• Proceedings of the IEEK Conference
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• 1998.06a
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• pp.207-210
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• 1998

Brushless servomotor systems offer a great advantae in unmanned factories where a great number of servomotors are employed, because of its easy maintenance charateristics and controllability. This paper propose a sinusoidal type brushless DC motor speed servo system which has high efficiency and usability in industrial field as described above. And this servo system is realized by a new Auto-Tuning PI control algorithm and verified it's practical potential of implementation by self-designed driver system. In particular, DSP(digital signal processor) is adopted in this study as controller and sensor signal processor owing to their fast computational capability and suitable architecture.

• Bulletin of the Korean Chemical Society
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• v.33 no.1
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• pp.153-158
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• 2012

The hydrogenation of $AlB_2$-type BaGaGe exhibits a metal to insulator (MI) transition, inducing a puckering distortion of the original hexagonal [GaGe] layers. We investigate the electronic structure changes associated with the hydrogen-induced MI transition, using extended H$\ddot{u}$ckel tight-binding band calculations. The results indicate that hydrogen incorporation in the precursor BaGaGe is characterized by an antibonding interaction of $\pi$ on GaGe with hydrogen 1s and the second-order mixing of the singly occupied antibonding $\pi^*$ orbital into it, through Ga-H bond formation. As a result, the fully occupied bonding $\pi$ band in BaGaGe changes to a weakly dispersive band with Ge pz (lone pair) character in the hydride, which becomes located just below the Fermi level. The Ga-Ge bonds within a layered polyanion are slightly weakened by hydrogen incorporation. A rationale for this is given.

• Journal of Veterinary Clinics
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• v.15 no.1
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• pp.151-155
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• 1998

Abomasal motility was observed by needling to acupoints for 20 min. The acupoints used were guan yuan yurt da chang yurt dei yup pi yurt and hou hoi. The acupoint which showed the increase of the largest wave type was pi yu and the increase of wave type was observed 20 minutes after needling in hou hoi and dei yu acupoint. But there was no effect on abomasal motility after needling to guan yuan yu and da chang yu. On the other hands change in amplitude of the abomasal contraction after needling to these acupoints was not abserved. These results indicate that stimulation to pi yu acupoint would be the most useful to increase the abomasal motility.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.2
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• pp.261-267
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• 2013

The purpose of this study was to examine the psychometric structure of Sasang typology with four personality measures and examine the construct validity of Sasang Personality Questionnaire (SPQ). We measured biopsychological traits of 104 university students using SPQ, Temperament and Character Inventory (TCI), NEO-Personality Inventory (NEO-PI) and Myers-Briggs Type Indicator (MBTI). The construct validity of SPQ was analyzed with TCI, NEO-PI and MBTI using Pearson's correlation. The temperament features of each Sasang groups were compared with Analysis of Variance and Profile Analysis. The SPQ showed significant correlation with TCI Novelty-Seeking (r=.398), TCI Harm-Avoidance (r=-.333), TCI Reward-Dependence (r=.465), TCI Self-Directedness (r=0.340), NEO-PI Extraversion (r=0.489) and MBTI Extraversion-Introversion (r=-0.509). The SPQ, TCI and NEO-PI revealed significant differences between Sasang constitutional groups. We examined the temperament construct of the Sasang typology and the validity of SPQ with well established western biopsychosocial instruments. This study would contribute to the objective studies on the biopsychosocial mechanism of Sasang medicine.

• Journal of Life Science
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• v.24 no.4
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• pp.343-351
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• 2014

Phosphoinositide 3-kinase (PI3K) plays a central role in cell signaling and leads to cell proliferation, survival, motility, exocytosis, and cytoskeletal rearrangements, as well as specialized cell responses, superoxide production, and cardiac myocyte growth. PI3K is divided into three classes; type I PI3K is preferentially expressed in leukocytes and activated by ${\beta}{\gamma}$ subunits of heterotrimeric G-proteins. In this study, the cDNAs encoding the $PI3K{\gamma}$ gene were isolated from a brain cDNA library constructed using the flounder (Paralichthys olivaceus). The sequence of the isolated $PI3K{\gamma}$ was 1341 bp, encoding 447 amino acids. The nucleotide sequence of the $PI3K{\gamma}$ gene was analyzed with that of other species, including Oreochromis niloticus and Danio rerio, and it turned out to be well conserved during evolution. The $PI3K{\gamma}$ gene was subcloned into the expression vector pET-44a(+), and expressed in the E. coli BL21 (DE3) codon plus cell. The resulting protein was expressed as a fusion protein of approximately 49 kDa containing a C-terminal six-histidine extension that was derived from the expression vector. The expressed protein was purified to homogeneity by His-tag affinity chromatography and showed enzymatic activity corresponding to $PI3K{\gamma}$. The binding of wortmannin to $PI3K{\gamma}$, as detected by anti-wortmannin antisera, closely followed the inhibition of the kinase activities. The results obtained from this study will provide a wider base of knowledge on the primary structure and characterization of the $PI3K{\gamma}$ at the molecular level.

• BMB Reports
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• v.40 no.6
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• pp.845-852
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• 2007

The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 ($\underline{Ph}alaenopsis$ $\underline{PI}$ STILLATA # $\underline{15}$), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

• Molecules and Cells
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• v.22 no.1
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• pp.97-103
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• 2006

Phosphoinositides are critical regulators of ion channel and transporter activity. There are multiple isomers of biologically active phosphoinositides in the plasma membrane and the different lipid species are non-randomly distributed. However, the mechanism by which cells impose selectivity and directionality on lipid movements and so generate a non-random lipid distribution remains unclear. In the present study we investigated which structural elements of phosphoinositides are responsible for their subcellular location and movement. We incubated phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PI(4)P) and phosphatidylinositol 4,5-bisphosphate ($PI(4,5)P_2$) with short or long acyl chains in CHO and HEK cells. We show that phosphate number and acyl chain length determine cellular location and translocation movement. In CHO cells, $PI(4,5)P_2$ with a long acyl chain was released into the cytosol easily because of a low partition coefficient whereas long chain PI was released more slowly because of a high partition coefficient. In HEK cells, the cellular location and translocation movement of PI were similar to those of PI in CHO cells, whereas those of $PI(4,5)P_2$ were different; some mechanism restricted the translocation movement of $PI(4,5)P_2$, and this is in good agreement with the extremely low lateral diffusion of $PI(4,5)P_2$. In contrast to the dependence on the number of phosphates of the phospholipid head group of long acyl chain analogs, short acyl chain phospholipids easily undergo translocation movement regardless of cell type and number of phosphates in the lipid headgroup.

• International Journal of Control, Automation, and Systems
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• v.4 no.4
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• pp.438-447
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• 2006

Fuzzy controller's design depends mainly on the rule base and membership functions over the controller's input and output ranges. This paper presents two different approaches to deal with these design issues. A simple and efficient approach; namely, Fuzzy Subtractive Clustering is used to identify the rule base needed to realize Fuzzy PI and PD type controllers. This technique provides a mechanism to obtain the reduced rule set covering the whole input/output space as well as membership functions for each input variable. But it is found that some membership functions projected from different clusters have high degree of similarity. The number of membership functions of each input variable is then reduced using a similarity measure. In this paper, the fuzzy subtractive clustering approach is shown to reduce 49 rules to 8 rules and number of membership functions to 4 and 6 for input variables (error and change in error) maintaining almost the same level of performance. Simulation on a wide range of linear and nonlinear processes is carried out and results are compared with fuzzy PI and PD type controllers without clustering in terms of several performance measures such as peak overshoot, settling time, rise time, integral absolute error (IAE) and integral-of-time multiplied absolute error (ITAE) and in each case the proposed schemes shows an identical performance.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.174-174
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• 2017

To cope with phosphate (Pi) deficient stress, plants modulate various physiological and developmental processes, such as gene expression, Pi uptake and translocation, and root architecture changes. Here, we report the identification and characterization of novel activation-tagged mutant involved in Pi starvation signaling in Arabidopsis. The hpd (${\underline{h}ypersensitive}$ to ${\underline{P}i}$ $ {\underline{d}eficiency}$) mutant exhibits enhanced phosphate uptake and altered root architectural change under Pi starvation compared to wild type. Expression analysis of auxin-responsive DR5::GUS reporter gene in hpd mutant indicated that auxin translocation in roots under Pi starvation are suppressed in hpd mutant plants. Impaired auxin translocation in roots of hpd mutant was attributable to abnormal root architecture changes in Pi starvation conditions. Our results indicated that abnormal auxin translocation in hpd mutant might be due to mis-regulation of auxin efflux carrier proteins, PIN-FORMED (PIN) 1, and 2 under Pi starvation conditions. Not only expression levels but also expression domains of PIN proteins were altered in hpd mutant in response to Pi starvation. Molecular genetic analysis of hpd mutant revealed that the mutant phenotype is caused by the lesion in ENHANCED SILENCING PHENOTYPE4 (ESP4) gene whose function is proposed in mRNA 3'-end processing. The results suggest that mRNA processing plays crucial roles in Pi homeostasis as well as developmental reprograming in response to Pi deprivation in Arabidopsis.

• Molecules and Cells
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• v.39 no.4
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• pp.322-329
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• 2016

Voltage-gated $Ca^{2+}$ ($Ca_V$) channels are dynamically modulated by Gprotein-coupled receptors (GPCR). The $M_1$ muscarinic receptor stimulation is known to enhance $Ca_V2.3$ channel gating through the activation of protein kinase C (PKC). Here, we found that $M_1$ receptors also inhibit $Ca_V2.3$ currents when the channels are fully activated by PKC. In whole-cell configuration, the application of phorbol 12-myristate 13-acetate (PMA), a PKC activator, potentiated $Ca_V2.3$ currents by ~two-fold. After the PMA-induced potentiation, stimulation of $M_1$ receptors decreased the $Ca_V2.3$ currents by $52{\pm}8%$. We examined whether the depletion of phosphatidylinositol 4,5-bisphosphate ($PI(4,5)P_2$) is responsible for the muscarinic suppression of $Ca_V2.3$ currents by using two methods: the Danio rerio voltage-sensing phosphatase (Dr-VSP) system and the rapamycin-induced translocatable pseudojanin (PJ) system. First, dephosphorylation of $PI(4,5)P_2$ to phosphatidylinositol 4-phosphate (PI(4)P) by Dr-VSP significantly suppressed $Ca_V2.3$ currents, by $53{\pm}3%$. Next, dephosphorylation of both PI(4)P and $PI(4,5)P_2$ to PI by PJ translocation further decreased the current by up to $66{\pm}3%$. The results suggest that $Ca_V2.3$ currents are modulated by the $M_1$ receptor in a dual mode-that is, potentiation through the activation of PKC and suppression by the depletion of membrane $PI(4,5)P_2$. Our results also suggest that there is rapid turnover between PI(4)P and $PI(4,5)P_2$ in the plasma membrane.