Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Optimization of Medium for Lipase Production from Zygosaccharomyces mellis SG1.2 Using Statistical Experiment Design

  • Received : 2021.06.29
  • Accepted : 2021.07.23
  • Published : 2021.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Lipase (triacylglycerol lipase, EC 3.1.1.3) is an enzyme capable of hydrolyzing triacylglycerol, to produce fatty acids and glycerol and reverse the reaction of triacylglycerol synthesis from fatty acids and glycerol through transesterification. Applications of lipase are quite widespread in the industrial sector, including in the detergent, paper, dairy, and food industries, as well as for biodiesel synthesis. Lipases by yeasts have attracted industrial attention because of their fast production times and high stability. In a previous study, a lipase-producing yeast isolate was identified as Zygosaccharomyces mellis SG1.2 and had a productivity of 24.56 U/mg of biomass. This productivity value has the potential to be a new source of lipase, besides Yarrowia lypolitica which has been known as a lipase producer with a productivity of 0.758 U/mg. Lipase production by Z. mellis SG1.2 needs to be increased by optimizing the production medium. The aims of this study were to determine the significant component of the medium for lipase production and methods to increase lipase production using the optimum medium. The two methods used for the statistical optimization of production medium were Taguchi and RSM (Response Surface Methodology). The data obtained were analyzed using Minitab 18 and SPSS 23 software. The most significant factors which affected lipase productivity were olive oil and peptones. The optimum medium composition consisted of 1.02% olive oil, 2.19% peptone, 0.05% MgSO4·7H2O, 0.05% KCl, and 0.2% K2HPO4. The optimum medium was able to increase the lipase productivity of Z. mellis SG1.2 to 1.8-fold times the productivity before optimization.

Keywords

Acknowledgement

The experiment of this study was funded by RTA Grant year 2020 (2488/UN1.P.III/DIT-LIT/PT/2020), and the publication was funded by RTA Grant year 2021 (3143/UN1.P.III/DIT-LIT/PT/2021). Both grants were from the Universitas Gadjah Mada, Indonesia.

References

  1. Rekha KSS, Lakshmi MVVC, Devi VS, Kumar MS. 2012. Production and optimization of lipase from Candida rugosa using groundnut oilcake under solid state fermentation. Int. J. Res. Eng. Technol. 1: 571-577. https://doi.org/10.15623/ijret.2012.0104008
  2. Alami NH, Nasihah L, Umar RLA, Kuswytasari ND, Zulaika E, Shovitri M. 2017. Lipase production in lipolytic yeast from Wonorejo mangrove area. AIP Conf. Proceed. 1854: 1-11.
  3. Fan X, Niehus X, Sandoval G. 2012. Lipases as biocatalyst for biodiesel production. Method. Mol. Biol. 861: 471-483. https://doi.org/10.1007/978-1-61779-600-5_27
  4. Ugur A, Sarac N, Boran R, Ayaz B, Ceylan O, Okmen G. 2014. New lipase for biodiesel production: partial purification and characterization of LipSB 25-4. ISRN Biochem. 2014: 1-8.
  5. Fjerbaek LKV, Christensen, Norddahl B. 2009. A review of the current state of biodiesel production using enzymatic transesterification. Biotechnol. Bioeng. 102: 1298-1315. https://doi.org/10.1002/bit.22256
  6. Lanka S, Latha JNL. 2015. Taguchi design of experiments for the optimization of lipase production by Emericella nidulans DAOM 222012 isolated from palm oil mill effluent (pome) dump sites. Int. J. Appl. Bio. Pharm. Technol. 6: 64-72.
  7. Thakur S. 2012. Lipases, its sources, properties and applications: a review. Int. J. Sci. Eng. Res. 3: 1-29.
  8. Galvagno MA, Iannone LJ, Bianchi J, Kronberg F, Rost E, Carstens MR, et al. 2011. Optimization of biomass production of mutant of Yarrowia lypolitica with an increased lipase activity using raw glycerol. Rev. Argentina de Microbiol 43: 218-225. https://doi.org/10.1590/S0325-75412011000300010
  9. Oliveira F, Souza CE, Peclat VROL, Salgado JM, Ribeiro BD, Coelho MAZ, et al. 2017. Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reaction. Food Bioprod. Process 2017: 1-39.
  10. Azeredo LAI, Gomes PM, SantOAnna GL, Castilho LR, Freire DMG. 2007. Production and regulation of lipase activity from Penicillium restrictum in submerged and solid-state fermentations. Curr. Microbiol. 54: 361-365. https://doi.org/10.1007/s00284-006-0425-7
  11. Palilu PT, Kasiamdari RS, Ilmi M. 2019. Potential yeast from Indonesian wild forest honey showing ability to produce lipase for lipid transesterification. Microbiol. Biotechnol. Lett. 47: 555-564. https://doi.org/10.4014/mbl.1907.07008
  12. Corzo G, Revah S. 1999. Production and characteristics of the lipase from Yarrowia lipolytica 681. Bioresour. Technol. 70: 173-180. https://doi.org/10.1016/S0960-8524(99)00024-3
  13. Bhosale HJ, Uzma SZ, Bismile PC. 2015. Optimization of lipase production by thermo-alkalophilic Bacillus sp. 8C. Res. J. Microbiol. 10: 523-531. https://doi.org/10.3923/jm.2015.523.532
  14. Colla LM, Primaz AL, Benedetti S, Loss RA, de Lima M, Reinehr CO, et al. 2016. Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi. Braz. J. Microbiol. 47: 461-467. https://doi.org/10.1016/j.bjm.2016.01.028
  15. Heravi MK, Eftekhar F, Yakhchali B, Tabandeh F. 2008. Isolation and identification of a lipase producing Bacillus sp. from soil. Pakistan J. Biol. Sci. 11: 740-745. https://doi.org/10.3923/pjbs.2008.740.745
  16. Burkert JFM, Maugeri F, Rodrigues MI. 2004. Optimization of extracellular lipase production by Geotrichum sp. using factorial design. Bioresour. Technol. 91: 77-84. https://doi.org/10.1016/S0960-8524(03)00152-4
  17. Kwon DY, Rhee JS. 1986. A simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J. Am. Oil Chem. Soc. 63: 89-92. https://doi.org/10.1007/BF02676129
  18. Shukla BN, Desai PV. 2016. Isolation, characterization and optimization of lipase producing Pseudomonas spp. from oil contaminated sites. Int. J. Curr. Microbiol. Appl. Sci. 5: 902-909. https://doi.org/10.20546/ijcmas.2016.505.093
  19. Adham NZ, Ahmed EM. 2009. Extracellular lipase of Aspergillus niger NRRL3: production, partial purification and properties. Indian J. Microbiol. 49: 77-83. https://doi.org/10.1007/s12088-009-0004-2
  20. Tan T, Zhang M, Xu J, Zhang J. 2004 Optimization of culture conditions and properties of lipase from Penicillium camembertii Thom PG-3. Process Biochem. 39: 1495-1502. https://doi.org/10.1016/S0032-9592(03)00296-6
  21. Soleymani S, Alizadeh H, Mohammadian H, Rabbani E, Moazen F, Sadeghi HMM, et al. 2017. Efficient media for high lipase production: one variable at a time approach. Avicenna J. Med. Biotechnol. 9: 82-86.
  22. Kumar DJM, Rajan R, Priyadarshini S, Lawrence L, Sandhiyachittybabu, Kalaichelvan PT. 2012. Characterization of lipase and protease from Serratia marcescens DEPTK21 and its destaining capability. Asian J. Exp. Biol. Sci. 3: 621-628.
  23. Trismilah P, Sarnianto, Wahjono E. 2016. Optimization of lipase production by Bacillus licheniformis F11.4 using response surface methodology. Microbiol. Indonesia 10: 139-148. https://doi.org/10.5454/mi.10.4.4
  24. Alam MZ, Elgharbawy AA, Salleh HM. 2013. Optimization and characterization of Candida cylindracea lipase produced from palm kernel cake by solid-state bioconversion. AIChE Ann. Meet. 2013: 1-7.
  25. Keshani S, Chuah AL, Nourouzi MM, Russly AR, Jamilah B. 2010. Optimization of concentration process on pomelo fruit juice using response surface methodology (RSM). Int. Food Res. J. 17: 733-742.
  26. Nurmiah S, Syarief R, Sukarno, Peranginangin R, Nurtama B. 2013. Application of response surface methodology in the optimization of process conditions of alkali treated cottonii (ATC) processing. JPB Kelautan dan Perikanan 8: 9-22.
  27. Kumari KS, Babu IS, Rao GH. 2008. Process optimization for citric acid production from raw glycerol using response surface methodology. Indian J. Biotech. 1: 496-501.
  28. Raissi S, Farzani RE. 2009. Statistical process optimization through multi-response surface methodology. World Acad. Sci. Eng. Technol. 1: 267-271.
  29. Prabudi M, Nurtama B, Purnomo EH. 2018. Aplication of response surface methodology (RSM) using historical data on optimation burger production process. J. Mutu Pangan 5: 109-115.
  30. Noordin MY, Venkatesh VC, Sharif S, Elting S, Abdullah A. 2004. Application of response surface methodology in describing the performance of coated carbide tools when turning AISI 1045 steel. J. Mater. Process Technol. 145: 46-58. https://doi.org/10.1016/S0924-0136(03)00861-6
  31. Kathiravan T, Marykala J, Sundaramanickam A, Kumaresan S, Balasubramanian T. 2012. Studies on nutritional requirements of Pseudomonas aeruginosa for lipase production. Adv. Appl. Sci. Res. 3: 591-598.
  32. Musa H, Kasim FH, Gunny AAN, Gonipath SCB, Ahmad MA. 2019. Enhanced halophilic lipase secretion by Marinobacter litoralis SW-45 and its potential fatty acid esters release. J. Basic Microbiol. 59: 87-100. https://doi.org/10.1002/jobm.201800382
  33. Nwachukwu E, Ejike EN, Ejike BU, Onyeanula EO, Chikezie-Abba RO, Okorocha NA, et al. 2017. Characterization and optimization of lipase production from soil microorganism (Serratia marcescens). Int. J. Curr. Microbiol. App. Sci. 6: 1215-1231.
  34. Salwoom L, Rahman RNZRA, Salleh AB, Shariff FM, Convey P, Pearce D, et al. 2019. Isolation, characterisation, and lipase production of a cold-adapted bacterial strain Pseudomonas sp. LSK25 isolated from Signy Island, Antarctica. Molecules 24: 715. https://doi.org/10.3390/molecules24040715
  35. Rahman R, Masomian M, Salleh A, Basri M. 2009. A new thermostable lipase by Aneurinibacillus thermoaerophilus strain HZ: nutritional studies. Ann. Microbiol. 59: 133-139. https://doi.org/10.1007/BF03175610
  36. Kumar S, Kikon K, Upadhyay A, Kanwar SS, Gupta R. 2005. Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expr. Purif. 41: 38-44. https://doi.org/10.1016/j.pep.2004.12.010
  37. Freire DM, Teles EMF, Bon EPS, Anna GLS. 1997. Lipase production by Penicillium restrictum in a bench-scale fermenter effect of carbon and nitrogen nutrition, agitation, and aeration. Appl. Biochem. Biotechnol. 63-65: 409-421. https://doi.org/10.1007/BF02920442
  38. Mates A, Sudakevitz D. 1973. Production of lipase by Staphylococcus aureus under various growth conditions. J. Appl. Bacteriol. 36: 219-226. https://doi.org/10.1111/j.1365-2672.1973.tb04094.x