In modal analysis, the mode shape reflects the vibration characteristics of the structure, and thus it is widely performed for finite element model updating and structural health monitoring. Generally, the acceleration-based mode shape is suitable to express the characteristics of structures for the translational vibration; however, it is difficult to represent the rotational mode at boundary conditions. A tilt sensor and gyroscope capable of measuring rotational mode are used to analyze the overall behavior of the structure, but extracting its mode shape is the major challenge under the small vibration always. Herein, we conducted a feasibility study on a multi-mode shape estimating approach utilizing a single physical quantity signal. The basic concept of the proposed method is to receive multi-metric dynamic responses from two sensors and obtain mode shapes through bridge loading test with relatively large deformation. In addition, the linear transformation matrix for estimating two mode shapes is derived, and the mode shape based on the gyro sensor data is obtained by acceleration response using ambient vibration. Because the structure's behavior with respect to translational and rotational mode can be confirmed, the proposed method can obtain the total response of the structure considering boundary conditions. To verify the feasibility of the proposed method, we pre-measured dynamic data acquired from five accelerometers and five gyro sensors in a lab-scale test considering bridge structures, and obtained a linear transformation matrix for estimating the multi-mode shapes. In addition, the mode shapes for two physical quantities could be extracted by using only the acceleration data. Finally, the mode shapes estimated by the proposed method were compared with the mode shapes obtained from the two sensors. This study confirmed the applicability of the multi-mode shape estimation approach for accurate damage assessment using multi-dimensional mode shapes of bridge structures, and can be used to evaluate the behavior of structures under ambient vibration.