Recently, habitat fragmentation and shrinkage has occurred because of increased road construction. As a measures mitigating the adverse effect such as eco-island by road construction, Korea has been installed eco-corridors since 1995. Using the Analytic Hierarchy Process (AHP), this study analyzed the importance of assessment items and the criteria to determine which areas require eco-corridors the most. First, related literature reviews, preliminary surveys, and expert interviews were carried out to develop assessment items and criteria for constructing the hierarchy. Second, experts were surveyed in order to determine the relative importance of the assessment items by applying the AHP. As a result, it found that the restoration of the ecological network was the most important assessment item, followed by wild animal resources in the Level 3. In the Level 4, the eighteen items were ranked in the order of their relative weight and it was found that the 'Baekdudaegan Mountain Range (0.189)' was the highest ranked item. Therefore, this approach can be applied to effective selection of the priority areas in planning eco-corridors in the national scale.