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Optimization of Medium for Lipase Production from Zygosaccharomyces mellis SG1.2 Using Statistical Experiment Design

  • Pramitasari, Marisa Dian;Ilmi, Miftahul
    • Microbiology and Biotechnology Letters
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    • v.49 no.3
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    • pp.337-345
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    • 2021
  • Lipase (triacylglycerol lipase, EC 3.1.1.3) is an enzyme capable of hydrolyzing triacylglycerol, to produce fatty acids and glycerol and reverse the reaction of triacylglycerol synthesis from fatty acids and glycerol through transesterification. Applications of lipase are quite widespread in the industrial sector, including in the detergent, paper, dairy, and food industries, as well as for biodiesel synthesis. Lipases by yeasts have attracted industrial attention because of their fast production times and high stability. In a previous study, a lipase-producing yeast isolate was identified as Zygosaccharomyces mellis SG1.2 and had a productivity of 24.56 U/mg of biomass. This productivity value has the potential to be a new source of lipase, besides Yarrowia lypolitica which has been known as a lipase producer with a productivity of 0.758 U/mg. Lipase production by Z. mellis SG1.2 needs to be increased by optimizing the production medium. The aims of this study were to determine the significant component of the medium for lipase production and methods to increase lipase production using the optimum medium. The two methods used for the statistical optimization of production medium were Taguchi and RSM (Response Surface Methodology). The data obtained were analyzed using Minitab 18 and SPSS 23 software. The most significant factors which affected lipase productivity were olive oil and peptones. The optimum medium composition consisted of 1.02% olive oil, 2.19% peptone, 0.05% MgSO4·7H2O, 0.05% KCl, and 0.2% K2HPO4. The optimum medium was able to increase the lipase productivity of Z. mellis SG1.2 to 1.8-fold times the productivity before optimization.

Analysis of Important Factors for Sustainable Fishing Village Development (지속가능한 어촌마을 개발을 위한 중요 요소 분석)

  • Lee, Chan;Park, Ji-Hoon;Kang, Hyun-Kyung
    • Journal of Korean Society of Rural Planning
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    • v.26 no.1
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    • pp.23-38
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    • 2020
  • This study is aimed at the deduction of the important priority factors by setting the comprehensive rate in each of the economy, sociability and environmental areas for the sustainable development of fishing villages. As the research methods, review of domestic and overseas literature and Delphi questionnaire survey on experts, and hierarchical structure analysis were executed. Accordingly, this study is aimed at providing the basic data at the time of establishing plans by assessing the level of importance and priorities for each item through the deduction of the elements necessary for the fishing village plan. Key results of the study are as follows. First, as the results of review of domestic and overseas literature related to sustainability indices of fishing villages, a total of 50 items in the areas of economy, sociability and environment were deduced. Second, validity for each area was confirmed through Delphi questionnaire survey on experts. Third, as the results of factor analysis conducted on the basis of the hierarchical structure analysis for detailed items for each sector, 3 factors in the economic area, 2 factors in the social area and 3 factors in environment area were deduced. Fourth, as the results of the relative importance of the higher tiers, economic sector (0.528) displayed results that are more than 2 times higher than the social (0.261) and environmental (0.212) sectors. Establishment of foundation for independent competition (0.105), balanced development of small fishing village (0.086) and population engaged in economic activities in comparison to the fishing village population (0.077) were analyzed as the most important detailed items. Based on these results, experts pointed out that the economic sector is most important for the sustainable development of fishing village and, in detail, priority must be placed on the balanced development on the basis if the extent of independence of the fishing villages, and emphasized activation of residents engaged in economic activities in fishing villages in order to support them. That is, in order to increase the level of independence of the fishing villages, it was determined that there is a dire needs for expansion of diversified specialized businesses and infrastructural facilities through agreement by the residents capable of economic activities, and means of activating fishing villages through securing of budget at the local autonomous government level to provide support for the residents.

A Case Study on Villagers' Participation and Project Achievement in Rural Development - Focusing on Saemaul Undong Project in Myanmar - (농촌지역개발의 주민참여와 사업성과에 관한 사례 연구 - 미얀마 농촌공동체 개발사업을 중심으로 -)

  • Lee, Seung-won;Shim, Sung-hee
    • Journal of Korean Society of Rural Planning
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    • v.26 no.3
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    • pp.79-89
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    • 2020
  • Since the Republic of Korea had been registered as the 24th member of OECD DAC in 2009, recent trend of Korea's ODA has showed drastic increase including agriculture and rural development sector. Particularly, various rural development projects have been implemented adopting development experience and methodology of Saemaul Undong (SMU, New Village Movement). This study implemented statistical analysis between villagers' participation and achievement of rural community development project, based on practical data out of 'Saemaul Undong Project in Myanmar', to suggest meaningful implication in terms of participatory rural development. It emphasized the importance and necessity of villagers' participation in rural development through regression analysis that proved positive correlation between villagers' participation and rural development project. It proves that income-generation part has significantly greater influence than capacity-building and living environment part in terms of rural community development project: Based on the analysis, comparing impact of each independent variable, income generation has 1.88 and 1.68 times greater impact than capacity-building and living environment respectively. The result, on the other side, rather raise the importance of careful consideration for project design and implementation to harmonize those three parts altogether especially rural development for developing countries: capacity-building and living environment parts suggests essential foundation to make income generation successful that enables to secure project achievement and sustainability.

The Impact of Job Characteristics and Value Congruence on Employee Retention: An Empirical Study from Lebanon

  • AL SHAHER, Shaher;ZREIK, Mohamad
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.3
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    • pp.171-180
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    • 2022
  • Employee retention is becoming a crucial idea for businesses that aim to improve their performance, resulting in an increasing emphasis on the topic in today's society. Employee retention refers to an organization's ability to retain its employees. The retention of workers may be viewed as a technique used by companies to retain their personnel since it is linked to employer efforts to maintain their staff. Online surveys were initially done between April and July 2021. Furthermore, the study's primary focus was employees of small and medium enterprises. To collect data, the questionnaires were distributed through Google forms. The survey used a snowballing technique because the questionnaires were circulated among the participants. Based on the results, the research found that Perceived Organizational Support (POS) and Perceived Organizational Justice (POJ) mediate the relationship of Value Congruence (VC) and Job Characteristics (JS) and employee retention in the Lebanese Retail Sector. Employees appreciate POS because it satisfies their desires for acceptance, esteem, and connection and offers comfort during times of stress. Employers must give priority to equal effort to ensure equitable treatment for workers at work. By integrating justice and equality in all departments, the performance of workers may be enhanced.

An IoT-Aware System for Managing Patients' Waiting Time Using Bluetooth Low-Energy Technology

  • Reham Alabduljabbar
    • International Journal of Computer Science & Network Security
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    • v.24 no.3
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    • pp.83-92
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    • 2024
  • It is a common observation that whenever any patient arrives at the front desk of a hospital, outpatient clinic, or other health-associated centers, they have to first queue up in a line and wait to fill in their registration form to get admitted. The long waiting time without any status updates is the most common complaint, worrying health officials. In this paper, UrNext, a location-aware mobile-based solution using Bluetooth low-energy (BLE) technology, is presented to solve the problem. Recently, a technology-oriented method has been gaining popularity in solving the healthcare sector's problems, namely the Internet of Things (IoT). The implementation of this solution could be explained through a simple example that when a patient arrives at a clinic for her consultation. There, instead of having to wait in long lines, she will be greeted automatically, receive a push notification telling her that she has been admitted along with an estimated waiting time for her consultation session. This will not only provide the patients with a sense of freedom but would also reduce uncertainty levels that are generally observed, thus saving both time and money. This work aimed to improve clinics' quality of services and organize queues and minimize waiting times in clinics, leading to patient comfortability and reducing the burden on nurses and receptionists. The results demonstrated that the presented system was successful in its performance and helped achieve high usability.

A study on the site analysis considering a fused relationship of the architectural site and surrounding context (건축대지와 주변 컨텍스트의 융합적인 연계성을 고려한 대지분석에 관한 연구)

  • Jun, In-Mok
    • Journal of the Korea Convergence Society
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    • v.8 no.5
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    • pp.275-285
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    • 2017
  • The architectural site exists in the context of the surrounding environment as the background of architectural design, and the architecture exists in the connection between the site and the surrounding environment therefore, the plan of the building starts from the analysis of the site, and its consequences too meaning can be found in the relation with the surrounding environment. Ironically, however, there are no direct papers describing site analysis with this meaning. Therefore, this paper categorizes the site analysis items around the four environments of Land, Water, Fire, and Wind that constitute the environment of the ecosystem, furthermore, in order to apply the result of the fusion information to the architectural design by synthesizing the organic correlation between the inside and the surrounding environment, i created the '9sectors system', which is a combination of 'horizontal 3 and vertical 3' to provide a more rational and efficient architectural plan. Through the progress of the project using this system, it is considered that it is a more applicable method of the site analysis data than the existing site analysis method.

The Development of N2O Emission Factor at Killn Type Pyrolysis Melting Facility (외열킬른형 열분해용융시설의 N2O 배출계수 개발)

  • Yun, Hyun-Ki;Lee, Dae Kyeom;Cho, Changsang;Kang, Seongmin;Yoon, Young joong;Jeon, Youngjae;Jeon, Eui Chan
    • Journal of Climate Change Research
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    • v.8 no.3
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    • pp.231-237
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    • 2017
  • In this study, the $N_2O$ emission factor of the facility was developed by measuring the kiln type pyrolysis melting facility. This used PAS (Photoacoustic Spectroscopy) method and measured the $N_2O$ emission concentration. From March 2016 to April 2016, it was measured over a total of two times and $N_2O$ concentrations were measured continuously for 24 hours using a 24 hour continuous measuring instrument (LSE-4405). The measured $N_2O$ emission concentration of the pyrolysis melting facility was 0.263 ppm on average and the emission concentration distribution in the range of 0.013~0.733 ppm was obtained. Therefore, the $N_2O$ emission factor of the kiln-type pyrolysis melting facility was estimated to be $0.829gN_2O/ton$-Waste. As a result of comparing the $N_2O$ emission factor of the thermal kiln type pyrolysis melting facility and the previous study, previous studies were about 18 times higher. It is estimated that this is due to the difference of furnace temperature, oxygen concentration and denitrification facilities. It is considered that the study of the emission factor of pyrolysis melting facility is an important factor in improving the credibility of greenhouse gas inventory in waste incineration sector.

Present Status of Fisheries Wealth in Angola

  • Konda, Fredy Ditomene Mbala
    • Journal of Marine Bioscience and Biotechnology
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    • v.3 no.1
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    • pp.1-6
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    • 2008
  • Angola has a coastline of about 1,650 km long. Two diverging current namely, the Angola current with its warm water from the north and the cold Benguella Current in the south create a strong up-welling with a high productive ecosystem for marine resources. The area from Lobito to the mouth of the Cunene River, also known as the Southern fishing zone is by far the most productive of Angola's fishing zones. In 1977, the total potential of its marine fisheries sector was estimated at more than 700,000 tonnes per annum. In 2003, the Total Allowable Catch (TAC) established for demersal species was 57,600 tonnes and 160,000 tonnes for pelagic species. The most important resources are various marine demersal and pelagic fish including pilchard and the Cape and Cunene horse Mackerel (Tranchurus capensis and T. trecae). Sardinellas (Sardinella aurita and S. maderensis) are fished in parallel with horse mackerel. The rest of the catches are mainly demersal spp. and some deep water crustaceans. The demersal sppecies consist of Hake (Merluccius polli and M. capensis) and the large eye dentex spp. Tunas are caught at certain times of the year whilst some marine shrimp are also harvested from the Angolan waters. Angola also has several high value freshwater fish species, exploited by about 255 fishers. Tilapia sp. is among the most important and abundant fresh water fish found in Angola. Other species include the catfish (Clarias gariepinus) and fresh water prawns (Macrobrachuin rosenbergii). Some aquaculture ponds have been established in the country side, but due to lack of investment, proper training and the impact of civil war have seriously limited developments in the sub sector.

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Analysis on Relationships of Migratory Birds Species·Population due to Water-Ecosystem : Shinan-gun Benangkimi wetland (수생태에 따른 철새의 종·개체수 관계 분석 : 신안군 배낭기미습지)

  • Kim, Dong Hyun;Lee, Seung Oh
    • Journal of Korean Society of Disaster and Security
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    • v.11 no.1
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    • pp.7-14
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    • 2018
  • The Benangkimi wetland, which serves as a stopover place for migratory birds in the Dadohae Marine National Park in Wando-gun, Jeollanam-do, is a place where various species and a large number of migratory birds are observed. In order to provide an effective and scientific management plan for the habitat environment of migratory birds, we carried out field survey of Benangkimi wetland. The field survey and survey on habitat use of migratory birds were conducted in parallel to obtain the basic data of hydraulics and hydrology. The hydraulic and hydrological survey of Benangkimi wetland was conducted 1-2 times a year during the period of 2015-2016 (two years). It was classified into 4 sectors according to the topography and geology, rainfall, hydraulic characteristics, and wetland conditions. The 3 sectors same as the migratory birds survey sector and the 1 sector as the inflow of the wetland were analyzed. The survey also focused on small migratory birds arriving at Benangkimi wetland during the spring and autumn season. As a result of investigating the population and species in each section, the habitat environment was different according to the water depth, sediment thickness and salinity. Migratory birds prefer hydraulic and hydrological characteristics. This study will be used for the scientific management of Benangkimi wetland, which serves as a stopping point for migratory birds, and it will contribute to basic data of ecology - hydraulics by examining the relationship between habitat environment and hydrological data.

Vulnerability Assessment of Human Health Sector due to Climate Change: Focus on Ozone (기후변화에 따른 보건 분야의 취약성 평가: O3을 중심으로)

  • Lee, Jae-Bum;Lee, Hyun-Ju;Moon, Kyung-Jung;Hong, Sung-Chul;Kim, Deok-Rae;Song, Chang-Keun;Hong, You-Deog
    • Journal of Korean Society for Atmospheric Environment
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    • v.28 no.1
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    • pp.22-38
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    • 2012
  • Adaptation of climate change is necessary to avoid unexpected impacts of climate change caused by human activities. Vulnerability refers to the degree to which system cannot cope with impacts of climate change, encompassing physical, social and economic aspects. Therefore the quantification of climate change impacts and its vulnerability is needed to identify vulnerable regions and to setup the proper strategies for adaptation. In this study, climate change vulnerability is defined as a function of climate exposure, sensitivity, and adaptive capacity. Also, we identified regions vulnerable to ozone due to climate change in Korea using developed proxy variables of vulnerability of regional level. 18 proxy variables are selected through delphi survey to assess vulnerability over human health sector for ozone concentration change due to climate change. Also, we estimate the weighting score of proxy variables from delphi survey. The results showed that the local regions with higher vulnerability index in the sector of human health are Seoul and Daegu, whereas regions with lower one are Jeollanam-do, Gyeonggi-do, Gwangju, Busan, Daejeon, and Gangwon-do. The regions of high level vulnerability are mainly caused by their high ozone exposure. We also assessed future vulnerability according to the Intergovernmental Panel on Climate Change (IPCC) Special Report on Emissions Scenarios (SRES) A2, A1FI, A1T, A1B, B2, and B1 scenarios in 2020s, 2050s and 2100s. The results showed that vulnerability increased in all scenarios due to increased ozone concentrations. Especially vulnerability index is increased by approximately 2 times in A1FI scenarios in the 2020s. This study could support regionally adjusted adaptation polices and the quantitative background of policy priority as providing the information on the regional vulnerability of ozone due to climate change in Korea.