Kim, Jung Min;Ryu, Jaihyunk;Lee, Min-Kyu;Kim, Dong-Gun;Hong, Min Jeong;Kim, Jin-Baek;Kang, Si-Young;Ahn, Joon-Woo;Ha, Bo-Keun;Kwon, Soon-Jae
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This study was carried out to examine radio-sensitivity to gamma-rays irradiated at different doses and to determine optimal dose for mutation breeding in sorghum [Sorghum bicolor(L.) Moench]. Ten cultivars were irradiated with gamma-rays at 100, 200, 300, 400, 500, 600, 700, and 1000 Gy. The germination rate of $M_1$ seeds was gradually decreased at doses higher than 300 Gy and showed no germination over 600 Gy. Likewise, the survival rate of $M_1$ seedling decreased at doses higher than 200 Gy and there were no survival over 500 Gy in all cultivars. However, the median lethal doses($LD_{50}$) and the median reduction doses($RD_{50}$) values of the tested sorghum cultivars were slightly different between cultivars. The $LD_{50}$ was 240(DINE-A-M), 225(IS225), 335(IS2868), 180(Moktak), 214(Banwoldang), 213(ChalI), 231(ChalII), 182(KLSO79125), 287(KLSO79075), and 274(Mesusu) Gy. The $RD_{50}$ was 179(DINE-A-M), 175(IS225), 210(IS2868), 142(Moktak), 174(Banwoldang), 163(ChalI), 175(ChalII), 159(KLSO79125), 199(KLSO79075), and 195(Mesusu) Gy depending on the plant height. Afterwards, we confirmed growth characteristics and mutants of the $M_2$ generation and then were collected mutant lines better than original cultivars. As a result, we suggest that the optimal doses of gamma-ray irradiations for mutation breeding of sorghum are 180 Gy(Moktak, KLSO79125), 200 Gy(DINE-A, IS645, Banwoldang, ChalI, ChalII), 250 Gy(KLSO79075, Mesusu), and 300 Gy(IS2868). By irradiation of gamma-rays at the suggested optimal doses, we constructed $M_2$ lines for sorghum breeding.