• 한국해양환경ㆍ에너지학회지
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• 제19권2호
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• pp.87-98
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• 2016

2009년 7월 3일부터 7월 27일까지 러시아 조사선 R/V Lavrentyev를 이용하여 러시아 연안으로부터 4개의 Line(D, R, E, A)을 따라 표층 30 m 수심의 시료를 26개의 정점(울릉분지와 일본분지를 포함한 동해의 전 수역)에서 채수하여 영양염 및 엽록소-${\alpha}$의 농도를 분석하였다. 냉수역보다 난수역에서 1.4배 높은 질산염을 제외한 나머지 항목들은 모두 난수역보다 냉수역에서 높게 나타났다($NH_4$, $PO_4$ 각 1.8배, $Si(OH)_4$ 1.2배, 엽록소-${\alpha}$ 1.9배). 암모니아와 인산염, 엽록소-${\alpha}$의 수평분포는 매우 유사한 분포를 보이며, 한류와 저층수의 용승 영향권에 있는 러시아 근해에서 최대치를 보이고, 울릉분지에서 비교적 낮은 분포를 보인다. 반면, 질산염은 대마난류수의 직접적인 영향권에 있는 울릉분지에서 최대치를 보이며, 점차 북상할수록 감소한다. N/P 비는 한류수계보다 대마난류 중층수에서 가장 높은 값을 보이며, 대마난류수는 동해로 유입되는 질산염의 주요 공급원으로 작용하고 있다. 그러나 난수역에서 인산염의 평균 농도는 $0.2{\mu}M$ 이하로 식물플랑크톤 성장의 제한 요인으로 작용할 수 있는 반면, 냉수역에서의 높은 농도는 엽록소-${\alpha}$와 직접적인 상관성을 보이고 있다. 해양환경에 영향을 미치는 주요인을 분석하기 위한 주성분 분석결과 주성분 I은 수온에 의해 동해의 해양환경이 주로 영향을 받으며, 주성분 II는 영양염과 엽록소-${\alpha}$ 가 주요인으로 작용한다. 따라서 연구해역의 해양환경은 수온에 지배되며, 그에 따라 냉수역과 온수역으로 구분되는 특성을 보였다.

• 한국수정란이식학회지
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• 제21권3호
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• pp.191-198
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• 2006

본 연구는 $2001{\sim}2005$년(5년간) 제주도에서 사육중인 제주 한우와 흑우를 체내 수정란의 생산과 수정란 이식 기술을 통하여 조기 증식하고자 실시하였다. 한우 고등 등록우 286두와 흑우 경산우 69두(총 355두)에 대하여 발정 주기에 관계없이 CIDR를 질내에 삽입 후 7일째부터 성선 자극 호르몬($Folltropin^{(R)}-V$) 400 mg을 50 mg씩 균등하게 나누어 4일간 12시간 간격으로 근육주사하였다. 투여 6회째에 CIDR를 제거하였으며, 동시에 $PGF_{2{\alpha}}$ 25mg을 근육 주사하여 과배란을 유기하였다. 공란우의 인공수정은 $PGF_{2{\alpha}}$투여 후 발정을 확인하고 12 시간 간격으로 3회 인공 수정하였으며, 2회 인공 수정시 GnRH $250{\mu}g$을 근육 주사하였다. 수정란 회수는 1차 인공 수정 후 $7{\sim}8$일째에 비외과적 방법으로 채란하였다. 수란우는 CIDR를 7일간 삽입하였다가 제거하는 동시에 $PGF_{2{\alpha}}$ 25 mg을 근육 주사하여 공란우와 발정을 동기화 시켰다. 수정란 이식은 발정이 동기화된 수란우 1,219두에 대하여 6명의 수정란 이식 시술자가 비외과적 방법으로 황체가 존재하는 자궁각에 이식하였다. 한우 및 흑우 공란우의 평균 회수 총 난자수 및 이식 가능 수정란 수는 각각 7.4개 및 4.7개 였으며, 회수 총 난자수는 2 품종간에 차이가 없었으나, 이식 가능 수정란 수는 한우(5.0개)가 흑우(3.5개)에 비해 많았다(p<0.05). 공란우의 반복 과배란 처리에 따라 흑우에서는 과배란처리 두수 대비 수정란 채란우 비율이 감소되었으며(p<0.05), 한우에서는 회수 총 난자수 및 이식 가능 수정란 수의 감소가 나타났다(p<0.05). 과배란 처리 계절에 따른 수정란 회수 성적은 여름(5.6개)이 겨울(2.9개)에 비해 이식 가능 수정란의 수가 많았다(p<0.01). 수정란 이식 후 수태율은 평균 40%를 나타내었으며, 공란우의 품종간 차이가 없었으며, 이식년도가 2001년에서 2004년으로 경과될 때 수태율이 증가하였으며(p<0.05), 수정란 이식 시술자에 따른 수태율의 차이가($33.0{\sim}45.9%$) 인정되었다(p<0.01). 본 연구의 결과는 제주 한우 및 흑우에서 CIDR를 이용한 과배란 처리 및 수란우의 발정동기화 처리는 한우 체내 수정란의 안정적인 생산과 수란우의 준비가 가능함을 보여 주었다.

• Archives of Pharmacal Research
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• 제26권4호
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• pp.275-278
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• 2003

A new acylglycosyl Sterol (4) was isolated from the MeOH extract of Quisqualis Fructus together with four known compounds. On the basis of spectroscopic data, their structures were elucidated as clerosterol (1), betulinic acid (2), methylursolate (3), 3-Ο-[6 -Ο-(8Z-octadecenoyl)-$\beta$-D-glucopyranosyl]-clerosterol (4) and $\alpha$-xylofuranosyluracil (5).

• 한국수정란이식학회지
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• 제32권3호
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• pp.87-94
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• 2017

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at $37^{\circ}C$. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

• 한국축산식품학회지
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• 제39권5호
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• pp.725-741
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• 2019

In the current study, we first investigated a method for directly transforming lactose into galacto-oligosaccharides (GOS) for manufacturing low-lactose and GOS-enriched skim milk (GSM) and then evaluated its prebiotic potential by inoculating five strains of Bifidobacterium spp. In addition, fermented GSM (FGSM) was prepared using a potentially probiotic Lactobacillus strain and its fermentation characteristics and antioxidant capacities were determined. We found that GOS in GSM were metabolized by all five Bifidobacterium strains after incubation and promoted their growth. The levels of antioxidant activities including radical scavenging activities and 3-hydroxy-3-methylglutaryl-CoA reductase inhibition rate in GSM were significantly increased by fermentation with the probiotic Lactobacillus strain. Moreover, thirty-nine featured peptides in FGSM was detected. In particular, six peptides derived from ${\beta}$-casein, two peptides originated from ${\alpha}s_1$-casein and ${\kappa}$-casein were newly identified, respectively. Our findings indicate that GSM can potentially be used as a prebiotic substrate and FGSM can potentially prevent oxidative stress during the production of synbiotic fermented milk in the food industry.

• 대한한의학방제학회지
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• 제17권2호
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• pp.163-174
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• 2009

Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.

• 한국세라믹학회지
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• 제49권6호
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• pp.586-591
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• 2012

$Zr_2WP_2O_{12}$ powder, which has a negative thermal expansion coefficient, was synthesized by a solid-state reaction with $ZrO_2$, $WO_3$ and $NH_4H_2PO_4$ as the starting materials. The synthesis behavior was dependent on the solvent media used in the wet mixing process. The $Zr_2WP_2O_{12}$ powder prepared with a solvent consisting of D. I. water was fully crystallized at $1200^{\circ}C$, showing a sub-micron particle size. According to the results obtained from a thermal analysis, a $ZrP_2O_7$ was synthesized at a low temperature of $310^{\circ}C$, after which it was reacted with $WO_3$ at $1200^{\circ}C$. A new sintering additive, $Al(OH)_3$, was applied for the densification of the $Zr_2WP_2O_{12}$ powders. The cold isostatically pressed samples were densified with 1 wt% $Al(OH)_3$ additive or more at $1200^{\circ}C$ for 4 h. The main densification mechanism was liquid-phase sintering due to the liquid which resulted from the reaction with amorphous or unstable $Al_2O_3$ and $WO_3$. The densified $Zr_2WP_2O_{12}$ ceramics showed a relative density of 90% and a negative thermal expansion coefficient of $-3.4{\times}10^{-6}/^{\circ}C$. When using ${\alpha}-Al_2O_3$ as the sintering agent, densification was not observed at $1200^{\circ}C$.

• 한국축산식품학회지
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• 제34권3호
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• pp.362-371
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• 2014

This study utilized commercially available proteolytic enzymes to prepare egg-white protein hydrolysates (EPHs) with different degrees of hydrolysis. The antioxidant effect and functionalities of the resultant products were then investigated. Treatment with Neutrase yielded the most ${\alpha}$-amino groups (6.52 mg/mL). Alcalase, Flavourzyme, Protamex, and Ficin showed similar degrees of ${\alpha}$-amino group liberation (3.19-3.62 mg/mL). Neutrase treatment also resulted in the highest degree of hydrolysis (23.4%). Alcalase and Ficin treatment resulted in similar degrees of hydrolysis. All hydrolysates, except for the Flavourzyme hydrolysate, had greater radical scavenging activity than the control. The Neutrase hydrolysate showed the highest 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity ($IC_{50}=3.6mg/mL$). Therefore, Neutrase was identified as the optimal enzyme for hydrolyzing egg-white protein to yield antioxidant peptides. During Neutrase hydrolysis, the reaction rate was rapid over the first 4 h, and then subsequently declined. The $IC_{50}$ value was lowest after the first hour (2.99 mg/mL). The emulsifying activity index (EAI) of EPH treated with Neutrase decreased, as the pH decreased. The EPH foaming capacity was maximal at pH 3.6, and decreased at an alkaline pH. Digestion resulted in significantly higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ABTS radical scavenging activity. The active peptides released from egg-white protein showed antioxidative activities on ABTS and DHHP radical. Thus, this approach may be useful for the preparation of potent antioxidant products.

• 대한한의학회지
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• 제26권3호
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• pp.176-187
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• 2005

Objectives : This study was carried out to investigate the anti-inflammation, anti-development and curative effects of Oyaksunki-sangamibang (OSKM) on collagen-induced arthritis in Wistar rats and ICR mice. Materials & Methods : D experiment part II, the inhibitory effects of nitric oxide synthesis, pro-inflammatory cytokines, and cyclooxygenase were studied. In experiment part II, paw eduma volume and thickness of ankle joint were measured at 0, 10, 15, and 20 days after immunization. The incidence and arthritis score were evaluated 14 days after immunization, At 15 days after immunization, serum $TNF-\alpha$ was analyzed. In experiment part III paw edema volume and thickness of ankle joint were measured at 0, 10, and 15days after treatment. At 15 days after treatment, serum $TNF-\alpha$ was analyzed. Results : In experiment part I: 1. Nitric oxide synthesis ·md pro-inflammatory cytokines were inhibited significantly by OSKM extract. 2. Cyclooxygenase 2 (COX-2) was inhibited by OSKM extract. In experiment part II: Paw edema volume, thickness of ankle joint and serum $TNF-\alpha$ level of the teated group were significantly decreased compared with the control group at 20 days after immunization. In experiment part III: Incidence of arthritis was $70\%$. OSKM-treated group had no significant change on paw edema volume, thickness of ankle joint and serum $TNF-\alpha$ level. Conclusions : These results indicated that OSKM has anti-inflammation effects on the ICR mouse, and higher inhibitory effects on the onset but lower inhibitory effects on the progression of collagen-induced arthritis in rats.

• 한국전기전자재료학회논문지
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• 제25권2호
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• pp.85-90
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• 2012

In this paper, we compared and analyzed 3D silicon-oxide-nitride-oxide-silicon (SONOS) multi layer flash memory devices fabricated on nitride or oxide layer, respectively. The device fabricated on nitride layer has inferior electrical properties than that fabricated on oxide layer. However, the device on nitride layer has faster program / erase speed (P/E speed) than that on the oxide layer, although having inferior electrical performance. Afterwards, to find out the reason why the device on nitride has faster P/E speed, 1/f noise analysis of both devices is investigated. From gate bias dependance, both devices follow the mobility fluctuation model which results from the lattice scattering and defects in the channel layer. In addition, the device on nitride with better memory characteristics has higher normalized drain current noise power spectral density ($S_{ID}/I^2_D$>), which means that it has more traps and defects in the channel layer. The apparent hooge's noise parameter (${\alpha}_{app}$) to represent the grain boundary trap density and the height of grain boundary potential barrier is considered. The device on nitride has higher ${\alpha}_{app}$ values, which can be explained due to more grain boundary traps. Therefore, the reason why the devices on nitride and oxide have a different P/E speed can be explained due to the trapping/de-trapping of free carriers into more grain boundary trap sites in channel layer.