Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study of Anti-inflammatory Activity of 7 Herbal Prescription Effective for Sleep Disorders and Atopic Dermatitis

수면 장애 및 아토피피부염에 효과적인 7 가지 한약 처방의 항염증 활성 연구

  • 최민진 (동국대학교 한의과대학 생리학교실) ;
  • 응웬리 (동국대학교 한의과대학 생리학교실) ;
  • 정정화 ((주)동제메디칼) ;
  • 박민영 (동국대학교 한의과대학) ;
  • 윤주희 (동국대학교 한의과대학) ;
  • 이병욱 ((주)동제메디칼) ;
  • 양인준 (동국대학교 한의과대학 생리학교실) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Received : 2020.12.18
  • Accepted : 2021.04.21
  • Published : 2021.04.25
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Abstract

This study was conducted to find a candidate prescription with anti-inflammatory efficacy of 7 herbal prescriptions known to be effective in atopic dermatitis and sleep disorders in Korean medicine. The anti-inflammatory of the 7 herbal prescriptions extracts were evaluated by ELISA assay and Western blot assay. 7 herbal prescriptions, Seongyutang(SU), Danseontoetang(DST), Sotosajahwan(ST), Jisiljagyagsan(JJ), Seokchangpo(SCP), Wiryeongseon(WLS), Gogojohwan(GGJ) 30 % EtoH extract was used for in vitro experiments. In TNF-α + IFN-γ(TI) stimulated HaCaT cells, all 7 herbal prescriptions reduced TARC and MDC production, and JJ strongly reduced TARC and MDC production at 100 ㎍/ml concentration. SCP strongly reduced MDC production at 10 ㎍/ml and 100 ㎍/ml concentration. In addition, in substance P(SP)/CRH stimulated HMC-1 cells, JJ strongly inhibited VEGF production at both 10 and 100 ㎍/ml concentrations. In LPS/CRH stimulated Raw264.7 cells, all 7 herbal prescriptions significantly inhibited TNF-α. PMA + Ionomycin(PI)/CRH stimulated EL4 cells, SU significantly reduced IL-4 production at both concentrations of 10 and 100 ㎍/ml. WLS significantly reduced IL-17 production at both concentrations of 10 and 100㎍/ml. This suggests that 7 herbal prescriptions have anti-inflammatory effects by reducing inflammatory cytokines in keratinocytes, mast cells and macrophages caused by inflammation. Therefore, it is expected that 7 herbal prescriptions that are effective for sleep disorders and atopic dermatitis can be used as therapeutic materials for sleep disorders and diseases associated with atopic dermatitis.

Keywords

Acknowledgement

본 연구는 2020년도 과학기술정보통신부의 재원으로 한국연구재단 (NO. NRF-2019R1F1A1059856)과 동국대학교의 지원(2020)을 받아 수행된 연구결과입니다.

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