Journal of Microbiology and Biotechnology

  • 제34권10호
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  • Pages.2023-2032
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Screening of Antagonistic Bacteria against Three Aquatic Pathogens and Characterization of Lipopeptides in Bacillus cereus BA09

  • Xinran Shi (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Weijia Zhou (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Xiaocen Lu (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Cuiyan Cao (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Key Laboratory of Separation Science for Analytical Chemistry) ;
  • Dong Sheng (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Xu Ren (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Nanlin Jin (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Yu Zhang (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Zhixin Guo (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Shengnan Cao (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University) ;
  • Shigen Ye (Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University)
  • 투고 : 2024.04.11
  • 심사 : 2024.08.05
  • 발행 : 2024.10.28
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초록

Screening for antagonistic bacteria on aquatic pathogens and identification of antagonistic ingredients are essential to reduce the use of chemicals in aquaculture. In this study, strain BA09, subsequently identified as Bacillus cereus, simultaneously displayed strong antagonistic effects on Edwardsiella tarda, Vibrio harveyi, and Streptococcus anisopliae in the initial screening and rescreening. In addition, the methanol extract of BA09 was subjected to antibacterial activity verification and one-dimensional (1D) reversed-phase liquid chromatography (RPLC) preparation. A total of 27 fractions were collected, 6 of which were subjected to two-dimensional (2D) RPLC separation and tracked as antibacterial. A total of 14 lipopeptides that included 9 fengycin homologs, 3 bacillomycin homologs, and 2 surfactin homologs were identified by tandem high-resolution mass spectrometry. Through characterization of the antibacterial substance in Bacillus cereus BA09, which simultaneously inhibited E. tarda, V. harveyi, and S. agalactiae, the current study provides a theoretical basis for the development of antibacterial drugs in aquaculture.

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과제정보

The work was supported by Liaoning Provincial Science and Technology Mission Project (20230025), Liaoning Province Doctoral Scientific Research Foundation Project (2021-BS-236,2021-BS-238) and Liaoning Provincial Department of Education Basic Scientific Research (JYTMS20230485).

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