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Accurate Analysis of Chromium in Foodstuffs by Using Inductively Coupled Plasma Mass Spectrometry with a Collision-Reaction Interface

  • Lee, Seung Ha;Kim, Ji Ae;Choi, Seung Hyeon;Kim, Young Soon;Choi, Dal Woong
    • Bulletin of the Korean Chemical Society
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    • v.34 no.6
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    • pp.1689-1692
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    • 2013
  • Food is a common source of chromium (Cr) exposure. However, it is difficult to analyze Cr in complex food matrices by using inductively coupled plasma mass spectrometry (ICP-MS) because the major isotope, $^{52}Cr$, is masked by interference generated by the sample matrix and the plasma gas. Among the systems available to minimize interference, the recently developed collision-reaction interface (CRI) has a different structure relative to that of other systems (e.g., collision cell technology, octopole reaction system, and dynamic reaction cell) that were designed as a chamber between the skimmer cone and quadrupole. The CRI system introduces collision or reaction gas directly into the plasma region through a modified hole of skimmer cone. We evaluated the use of an CRI ICP-MS system to minimize polyatomic interference of $^{52}Cr$ and $^{53}Cr$ in various foodstuffs. The $^{52}Cr$ concentrations measured in the standard mode were 2-3 times higher than the certified values. This analytical method based on an ICP-MS system equipped with a CRI of helium gas was effective for Cr analysis in complex food matrices.

A Design for Improving Performance of Dynamic Traffic in High Rate WPAN (고속 WPAN의 동적 트래픽 성능 개선을 위한 구조 설계)

  • Kim, Ji-Eun;Lee, Sang-Jae;Jeon, Young-Ae;Choi, Sang-Sung
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 2005.11a
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    • pp.121-124
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    • 2005
  • The High Rate Wireless Personal Area Networks (WPAN) is mainly targeted to consumer electronics and portable communication devices which need high rates and QoS. To achieve these goals, the WPAN provides data rates up to 1Gbps and adopts a Time Division Multiple Access (TDMA) MAC protocol. Since IEEE 802.15.3 MAC is based on TDMA scheme, it has good performance in dealing with real traffics. But it does not give better results for dynamic traffic. For dynamic traffic, IEEE 802.15.3 MAC needs a kind of request and response policy that brings about the degradation of performance in order to adapt to network changes. To overcome this problem and enhance the data throughput, this paper proposes a superframe structure which uses the Enhanced Contention Access Period (ECAP) to transmit data during sleep-CTA in PM mode.

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Estimation of Polarization Ratio for Sea Surface Wind Retrieval from SIR-C SAR Data

  • Kim, Tae-Sung;Park, Kyung-Ae
    • Korean Journal of Remote Sensing
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    • v.27 no.6
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    • pp.729-741
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    • 2011
  • Wind speeds have long been estimated from C-band VV-polarized SAR data by using the CMOD algorithms such as CMOD4, CMOD5, and CMOD_IFR2. Some SAR data with HH-polarization without any observations in VV-polarization mode should be converted to VV-polarized value in order to use the previous algorithms based on VV-polarized observation. To satisfy the necessity of polarization ratio (PR) for the conversion, we retrieved the conversion parameter from full-polarized SIR-C SAR image off the east coast of Korea. The polarization ratio for SIR-C SAR data was estimated to 0.47. To assess the accuracy of the polarization ratio coefficient, pseudo VV-polarized normalized radar cross section (NRCS) values were calculated and compared with the original VV-polarized ones. As a result, the estimated psudo values showed a good agreement with the original VV-polarized data with an root mean square error by 0.99 dB. We applied the psudo NRCS to the estimation of wind speeds based on the CMOD wind models. Comparison of the retrieved wind field with the ECMWF and NCEP/NCAR reanalysis wind data showed relatively small rms errors of 1.88 and 1.91 m/s, respectively. SIR-C HH-polarized SAR wind retrievals met the requirement of the scatterometer winds in overall. However, the polarization ratio coefficient revealed dependence on NRCS value, wind speed, and incident angle.

Strength Characteristics and Non-Destructive Evaluation of Composites with Heat Damage (국부열손상을 받은 복합재료의 강도특성 및 비파괴평가)

  • Nam, Ki-Woo;Kim, Young-Un
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2002.05a
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    • pp.173-178
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    • 2002
  • Fourier transform has been one of the most common tools to study the frequency characteristics of signals. With the Fourier transform alone, it is difficult to tell whether signal's frequency contents evolve in time or not. Except for a few special cases, the frequency contents of most signals encountered in the real world change with time. Time-frequency methods are developed recently to overcome the drawbacks of Fourier transform, which can represent the information of signals in time and frequency at the same time. In this study, heat damage process of a carbon fiber reinforced plastic(CFRP) and glass fiber reinforced plastic(GFRP) under monotonic tensile loading was characterized by acoustic emission. Different kinds of specimens were used to determine the characteristics of Strength and AE signals. Time-frequency analysis methods were employed for the analysis of fracture mechanism in CFRP such as matrix cracking, debonding and fiber fracture.

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Identification of a novel type of small molecule inhibitor against HIV-1

  • Kim, Byung Soo;Park, Jung Ae;Kim, Min-Jung;Kim, Seon Hee;Yu, Kyung Lee;You, Ji Chang
    • BMB Reports
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    • v.48 no.2
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    • pp.121-126
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    • 2015
  • Here we report a new chemical inhibitor against HIV-1 with a novel structure and mode of action. The inhibitor, designated as A1836, inhibited HIV-1 replication and virus production with a 50% inhibitory concentration ($IC_{50}$) of $2.0{\mu}M$ in an MT-4 cell-based and cytopathic protection antiviral assay, while its 50% cytotoxic concentration ($CC_{50}$) was much higher than $50{\mu}M$. Examination of the effect of A1836 on in vitro HIV-1 reverse transcriptase (RT) and integrase showed that neither were molecular targets of A1836. The characterization and re-infection assay of the HIV-1 virions generated in the presence of A1836 showed that the synthesis of early RT products in the cells infected with the virions was inhibited dose-dependently, due in part to abnormal protein formation within the virions, thus resulting in an impaired infectivity. These results suggest that A1836 might be a novel candidate for the development of a new type of HIV-1 inhibitor.

Antimicrobial Activity of the Scolopendrasin V Peptide Identified from the Centipede Scolopendra subspinipes mutilans

  • Lee, Joon Ha;Kim, In-Woo;Kim, Mi-Ae;Ahn, Mi-Young;Yun, Eun-Young;Hwang, Jae Sam
    • Journal of Microbiology and Biotechnology
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    • v.27 no.1
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    • pp.43-48
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    • 2017
  • In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.

Risk Evaluation Based on the Hierarchical Time Delay Model in FMEA (FMEA에서 계층적 시간 지연 모형에 근거한 위험평가)

  • Jang, Hyeon Ae;Lee, Min Koo;Hong, Sung Hoon;Kwon, Hyuck Moo
    • Journal of Korean Society for Quality Management
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    • v.44 no.2
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    • pp.373-388
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    • 2016
  • Purpose: This paper suggests a hierarchical time delay model to evaluate failure risks in FMEA(failure modes and effects analysis). In place of the conventional RPN(risk priority number), a more reasonable and objective risk metric is proposed under hierarchical failure cause structure considering time delay between a failure mode and its causes. Methods: The structure of failure modes and their corresponding causes are analyzed together with the time gaps between occurrences of causes and failures. Assuming the severity of a failure depends on the length of the delayed time for corrective action, a severity model is developed. Using the expected severity, a risk priority metric is defined. Results: For linear and quadratic types of severity, nice forms of expected severity are derived and a meaningful metric for risk evaluation is defined. Conclusion: The suggested REM(risk evaluation metric) provides a more reasonable and objective risk measure than the conventional RPN for FMEA.

Secondary structure analysis of MRA1997 from Mycobacterium tuberculosis and characterization of DNA binding property

  • Kim, Hyo Jung;Lee, Ki-Young;Kim, Yena;Kwon, Ae-Ran;Lee, Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.20 no.2
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    • pp.36-40
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    • 2016
  • MRA1997 is a highly conserved protein from mycobacterial strains. However, no structural and functional information is associated with it. Thus, to obtain details about structure and function of this protein, we have utilized NMR spectroscopy. The recombinant MRA1997 was highly purified and its DNA binding mode was characterized. The tertiary structure of MRA1997 was modeled on the basis of our NMR chemical shift data combined with the webserver CS23D. The binding of MRA1997 with DNA was first monitored by electrophoresis mobility shift assays. The residues involved in DNA binding are identified using NMR chemical shift perturbation experiments. Based on our study, we suggest that MRA1997 interacts with DNA and may play an important role in Mycobacterium tuberculosis physiology.

Design for Vibration of Radiator at the Early Stage of Product Development Process in Construction Equipment (건설중장비 제품개발 초기 단계에서의 라디에이터 진동 설계)

  • Kang, Hyun-Seok;Kim, Sung-Hwan;Kang, Jong-Min
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2011.10a
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    • pp.271-276
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    • 2011
  • The working conditions of construction equipments such as excavators, wheel loaders and haulers are very tough and severe in fact. To design main components of construction equipment under the severe environment, it's important for engineers to consider design for vibration durability point of view at the early stage of product development process. Radiator as a cooling unit of construction equipment is one of critical components to apply design for vibration. We present a design for vibration process and methodology on the radiator system in construction equipment industries. From the natural frequency and the random vibration analysis based on field vibration test data, we could find current status of radiator layout design to develop and made best layout specification of radiator design to decide for product development process at the early stage.

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Floral Image Make-up - Centered on Georgia O'Keeffe's Paintings - (Floral Image Make-up에 관한 연구 - 조지아 오키프(Georgia O'Keeffe)의 작품을 중심으로 -)

  • Kim, Hyo-Sook;Kang, In-Ae
    • Journal of the Korean Home Economics Association
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    • v.43 no.11 s.213
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    • pp.97-107
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    • 2005
  • Ed- the file appears to be corrupted, and in many sections (these are highlighted) I cannot read it. I presume it has been copied from another format, maybe Hangul 2004. The purpose of this study was to determine a method of make-up image extraction from specific paintings and also to create cyber make-up models according to the images. For this study, Georgia O'Keeffee's floral paintings were analyzed and their colors were compiled to make color palettes. This study attempted to approach floral image make-up which applies specific paintings through the digital mode in the manner of computer graphics. The results of this study were as follows: First, we found romantic images, including feminine, lovely and soft images by Y, GY and RP group colors, in 'Two Calla Lillies on Pink'. Second, we found modem images, including urban, up-to-date and cool images by G, GY and B group colors, in 'Blue and Green Music'. Third, we found sexy images, including brilliant, tempting and daring by R, B and G group colors, in 'Music-Pink and Blue'. To summarize, the images of the paintings were similar to those of the make-up models.