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Effects of Soil Water Potential and Nitrogen Fertilization on Characteristics of Photosynthesis and Chlorophyll Fluorescence Induction in Schisandra chinensis Baillon

  • Seo, Young-Jin;Kim, Beung-Sung;Lee, Jong-Phil;Kim, Jong-Su;Park, Kee-Choon;Park, Chun-Geun;Ahn, Young-Sup;Cha, Seon-Woo
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.6
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    • pp.705-711
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    • 2015
  • Management of soil water and fertilization is known to primarily affect physiological properties and yield in plant. The effect of soil water potential and nitrogen application on characteristics of photosynthesis and chlorophyll fluorescence in Schisandra chinensis Baillon was investigated on a sandy loam soil. Net photosyntheis rate and transpiration rate increased as a photon flux density and was highest at -50kPa of soil water potential. Light compensation point ($1.5{\mu}molm^{-1}s^{-1}$) and dark respiration ($0.13{\mu}molCO_2m^{-1}s^{-1}$) was lowest at -50 kPa but maximum photosynthesis rate ($13.10{\mu}molCO_2m^{-1}s^{-1}$) and net apparent quantum yield ($0.083{\mu}molCO_2m^{-1}s^{-1}$) was highest at -50 kPa. As results of chlorophyll fluorescence by OJIP analysis, maximum quantum yield (Fv/Fm) of photosystem II (PSII) and PIabs was higher in treatments of -50 kPa and -60 kPa respectively, which reflects the relative reduction state of PSII. But the relative activities per reaction center such as ABS/RC and DIo/RC were low with decreasing soil water potential. Net photosyntheis rate and transpiration rate were highest at treatment of soil testing 1.0 times ($92kgha^{-1}$). Application of nitrogen resulted in high Fv/Fm, $PI_{abs}$ and low ABS/RC, DIo/RC. This result implies that -50 kPa of soil water potential and nitrogen fertilizer may improve the efficiency of photosynthesis through controlling a photosystem in Schisandra chinensis Baillon.

Study on the Validation of Sasang Digestive Function Inventory (SDFI) for Analyzing Pathophysiological Digestive Symptoms and Diagnosing Sasang Types (소화기 소증 분석과 사상체질 진단을 위한 사상소화기능검사(SDFI)의 타당화 연구)

  • Chae, Han;Lee, Jeongyun;Lee, Yong-jae
    • Journal of Sasang Constitutional Medicine
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    • v.33 no.3
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    • pp.1-15
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    • 2021
  • Introduction The clinical usefulness of Sasang Digestive Function Inventory (SDFI) for analyzing Sasang type-specific pathophysiological symptom was illustrated repeatedly. It was revised to enhance its validity, reliability and clinical usefulness, however its clinical studies supporting these were not sufficient yet. Methods 193 healthy university students responded once to Nepean Dyspepsia Index-Korean (NDIK), Functional Dyspepsia-Related Quality of Life (FDQOL), and Dutch Eating Behavior Questionnaire (DEBQ), and twice to SDFI with four weeks of interval. The Body Mass Index (BMI) and Ponderal Index (PI) were also calculated. The 4-week test-retest reliability and correlation coefficients between NDIK, FDQOL, DEBQ, and SDFI were examined using Pearson's correlation. The significant differences between Sasang types in SDFI and its subscales were examined using ANCOVA with the age as covariate. Results The range of test-retest reliability for SDFI and its subscales was from 0.801 to 0.887. The SDFI-total correlated positively with BMI (r=0.323) and DEBQ-External Eating (r=0.433), while the SDFI-Digestion negatively with NDIK (r=-0.472) and FDQOL-total (r=-0.364). The SDFI-Habit correlated positively with BMI (r=0.310) and DEBQ-total (r=0.481), and the SDFI-Appetite with DEBQ-total (r=0.322). The SDFI-total, SDFI-Digestion, and SDFI-Habit scores of Tae-Eum type (35.6±6.25, 12.38±3.24 and 9.89±3.38, respectively) were significantly bigger than those of So-Eum type (29.84±8.31, 9.93±4.28 and 7.66±3.96). Discussion The SDFI was found to be an objective clinical measure with sufficient concurrent validity for measuring eating behavior and quality of life related to overeating and dyspepsia, and illustrated distinctive differences between Sasang types. It might be useful for the effective medical education and integrative medical practice.

Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells

  • Zhang, Junfang;Li, Qiang;Yan, Yan;Sun, Bin;Wang, Ying;Tang, Lin;Wang, Enze;Yu Jia;Nogoy, Kim Margarette Corpuz;Li, Xiangzi;Choi, Seong-Ho
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.934-953
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    • 2021
  • Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

Study of Anti-inflammatory Activity of 7 Herbal Prescription Effective for Sleep Disorders and Atopic Dermatitis (수면 장애 및 아토피피부염에 효과적인 7 가지 한약 처방의 항염증 활성 연구)

  • Choi, Min-Jin;Nguyen, Ly Thi Huong;Jeong, Jeonghwa;Park, Minyoung;Yoon, Joohee;Lee, Byung-Wook;Yang, In-Jun;Shin, Heung-Mook
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.35 no.2
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    • pp.56-63
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    • 2021
  • This study was conducted to find a candidate prescription with anti-inflammatory efficacy of 7 herbal prescriptions known to be effective in atopic dermatitis and sleep disorders in Korean medicine. The anti-inflammatory of the 7 herbal prescriptions extracts were evaluated by ELISA assay and Western blot assay. 7 herbal prescriptions, Seongyutang(SU), Danseontoetang(DST), Sotosajahwan(ST), Jisiljagyagsan(JJ), Seokchangpo(SCP), Wiryeongseon(WLS), Gogojohwan(GGJ) 30 % EtoH extract was used for in vitro experiments. In TNF-α + IFN-γ(TI) stimulated HaCaT cells, all 7 herbal prescriptions reduced TARC and MDC production, and JJ strongly reduced TARC and MDC production at 100 ㎍/ml concentration. SCP strongly reduced MDC production at 10 ㎍/ml and 100 ㎍/ml concentration. In addition, in substance P(SP)/CRH stimulated HMC-1 cells, JJ strongly inhibited VEGF production at both 10 and 100 ㎍/ml concentrations. In LPS/CRH stimulated Raw264.7 cells, all 7 herbal prescriptions significantly inhibited TNF-α. PMA + Ionomycin(PI)/CRH stimulated EL4 cells, SU significantly reduced IL-4 production at both concentrations of 10 and 100 ㎍/ml. WLS significantly reduced IL-17 production at both concentrations of 10 and 100㎍/ml. This suggests that 7 herbal prescriptions have anti-inflammatory effects by reducing inflammatory cytokines in keratinocytes, mast cells and macrophages caused by inflammation. Therefore, it is expected that 7 herbal prescriptions that are effective for sleep disorders and atopic dermatitis can be used as therapeutic materials for sleep disorders and diseases associated with atopic dermatitis.

The Relationship of Country Image, Product-Country Image, and Purchase Intention of Korean Products: Focusing on Differences among Ethnic Groups in South Africa

  • Lee, You-Kyung;Robb, Charles Arthur
    • Journal of Korea Trade
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    • v.23 no.6
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    • pp.33-51
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    • 2019
  • Purpose - The purpose of this study is to provide practical implications for Korean companies searching for new market opportunities. From the experimental analysis, the impact of country image on product-country image and purchase intention of Korean products are measured. In addition, this study disaggregates the impacts of country image and product-country image and purchase intention among ethnic groups in South Africa for searching further useful implications. Design/methodology - To examine South African consumers' country image and product-country image towards Korean products, data were collected between June and July 2019 through an online questionnaire, and 335 questionnaires were used for analysis. Firstly, the multivariate analysis was conducted to examine the general tendency of South African consumers' perceptions of country image to Korea, product-country image, and purchase intention among three ethnic group consumers. Then in order to verify the country image model and hypotheses of the study, we analyzed the structural models for each of the three ethnic groups and compared the sizes of the path coefficients for each groups. To compare the difference of path coefficients across ethnic groups, configural invariance, metric invariance, and scalar invariance tests were conducted sequentially. Findings - In the black and white ethnic groups, the country image had a statistically significant impact on product-country image, but it did not affect the purchase intention to Korean products. The product-country image showed a statistically significant impact on the purchase intention to Korean products in both ethnic group. However, in the coloured ethnic group consumer, the country image had a significant effect on the product-country image, but it did not affect the purchase intention of Korean products. In addition, the product-country image did not have a significant influence on the purchase intention of coloured ethnic group consumers unlike black and white ethnic group consumers. The results of this study suggest that even though differences in terms of the impact of CoI on PCI and PI were investigated for the sample of white, coloured, and black respondents, the groups seemed to respond in a reasonably comparable manner. Originality/value - South Africa occupies more than 20% of Africa's total GDP in sub-Saharan Africa and is a hub for Southern African logistics as a hub for Korean companies to enter Africa. However, it is rare to find a study focused on the determinants of consumer behavior in South Africa. In particular, this study disaggregates the impacts of country image and product-country image on consumer behavior across ethnic groups in South Africa. Therefore, this study could provide practical implications for Korean firms which desire to diversify their export markets and pioneer future markets.

Effects of Auricular Acupressure on Quality of Sleep and Depression (귀 지압이 수면의 질과 우울증에 미치는 영향)

  • Shin, Hye-Yeon;Jang, Yun-Jeong;Choe, Cheong-Hun;Lee, Da-Hea;Lee, Eun Jin
    • Journal of the Korea Convergence Society
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    • v.12 no.12
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    • pp.509-518
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    • 2021
  • This study was conducted to examine the effects of auricular acupressure on quality of sleep and depression. Adults who had insomnia for over 1 month were recruited. Participants were distributed randomly to the experimental group(n=22) and the control group(n=22). The experimental group received auricular acupressure on Shen men(TF2), insomnia 1(SF4), insomnia 2(SF1), and anti-depression(LO8) for 1 week. The contrast group received sleep hygiene education. The mean differences of the quality of sleep were 4.05±2.52 in the experimental group and 1.45±3.77 in the control group. Auricular acupressure significantly decreased insomnia in the experimental group compared to the control group(t=3.07, p=.004). Depression scores after acupressure were not different between the experimental group and the control group. After controlling the religion as a covariate, the mean difference of depression in the experimental group was higher than the control group(F=4.22, p=.046). In conclusion, auricular acupressure decreased insomnia and depression. Therefore, further studies on different acupressure points such as shen men, jiao gan, heart, pi zhi xia, and endocrine are recommended.

Panax ginseng and its ginsenosides: potential candidates for the prevention and treatment of chemotherapy-induced side effects

  • Wan, Yan;Wang, Jing;Xu, Jin-feng;Tang, Fei;Chen, Lu;Tan, Yu-zhu;Rao, Chao-long;Ao, Hui;Peng, Cheng
    • Journal of Ginseng Research
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    • v.45 no.6
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    • pp.617-630
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    • 2021
  • Chemotherapy-induced side effects affect the quality of life and efficacy of treatment of cancer patients. Current approaches for treating the side effects of chemotherapy are poorly effective and may cause numerous harmful side effects. Therefore, developing new and effective drugs derived from natural nontoxic compounds for the treatment of chemotherapy-induced side effects is necessary. Experiments in vivo and in vitro indicate that Panax ginseng (PG) and its ginsenosides are undoubtedly non-toxic and effective options for the treatment of chemotherapy-induced side effects, such as nephrotoxicity, hepatotoxicity, cardiotoxicity, immunotoxicity, and hematopoietic inhibition. The mechanism focus on anti-oxidation, anti-inflammation, and anti-apoptosis, as well as the modulation of signaling pathways, such as nuclear factor erythroid-2 related factor 2 (Nrf2)/heme oxygenase-1 (HO-1), P62/keap1/Nrf2, c-jun Nterminal kinase (JNK)/P53/caspase 3, mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinases (ERK), AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR), mitogen-activated protein kinase kinase 4 (MKK4)/JNK, and phosphatidylinositol 3-kinase (PI3K)/AKT. Since a systemic review of the effect and mechanism of PG and its ginsenosides on chemotherapy-induced side effects has not yet been published, we provide a comprehensive summarization with this aim and shed light on the future research of PG.

La méthodologie de lecture de la didascalie dans le théâtre contemporain (<현대희곡에서 지문 읽기의 방법적 고찰>)

  • Kim, Young-eun
    • Journal of Korean Theatre Studies Association
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    • no.53
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    • pp.89-128
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    • 2014
  • De notre temps, nous faisons face ${\grave{a}}$ un changement du $th{\hat{e}}atre$ dans son ensemble. Le $th{\acute{e}}atre$ contemporain tente de se $d{\acute{e}}marquer$ des autres formes artistiques, mais aussi des formes $litt{\acute{e}}raires$, lorsque la tendance est ${\grave{a}}$ la fusion des genres. Il existe ainsi de plus en plus de $th{\hat{e}}atre$ ${e}xp{\acute{e}}rimentaux$, $n{\acute{e}}s$ sous le nom de $th{\acute{e}}atre$ anthropologique, $th{\acute{e}}atre$ sociologique, $th{\acute{e}}atre$ ethnologique, $th{\acute{e}}atre$ ${\acute{e}}pist{\acute{e}}miologique$ ou $th{\acute{e}}{\hat{a}}tre$ interculturel. La $m{\acute{e}}thodologie$ doit elle s'adapter ${\grave{a}}$ cette tendance de fusion? Au lieu de trouver une $m{\acute{e}}thode$ d'anlyse de chaque "nouveaux genre" $n{\acute{e}}$ de la fusion, nous nous interrogeons sur la $m{\acute{e}}thodologie$ pour ${\acute{e}}tudier$ le $th{\hat{e}}atre$ dans son ensemble. Si une telle $m{\acute{e}}thodologie$ d'analyse existait, quelle serait la $diff{\acute{e}}rence$ avec la $m{\acute{e}}thodologie$ $d{\acute{e}}j{\grave{a}}$ existante? Et si elle n'existait pas, quelle serait la meilleure $m{\acute{e}}thodologie$ $adapt{\acute{e}}e$? Nous $consid{\acute{e}}rons$ la $m{\acute{e}}thode$ d'analyse de la didascalie comme nouvelle $m{\acute{e}}thodologie$ $adapt{\acute{e}}e$. En effet, la didascalie $conna{\hat{i}}t$ sans cesse des $m{\acute{e}}tamorphoses$ dans $l^{\prime}{\acute{e}}criture$. De nombreux $th{\acute{e}}oriciens$ ont $d{\acute{e}}velopp{\acute{e}}$ des $m{\acute{e}}thodes$ d'analyse afin de mieux comprendre les diverses formes de $th{\acute{e}}atre$ comtemporain et ses didascalies. Notre travail est de rassembler les ${\acute{e}}tudes$ des $diff{\acute{e}}rents$ $th{\acute{e}}oriciens$ et de les ordonner ; afin de proposer un outil d'analyse $adapt{\acute{e}}$ pour $l^{\prime}interpr{\acute{e}}tation$ des $pi{\grave{e}}ces$ du $th{\acute{e}}{\hat{a}}tre$ contemporain.

Variation of Nuclear DNA Content in Interspecific Allium cepa L.×A. fistulosum L. hybrids and Their Successive Backcross Lines (양파와 파간의 종간잡종 F1과 여교잡계통의 핵 DNA 함량)

  • Kim, Cheol-Woo;Kim, Hwa-Young;Lee, Eul-Tai;Choi, In-Hu;Bang, Jin-Ki
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.463-467
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    • 2009
  • Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

Development of a Hydrogen Peroxide Sensor Based on Palladium and Copper Electroplated Laser Induced Graphene Electrode (PdCu를 전기 도금한 레이저 유도 그래핀 전극 기반의 과산화수소 측정 센서 개발)

  • Park, Daehan;Han, Ji-Hoon;Kim, Taeheon;Pak, Jungho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.12
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    • pp.1626-1632
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    • 2018
  • In this paper, we describe the fabrication and characterization of a hydrogen peroxide ($H_2O_2$) sensor based on palladium and copper (PdCu) electroplated laser induced graphene (LIG) electrodes. $CO_2$ laser was used to form LIG electrodes on a PI film. This fabrication method allows simple control of the LIG electrode size and shape. The PdCu was electrochemically deposited on the LIG electrodes to improve the electrocatalytic reaction with $H_2O_2$. The electrochemical performance of this sensor was evaluated in terms of selectivity, sensitivity, and linearity. The physical characterization of this sensor was conducted using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), which confirmed that PdCu was formed on the laser induced graphene electrode. In order to increase the sensor sensitivity, the Pd:Cu ratio of the electroplated PdCu was varied to five different values and the condition of highest amperometric current at an identical of $H_2O_2$ concentration was chosen among them. The resulting amperometric current was highest when the ratio of Pd:Cu was 7:3 and this Pd;Cu ratio was employed in the sensor fabrication. The fabricated PdCu/LIG electrode based $H_2O_2$ sensor exhibited a sensitivity of $139.4{\mu}A/mM{\cdot}cm^2$, a broad linear range between 0 mM and 16 mM of $H_2O_2$ concentrations at applied potential of -0.15 V, and high reproducibility (RSD = 2.6%). The selectivity of the fabricated sensors was also evaluated by applying ascorbic acid, glucose, and lactose separately onto the sensor in order to see if the sensor ourput is affected by one of them and the sensor output was not affected. In conclusion, the proposed PdCu/LIG electrode based $H_2O_2$ sensor seems to be suitable $H_2O_2$ sensor in various applications.