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Changes in protein content and bioactive activity in Phellinus linteus HN00K9 cultured on oat

목질진흙버섯HN00K9 배양 귀리의 단백질함량 및 생물활성 변화

  • Ja-Yoon Kim (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hee-Wan Kang (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University)
  • 김자윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 강희완 (한경국립대학교 생명공학부)
  • Received : 2024.08.05
  • Accepted : 2024.09.23
  • Published : 2024.09.30

Abstract

This study aimed to analyze crude protein, amino acid and bioactive changes in Phellinus linteus HN00K9 cultured oat. The crude protein content of P. linteus cultured oat (PCO) was 12.9%, which was higher than that of uncultured oats (UCO) as control at 11.26%. The total free amino acid contents of PCO and UCO were 11,4 mg/100 g and 9,686.205 mg/100 g, respectively. Glutamic acid accounted for 21% of the total amino acids of PCO, and the histidine content was increased by more than 51% in PCO compared to UCO. The total polyphenol content of PCO was 275 mg GAE/g, which was more than twice that of UCO (135 mg). The DPPH radical scavenging activity was 15.5% in PCO, which was more than five times that of UCO (3.5%). The β-glucan content of PCO was 12.5 g/100 g, which was more than five times that of UCO (3.2 g/100 g). Therefore, it is believed that PCO can be utilized as a material for various functional foods.

목질진흙버섯 HN00K9 배양 귀리(PCO)의 조단백질 함량은 12.9%로 배양되지 않은 귀리(UCO)의 11.26%보다 높았으며 총 유리 아미노산 함량은 각각 11,429 mg과 9,686 mg/100 g였으며 글루탐산은 PCO의 총 아미노산의 21%를 차지하였으며 히스티딘 함량은 UCO에 비해 PCO에서 51% 이상 증가했다. PCO의 총 폴리페놀 함량은 275 GAE/g로 UCO의 135 mg 보다 2배 이상 높았다. DPPH 라디칼 소거능은 PCO에서 15.5%였으며, UCO의 3.5%에 비해 5배 이상의 활성을 보였다. PCO의 β-글루칸 함량은 12.5 g/100 g로 UCO의 3.2 g/100 g에 비해 5배 이상 증가하였다. 따라서 목질진흙버섯 HN00K9 배양 귀리는 다양한 기능성 식품 소재로 활용될 수 있을 것으로 사료 된다.

Keywords

Acknowledgement

본 연구는 과학기술정보통신부 연구개발특구진흥재단 민간 투자형 R&BD (과제번호: 2022-DD-RD-0574-02) 지원으로 수행된 연구 결과이며 연구비 지원에 감사드립니다.

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