• 한국식품영양학회지
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• 제22권1호
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• pp.14-19
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• 2009

본 연구는 산업적으로 활용할 수 있는 lipase를 개발하기 위해 갯벌에서 분리된 Gelidibacter sp. YH333와 Vibrio sp. YH339에 의해 생산되는 lipase의 특성에 대해 연구하였다. 분리 균주들로부터 세포 외로 방출하는 lipase의 양은 세포수의 증가와 비례하여 급격하게 증가하였다. 분리 균주들에 의해 생산되는 lipase는 대부분 세포 외로 lipase를 방출함으로 lipase가 세포 밖으로 constitutively하게 분비됨을 알 수 있었다. 두 균주에서 생산된 lipase 모두 p-nitrophenyl laulate(C12:0)에서 가장 높은 활성을 보여 주었다. Gelidibacter sp. YH333에서 생산된 lipase가 Vibrio sp. YH339에 의해 생성된 lipase보다 모든 기질에 있어 높은 활성을 보여주고 있다. Gelidibacter sp. YH333에서는 약 50 KDa, 25 KDa 등 두 개의 lipase가 확인되었고 Vibrio sp. YH339에서는 약 50 KDa에 해당하는 lipase가 확인되었다.

• 한국축산식품학회지
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• 제34권2호
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• pp.185-191
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• 2014

This study was performed to determine the meat quality characteristics of pork loin and belly from 3 different three-way crossbred pigs: Yorkshire ${\times}$ Landrace ${\times}$ Duroc (YLD), Yorkshire ${\times}$ Chester White ${\times}$ Yorkshire (YCY), and Yorkshire ${\times}$ Berkshire ${\times}$ Duroc (YBD). Each of the twenty crossed pigs were randomly selected with their live weights at the range of 110-120 kg. After being slaughtered and cooled at $0^{\circ}C$ for 24 h in a chilling room, the parts of loin and belly on the left side of the cooled carcasses were cut and prepared for analysis. The intramuscular fat contents of the loins from YLD were higher than those of the other crossbreds (p<0.05), and the YCY bellies had the highest moisture contents (p<0.05). Water holding capacity (WHC) of the loins from YCY were higher than the other crossbreds (p<0.05). Shear force values of the YBD loins were higher than the others (p<0.05). The TBARS values of YCY loins were significantly lower than the others at 0 d, but the difference disappeared after 14 d of storage. Sensory scores of YLD were ranked higher than the YCY or YBD in both the loins and bellies (p<0.05). The relatively high sensory values of YLD crossbred pork could be explained by the better WHC, the low shear forces, and the higher fat contents. The results indicated that the meat qualities could be altered by three-way crossbreeding.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.550-556
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• 2009

This study was designed to evaluate the effects of dietary prebiotic (mannan oligosaccharide = MOS) and probiotic (Saccharomyces cerevisiae = SC) in finishing turkey diets on carcass, cut yield, meat composition and colour. A total of 72 ten-weeksold Big6 male turkey poults were used in the trial. There were eight replicate floor pens per floor with three birds in each. The experiment lasted up to 20 wks of age. The trial was set up as a completely randomized design with 3 dietary treatments. The treatments were: i) negative control (C, no additive); ii) MOS 1 g per kg of diet and iii) SC 1 g per kg of diet (strain SC47, $300{\times}10^{10}$ CFU/kg). Body weight (BW) and feed intake were determined for each of the two week intervals. Twenty-four birds were slaughtered and eviscerated to determine carcass, carcass parts and internal organ weights at 20 wks of age. Meat colour and pH levels were measured 24 h after slaughter. The dietary treatments did not affect BW and average daily gain during the trial (p>0.05). The average daily feed intake and feed conversion ratio of turkey toms fed with MOS were higher than those of control and SC groups during the overall period (p<0.05). The dietary treatments did not affect carcass yield, breast meat, thigh, wing, liver, heart, empty gizzard, intestine, and abdominal fat pad proportions and meat pH, composition and pigmentation (p>0.05). These results suggest that the addition of MOS and SC is not likely to produce any performance or carcass characteristics in finishing turkeys at 10 to 20 wks of age.

• 약학회지
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• 제50권4호
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• pp.220-227
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• 2006

The therapeutic effect of tissue cultured root of mountain ginseng (Panax ginseng) (tcMG) on 2,3,7,8-tetrachlorodaibenzo-p-dioxin (TCDD) induced toxicity in rat was investigated. The rats were assigned into three groups (10 rats/group), control, TCDD exposed group and tcMG treated group after TCDD exposed. $50\;{\mu}g/kg$ of TCDD was injected by i.p. for TCDD exposed group and 30 mg/kg of tcMG saponin was administered for 4 weeks by oral gavage for tcMG treated group. The weights of body, spleen, kidney, thymus, testes and epididymides were decreased in the single TCDD treatment. However these organs was significantly recovered by tcMG saponin except thymus (p<0.05). tcMG decreased the level of hepatic demage maker enzymes, AST and ALP. It also lowered total cholesterol and triglyceride. The level of serum triglyceride was significantly decreased in tcMG saponin treated group compared with the control. Histopathological examination revealed morphological change in the liver spleen, thymus and testes of TCDD treated rats. However they were relatively well preserved in the tcMG treatment group. In conclusion, TCDD induced toxicity was some repaired by tcMG. tcMG may be useful for prevention and treatment of TCDD induced toxicity.

• Neonatal Medicine
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• 제15권2호
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• pp.151-159
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• 2008

목 적 : 최근 신생아 집중 치료술의 발달로 미숙아의 생존율이 증가하면서 이로 인한 미숙아의 합병증이 중요한 문제가 되고 있다. 백혈병양반응 (leukemoid reaction)은 골수 이외의 원인에 의해 절대호중구수 30,000/$mm^3$ 이상인 경우로 이에 대한 산전 위험 요소와 출생후 합병증과 예후에 관한 상관관계에 대해 잘 알려져 있지 않아 치료와 대책이 없는 상황이다. 이에 신생아 집중치료실에 입원한 미숙아를 대상으로 혈중 백혈구수를 분석하고 합병증과 예후를 대조군과 비교 분석하였다. 방 법 : 2005년 6월부터 2007년 7월까지 동국대학교 일산병원 신생아 집중 치료실에 입원한 재태 기간 37주미만의 미숙아 중 2,500 g 미만의 적정 체중아 252명을 후향적으로 의무기록을 통하여 조사하였다. 총혈구검사를 출생 직후 및 매일 또는 매주 시행하였고 절대호중구수가 30,000/$mm^3$ 이상인 경우를 백혈병양반응으로 정의하였다. 산전 위험 요소는 산모의 나이, 조기 양막 파수,융모양막염, C-반응 단백질 수치를 조사하였고 미숙아들의 합병증 및 예후는 재태 기간, 출생 체중, Apgar 점수, 신생아 호흡 곤란 증후군, 인공호흡기 사용일수, 기관지폐 형성 이상, 동맥관 개존, 뇌실 내 출혈, 괴사 장염, 미숙아 망막증, C-반응 단백질 수치, 산전 스테로이드 사용, 사망률을 후향적으로 조사하여 환자군과 대조군을 비교 분석하였다. 결 과 : 백혈병양반응을 보인 환자군은 총 8명(3.14%)이었다. 산전 관련인자로 산모의 연령과 조기 양막 파수와는 관련이 적었으나 융모양막염이 있는 경우(P=0.010)와 분만 전 산모의 높은 C-반응 단백질 수치(P<0.001)는 유의한 관련이 있었다. 환자군과 대조군을 통계적으로 비교하여 볼 때 환자군의 재태간(P=0.005)이 짧고 출생 체중(P=0.011)이 작았으며 1분 Apgar 점수(P=0.045)와 5분 Apgar 점수(P=0.014)가 낮았다. 환자군이 신생아 호흡 곤란 증후군(P=0.004)과 기관지폐 형성 이상(P< 0.001)과 관련이 컸으며 인공호흡기를 사용한 일수가 길었다(P<0.001). 체중이 비슷한 1,000 g 미만의 초극소 저체중 출생아에서 임상적 특징 및 합병증의 비교에서는 인공호흡기 사용일수(P=0.041)와 기관지폐 형성 이상의 발생률(P=0.006)이 백혈병양반응과 유의한 연관을 보였다. 환자군에서 출생 직후의 C-반응 단백질 수치가 유의하게 높았다(P<0.001). 환자군과 대조군의 특징과 합병증을 다변량분석을 실시하였을 때 기관지폐 형성 이상과 환자군의 C-반응 단백질 수치가 비교위험도가 각각 60.12, 4.57로 95%신뢰구간에서 유의한 값을 보였다. 전체 대상 환자군에서 동맥관 개존과 신생아 괴사 장염의 발생 비율이 높았으나 통계적으로 유의하지 않았다. 뇌실 내 출혈, 미숙아 망막증, 사망률, 산전 스테로이드 사용은 환자군과 대조군에서 차이는 없었다. 결 론 : 백혈병양반응은 산전 감염과 관련이 있고 이로 인해 백혈구수가 증가하여 과도한 염증반응이 출생후 기관지에 영향을 미쳐 기관지폐 형성 이상을 유발하는 것으로 생각된다. 출생 전 산모의 감염에 대한 산과적 치료와 출생 후 미숙아의 호흡기의 치료에 대한 연구가 필요할 것으로 생각된다.

• 한국잠사곤충학회지
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• 제45권1호
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• pp.46-57
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• 2003

정련 액에서 수용성 세리신을 얻고, 남은 난용성 세리신을 여러가지 효소로 가수분해하여, 효소 가수분해 특성을 알아보고. 아미노산 조성과 분자량을 조사하여 다음과 같은 결론을 얻었다. 1. 효소 가수분해 시 kojizyme과 flavourzyme의 최적조건은 pH 7, 온도 5$0^{\circ}C$이었고, protamex와 alcalase는 처리 pH의 증가에 따라 활성이 높았고 최적온도는 6$0^{\circ}C$이었다. 2 효소 가수분해 시 용해도 측정에서는 효소 처리농도 증가에 따라 용해도도 높았으며, 4시간 이상의 처리에서는 아주 소폭으로 용해도가 증가하였다. 3. 효소 가수분해 시 흡광도를 이용한 용해도 측정에서는 효소 처리농도 증가에 따라 흡광도도 높았으며. 4시간 이상의 처리에서도 계속하여 흡광도가 증가하였다. 4. 효소 가수분해 시 말단기 정량에 의한 평균중합도 측정에서는 처리시간과 농도의 증가에 따라 중합도가 감소하였다. 5. 아미노산 분석 결과 정련 후 채취한 low와 high의 아미노산 조성이 비슷하고, PK와 PP및 PA의 아미노산 조성이 비슷하였으며, low와 high는 PK, PP, PA보다 세린과 타이로신의 함량이 많았고, 프롤린은 검출되지 않았다. 6. GPC에 의한 분자량 측정결과 중량평균분자량(Mw) 크기는 PK＞high＞PP＞low＞PA 순서이었으며, PK와 PA의 Mw는 각각 9,800, 905이었다

• 한국가금학회:학술대회논문집
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• 한국가금학회 2000년도 춘계 산학협동 심포지움 Proceedings
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• pp.23-36
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• 2000

Hurdle technique was used to remove cholesterol efficiently from liquid egg yolk. The quality of the low cholesterol egg products from the process were evaluated. From the 75 % cholesterol reduced egg yolk through $\beta$-cyclodextrin treatment. 2 times weight of soy bean oil was added to the egg yolk and homogenized followed by centrifuged to be maximized to remove cholesterol. When the pH of the yolk was adjusted to 9, 92 % of cholesterol was removed while 95.4 % of cholesterol was removed when 3 times weight of soy bean oil was added to the egg yolk. As the results of application of supercritical carbon dioxide extraction to the 75 % cholesterol reduced egg yolk through ${\beta}$-cyclodextrin treatment, 92.5 % of the cholesterol was removed from the egg yolk at $35^{\circ}C$, 4,500 psi, for 4 hours under co-solvent. The quality characteristics of the produced low cholesterol egg products were analysed. The cholesterol reduced egg yolk produced from ${\beta}$-cyclodextrin and soy bean oil treatment showed the lower emulsion capacity compared with control. The fatty acid composition of the cholesterol reduced egg yolk produced from ${\bet}a$-cyclodextrin and soy bean oil treatment showed increased C18:2 and C18:3 compared with control while decreased C16:1 and C18: 1 compared with control. The saponification method with extracting solvent of hexane showed that cholesterol concentration was 28.1 %. The quantity of hydrolysis solution(95 % ethanol : 33 % KOH = 94 : 6) was varied from 40 to 80 times of sample weights and the cholesterol concentration of 35.7 % was the highest result in the 60 times(v/w) hydrolysis solution. Cholesterol concentration of 35.7 % was recovered at the first trial with saponification method. but it could be improved up to 95.7 % after 4 times repetitive purification.

• 한국수산과학회지
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• 제40권4호
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• pp.187-192
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• 2007

To prepare oligouronic acids from high-molecular-weight alginates prepared from sea tangle (Laminaria japonica), the alginates were hydrolyzed at various temperatures for 1 hr at pH 5.0. The effects of hydrolysis temperature $(H_{Temp})$ on the average molecular weight (AMW) and distribution ratio of MW (DR) in the hydrolyzed alginates were investigated. As $(H_{Temp})$ increased, the AMW of the alginates decreased exponentially; in addition the DR of the alginates with MW>500 kDa decreased exponentially, while the DR of those with MW=50-100 and MW<50 kDa increased exponentially. For the alginates with MW=300-500 and MW=100-300 kDa the DR increased exponentially until $H_{Temp}$ reached $80^{\circ}C$, and then decreased exponentially at above $80^{\circ}C$. AS $H_{Temp}$ increased, the MW cutoff size and AMW Of the alginates fraction With the highest DR both decreased. For $H_{Temp}<60^{\circ}C$, the MW cutoff size with the highest DR was MW>500 kDa; the DR was 39-67% and the AMW 1,000-1,300 kDa. For $H_{Temp}$ $80^{\circ}C$, the MW cutoff size with the highest DR was MW=300-500 kDa and the DR was about 33% and the AMW about 400 kDa. For $H_{Temp}\;100-121^{\circ}C$, the MW cutoff size with the highest DRs was MW=50-100 kDa, with a DR of 39-44% and an AMW of 70-80 kDa.

• 한국환경과학회지
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• 제33권1호
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• pp.17-25
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• 2024

This study aimed to determine the most suitable coir substrate mixing ratio for optimizing the growth and yield of the "lpduelkkae 1" cultivar. We comprehensively analyzed the physicochemical properties, growth, and yields of four different substrate combinations: perlite (coir with mixing ratios of 70:30 (PC30), 50:50 (PC50), and 30:70 (PC70)) and 100% coir (C100). The results revealed substantial differences in substrate properties. C100 exhibited the highest total porosity and the lowest solid phase, indicating excellent air permeability. The pH levels and electrical conductivity (EC) values ranged from 5.4-6.8 and 1.2-3.1 dS·m^-1, respectively. Leaf growth parameters, including length, width, and dry weight, showed positive correlations with high coir ratios, except for PC30. PC70 and C100 outperformed other substrates in stem growth, exhibiting superior stem diameter and fresh and dry weights. The quantity of marketable leaves was the highest in the C100 substrate. Furthermore, C100 comprised integrated levels of essential nutrients, such as Ca and Mg, owing to its high coir content. In conclusion, a coir ratio of approximately 70% (v/v) should be maintained in the substrate for creating an optimal cultivation environment. Furthermore, the selection of humidity-resistant varieties as well as precise nutrient and moisture management for different seasons and growth stages are crucial for a successful perilla leaf hydroponic cultivation.

• 한국작물학회지
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• 제61권2호
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• pp.131-137
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• 2016

This study was conducted to investigate the effects of temperature and shade, which are basic environmental conditions, on growth, yield, inorganic components, and general components of Codonopsis lanceolata, in order to obtain basic data for improving yield capacity. In natural light, in the 15, 20, and $25^{\circ}C$ groups, the plant heights ranged between 218.9 cm and 223.9 cm, and there was no significant difference between groups. However, the leaf size was larger in shade, and the leaf area was significantly larger in the 15 and $30^{\circ}C$ groups. In natural light, root length and diameter were shorter and thinner when the temperature was higher, and growth was highly suppressed at $30^{\circ}C$. With regards to macroelements, the contents of Na, Mg, and P increased as temperature increased, regardless of the plant part; however, no constant tendency was observed in K and Ca according to temperature. The contents of Mg and Ca (from highest to lowest) were in the order leaf>stem>root, whereas the contents of Na, P, and K were in the order stem>leaf>root. Contents of general components varied according to temperature, and were highest at $30^{\circ}C$. While the plant height was increased under the constant $25^{\circ}C$+DIF (Difference between day and night temperature) condition, growth was suppressed in the -DIF group, in which the night temperature was higher than the day temperature, which suggests that a change in night temperature is one of the factors that affects the growth of C. lanceolata. As in the growth of the above-ground parts, fresh weight of the root was high in the constant $25^{\circ}C$ group and +DIF group. Notably, it was more than 2.5 times the fresh weights in the constant $15^{\circ}C$ group, constant $20^{\circ}C$ group, and -15 DIF group.