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Studies on Kokja of High Quality(Part 1) Preparation of new type Kokja and its activity (국자(麴子)의 개량(改良)에 관(關)한 연구(硏究)(제(第) 1 보(報)) 개량국자(改良麴子)의 제조(製造) 및 그 능력(能力))

  • Chung, Ho-Kwon
    • Korean Journal of Food Science and Technology
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    • v.2 no.1
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    • pp.88-92
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    • 1970
  • 1. Aflatoxin free and highly active new type kokja was made with strong two strains of yeast (Y-16, Y-21) and two strains of highly saccharifying active Aspergillus (A-32, A-11). And they were isolated from many kinds of kokja (over hundred) collected from all over Korea. 2. Activities of new type kokja was compared with old type kokja, and results were as following. (i) Saccharifying activity; New type kokja B, C and D were excellent (over 8. 0%), and home made old type kokja were poor quality. (ii) Alcohol fermentation; New type kokja C and D were over 12% and the old type K-12 and K-161 were low activity (less than 10%). (iii) Acid fermentation; Old type kokja K-2o and K-156 (industrial use) were better than the new type C and D. (iv) Fusel oil fermentation; Old type kokja K-23, K-26 (industrial use) and K-12 (home made) fermented high fusel oil (0.07%) and new type kokja C, D and B were the lowest (0.04%). 3. Adding food preservatives (AF-2, D.E.P.C.) and heat sterilizing of wheat bran for kokja had little concerning with the activity of new type kokja. 4. In brewing test, takju made from kokja C and D were more excellent in alcohol and fusel oil concentration than those from old type kokja K-1 and K-23. Taste and odour of takju from new type kokja was good enough to drink.

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Recovery of Mg-Ferrite Powder from Acid Leaching Mg Waste by Hydrothermal Method (산 침출 Mg폐액으로부터 수열법에 의한 Mg-Ferrite 분말회수)

  • 김영순;윤기석;안찬영;이종현;원창환
    • Resources Recycling
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    • v.6 no.4
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    • pp.31-37
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    • 1997
  • The Mg-ferrite powders were recovered from acid leaching Mg waste by hydrothermal method. Recovering conditions ofMg-Ferrite were investigated m this system and the powders prepared were characterized, using X-ray analysis, chemicalanalysis, SEM. TEM, and VSM. In this study, pH of solution and the kind of neutralizer wcre important factors on thecharacteristics of the product. The optimum condition of recovering MgPenite was the mole ratio of Fe" : Mg"=2'1, reactiontemperature : ZOWC, reaction time.lhr, at pH=lZ, and Oi partial pressure af 2000 psi. And the powders ~ccovered have amonodispersed and spherelike shapes with the narrow sue distribulion.ow sue distribulion.

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NMR peak assignment for the elucidation of the solution structure of T4 Endonuclease V

  • Im, Hoo-Kang;Hyungmi Lihm;Yu, Jun-Suk;Lee, Bong-Jin
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.183-183
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    • 1996
  • Bacteriophage T4 endonuclease V initiates the repair of ultraviolet (UV)-induced pyrimidine dimer photoproducts in duplex DNA. The mechanism of DNA strand cleavage involves four sequential stens: linear diffusion along dsDNA, pyrimidine dimer-specific binding,l pyrimidine dimer-DNA glycosylase activity, and Af lyase activity. Although crystal structure is known for this enzyme, solution structure has not been yet known. In order to elucidate the solution structure of this enzyme NMR spectroscopy was used. As a basis for the NMR peak assignment of the protein, HSQC spectrum was obtained on the uniformly $\^$15/N-labeled T4 endonuclease V. Each amide peak of the spectrum were classified according to amino acid spin systems by interpreting the spectrum of $\^$15/N amino acid-specific labeled T4 endonuclease V. The assignment was mainly obtained from three-dimensional NMR spectra such as 3D NOESY-HMQC, 3D TOCSY-HMQC. These experiments were carried out will uniformly $\^$15/N-labeled sample. In order to assign tile resonance of backbon atom, triple-resonance theree-dimensional NMR experiments were also performed using double labeled($\^$15/N$\^$13/C) sample. 3D HNCA, HN(CO)CA, HNCO, HN(CA)HA spectra were recorded for this purpose. The results of assignments were used to interpret the interaction of this enzyme with DNA. HSQC spectrum was obtained for T4 endonuclease V with specific $\^$15/N-labeled amino acids that have been known for important residue in catalysis. By comparing the spectrum of enzyme*DNA complex with that of the enzyme, we could confirm the important role of some residues of Thr, Arg, Tyr in activity. The results of assignments were also used to predict the secondary structure by chemical shift index (CSI).

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AN EVALUATION OF MECHANICAL PROPERTIES OF LIGHT-CURED RESTORATIVE COMPOSITES BY INDENTATION AND COMPRESSION TEST (압입과 압축에 의한 광중합형 수복용 복합레진의 기계적 성질 평가)

  • Lee, Yong-Woo;Yu, Mi-Kyung;Cho, Young-Gon;Bae, Tae-Sung;Lee, Kwang-Won
    • Restorative Dentistry and Endodontics
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    • v.24 no.3
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    • pp.511-518
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    • 1999
  • This study was performed to evaluate the mechanical properties of light-cured restorative composites by compression and indentation tests. Five commercially available light-activated composites (Heliomolar : HM, Aelitefil : AF, Amelogen : AG, Clearfil AP-X : CF, Z100 ZH) were used Disc specimens of 12mm in diameter and 1 mm in thickness and Cylindrical specimens of 3mm in diameter and 6mm in length were prepared for the indentation and compressive test, respectively. All specimens were immersed in distilled water at $37^{\circ}C$ for 30 days. An indentation test of 68-degree trigonal diamond pyramid was made under 10 g load for 15 seconds and an Knoop indentation test was made under 50 g load for 15 seconds. Hardness numbers, characteristic indentation depths and permanent deformation were measured during indentation of the 68-degree trigonal diamond pyramid compressive test was carried out at a crosshead speed of 0.5mm/min. The results obtained were summarized as follows, 1. The highest hardness value was obtained in the CF group and the lowest value was obtained in the HM group. Hardness values showed no significant, difference between AG group and HM group but other groups showed the significant differences in each group(p<0.05). 2. Hardness number by Knoop pyramid were higher than those of 68-degree trigonal diamond pyramid. 3. Plastic deformation during the indentation of 68-degree trigonal diamond pyramid was the lowest in the CF group and the largest in the HM group. Results of Tukey test showed the significant difference between CF group and others; also between ZH and AF groups and AG and HM groups(p<0.05) 4. The highest compressive strength was obtained in the CF group and the lowest compressive strength was obtained in the ZH group. Compressive strength values showed no significant difference between CF group and ZH group but other groups showed the significant differences in each group(p<0.05).

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Reduction leaching of manganese nodule with copper matte (동매트를 이용한 망간 단괴의 환원 침출)

  • 한오형;최경수
    • Resources Recycling
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    • v.4 no.3
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    • pp.26-31
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    • 1995
  • The leaching characteristics of manganese nodules were investigated in dilute hydrochloric acid solution using copper malie as a reductant. Thc capper matte has been found to be an effective reductant for exhacting morc than 96% of Mn, 95% of Ni, 91% of Ca, 88% of Cu and 36% of Fe when leached in 2.5 M HCI at 70$^{\circ}$C for 2hr. The dissolutions of Mu, Co, and Ni depend on thc amaunt of added cappcr matte. 7he ratin of liquid and solid is an important [actor on the extraction of metals during leaching The dissolution af Mn, Co, Ni and Cu incrcascd w~th the increase in temperalure of leachant. The leaching rates of Mn, Co, NI and Cu from manganese nodule m the presznce of copper matte is limited by bath thc surface chemical reaction and pare diffusion processes. Thc activation energies far Mn. Co: Ni and Cu were 17. 61, 12.8, 17.2 and 57.88 KcaUmol, rcspcctively.

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Ribgrass Mosaic Tobamovirus Occurred on Chinese Cabbage in Korea

  • Kim, Jeong-Soo;Cho, Jeom-Deog;Choi, Hong-Soo;Lee, Soo-Heon;Choi, Gug-Seoun;Lee, Sang-Yong;Kim, Hye-Jeong;Yoon, Moo-Kyoung
    • The Plant Pathology Journal
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    • v.26 no.4
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    • pp.328-339
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    • 2010
  • A tobamovirus, Ribgrass mosaic virus (RMV), was identified newly from chinese cabbage (Brassica campestris L. pekinensis) in Korea. Virus disease incidence of RMV on chinese cabbage was 37.9% in alpine area on August in 1993. RMV induced the symptoms of necrotic ring spots, necrotic streak on midrib and malformation. RMV, Ca1 and Ca3 isolate, could infect 35 species out of 45 plants including Chenopodium amaranticolor. Physical properties of RMV Ca1 isolate were very stable as 10.8 over for dilution end point, $95^{\circ}C$ for temperature inactivation point and 18 weeks for longevity in vitro. RMV had the soil transmission rate of 75.0% for the chinese cabbages, 'Chunhawang' and 'Seoul' cultivars. The purified virions of RMV had the typical ultraviolet absorption spectrum of maximum at 260 nm and minimum at 247 nm. RMV of Ca1 isolate was related serologically with antisera of Tobacco mosaic virus (TMV)-Cym, TMV-O and Pepper mottle virus, but not related with antiserum of Odontoglossum ring spot virus. coat protein gene of RMV-Ca1, sized 473 nucleotides, encoded 158 amino acid residues. Nucleotide identity of RMV-Ca1 CP gene was 96.4% with RMV-Shanghai (GenBank accession No. of AF185272) from China and 96.0% with RMV-Impatiens (GenBank accession No. of AM040974) from Germany. Identity of amino acids between RMV-Ca1 and the two RMV isolates was 96.8%. Specific three primers were selected for rapid and easy genetic detection of RMV using Virion Captured (VC)/RT-PCR method.

Controlling Factors of Particle Size Distribution during Formation of Cubic and Colloidal Calcium Carbonate Compounds (Cubic형과 Colloid형 탄산칼슘 합성에서의 입경제어 연구)

  • Ahn, Ji-Whan;Park, Chan-Hoon
    • Resources Recycling
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    • v.5 no.3
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    • pp.65-72
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    • 1996
  • Colloidal calcium wrbonate(diametcr 0.02-0 09 m~wja s developed to maintain the mamenl of pnriide formatio~>w ~lhoutsurlace trealment. The control factors of particle size and optimum condiliuna for compound fam*tition has not bccn studiedyet. This shldy war aimed at developing a method fur compounding colloidal calcium carbonfcte to cnl~hol cubic calciumcarbonate, and then compounding the b-o types oI precipitated calcium wrbonatc under optimum wndilrans Calc~umhydroxide was calcinated at 1, lWC far two hours, md then hydrated for 30 minutes at t i i O rprn and ambiznt temperahlle.Two-liter suspension was subjected to the contact with carbon dioxide at l5"C, 600 ipxn and C0= injection in the rate of 1 Umin Two types of dcium carbonate(cuhic calcium carbonatc(0 24.9 pm) md collnidd calcium mhnnate (0.02-0 09 pm))were compounded by "wing the concentrations of calcium oxide and ihe suspension were compounded. It was found that theoptimum concentrations of each suspensions were 5 wt % and 2.5 \I*.% respectively. ' h c key control factor af thc parlicle slzcdislribution was the concenkation al the suspension. The size of compounded particles was measured by a Zcla S k r 'fieaverage particle size of the cubic calcium carbonate aas 223.4 nm(0.223 pm), and that of thc colloidal a~lciumc arbonate was93.6 nm (0.093 km). Ihe particle sizc was evenly cantlolled on a stdblc basis in an H, O reaction system.asis in an H, O reaction system.

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Test methodology of acceleration life test on feeder cable assembly (Feeder Cable Assembly의 가속수명시험법 개발)

  • Han, Hyun Kak
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.62-68
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    • 2016
  • The feeder cable assembly is an automotive part used for telecommunication. If it malfunctions, the control and safety of the automobile can be put at risk. ALT (Accelerated Life Testing) is a testing process for products in which they are subjected to conditions (stress, strain, temperatures, etc.) in excess of their normal service parameters in an attempt to uncover faults and potential modes of failure in a short amount of time. Failure is caused by defects in the design, process, quality, or application of the part, and these defects are the underlying causes of failure or which initiate a process leading to failure. Thermal shock occurs when a thermal gradient causes different parts of an object to expand by different amounts. Thermal shock testing is performed to determine the ability of parts and components to withstand sudden changes in temperature. In this research, the main causes of failure of the feeder cable assembly were snapping, shorting and electro-pressure resistance failure. Using the Coffin-Manson model for ALT, the normal conditions were from Tmax = $80^{\circ}C$ to Tmin = $-40^{\circ}C$, the accelerated testing conditions were from Tmax = $120^{\circ}C$ to Tmin = $-60^{\circ}C$, the AF (Acceleration Factor) was 2.25 and the testing time was reduced from 1,000 cycles to 444 cycles. Using the Bxlife test, the number of samples was 5, the required life was B0.04%.10years, in the acceleration condition, 747 cycles were obtained. After the thermal shock test under different conditions, the feeder cable assembly was examined by a network analyzer and compared with the Weibull distribution modulus parameter. The results obtained showed good results in acceleration life test mode. For the same reliability rate, the testing time was decreased by a quarter using ALT.

Effects of simultaneous supplementation of laying hens with α-linolenic acid and eicosapentaenoic acid/docosahexaenoic acid resources on egg quality and n-3 fatty acid profile

  • Zhang, Pingping;Tang, Chuanqiu;Ding, Zongqing;Huang, Hui;Sun, Yong
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.7
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    • pp.973-978
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    • 2017
  • Objective: The aim of this study was to investigate the effects of simultaneous supplementation of laying hens with alpha-linolenic acid (ALA) resources (flax, perilla, and Eucommia ulmoides [E. ulmoides] seeds) and eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) resources (Schizochytrium sp.) on egg quality and n-3 polyunsaturated fatty acids (PUFA) profile. Methods: Dietary treatments were as follows: i) diet C (control diet); ii) diet F (diet C+10% flaxseeds); iii) diet P, (diet C+10% perilla seeds); iv) diet E (diet C+10% E. ulmoides seeds); v) diet A (diet C+1.5% microalage); vi) diet AF (diet C+10% flaxseeds+1.5% microalage); vii) diet AP (diet C+10% perilla seeds+1.5% microalgae); viii) diet AE (diet C+10% E. ulmoides seeds+ 1.5% microalage). Results: Egg weight, yolk weight and production ratio were not significantly affected by either algae or in combination with seeds (p>0.05). No significant difference was observed in ALA and DHA concentration in eggs between flaxseed, perila, and E. ulmodies seeds supplementation alone (p>0.05). N-3 PUFA in eggs was slightly improved by microalgae supplementation. The best supplementation, a combination of microalgae and perilla seeds, elevated (p<0.05) ALA from 19.7 to 202.5 mg/egg and EPA+DHA from 27.5 to 159.7 mg/egg. Highest n-3 PUFA enrichment (379.6 mg/yolk) was observed with supplementation of a combination of perilla seed and microalgae (362.2 mg/yolk), followed by a combination of flaxseed and microalgae (348.4 mg/yolk). The ALA, EPA, and DHA content obtained with a combination of microalgae and seeds surpassed the total sum of that obtained with microalgae or ALA-seeds alone. Conclusion: It is feasible to enrich eggs with n-3 PUFAs by perilla or E. ulmodies seeds instead of flaxseeds. Simultaneous supplementation of microalgae and seeds helped improve the transfer from EPA and docosapentaenoic acid into DHA.

Molecular Evidence of Recombination on Korean Isolates of Tomato yellow leaf curl virus by Nucleotide Transversions and Transitions

  • Lee, Hye-Jung;Park, Jung-An;Auh, Chung-Kyoon;Lee, Kyeong-Yeoll;Kim, Chang-Seok;Lee, Gwan-Seok;Soh, Hyun-Cheol;Choi, Hong-Soo;Lee, Suk-Chan
    • The Plant Pathology Journal
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    • v.27 no.4
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    • pp.378-384
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    • 2011
  • Tomato yellow leaf curl virus (TYLCV), a member of genus Begomovirus, was isolated in Korea in 2008. We sequenced and analyzed the DNA-A of 51 TYLCV isolates from Korea, and 13 of the TYLCV isolates were selected as type representatives of TYLCV from six Korean provinces. The 13 TYLCV isolates were classified into Korea Group 1 (KG1, nine isolates) and Korea Group 2 (KG2, four isolates) based on the results of phylogenetic analysis and genome size (2774 and 2781 nucleotides, respectively). A recombination detection program 3 (RDP3) revealed two recombinations between the TYLCV Korea isolates and other TYLCV isolates [Thailand (AF206674), Iran (AJ132711), and Israel (X76319)]. TYLCV Jeju isolate was characterized by two recombination events (E1 and E2) caused by the presence of E1 in ORF V1 and C3, which may seem to be the mutations of the high nucleotide transversion and transition rate. Collectively, our results suggest that the occurrence of nucleotide transversions and transitions in TYLCV DNA-A might have induced novel recombination events within the TYLCV Korea isolates.