• 한국융합학회논문지
• /
• 제8권7호
• /
• pp.63-73
• /
• 2017

본 융합연구의 목적은 간호사의 코칭행동수준과 감성지능이 조직유효성에 미치는 영향을 확인하기 위한 서술적 조사연구이다. 연구대상자는 B, U광역시 4개의 종합병원 간호사이며, 2017년 3월 2일부터 2017년 3월 23일까지 자기기입식 설문지로 조사 후 최종 182부를 IBM SPSS 21.0프로그램을 이용하여 분석하였다. 연구결과 코칭행동수준 $3.28{\pm}0.42$점, 감성지능 $3.27{\pm}0.45$점, 조직유효성 중 조직몰입 $2.89{\pm}0.52$점, 직무만족도 $2.94{\pm}0.53$점, 이직의도 $3.24{\pm}0.56$점이었다. 조직몰입은 코칭행동수준, 감성지능과 정적상관관계가 있었고, 조직유효성에 영향을 미치는 융합요인은 프리셉터 경험(${\beta}=.25$, p=.001), 간호직 만족도(${\beta}=.26$, p<.001), 코칭행동수준(${\beta}=.26$, p<.001)이었고 설명력은 25%이었다. 따라서 간호사의 조직몰입을 높이기 위해서 직무의 특성을 만족시키고, 코칭행동수준을 향상시키는 중재프로그램이 마련되어 적용된다면 조직유효성을 높일 수 있을 것으로 기대한다.

• 디지털융복합연구
• /
• 제17권5호
• /
• pp.407-416
• /
• 2019

본 연구는 보건계열 대학생들의 학습전략에 영향을 미치는 요인들을 분석하고자 하였다. 자료수집은 J도 소재의 2개 대학에 재학 중인 보건 의료분야 전공 대학생 373명을 대상으로 자가보고식 설문지를 통해 이루어졌으며, 수집된 자료는 SPSS/WIN 23.0 프로그램을 이용하여 분석하였다. 연구결과 학습 전략은 진학동기, 전공 만족도에 따라 유의한 차이가 있었으며, 학업적 자기 효능감과 자기주도적 학습은 학년, 진학동기, 전공만족도에 따라 유의한 차이가 있었다. 학습전략과 독립변수 간 상관계수는 학업적 자기 효능감(r=.478, p<.001), 자기주도적 학습(r=645, p<.001), 학업 스트레스(r=-.193, p<.001)로 유의하였다. 자기주도적 학습(${\beta}=0.61$), 전공만족도(만족)(${\beta}=0.31$), 전공만족도(보통)(${\beta}=0.24$)의 순으로 학습전략에 영향을 주며, 전체 설명력은 42.6%였다. 연구 결과를 토대로 전공만족도 개선방안과 자기주도적 학습능력 향상방안의 모색은 보건계열 대학생들의 학습전략을 높이는데 긍정적 영향을 줄 수 있을 것이다.

• 가정∙방문간호학회지
• /
• 제26권1호
• /
• pp.64-71
• /
• 2019

Purpose: This study investigated the factors affecting spirituality in nursing students. Method: A total of 152 nursing students participated in the study. Data collection was conducted from October to November 2018. The data were analyzed using descriptive statistics, t-tests, analysis of variance, Pearson's correlation coefficient, and multiple stepwise regression via SPSS WIN 25.0. Results: The mean scores for empathy, gratitude, positive affect, negative affect, and spirituality were all average. However, there were significant differences in mean scores for spirituality according to satisfaction with major (F=5.88, p=.003), study grade (F=10.17, p<.001). Spirituality was found to be correlated with fantasy (r=.17, p<.001), empathic concern (r=.41, p<.001), perspective taking (r=.42, p<.001), personal distress (r=-.31, p<.001), gratitude (r=.65, p<.001), positive affect (r=.52, p<.001), and negative affect (r=-.30, p<.001). Significant predictors of spirituality included gratitude (${\beta}=.38$, p<.001), study grade (${\beta}=.34$, p=.009), positive affect (${\beta}=.29$, p<.001), and perspective taking (${\beta}=.16$, p=0.37), one of the sub-dimensions of empathy. Conclusion: To improve spirituality among nursing students, nursing educators should develop educational intervention programs to promote gratitude, study grades, positive affect, and perspective taking.

• Journal of Chest Surgery
• /
• 제43권6호
• /
• pp.588-595
• /
• 2010

배경: 종양의 종류에 따라 발현되는 종양 항원의 종류도 다양하며 그 발현률의 차이는 더욱 다양하다. 이와 같은 이유로 폐암의 치료연구를 위해서는 인체를 대신할 만한 동물 모형이 절대 필요하다. 저자는 편평상피세포암의 세포주를 배양하여 Nude mice의 흉강 내에 주입하는 방법으로 종양형성을 시도하여 종양형성의 성공 유무와 조직학적 변화와 함께 폐암과 관련 있는 EGFR (epidermal growth factor receptor)의 수용기 중 하나인 HER2/neu 종양 유전자와 세포 성장 억제 작용과 악성화 과정에서의 저항성으로 작용하는 TGF-${\beta}_1$을 각각 정량 하도록 하여 인공적인 동소 폐암의 경우 암유전자의 발현에 관하여 조사해보고자 하였다. 대상 및 방법: 면역성이 없는 20마리의 수컷생쥐 (Male BALB/c nude mice)로 5마리를 대조군으로 하였으며, 나머지 15마리는 실험군으로 하고 체중은 20~25 gm (Orient, Japan)의 범위에 있었다. HER2/neu는 채혈하여 보관된 혈액의 혈청을 분리하여 CLIA (chemiluminiscent immunoassay) 법으로 정량적으로 측정하였으며 TGF-${\beta}_1$은 immunosandwitch법을 이용하여 정량 분석하였다. 통계학적 분석을 위하여 SPSS통계(SPSS Version10.0, USA)프로그램을 이용하였으며 Student T test를 하였으며 p값이 0.05 미만인 경우를 유의성이 있는 것으로 간주하였다. 결과: 정상 대조군에서나 인위적으로 주입한 폐암 세포주에 의한 폐암이 만들어진 이후에도 HER2/neu 유전자의 증폭은 전혀 반응을 나타내지 못하였다. 하지만 TGF-${\beta}_1$ 대조군의 정량치는 $28,490{\pm}8,549pg/mL$이었고 폐암 세포 주입군은 $42,362{\pm}14,449pg/mL$로 유의하게 1.48배 높게 나왔다(p<0.483). HER2/neu 유전자와 TGF-${\beta}_1$은 유의한 상관관계가 없는 것으로 나타났다. 결론: TGF-${\beta}_1$유전자는 대조군에 비하여 1.48배 정도의 증폭이 발현되었다. TGF-${\beta}_1$의 증폭은 Nude mice에서 발암에 의한 생체 치유 억제 기전이 확실히 작동하고 있다는 것을 의미하며, 인체의 조기 폐암의 발견에 역할을 가능케 하는 정량 검사법으로 이용할 수도 있을 것이다.

• Archives of Pharmacal Research
• /
• 제27권9호
• /
• pp.954-960
• /
• 2004

Expression of the NF-$textsc{k}$B-dependent genes responsible for inflammation, such as TNF-$\alpha$, IL-1$\beta$, and nitric oxide synthase (NOS), contributes to chronic inflammation which is a major cause of neurodegenerative diseases (i.e. Alzheimer＇s disease). Although NF-$textsc{k}$B plays a biphasic role in different cells like neurons and microglia, controlling the activation of NF-$textsc{k}$B is important for its negative feedback in either activation or inactivation. In this study, we found that ursodeoxycholic acid (UDCA) inhibited I$textsc{k}$B$\alpha$ degradation to block expression of the NF-$textsc{k}$B-dependent genes in microglia when activated by $\beta$-amyloid peptide (A$\beta$). We also showed that when microglia is activated by $A\beta$42, the expression of A20 is suppressed. These findings place A20 in the category of ' protective ' genes, protecting cells from pro-inflammatory reper-toires induced in response to inflammatory stimuli in activated microglia via NF-$textsc{k}$B activation. In light of the gene and proteins for NF-$textsc{k}$B-dependent gene and inactivator for NF-$textsc{k}$B (I$textsc{k}$B$\alpha$), the observations now reported suggest that UDCA plays a role in supporting the attenuation of the production of pro-inflammatory cytokines and NO via inactivation of NF-$textsc{k}$B. Moreover, an NF-$textsc{k}$B inhibitor such as A20 can collaborate and at least enhance the anti-inflammatory effect in microglia, thus giving a potent benefit for the treatment of neurodegenerative diseases such as AD.uch as AD.

• 생약학회지
• /
• 제49권2호
• /
• pp.182-187
• /
• 2018

Many plant derived phytochemicals have been considered as the main therapeutic strategy against Alzheimer's disease (AD). AD is a progressive neurodegenerative disorder, and the most predominant cause of dementia in the elderly. Cholinergic deficit, senile plaque/${\beta}$-amyloid ($A{\beta}$) peptide deposition and oxidative stress have been identified as three main pathogenic pathways which contribute to the progression of AD. We screened many different plant species for their effective use in both modern and traditional system of medicines. In this study, we tested that MeOH extract of the stem bark of Hopea chinensis (Merr.) Hand.-Mazz. (HCM) affects on the processing of Amyloid precursor portein (APP) from the APPswe over-expressing Neuro2a cell line. We showed that HCM reduced the secretion level of $A{\beta}42$ and $A{\beta}40$ in a dose dependent manner. We found that HCM increased over 1.5 folds of the secretion level of $sAPP{\alpha}$, a metabolite of ${\alpha}$-secretase. Furthermore, we found that HCM inhibited acetylcholinesterase activity in vitro. We suggest that the stem bark of Hopea chinensis may be a useful source to develop a therapeutics for AD.

• Annals of Rehabilitation Medicine
• /
• 제42권6호
• /
• pp.814-821
• /
• 2018

Objective To suggest rotation angles of fluoroscopy that can bypass the carotid sheath according to vertebral levels for cervical transforaminal epidural steroid injection (TFESI). Methods Patients who underwent cervical spine magnetic resonance imaging (MRI) from January 2009 to October 2017 were analyzed. In axial sections of cervical spine MRI, three angles to the vertical line (${\alpha}$, angle not to insult carotid sheath; ${\beta}$, angle for the conventional TFESI; ${\gamma}$, angle not to penetrate carotid artery) were measured. Results Alpha (${\alpha}$) angles tended to increase for upper cervical levels ($53.3^{\circ}$ in C6-7, $65.2^{\circ}$ in C5-6, $75.3^{\circ}$ in C4-5, $82.3^{\circ}$ in C3-4). Beta (${\beta}$) angles for conventional TFESI showed a constant value of $45^{\circ}$ to $47^{\circ}$ ($47.5^{\circ}$ in C6-7, $47.4^{\circ}$ in C5-6, $45.7^{\circ}$ in C4-5, $45.0^{\circ}$ in C3-4). Gamma (${\gamma}$) angles increased at higher cervical levels as did ${\alpha}$ angles ($25.2^{\circ}$ in C6-7, $33.6^{\circ}$ in C5-6, $43.0^{\circ}$ in C4-5, $56.2^{\circ}$ in C3-4). Conclusion The risk of causing injury by penetrating major vessels in the carotid sheath tends to increase at upper cervical levels. Therefore, prior to cervical TFESI, measuring the angle is necessary to avoid carotid vessels in the axial section of CT or MRI, thus contributing to a safer procedure.

• 한국미생물·생명공학회지
• /
• 제17권5호
• /
• pp.421-428
• /
• 1989

물성조정제로서 고전도의 다당류를 생산하는 토양세균을 분리 선발하여 동정한 결과 Pseudomonas delafieldii로 판단되었다. 이 세균의 증식을 위한 pH와 온도는 6.5 및 3$0^{\circ}C$이었다. 최대 비성장속도는 0.25 h$^{-1}$이며 염류배지에서 다당류의 비생산속도, 증식수율 및 다당류 생산수율은 6.25mg/g-cell/h, 54.5%, 38.39%이었다. 본 다당류는 포도당과 gluconolactone을 몰비로 1.9：1.0로 함유한 $\beta$-glucan 으로 추정되었으며 원인 분석결과 탄소 31.85% 수소 31.85% 수소 5.l5%를 함유하고 아세틸기를 1.35%가지고 있었으며 고유점도는 42.84dl/g, 분자량은 5.64$\times$$10^7$이었다.

• 한국학교보건학회지
• /
• 제18권2호
• /
• pp.63-71
• /
• 2005

Purpose： This study explores the relationship between the health behavior and knowledge of elementary school students and the socio-economic status of their parents. Methods： A self-administrated questionnaire was provided to 238 students and their parents living within Seoul metropolitan area. The questionnaires were administered during December 2003. The relationship betweensocio-economic status, health behavior (19 items) and health knowledge (15 items) were analyzed using correlation, t-test and one-way ANOVA. Multiple regression analyses were also performed in order to investigate the relationship between health behavior and socio-economic status. Results： The results of multiple regression demonstrated that the health behavior of children living in apartments was 2.29 higher ($\beta$=2.29) than those not living in apartments. Health behavior scores among students with highly educated mothers were 0.42 higher ($\beta$=0.42) than those for students with relatively uneducated mothers. These numbers represent statistically significant values. Conclusion： Since health behavior appears to be directly proportional to socio-economic status ofthe parent, active intervention may be required in order to improve the health behavior of children with poorly educated mothers and who live in independent or multi-household houses.

• BMB Reports
• /
• 제42권6호
• /
• pp.380-386
• /
• 2009

In neurodegenerative diseases, such as Alzheimer' and Parkinson', microglial cell activation is thought to contribute to CNS injury by producing neurotoxic compounds. Prothrombin and kringle-2 increase levels of NO and the mRNA expression of iNOS, IL-1$\beta$, and TNF-$\alpha$ in microglial cells. In contrast, the human prothrombin kringle-2 derived peptide NSA9 inhibits NO release and the production of pro-inflammatory cytokines such as IL-1$\beta$, TNF-$\alpha$, and IL-6 in LPS-activated EOC2 microglia. In this study, we investigated the anti-inflammatory effects of NSA9 in human prothrombin- and kringle-2-stimulated EOC2 microglia. Treatment with 20-100 ${\mu}M$ of NSA9 attenuated both prothrombin- and kringle-2-induced microglial activation. NO production induced by MAPKs and NF-$\kappa$B was similarly reduced by inhibitors of ERK (PD98059), p38 (SB203580), NF-$\kappa$B (N-acetylcysteine), and NSA9. These results suggest that NSA9 acts independently as an inhibitor of microglial activation and that its effects in EOC2 microglia are not influenced by the presence of kringle-2.