• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.524-529
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• 2014

Two methods of feed preference trials were compared to evaluate the acceptability of 5 fresh foliages: Leucaena leucocephala, Moringa oleifera, Portulaca oleracea, Guazuma ulmifolia, and Brosimum alicastrum that was included as control. The evaluation included chemical analyses and forage intake by rabbits. The first method was a cafeteria trial; 12 California growing rabbits aged 8 wk, allocated in individual cages, were offered the five forage plants at the same time inside the cage, while in the second trial 60 California growing rabbits aged 8 wk, allocated individually, were randomly distributed into 5 experimental groups (n = 12/group); for each group just one forage species was offered at a time. The testing period for each method lasted for 7 d, preceded by one week of adaptation. The results showed that B. alicastrum and L. lecocephala were the most preferred forages while on the contrary G. ulmifolia was the least preferred one by rabbits. The results also revealed that the CV% value for the 2nd method (16.32%), which the tested forages were presented separately to rabbits, was lower and methodologically more acceptable than such value for the $1^{st}$ method (34.28%), which all forages were presented together at the same time. It can be concluded that a range of tropical forages were consumed in acceptable quantities by rabbits, suggesting that diets based on such forages with a concentrate supplement could be used successfully for rabbit production. However, growth performance studies are still needed before recommendations could be made on appropriate ration formulations for commercial use.

• Journal of Acupuncture Research
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• 제18권3호
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• pp.79-93
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• 2001

Objective : This study was purposed to investigate the anti-cancer and anti-metastasis and immune response of Aqua-acupuncture with Amomum amarum Lourerio infusion solution. Methods : The Amomum amarum Lourerio infusion solution put into Chung-wan(CV12) of BALB/c or C57BL6 mice were rised to cancer by B15-F10 and HT1080, S-180 cancer cell line. Results : The following result have been obtained 1. The effect on expression of MMP-9 gene about the HT1080 cancer cell line was increased in 100, $50{\mu}g/m{\ell}$ diluent groups, compared with control group. 2. The effect on expression of MMP-9 gene about the B15-F10 cancer cell line was increased in all the sample groups, compared with control group. 3. S-180 cancer cell line transplants in BALB/c mice were inhibited significantly in weight inctease in all the sample groups, compared with control group. 4. The effect on spleen cell proliferation was decreased in all the sample groups, compared with control group. 5. The $IFN-{\gamma}$ in all the sample groups and the $TNF-{\alpha}$ in 25 and $12.5{\mu}g/m{\ell}$ diluent groups was increased. 6. In flow cytometry, the number of CD4+, CD19+ cell in all the sample group was increased and the number of CD8+ cell in $100{\mu}g/m{\ell}$ diluent group, compared with control group. Conclusion : According to the result, Aqua-acupuncture with Amomum amarum Lourerio infusion solution has significant anti-cancer, anti-metastasis and Immune response improvement.

• 한국작물학회지
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• 제48권3호
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• pp.147-151
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• 2003

제주지역에서 상위급 씨감자 가을채종재배 시 폴리에칠렌(PE)멀칭이 감자의 생육 및 수량성에 미치는 효과를 구명하고자 일반종서, 경삽 및 소서 플러그묘를 2001년 8월 20일에 직파 또는 정식한 후 무멀칭, 투명 및 흑색 PE멀칭재배를 하여 얻어진 결과를 요약하면 다음과 같다. 1. 주당 총서수는 멀칭처리에 큰 영향을 받지 않고 4.6개 내외였으나, 종묘종류별로는 직파구에서 5.14개로 가장 많았고 경삽묘 및 소서묘정식구에서는 각각 4.15, 5.15개였다. 2. 총서수량은 무멀칭구에서 21.16톤/ha이었는데, 투명 및 흑색 PE멀칭에 의하여 각각 16, 8% 증수되었고, 경삽묘정식구가 가장 많았으며 다음으로 소서묘, 직파구 순이었다. 3. 평균서중은 무멀칭구에서 평균 72.2g이었고 투명 및 흑색 PE멀칭에 의하여 각각 14, 12%증가되었는데, 멀칭효과는 경삽묘정식구에서 가장 컸었다.

• 식물조직배양학회지
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• 제26권1호
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• pp.65-69
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• 1999

LSV 제거 효과를 조사하기 위하여 LSV에 감염된 나리 Casa Blanca를 재료로 기내 배양 중에 화학약제 및 열처리를 단독 또는 병형처리하였다. 단독처리시 ribavirin 20 mg/L과 열처리 35$^{\circ}C$/$25^{\circ}C$ (주간/야간)에서 식물의 생육 및 LSV 제거율(각각 86%, 80%)이 ekfs 처리보다 좋았다. 이 두 처리를 병행하였을 경우, 열처리 기간이 길수록 LSV 제거율은 증가하는 경향이었으며, 열처리 16주의 모든 처리에서 ribavirin 농도와 관계없이 100%의 LSV 제거 효과를 보였다. 토양 정식 후 LSV 무병화 정도는 ribavirin 20 mg/L 농도와 열처리 8주 병행처리에서 12개체 중 8개체가 무병화를 보여 가장 효과적이었으며, 전체 조사 수를 기준으로 했을 때 약 21% 정도의 무병화가 유지되었고, 나머지는 토양의 자연 조건에서 다시 바이러스의 복제, 증식이 되는 것으로 보인다.

• 한국작물학회지
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• 제49권1호
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• pp.12-18
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• 2004

Activity of senescence-induced antioxidant enzymes in the detached rice seedlings (Oryza sativa L. cv. Dongjin) was examined. The levels of $\textrm{H}_2\textrm{O}_2$ content and peroxidase (POD) activity were gradually increased during leaf senescence, whereas catalase activity was decreased. The activity of superoxide dismutase (SOD) was increased, and ascorbate peroxidase (APX) and glutathione reductase (GR) were slightly increased until 3d and 4d of dark induced-senescence, and thereafter were decreased. The activation of all SOD isoforms showed a significant decrease after 6d and 7d. After 4d to 7d of dark senescence, there was a significant effect in enhancing the activity of APX-12 and -13 isoforms as compared with light, despite similar levels in total APX activity. GR-8 and -10 isoforms were more effective in leaf senescence at 4d to 7d, particularly with respect to dark-induced senescence. These results suggest that the metabolism of active oxygen species such as $\textrm{H}_2\textrm{O}_2$ is dependent on various functionally interrelated antioxidant enzymes such as catalase, peroxidase, SOD, APX and GR.

• 식물병연구
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• 제12권3호
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• pp.278-283
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• 2006

국내 배추 재배지에서 큰 문제가 되고 있는 뿌리혹병을 석회질소로 방제하는 방법을 구명하기 위하여, 고령지농업연구소의 뿌리혹병 다발포장에서 $2002{\sim}2003 $년에 걸쳐 시험을 실시하였다. 석회질소의 처리량은 배추에 대한 질소질 비료 기비량과 같은 61 kg/10a처리할 때, 33%의 발병도를 보여 대조약제인 후루설파마이드의 77%에 비해 우수한 방제 효과를 보였다. 석회질소를 배추 정식 전 5, 10, 15, 20일에 처리한 결과, 정식 5일전 처리가 48%의 발병도를 보여 후루설파마이드 분제의 67%에 비해 발병 억제 효과가 인정되었다. 석회질소와 요소비료를 이용하여 재배한 배추의 수확기 질소 흡수량은 요소가 17.8kg/10a, 석회질소가 17.6kg/10a으로 유의성이 없었다. 이와 같은 결과로 볼 때 석회질소는 질소질 비료로서 뿐만 아니라 배추뿌리혹병을 방제하기 위한 화학약제의 대용으로서 사용될 수 있을 것으로 생각된다.

• 한국음향학회지
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• 제13권1호
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• pp.71-83
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• 1994

본 논문은 한국어 문어면환(TTS : Text-to-Speech) 시스템내에서의 음성합성시 음질 및 자연성 개선을 위한 연구 결과이다. 합성방법으로는 단음절단위의 파형을 시간영역에서 분석(표1)하여 규칙합성에 필요한 매개변수(표2)를 추출하여 규칙합성시켰다. 실험에 사용된 음절은 한국어 발음 대사전의 빈도순위에 따라 V형 19개, CV형 80개, VC형 30개, CVC형 100개등 총 229음절을 선정하여 규칙합성시켰다. 규칙합성음의 평가방법으로는 229개의 규칙합성음중 음절 유형별로 15개씩 무작위로 추출한 합성음을 사전지식이 없는 임의의 그룹을 선정하여 이해도, 명료도, 잡음감, 자연성등 4가지 항목에 대하여 주관적인 오피니온 평가를 수행하였다. 실험결과, 합성음의 음질은 대단히 명료한 수준이었으며, 운율요소의 제어결과는 지속시간(장단)과 악센트(강약)의 제어(그림 9, 그림 10)가 가능하였으며, 피치주기(억양)의 제어도 Lagrange 보간법을 사용함으로써 가능하였다(그림 11, 그림 12).

• The Journal of Korean Physical Therapy
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• 제21권2호
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• pp.109-116
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• 2009

Purpose: $17\beta$-estradiol is the most active endogenous estrogen, which is related to favorable changes in the plasma lipid profile, to relaxation of the coronary vessels, and to a decrease in platelet aggregation and vascular smooth muscle cell migration. However, although the beneficial effect of estrogens on plasma lipoproteins (ie, lowering low-density lipoprotein and increasing high-density lipoprotein cholesterol) contributes to cardiovascular protection, it does not fully account for the protective effect, particularly in the application of physical therapy, including low frequency electrical stimulation. Methods: The aim of this study was to demonstrate the inhibition of stressors, such as endothelin-1 (ET-1), serotonin (5-hydroxytryptamine, 5-HT), prostaglandin $F2\alpha$ ($PGF2\alpha$), and a protein kinase C (PKC) activator 12-deoxyphorbol 13-isobutyrate (DPB), induced isometric tension by $17\beta$-estradiol in vascular smooth muscle strips, respectively. In addition, the effects of low frequency electrical stimulation at the meridian points (CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52) on the indirect antihypertensive effect were examined by monitoring the changes in the serum $17\beta$-estradiol concentration in healthy volunteers. Results: Isometric tension analysis showed that the responses of inhibited tension by $17\beta$-estradiol were similar to the same stressors in rat aortic smooth muscle strips. Furthermore, although the continued amplitude modulation (AM) type of electrical stimulation was not increased significantly by electrical stimulation, the current of the frequency modulation (FM) type of low frequency electrical stimulation increased the serum $17\beta$-estradiol concentration in normal volunteers. Conclusion: These results, in part, suggest that $17\beta$-estradiol has the capacity to supress stressor-induced muscle tension, and electrical stimulation, particularly current of the FM type, has a modulatory effect on the sex steroid hormones, particularly $17\beta$-estradiol, in healthy volunteers.

• 한국작물학회지
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• 제43권3호
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• pp.157-160
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• 1998

To find out the effects of humidification and hardening of rice (Oryza sativa L. cv. Ilpumbyeo) seeds on the germination, both normal and artificially aged seeds with 60% germination rate were humidified at 40, 60, and 80% RHs and $25^{\circ}C$ for five weeks or hydrated for 6, 12, 18, and 24 hours at $25^{\circ}C$ from one to five cycles for hardening. Relative humidity and duration of humidification did not affect the germination rate of normal seeds, while reduced the time to get 50% germination rate ($T_{50}$) by 1.0～1.6 days compared to that of normal seeds at $25^{\circ}C$. Aged seeds humidified at 40 and 60% RHs did not affect the germination rate and $T_{50}$ regardless of relative humidity and duration of treatment, while at 80% RH, the germination rate decreased and $T_{50}$ increased significantly with the duration of humidification. Hardening of normal seeds reduced $T_{50}$ by 0.7～1.1 days without changes in the germination rate. However, the germination rate and $T_{50}$ of aged seeds soaked in water for 6, 12, and 18 hours were similar regardless of soaking/drying cycles, while hardening of artificially aged seeds with 1～4 cycles in 24 hours soaking increased the germination rate by 11-16% and reduced $T_{50}$ by 1.4～2.0 days.

• Journal of Plant Biology
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• 제29권1호
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• pp.41-51
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• 1986

The plants of Nicotiana tabacum L. cv. NC2326 were germinated in 10 cm D$\times$20 cm H polyethylene pot, and sand-cultured with Hoagland solution near by the window of laboratory room(26$\pm$5$^{\circ}C$). The growing plants were sprayed with various concentrations of ABA around 9 : 00 a.m. once in every two days for 12 weeks in summer. As the results, frequency of stomatal number, stomatal opening, chlorophyll content, respiration rate, and protein content in the leaves were decreased with the increasing of concentrations of ABA, respectively. The plant growth was inhibited by exogenous ABA, but leaf abscission was not found during the experimental period. The ratio of three to one in chlorophyll a to b was not altered by exogenous ABA. All the stomata were closed within three minutes by 100 $\mu\textrm{g}$ ml-1 ABA and within seven minutes by 1-10 $\mu\textrm{g}$ ml-1 ABA after the spraying of ABA, and then reopended after a few hours in 1-10 $\mu\textrm{g}$ ml-1 ABA and after 24 hours in 100 $\mu\textrm{g}$ ml-1 ABA. The polar movement of chloroplast within the guard cells was found in the higher concentrations of 10 and 100 $\mu\textrm{g}$ ml-1 ABA, but not found in the lower concentrations than 1 $\mu\textrm{g}$ ml-1 ABA. During the night and weak light, it was fond that the inhibition of respiration rate by the higher concentration of ABA was owing to firstly the stomatal closure by the spraying of ABA and secondly the decrease of stomatal frequency by the inhibition of stomatal development with exogenous ABA for the long period of 12 weeks. In the band number of leaf protein by the electrophoresis, most of the protein bands were disappeared by the higher concentration of 100 $\mu\textrm{g}$ ml-1 ABA, but were not altered by the lower concentration of ABA in comparison with control.