Allergy, Asthma & Respiratory Disease

  • 제12권3호
  • /
  • Pages.147-154
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  • 2024
  • /
  • 2288-0402(pISSN)
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  • 2288-0410(eISSN)
대한천식알레르기학회 (The Korean Academy of Asthma, Allergy and Clinical Immunology)
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소아 갈치알레르기: 대구 특이 IgE 검사 활용성 및 대구와의 교차반응

Cutlassfish allergy in children: Usefulness of serum cod specific IgE and cross-reaction with cod

  • 염상화 (아주대학교 의과대학 소아청소년과학교실) ;
  • 프레브산 간툴가 (아주대학교 의과대학 소아청소년과학교실) ;
  • 정경욱 (아주대학교 의과대학 소아청소년과학교실) ;
  • 박경희 (연세대학교 의과대학 내과학교실) ;
  • 박중원 (연세대학교 의과대학 내과학교실) ;
  • 이수영 (아주대학교 의과대학 소아청소년과학교실)
  • Sanghwa Youm (Department of Pediatrics, Ajou University College of Medicine) ;
  • Purevsan Gantulga (Department of Pediatrics, Ajou University College of Medicine) ;
  • Kyunguk Jeong (Department of Pediatrics, Ajou University College of Medicine) ;
  • Kyung Hee Park (Department of Internal Medicine, Yonsei University College of Medicine) ;
  • Jung-Won Park (Department of Internal Medicine, Yonsei University College of Medicine) ;
  • Sooyoung Lee (Department of Pediatrics, Ajou University College of Medicine)
  • 투고 : 2024.01.27
  • 심사 : 2024.02.19
  • 발행 : 2024.07.30
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초록

Purpose: Fish allergy is the ninth common food allergy, and cutlassfish is one of the common allergenic fishes in Korean children. However, there is no commercial diagnostic tool for testing cutlassfish allergy in the world. We evaluated the usefulness of serum cod specific IgE (cod-sIgE) to diagnose cutlassfish allergy and cross-reaction between cutlassfish and cod. Methods: Nineteen children who experienced immediate type reactions after consumption of cutlassfish were enrolled. Cod-sIgE was measured by ImmunoCAP, and serum samples were obtained from 11 allergic patients and 11 controls. Using our own homemade crude extracts, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), anti-parvalbumin (PV) immunoglobulin G immunoblot, enzyme-linked immunosorbent assay (ELISA), and ELISA inhibition were performed. Results: Thirteen patients were clinically allergic to both cutlassfish and cod, and 6 were allergic to cutlassfish alone. The median age and cod-sIgE concentrations were not significantly different between the 2 groups. The clear fish protein bands and PVs were identified on SDS-PAGE and immunoblotting. Serum cod-sIgE was positive in 4 out of 6 cutlassfish mono-allergic patients, however, there was no significant correlation between cod-sIgE by ImmunoCAP and cutlassfish-specific IgE by ELISA. The cutlassfish IgE ELISA was profoundly inhibited by cutlassfish, while the cod IgE ELISA was profoundly inhibited by cod but partially inhibited by cutlassfish. Conclusion: We found a potential diagnostic value of cod-sIgE to diagnose cutlassfish allergy and the asymmetric cross-reaction between cutlassfish and cod. These results could help diagnose and provide a dietary guidance in cutlassfish allergic children.

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참고문헌

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