The phenomenon of vocal vibrato may be regarded as an acoustic representation of one of the most rapid and continuous changes in pitch and intensity that the human vocal mechanism is capable of producing. Singers are likely to use vibrato effectively to enrich their voice. The purpose of this study was to obtain acoustic measurements (vF0 and vAm) of 45 subjects (15 trot and 15 ballad singers and 15 non-singers) and to compare acoustic measurements of the vowel /a/ produced by 3 groups on 2 voice sampling conditions (prolongation and singing of /a/). Thirty singers of trot and ballad were selected by a producer and a concert director working for the KBS (Korean Broadcasting System). The MDVP was used to measure the acoustic parameters. A two-way MANOVA was used for statistical analyses. The results were as follows; Firstly, there was no significant difference among the 3 groups in vF0 and vAm in prolongation of /a/, but in singing voice, there was a significant difference among 3 groups in vF0 and vAm. Secondly, there was an interaction between music genre and voice sampling condition in vF0, and vAm. Finally, trot singers sing with more vibrato than ballad singers. It was concluded that it is very important to analyze singers' voice including various voice conditions (prolongation, reading, conversation, and singing) and to identify differences of singing voice characteristics among music genre.