Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Sodium Hydrosulfide Enhances Drought Tolerance by Alleviating Oxidative Stress and Promoting Proline Accumulation in Brassica napus L.

  • Septi Anita Sari (Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Muchamad Muchlas (Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Bok-Rye Lee (Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Md Al Mamun (Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Tae-Hwan Kim (Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University)
  • Received : 2024.09.11
  • Accepted : 2024.09.24
  • Published : 2024.09.30
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Abstract

Drought is one of the environmental factors inhibiting plant productivity and growth, leading to oxidative damage. This study aims to identify the role of sodium hydrosulfide (NaHS) as a hydrogen sulfide (H2S) donor in drought stress tolerance in Brassica napus. Drought-induced stress symptoms appeared eight days after treatment, showing wilted leaves and a significant reduction of leaf water potential. Drought-induced increase of lipid peroxidation was significantly reduced by NaHS application. NaHS-treated plants mitigated stress symptoms under drought conditions by reducing hydrogen peroxide (H2O2) content, confirmed with H2O2 localization in situ. Furthermore, NaHS promotes photosynthetic activity by maintaining chlorophyll and carotenoid content, thereby supporting plant growth under drought conditions. Pyrroline-5-carboxylate and proline contents were significantly increased by drought but further enhanced by NaHS treatment, indicating the important roles of proline accumulation in drought stress tolerance. In conclusion, this study provides valuable insight into the roles of NaHS in alleviating drought stress by reducing oxidative stress and promoting proline accumulation. Therefore, NaHS may serve as an effective strategy to enhance crop production under drought-stress conditions.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2022R1I1A3072357).

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