• 한국식품영양학회지
• /
• 제27권2호
• /
• pp.183-193
• /
• 2014

방사선 기술에 대한 오렌지 수입국의 검역 기준을 정립하고, 이를 활용하기 위한 목적의 일환으로 오렌지에 0.4, 0.6, 0.8, 1, 1.5, kGy로 감마선 조사를 실시하여 $20^{\circ}C$에서 15일 동안 저장하면서 미생물 및 이화학적 품질 특성을 조사하였다. 일반 호기성 세균과 효모 및 곰팡이는 비조사구에서 저장기간 증가에 따라 유의적으로 증가하였고, 조사구에서는 선량이 증가할수록 유의적으로 감소하여 미생물 감소 효과를 보였다. Vitamin C 함량은 조사선량과 저장기간의 증가에 따라 유의적으로 감소하였다. 관능평가는 색깔을 제외한 신맛, 단맛, 질감, 향, 전체적인 선호도에서 조사선량과 저장기간이 증가할수록 감소하였고, 향이 가장 낮은 선호도를 보였다. 이상의 결과를 종합하여 볼 때, 감마선 조사는 미생물학적 안정성에는 효과가 있지만, 1 kGy 이상의 감마선 조사는 오렌지의 품질 및 관능 특성에 좋지 않은 영향을 미치는 것으로 보고, 품질을 유지하기 위한 최적 선량은 0.4~0.6 kGy인 것으로 사료된다.

• Food Science and Biotechnology
• /
• 제16권2호
• /
• pp.315-319
• /
• 2007

Biofilm formation on various surfaces is a well-known phenomenon and it has caused pollution problems, health and safety hazards, and substantial economic loss in many areas including the food industry. In the present study, Gamma irradiation at a dose of 2.0 kGy reduced the bacterial counts of Staphylococcus aureus and Pseudomonas aeruginosa suspensions by 6.7 and >6.5 log CFU/mL, respectively, and 30 ppm of sodium hypochlorite effectively reduced the counts of both bacterial suspensions to below the limit of detection ($<2\;log\;CFU/cm^2$). However, in bacterial biofilms attached to stainless steel, gamma irradiation at a dose of 10.0 kGy reduced the counts of S. aureus attached fur 1 hr and overnight by ${\geq}5.1\;and\;5.0\;log\;CFU/cm^2$, respectively. Gamma irradiation at a dose of 1.0 kGy reduced the counts of P. aeruginosa counts to below the limit of detection ($<2\;log\;CFU/cm^2$). On the contrary, S. aureus and P. aeruginosa cells attached to stainless steel chips were difficult to eliminate using sodium hypochlorite. Four hundred ppm of sodium hypochlorite reduced the counts of S. aureus and P. aeruginosa attached for 1 hr by 2.5 and $3.3\;log\;CFU/cm^2$, respectively.

• 약학회지
• /
• 제49권2호
• /
• pp.180-184
• /
• 2005

The purpose of this study was to develop the best methods to sterilize Moutan Root Bark which is frequently used as a herbal medicine, and known to have high susceptibility to m icrobial contamination. We used dry heat, gamma irradiation and alcohol gas treatment for sterilization, and evaluated these methods in terms of the followings; I) the efficacy of sterilization, and ii) the chemical alteration of a major component of the harbal medicines. Treatment with dry heat effectively got rid of contaminated microorganisms, and did not significantly alter the content of paeonol. However it seriously changed the color and morphology which are an essential criterion to estimate a measure of quality of medicinal herbs. Treatment with gamma irradiation showed a strong sterilizing effect, and no alteration of the content of paeonol, color and morphology. Alcohol gas treatment resulted in similar effects as those in gamma irradiation. Collectively, these results suggest that appropriate sterilizing methods to guarantee the microbial quality of this herbal medicine may be those using gamma irradiation or alcohol gas.

• 방사선산업학회지
• /
• 제7권2_3호
• /
• pp.177-182
• /
• 2013

This study was conducted to evaluate the effect of gamma irradiation on the microbial populations of dried raw materials (9 products) and Saengsik powder. The samples were gammairradiated at doses of 2, 4, 6 and 8 kGy and the microbiological populations were evaluated. The total numbers of bacteria and Bacillus cereus in non-irradiated dried-raw materials for Saengsik powder was 1.3~3.4 and $1.7{\sim}2.4log\;CFU\;g^{-1}$. However, gamma irradiation reduced the microbiological populations in all samples, and Saengsik powder were sterilized at more than 6 kGy. Moreover, Clostridium perfringens were not observed in all samples within detection limit (<$1log\;CFU\;g^{-1}$). Therefore, the results of this study suggest that gamma irradiation at 6 kGy is sufficient to sterilize Saengsik powder, and thus, irradiated Saengsik powder at 6 kGy fulfills the microbiological requirements for sterilized food.

• 방사선산업학회지
• /
• 제6권3호
• /
• pp.211-215
• /
• 2012

In this study, crosslinked polyethylene (PE) foam sheets were prepared through gamma-ray irradiation. PE foam sheets were prepared through a foaming process using sodium bicarbonate as a blowing and foaming agent. The prepared PE foam sheets were then crosslinked through gamma-ray irradiation. The crosslinking degree was increased to 86.0% with an increase in the absorption dose. The tensile strength of the crosslinked PE foam sheets was increased with an increase in the absorption dose. However, the elongation-at-break of the crosslinked PE foam sheets was decreased. The thermal decomposition temperature of the crosslinked PE foam sheets was increased to $421.2^{\circ}C$ with an increase in the absorption dose. The SEM analysis revealed that the morphology was not changed significantly after the crosslinking through gamma-ray irradiation.

• 방사선산업학회지
• /
• 제6권3호
• /
• pp.239-244
• /
• 2012

This study was conducted to investigate bacterial growth, viscosity, color, and sensory properties of gamma-irradiated Tarakjuk powder, a Korean milk porridge powder, at 1, 3, 5, 7, and 10 kGy. The total aerobic bacteria in non-irradiated Tarakjuk powder was $2.56{\log}\;CFU{\cdot}g^{-1}$, whereas it was not observed within the detection limit of $2{\log}\;CFU{\cdot}g^{-1}$ in samples irradiated at more than 1 kGy. Spore-forming bacteria, however, were not observed in all samples within the detection limit of $1{\log}\;CFU{\cdot}g^{-1}$. The viscosity of rehydrated Tarakjuk after gamma irradiation significantly decreased from 16,770 cP to 4,060 cP when irradiated at 10 kGy. The redness ($a^*$ value) and yellowness ($b^*$ value) evaluated using a colorimeter were significantly increased according to the increase in irradiation dose (p<0.05), while there was no difference in color evaluation conducted by panels. The overall acceptance decreased as the irradiation dose increased, and the 5 kGy sample was 4.0 (normal) on a 7-point scale. As a result, it is considered that a gamma irradiation of 5 kGy is enough to sterilize Tarakjuk powder with a acceptable sensory quality.

• 한국식품저장유통학회지
• /
• 제30권5호
• /
• pp.729-742
• /
• 2023

The consumption of king oyster mushrooms has steadily increased owing to their unique flavor and delicate texture. This study extended the storage period of king oyster mushrooms (Pleurotus eryngii ) via gamma irradiation. Irradiated samples (0, 0.5, 1, 2, and 3 kGy) were stored at 4℃ with 80% relative humidity for up to 28 days, and the experiments were conducted every 7 day. Microbiological analysis revealed a dose-dependent reduction in total aerobic bacteria, Pseudomonas spp., and yeasts and molds, and gamma irradiation above 2 kGy effectively controlled microbial contamination for up to 28 days. At the 28th day of storage, the irradiated king oyster mushrooms exhibited delayed browning through the reduction of tyrosinase activity. Moreover, firmness reduction (%) was 80.59±1.89% for the non-irradiated group and 42.80±1.28, 34.57±1.13, 31.05±3.24, and 39.73±0.94% for the irradiated group (0.5, 1, 2, and 3 kGy), respectively. These results were supported by the scanning electron microscopy photos, which showed smaller pores in the irradiated group than in the non-irradiated group. This study demonstrated that 2 kGy of gamma irradiation effectively reduces microbial contamination and delays the browning and softening of king oyster mushrooms for up to 28 days.

• Nuclear Engineering and Technology
• /
• 제56권5호
• /
• pp.1803-1812
• /
• 2024

The attractive properties of gadolinium-based nanoparticles as a positive contrast agent for magnetic resonance imaging (MRI) have piqued the interest of both researchers and clinicians. Nonetheless, due to the biotoxicity of gadolinium (III) ions' free radicals, there is a need to address this issue. Therefore, this research aimed to develop a biocompatible, dispersible, stable, hydrophilic, and less toxic cellulose nanocrystals/gadolinium oxide nanocomposite as contrast agent properties for MRI purposes. This study aimed to synthesize gadolinium oxide nanoparticles coated with cellulose nanocrystals with polyethylene glycol and sodium hydroxide (CNCs-PEG/NaOH)/Gd₂O₃ using the gamma irradiation method to reduce the particle size. The results showed that using a gamma irradiation dose of 10 kGy, quasi-spherical morphology with a size of approximately 5.5 ± 0.65 nm could be produced. Furthermore, the cytocompatibility of (CNCs-PEG/NaOH)/Gd₂O₃ nanocomposite synthesized was assessed through MTT assay tests on Hep G2 cells, which demonstrated good cytocompatibility without any cytotoxic effects within a concentration range of (10 ㎍/mL - 150 ㎍/mL) and had sufficient cellular uptake. Moreover, the T₁-weighted MRI of (CNCs-PEG/NaOH)/Gd₂O₃ nanocomposite revealed promising results as a positive contrast agent. It is envisaged that the gamma irradiation method is promising in synthesizing (CNCs-PEG/NaOH)/Gd₂O₃ nanocomposite with nanoscale for different applications, especially in the radiotherapy field.

• 대한수의학회지
• /
• 제34권4호
• /
• pp.665-678
• /
• 1994

This study was undertaken to investigate histological changes according to the radiosensitivity in the spermatogenic cells in Korean native pheasants. During spermatogenetic period, testes wete collected from male adult Korean native pheasant and they were used as experimental and control birds. The experimental group was divided into a single-dose whole body irradiation group(400, 600, 800 and 1000 rads) and a split-dose whole body irradiation groups(400/2, 600/2, 800/2 and 1000/2 rads). A Henseky's $^{60}Co$ ${\gamma}$-radiotherapy machine was used for this experiment and the dose rate of $^{60}Co$ ${\gamma}$-ray was 104 rads/min. The experimental birds were sacrificed at 24 and 72 hrs after irradiation and the control pheasants were sacrificed at the same time. General histological changes of seminiferous epithelial cells were observed by hematoxylin-eosin stain with light microscope. The results obtained are summarized as follows ; 1. In the single-dose and the split-dose irradiation groups, the average diameter of the seminiferous tubule was decreased compared with control group. 2. Seminiferous epithelial cells were more severely damaged after 72 hrs than after 24 hrs of single-dose irradiation of 400, 600 and 800 rads but the difference of cell injury was almost not observable with the elapsed time in the group of the single-dose irradiation of 1000 rads. 3. The damage of spermatogenic cells were more severe after 24 hrs than after 72 hrs of the split-dose irradiation of 400 rads but the split-dose irradiation of 600, 800 and 1000 rads were more severe after 72 hrs than after 24 hrs.

• Journal of Ginseng Research
• /
• 제22권4호
• /
• pp.252-259
• /
• 1998

Electron beam, electrically produced from an electron accelerator, was compared with gamma energy in terms of its influence on color and organoleptic qualities of ginseng powders when exposed to the energy used for their microbial decontamination. Hunter color L and b values were suitable for measuring color characteristics of ginseng powders, which were not significantly changed by the exposure to 5 to 7.5 kGy electron beam and gamma energy. Fifty percent ethanol extracts of irradiated ginseng powders at 10 key showed negligible differences from the non-irradiated control in the pattern of absorption spectra at 280∼800 am, but showed increased values in overall color difference (AE) as compared with powdered samples. Irradiation more than 10 kGy and storage at ambient temperature for 4 months caused browning of powdered samples. Irradiation at more than 10 kGy of electron beam was found a critical level to bring about appreciable changes (p<0.05) in or-ganoleptic qualities such as color and odor of sterilized samples, and red ginseng powder was more susceptible than white one to organoleptic changes by irradiation.