 seemed to decrease cholesterol more efficiently than that of statin alone.

• 운동영양학회지
• /
• 제13권1호
• /
• pp.29-35
• /
• 2009

The purpose of this study was to investigate the effects of oral supplementation of coenzyme Q10 (CoQ10) for 4 weeks on the plasma free oxygen radical and total antioxidant capacity at resting and after one bout exercise in healthy old men. Thirty volunteers with an average (+/-SD) age of 62.59+/-5.3 years participated in this study and were divided with three groups; CoQ10 (200 mg daily) group, vitamin C & E (800mg, 400 IU daily) group, and placebo group. A cycle exercise (60% HRR) test was performed at the end of study. Blood samples were taken for the analyses at rest and pre-, post-, 30min after cycle exercise, before and after the 4 weeks of supplementation. After supplementation, there were no significant differences in the plasma free oxygen radical levels and total antioxidant capacity at resting. Plasma free oxygen radical level and total antioxidant capacity in three groups were significantly elevated after exercise, however, it did not vary significantly between groups. CoQ10 supplementation showed significant difference in total antioxidant capacity during recovery phase compared with placebo group. Our results demonstrated that supplementation of CoQ10 in healthy old men improve blood total antioxidant capacity after one bout exercise, despite no alteration of plasma free oxygen radical levels.

• Journal of Microbiology and Biotechnology
• /
• 제21권5호
• /
• pp.494-502
• /
• 2011

Coenzyme $Q_{10}$ ($CoQ_{10}$) is a widely used supplement in heart diseases treatment or antioxidative dietary. The microbial production of $CoQ_{10}$ was enhanced by addition of solanesol and novel precursors recovered from waste tobacco. The novel precursors were separated by silica gel and identified as ${\alpha}$-linolenic acid (LNA) and butylated hydroxytoluene (BHT) based on the effect on $CoQ_{10}$ production and GC-MS. The effects of novel precursors on $CoQ_{10}$ production by Sphingomonas sp. ZUTE03 were further evaluated in a two-phase conversion system. The precursor's combination of solanesol (70 mg/l) with BHT (30 mg/l) showed the best effect on the improvement of $CoQ_{10}$ yield. A maximal $CoQ_{10}$ productivity (9.5 mg $l^{-1}$ $h^{-1}$) was achieved after 8 h conversion, with a molar conversion rate of 92.6% and 92.4% on BHT and solanesol, respectively. The novel precursors, BHT and LNA in crude extracts from waste tobacco leaves, might become potential candidates for application in the industrial production of $CoQ_{10}$ by microbes.

• Clinical Nutrition Research
• /
• 제12권4호
• /
• pp.257-268
• /
• 2023

Migraine is a common neurological disease correlated with oxidative stress and lipid profile disorders. The present study was designed to determine the effects of Coenzyme Q10 (Co-Q10) supplementation on oxidative status and lipid profile in migraine individuals. This clinical trial was conducted on 84 females aged 18-50 years, diagnosed for episodic migraine according to the International Headache Society. Subjects were randomized to receive either Co-Q10 supplement (400 mg/day) or placebo for 12 weeks. Lipid profile and oxidative stress indices including malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured before and after intervention in both groups. Also, anthropometric indices, dietary intakes, and clinical features were collected. Data analysis was conducted using SPSS version 16. Seventy-seven of the participants, with mean age of 33.70 ± 7.75 years, completed the study. After 12-week intervention, Co-Q10 led to a significant decrease in MDA levels compared to placebo (p = 0.009), with no effect on TAC levels (p = 0.106). A significant increase in serum Co-Q10 concentration and high-density lipoprotein cholesterol (HDL-C) level in Co-Q10 group was observed, but no significant differences were found in other lipid profile variables (low-density lipoprotein cholesterol, triglycerides and total cholesterol). Among anthropometric variables, Co-Q10 only caused a significant reduction in body fat percentage (BFP), but we did not find any significant changes in others. A 12-week Co-Q10 supplementation led to significant improvement in clinical features, BFP, and HDL-C level among migraine individuals.

• Journal of Microbiology and Biotechnology
• /
• 제17권6호
• /
• pp.1045-1048
• /
• 2007

Two genes, dps encoding decaprenyl diphosphate synthase and dxs encoding 1-deoxy-D-xylulose 5-phosphate synthase, were isolated from Rhizobium radiobacter ATCC 4718. DNA sequencing analysis of the dps and dxs genes revealed an open reading frame of 1,077 bp and 1,920 bp, respectively. The heterologous expression in Escherichia coli BL21(DE3) was carried out in order to identify their functions. Recombinant E. coli BL21(DE3) harboring the dps gene produced $CoQ_{10}$ as well as $CoQ_8$ and $CoQ_9$, whereas E. coli harboring only the dxs gene produced more $CoQ_8$ compared with the wild-type E. coli. Additionally, the coexpression of dps and dxs genes in E. coli was carried out. The recombinant E. coli harboring only the dps gene produced $0.21{\pm}0.04\;mg/l$ of $CoQ_{10}$, whereas the coexpressed E. coli with dps and dxs genes produced $0.37{\pm}0.07\;mg/l$ of $CoQ_{10}$. HPLC analysis also showed that the $CoQ_{10}$ fraction (100% of the total CoQs distribution) was increased from $15.86{\pm}0.66%$ (only dps) to $29.78{\pm}1.80%$ (dps and dxs).

• Physical Therapy Rehabilitation Science
• /
• 제1권1호
• /
• pp.6-12
• /
• 2012

Objective: Coenzyme (CoQ10) is an enzymatic co factor used in normal cellular metabolism. Recent evidence shows that in people with heart disease it can reverse endothelial cell damage in the blood vessels. It is also a potent antioxidant. Design: One group pretest-posttest design. Methods: In the present study, endothelial function was evaluated using the response to occlusion and heat before and 2 weeks after administration of CoQ10, 300 mg/day. Thirty Eight subjects, who are physical therapy students, participated in a series of experiments to see if taking 300 mg of CoQ10 daily for 2 weeks would impact resting blood flow in the forearm skin and the blood flow response to 4 minutes of vascular occlusion and the response to local heat ($42^{\circ}C$) for 6 minutes. Results: The results showed that, for this population, there was no difference in the response to heat. However, the response to occlusion was improved after administration of CoQ10. Conclusions: It would appear that in a young population CoQ10 has no effect on the nitric oxide vasodilator pathway in skin but does influence other vasodilator pathways.

• 한국축산식품학회지
• /
• 제38권6호
• /
• pp.1294-1304
• /
• 2018

The aims of this research were to examine the effect of heating temperature (65, 75, and $85^{\circ}C$) and $CaCl_2$ concentration level (3, 4, and 5 mM) on the physicochemical properties of ${\beta}$-lactoglobulin (${\beta}$-lg) nanoemulsions (NEs) and to study how the droplet size of NEs affects the bioaccessibility (BA) of coenzyme $Q_{10}$ ($CoQ_{10}$). The droplet size of NEs and BA of $CoQ_{10}$ was assessed by particle size analyzer and UV-Vis spectrophotometer, respectively. An increase in heating temperature and $CaCl_2$ concentration level resulted in a significant (p<0.05) increase in the droplet size of NEs while there were no significant differences in polydispersity index and zeta-potential of NEs. When NEs containing $CoQ_{10}$ were incubated in simulated small intestinal phases, an increase in the droplet size and polydispersity index of NEs was observed. This indicated that NEs were not stable in small intestine and digestion of NEs occurred. As heating temperature and $CaCl_2$ concentration level were decreased, a significant (p<0.05) increase in BA of $CoQ_{10}$ was observed. There was a significant (p<0.05) increase in BA of $CoQ_{10}$ with a decrease in the droplet size of NEs. In conclusion, heating temperature and $CaCl_2$ concentration level were key-parameters affecting the initial droplet size of NEs and BA of $CoQ_{10}$ was negatively correlated with initial droplet size of NEs.

• Journal of Ecology and Environment
• /
• 제35권3호
• /
• pp.203-212
• /
• 2012

The physiological effects of elevated $CO_2$ concentration and temperature were examined for Quercus gilva and Q. glauca grown under control (ambient $CO_2$ and temperature) and treatment (elevated $CO_2$ and temperature) conditions for 39 months. The objective of the study was to measure the long-term responses, in physiological parameters, of two oaks species exposed to elevated $CO_2$ and temperature. The photosynthetic rate of Q. gilva was found to be decreased, but that of Q. glauca was not significantly affected, after long-term exposure to elevated $CO_2$ and temperature. Stomatal conductance of Q. glauca was reduced by 21.7%, but that of Q. gilva was not significantly affected, by long-term exposure to $CO_2$ and temperature. However, the transpiration rate of the two oak species decreased. Water use efficiency of Q. gilva was not significantly affected by elevated $CO_2$ and temperature, while that of Q. glauca was increased by 56.6%. The leaves of Q. gilva grown under treatment conditions had an increased C:N ratio due to their reduced nitrogen content, while those of Q. glauca were not significantly affected by long-term exposure to elevated $CO_2$ and temperature. These results suggest that the long-term responses to elevated $CO_2$ and temperature between Q. gilva and Q. glauca are different, and that Q. gilva, the endangered species, is more sensitive to elevated $CO_2$ and temperature than Q. glauca.