Today, the power capacity of a wind turbine and the size of a blade is increasing to capture more wind resources, reduce the number of wind turbines on a wind farm, and reduce the cost of energy. As the blade size becomes larger, attention is being paid to the structural integrity of the blade root connection due to the heavy gravitational load effect and increased aerodynamic loads on the large blade, which could cause catastrophic failure of the blade. Therefore, the secure bolted joint connection of the blade to the hub is very important. In this paper, attention was given to the stress concentration factor (SCF) at the first thread between the M42 bolt and nut. The effect of various design parameters on the stress concentration factor was investigated, which included nut type, nut height, and reduced shank bolt. From a close design investigation of the numerical results, it turned out that the use of a reduced shank bolt resulted in the largest reduction of the stress concentration factor by 40 %, and the round nut type also reduced the SCF by 10 %, which will be beneficial to large wind turbine blades over 100 meters.